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The map label for this gene is rluB [H]

Identifier: 52143877

GI number: 52143877

Start: 1448238

End: 1448966

Strand: Direct

Name: rluB [H]

Synonym: BCZK1354

Alternate gene names: 52143877

Gene position: 1448238-1448966 (Clockwise)

Preceding gene: 52143876

Following gene: 52143874

Centisome position: 27.32

GC content: 38.68

Gene sequence:

>729_bases
ATGGAACGATTACAAAAAGTGATTGCGCAAGCAGGTATTGCATCGAGAAGAAAGGCTGAGGAATTAATTCAGCAAGGAAA
AGTGAAAGTTAACGGAAAAATAGTGAAGGAGTTAGGAACGAAAGTAGCTCCTCAAGATAAAGTAGAAGTAAATAACATCC
CTCTTGAAAAAGAAGAACCTGTTTATTTTTTACTATATAAACCAACTGGCGTAATTTCAAGTGTATCAGATGATAAAGGA
AGAAGTGTTGTAACAGACTTTTTCCCGGAAATTACACAACGTTTATTCCCAATCGGACGCTTAGATTATGATACGTCTGG
TGTATTATTAATGACAAACGATGGGGACTTCGCAAACGTATTAATGCACCCTAGATATAAAGTTGAAAAAACATATGTTG
CAAAAATAAAAGGCCCACTAACAGGTGAGAAAATCCGCATGTTAGAACGTGGTGTGATGTTAGAAGACGGTAAAACAGCA
CCAGCGCGTGTGAAGATTATTTCTTGGGACAAGCGTAAAGAAATGGCGATTGTACAATTAACAATTCACGAGGGACGTAA
CCGTCAAGTACGTCGTATGTTTGAAGCGTTAGATTGTAAAGTAGTGAAACTAAAGCGTGAACGCTATGCTTTCTTAGAAG
TAGGAAGCCTACGACCTGGTGATGCAAGAGAATTGACACCACATGAGGTAAAACAATTACGTGCATTGGCGTCTACAAAG
CCTCGCTAA

Upstream 100 bases:

>100_bases
CTATGTTGTTTAAGTAAGATGAAGCGAAAAGCCGTCAAGCGTATGCGGTTGCCAAGTCTTTCTAATGTAAGGTAAGATAA
ATTCGAGGTGAAAAAAAGAA

Downstream 100 bases:

>100_bases
TCGATATTTTGAAAATTTCACCAGTGGAATGCTCCGCTGGTGATTTATTAAAAAATACATATATTCATCACTCGTCGAAG
AGAGGGGAGACAATATGAAA

Product: pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MERLQKVIAQAGIASRRKAEELIQQGKVKVNGKIVKELGTKVAPQDKVEVNNIPLEKEEPVYFLLYKPTGVISSVSDDKG
RSVVTDFFPEITQRLFPIGRLDYDTSGVLLMTNDGDFANVLMHPRYKVEKTYVAKIKGPLTGEKIRMLERGVMLEDGKTA
PARVKIISWDKRKEMAIVQLTIHEGRNRQVRRMFEALDCKVVKLKRERYAFLEVGSLRPGDARELTPHEVKQLRALASTK
PR

Sequences:

>Translated_242_residues
MERLQKVIAQAGIASRRKAEELIQQGKVKVNGKIVKELGTKVAPQDKVEVNNIPLEKEEPVYFLLYKPTGVISSVSDDKG
RSVVTDFFPEITQRLFPIGRLDYDTSGVLLMTNDGDFANVLMHPRYKVEKTYVAKIKGPLTGEKIRMLERGVMLEDGKTA
PARVKIISWDKRKEMAIVQLTIHEGRNRQVRRMFEALDCKVVKLKRERYAFLEVGSLRPGDARELTPHEVKQLRALASTK
PR
>Mature_242_residues
MERLQKVIAQAGIASRRKAEELIQQGKVKVNGKIVKELGTKVAPQDKVEVNNIPLEKEEPVYFLLYKPTGVISSVSDDKG
RSVVTDFFPEITQRLFPIGRLDYDTSGVLLMTNDGDFANVLMHPRYKVEKTYVAKIKGPLTGEKIRMLERGVMLEDGKTA
PARVKIISWDKRKEMAIVQLTIHEGRNRQVRRMFEALDCKVVKLKRERYAFLEVGSLRPGDARELTPHEVKQLRALASTK
PR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=249, Percent_Identity=39.3574297188755, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1788510, Length=236, Percent_Identity=36.864406779661, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1790453, Length=237, Percent_Identity=35.8649789029536, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87081838, Length=184, Percent_Identity=30.9782608695652, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27527; Mature: 27527

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLQKVIAQAGIASRRKAEELIQQGKVKVNGKIVKELGTKVAPQDKVEVNNIPLEKEEP
CHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCEEHHHHCCCCCCCCCEEECCCCCCCCCC
VYFLLYKPTGVISSVSDDKGRSVVTDFFPEITQRLFPIGRLDYDTSGVLLMTNDGDFANV
EEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEE
LMHPRYKVEKTYVAKIKGPLTGEKIRMLERGVMLEDGKTAPARVKIISWDKRKEMAIVQL
EECCCEEECCEEEEEECCCCCHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCCEEEEEE
TIHEGRNRQVRRMFEALDCKVVKLKRERYAFLEVGSLRPGDARELTPHEVKQLRALASTK
EEECCCCHHHHHHHHHHCCEEEEEHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
PR
CC
>Mature Secondary Structure
MERLQKVIAQAGIASRRKAEELIQQGKVKVNGKIVKELGTKVAPQDKVEVNNIPLEKEEP
CHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCEEHHHHCCCCCCCCCEEECCCCCCCCCC
VYFLLYKPTGVISSVSDDKGRSVVTDFFPEITQRLFPIGRLDYDTSGVLLMTNDGDFANV
EEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEE
LMHPRYKVEKTYVAKIKGPLTGEKIRMLERGVMLEDGKTAPARVKIISWDKRKEMAIVQL
EECCCEEECCEEEEEECCCCCHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCCEEEEEE
TIHEGRNRQVRRMFEALDCKVVKLKRERYAFLEVGSLRPGDARELTPHEVKQLRALASTK
EEECCCCHHHHHHHHHHCCEEEEEHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
PR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]