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The map label for this gene is rnjA [H]

Identifier: 52143749

GI number: 52143749

Start: 1577774

End: 1579447

Strand: Direct

Name: rnjA [H]

Synonym: BCZK1484

Alternate gene names: 52143749

Gene position: 1577774-1579447 (Clockwise)

Preceding gene: 52143750

Following gene: 52143748

Centisome position: 29.76

GC content: 33.57

Gene sequence:

>1674_bases
TTGAAGTCATTAAAAAACAACTTGTCTATCGTTGCTTTAGGTGGAGTAAATGAAATAGGGAAAAATATGTATGCTATTCA
ATATGAAAATGATATTGTGGTTATTGATTGCGGATCTAAATTTCCAGATGAAAGTTTATTAGGAATTGATTTAATCATTC
CAGACGTCACATACTTACAAGAAAATAAAGAAAAAATTCGCGGATTAGTAGTAACGCATGGACATGAAGATCATATTGGC
GGAATACCATATTTTTTAAAACAACTAAACGTACCAATCTATGCGACGAAGTTAACGTTAGGTTTAATTGAAATTAAATT
AAAGGAACATAACCTTCAAAATGATACAGAATTAATCGTTATTCATTCAGAATCAGAAATTGATTTTGGTTCTATTAAGA
CGACTTTTTTTAAAACGAATCATAGCATTCCAGATTGCCTTGGTATTGCTTTCCATACACCAGAAGGTACTGTAGTACAT
ACAGGTGATTTTAAATTTGATTTAACACCAGTAAATAACCAACAGCCTGATATTCATAAAATGGCTAAAATAGGTAGTGA
AGGCGTGCTAGCTTTATTATCTGAAAGTACAAATGCAGAACGACCAGGATTTACTCCATCAGAAAGGTCGGTAGGGGAAC
GGGTAGAAGAAATATTTATGAAAGCAAATAGGAAAGTGATTATTTCTACTTTTGCTTCTAATGTGAATCGTGTTCAGCAA
ATAGTTGAAGCTTGTATAAAAACAAATCGAAAACTGGCTTTACTCGGGAGAAGTATGGTAAATGTAGTAGAAGTTGCTCT
AGAGAAAGGGTATCTAAATATTCCAGATGGTATGTTAATTGAAGCAAGTGAGGTAAATCGTTTAGATCCAAAGCAGGCTG
CAATCCTTTGTACAGGAAGCCAAGGAGAACCAATGGCAGCTTTAGCGCGGTTAGCTAGTGGAAACTATAGGCAAGTGGAT
GTTTTACCAGAAGATACGGTTATTATAGCGGCAACGCCTATTCCGGGAAATGAACGTAACGTTTCACGAATTATAGATAA
TTTATTCGCATTAGGAGCAAAAGTAATTTACGGATCAGGTAGTTCTACTGGAGTCCATGTATCTGGTCATGCGTATCAAG
AAGAATTGAAATTAATGCTTGCTTTAATGAAGCCAAAATATTTTATCCCTATTCATGGAGAATTTAGAATGCTACATCAC
CATAGTTTGTTAGCAGAATCAATTGGAGTTAAAAAAGAGAATATTTTTATCGTTCGTAATGGAGATGTTGTAGATATAAG
TAATGAAGTGGCAATCCAATCTAGAAAAATACAGGCTGGAAATATATATGTAGATGGATTAGGAATTGGTGACGTTGGAA
ATGCCTTATTACGTGATCGAAAACAACTTTCAGAAGATGGAATGCTCGTTATAGTTATTACTTTTAATAAAGCGGATGGA
GATATAATTTCAGGTCCTGATATTATATCTCGTGGATTTGTCTATGTTCGTGATTCAGAAGAATTTTTGAAGGAATTAAA
TAAATTAGCTGTTATTACGATTAATGATTTAAAGAAAGAGAAAGTGAATAGCTGGGGTATTTTGAAAAGAGAAGTAAGAG
AAGCTTTAGGGAAGTATATATATACGAATACGAAAAGAAAGCCGATGATTCTTCCTATAATAATAGAGGTGTAA

Upstream 100 bases:

>100_bases
TTTTTTATGTATGAAACAGTATGAAGTCCTTTTCTATTACAAATAATAAAGCCTAAATACGATTTCACGAATTATAACAG
TGAAAGGGAGGGTTTATAAT

Downstream 100 bases:

>100_bases
TAATGAATAAAAAGAATTCTCGTATGGGAATTCTTTTTTTGTTTTGAAGATAGAATGAACATATAATGAAGTAAATAGGA
ATGGAAGGGAATAAAAGATG

Product: Zn-dependent hydrolase

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MKSLKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDVTYLQENKEKIRGLVVTHGHEDHIG
GIPYFLKQLNVPIYATKLTLGLIEIKLKEHNLQNDTELIVIHSESEIDFGSIKTTFFKTNHSIPDCLGIAFHTPEGTVVH
TGDFKFDLTPVNNQQPDIHKMAKIGSEGVLALLSESTNAERPGFTPSERSVGERVEEIFMKANRKVIISTFASNVNRVQQ
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLNIPDGMLIEASEVNRLDPKQAAILCTGSQGEPMAALARLASGNYRQVD
VLPEDTVIIAATPIPGNERNVSRIIDNLFALGAKVIYGSGSSTGVHVSGHAYQEELKLMLALMKPKYFIPIHGEFRMLHH
HSLLAESIGVKKENIFIVRNGDVVDISNEVAIQSRKIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKADG
DIISGPDIISRGFVYVRDSEEFLKELNKLAVITINDLKKEKVNSWGILKREVREALGKYIYTNTKRKPMILPIIIEV

Sequences:

>Translated_557_residues
MKSLKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDVTYLQENKEKIRGLVVTHGHEDHIG
GIPYFLKQLNVPIYATKLTLGLIEIKLKEHNLQNDTELIVIHSESEIDFGSIKTTFFKTNHSIPDCLGIAFHTPEGTVVH
TGDFKFDLTPVNNQQPDIHKMAKIGSEGVLALLSESTNAERPGFTPSERSVGERVEEIFMKANRKVIISTFASNVNRVQQ
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLNIPDGMLIEASEVNRLDPKQAAILCTGSQGEPMAALARLASGNYRQVD
VLPEDTVIIAATPIPGNERNVSRIIDNLFALGAKVIYGSGSSTGVHVSGHAYQEELKLMLALMKPKYFIPIHGEFRMLHH
HSLLAESIGVKKENIFIVRNGDVVDISNEVAIQSRKIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKADG
DIISGPDIISRGFVYVRDSEEFLKELNKLAVITINDLKKEKVNSWGILKREVREALGKYIYTNTKRKPMILPIIIEV
>Mature_557_residues
MKSLKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDVTYLQENKEKIRGLVVTHGHEDHIG
GIPYFLKQLNVPIYATKLTLGLIEIKLKEHNLQNDTELIVIHSESEIDFGSIKTTFFKTNHSIPDCLGIAFHTPEGTVVH
TGDFKFDLTPVNNQQPDIHKMAKIGSEGVLALLSESTNAERPGFTPSERSVGERVEEIFMKANRKVIISTFASNVNRVQQ
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLNIPDGMLIEASEVNRLDPKQAAILCTGSQGEPMAALARLASGNYRQVD
VLPEDTVIIAATPIPGNERNVSRIIDNLFALGAKVIYGSGSSTGVHVSGHAYQEELKLMLALMKPKYFIPIHGEFRMLHH
HSLLAESIGVKKENIFIVRNGDVVDISNEVAIQSRKIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKADG
DIISGPDIISRGFVYVRDSEEFLKELNKLAVITINDLKKEKVNSWGILKREVREALGKYIYTNTKRKPMILPIIIEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61563; Mature: 61563

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDVTYLQ
CCCCCCCCEEEEECCHHHHCCCEEEEEECCCEEEEECCCCCCCCCCEEEEEEECCHHHHH
ENKEKIRGLVVTHGHEDHIGGIPYFLKQLNVPIYATKLTLGLIEIKLKEHNLQNDTELIV
CCHHHHCEEEEECCCCCCCCCHHHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCCCEEEE
IHSESEIDFGSIKTTFFKTNHSIPDCLGIAFHTPEGTVVHTGDFKFDLTPVNNQQPDIHK
EECCCCCCCCCEEEEEEECCCCCCHHEEEEEECCCCCEEECCCEEEEEEECCCCCCCHHH
MAKIGSEGVLALLSESTNAERPGFTPSERSVGERVEEIFMKANRKVIISTFASNVNRVQQ
HHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHH
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLNIPDGMLIEASEVNRLDPKQAAILCTGS
HHHHHHHCCCEEEEHHHHHHHHHHHHHHCCEEECCCCCEEECCCCCCCCCCCEEEEEECC
QGEPMAALARLASGNYRQVDVLPEDTVIIAATPIPGNERNVSRIIDNLFALGAKVIYGSG
CCCHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECC
SSTGVHVSGHAYQEELKLMLALMKPKYFIPIHGEFRMLHHHSLLAESIGVKKENIFIVRN
CCCCEEEECHHHHHHHHHHHHHHCCCEEEEECCCEEEEHHHHHHHHHHCCCCCCEEEEEC
GDVVDISNEVAIQSRKIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKADG
CCEEECCCCEEEEEEEEEECCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
DIISGPDIISRGFVYVRDSEEFLKELNKLAVITINDLKKEKVNSWGILKREVREALGKYI
CEECCCCHHHCCEEEEECCHHHHHHHCCEEEEEECCCCHHHCCCCCHHHHHHHHHHHHHE
YTNTKRKPMILPIIIEV
EECCCCCCEEEEEEEEC
>Mature Secondary Structure
MKSLKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDVTYLQ
CCCCCCCCEEEEECCHHHHCCCEEEEEECCCEEEEECCCCCCCCCCEEEEEEECCHHHHH
ENKEKIRGLVVTHGHEDHIGGIPYFLKQLNVPIYATKLTLGLIEIKLKEHNLQNDTELIV
CCHHHHCEEEEECCCCCCCCCHHHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCCCEEEE
IHSESEIDFGSIKTTFFKTNHSIPDCLGIAFHTPEGTVVHTGDFKFDLTPVNNQQPDIHK
EECCCCCCCCCEEEEEEECCCCCCHHEEEEEECCCCCEEECCCEEEEEEECCCCCCCHHH
MAKIGSEGVLALLSESTNAERPGFTPSERSVGERVEEIFMKANRKVIISTFASNVNRVQQ
HHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHH
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLNIPDGMLIEASEVNRLDPKQAAILCTGS
HHHHHHHCCCEEEEHHHHHHHHHHHHHHCCEEECCCCCEEECCCCCCCCCCCEEEEEECC
QGEPMAALARLASGNYRQVDVLPEDTVIIAATPIPGNERNVSRIIDNLFALGAKVIYGSG
CCCHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECC
SSTGVHVSGHAYQEELKLMLALMKPKYFIPIHGEFRMLHHHSLLAESIGVKKENIFIVRN
CCCCEEEECHHHHHHHHHHHHHHCCCEEEEECCCEEEEHHHHHHHHHHCCCCCCEEEEEC
GDVVDISNEVAIQSRKIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKADG
CCEEECCCCEEEEEEEEEECCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
DIISGPDIISRGFVYVRDSEEFLKELNKLAVITINDLKKEKVNSWGILKREVREALGKYI
CEECCCCHHHCCEEEEECCHHHHHHHCCEEEEEECCCCHHHCCCCCHHHHHHHHHHHHHE
YTNTKRKPMILPIIIEV
EECCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]