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The map label for this gene is asnO [H]

Identifier: 52143657

GI number: 52143657

Start: 1657375

End: 1659198

Strand: Reverse

Name: asnO [H]

Synonym: BCZK1577

Alternate gene names: 52143657

Gene position: 1659198-1657375 (Counterclockwise)

Preceding gene: 52143654

Following gene: 52143658

Centisome position: 31.3

GC content: 36.4

Gene sequence:

>1824_bases
ATGTGTGGAATTACAGGTTGGGTAAATTGGAATAAGGACCTTTCTAATGAACATGTTATTTTAAAAAAGATGGCAAATCG
TATTCAGCATCGCGGTCCTGATGCTGAAGGATTTTGGTTTTCACCTCGTGCGGCCTTTGCACACCGTCGTTTAATTGTAA
TTGATCCAGAAGGCGGGGCACAGCCGAAGACATTTCGTGCTGGTGATTATACGTATGCTCTTACTTATAATGGAGAAATT
TATAATTTCCGTGAGCTGAGGGAACATCTTCAAAAATGTGGTCATACATTTGAAACGCATTCAGATACAGAAGTGTTACT
ACATGCTTATTTAGAATGGAAAGAAGACTGCGTGCAACATTTAAACGGCATTTTCGCTTTCGCCTTATGGGATGATCAGA
AGCAACGACTGTTTTTAGCACGTGATCATTTAGGTGTAAAACCGCTCTTTTATACAGAAAGAAATGACAGTATTATTTTC
GGATCTGAAATAAAAGCATTATTAGCTCATCCATCTGTTCCTGCTGAAATTGATGCGAATGGCATAAATGAAATATTTGG
ATTAGGGCTATTTCGAACTCCAGGATGTGGCGTCTTTAAACATATTGAAGAAGTGCGTGCCGGACATTCTATAACATTTA
CACGCGATAAAAAAGTAGTTACAAAGTATTGGAATTTAGAAAGTAAGGTCCATACAGATTCTATAGAAGATACATCATCA
CATATTTTATCTATTTTACAAGATACAGTAAAAAGACAATTAATTGCCGACGTCCCTCTCGTTTGCATGTTATCAGGAGG
GTTAGATTCTAGCGGTATTACTGCACTAGCAGGGAAAGAATTTGCTGCTGAAAATAAAACACTTCATACGTATTCCGTTG
ACTTTGTAAATAGCGCAAAAGATTTCGAGCTGACATTTGCTCGCACTGGTTTAGACGCTCCTTGGGTAAAACGCGTTTCT
GAACATGTAGGGACATCTCATCATGATATTATTGTGAATGCTGAAGAATTAGCAAATCGCTTATTCGTTCCCCTCCGTGC
AAAAGATTTACCGAGTGCTGGTGAAATGGAAACTTCACTATATTTACTATTTTGCGAAATGAAAAAAGATGCGACCGTAG
CTTTATCTGGCGAATCAGCTGATGAAGTATTCGGTGGATACCCTTGGTTTCATCAAGAAGAATTACTATATGTAGATAAG
TTTCCTTGGCTAACAAATTGGAAGAATACATCTCCTCTTCTACTACATGAAGTAAGTAACCAATGTAATCTAGAAAACTA
TATTGATACACGCTTCCAAAAAGCGATACTTGAAGTACCTACTCTTGAAGGCGAAAGTAAAAAATCCGCGAAACAACGTC
AAATGTTTTATTTATTTTTAACGAGATTTCTTCCGTTCTTACTTGACCGTAAAGACCGTATGAGTATGGCTGTAGGATTT
GAAGTTCGTGTACCGTTTTGCGATTATCGATTAGTTGAATATTTATGGAACGTTCCTTTCAACATAAAAAGCATTGATAA
TATTGAAAAAGGTATTTTACGTAGAGCACTGAAACCTGCTTTACCTGACGATGTACGCAATAGAAGAAAAAGTGCTTATC
CAACTTCACAAGACCCACATTATTTACAAACAATCCGGAATTTAACACTTGACATGTGTAGTAATAAAAACAACCCTATT
TTCTCACTTATTAATCATTCCACATTACTTGCCATTGCCAATCAGAGTAATAAAGAAATTAACAATTTCGAAGCTAGAAG
TGCTATAGAATACATGCTACAAGTAAACGAATGGTTAAAAACATATCATATTCATATTGCTTAA

Upstream 100 bases:

>100_bases
AATTTGATTCATGTAGTACACTATTTATATAGTCATTCTTTAACTTAGTGTACATTTTATTTCGTTAACCTACTATTTAA
AATTAGGAGATGAGATGTGT

Downstream 100 bases:

>100_bases
CAAAAAGGAGTTCAAGGATAAAAACCTTGAACTCCTTTTATATTAATTAGCACATCGTTTATTTTCAGTGTCAATGGACC
ATCCAATAATTGTGGCACAA

Product: asparagine synthetase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 607; Mature: 607

Protein sequence:

>607_residues
MCGITGWVNWNKDLSNEHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGAQPKTFRAGDYTYALTYNGEI
YNFRELREHLQKCGHTFETHSDTEVLLHAYLEWKEDCVQHLNGIFAFALWDDQKQRLFLARDHLGVKPLFYTERNDSIIF
GSEIKALLAHPSVPAEIDANGINEIFGLGLFRTPGCGVFKHIEEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAKDFELTFARTGLDAPWVKRVS
EHVGTSHHDIIVNAEELANRLFVPLRAKDLPSAGEMETSLYLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDK
FPWLTNWKNTSPLLLHEVSNQCNLENYIDTRFQKAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALKPALPDDVRNRRKSAYPTSQDPHYLQTIRNLTLDMCSNKNNPI
FSLINHSTLLAIANQSNKEINNFEARSAIEYMLQVNEWLKTYHIHIA

Sequences:

>Translated_607_residues
MCGITGWVNWNKDLSNEHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGAQPKTFRAGDYTYALTYNGEI
YNFRELREHLQKCGHTFETHSDTEVLLHAYLEWKEDCVQHLNGIFAFALWDDQKQRLFLARDHLGVKPLFYTERNDSIIF
GSEIKALLAHPSVPAEIDANGINEIFGLGLFRTPGCGVFKHIEEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAKDFELTFARTGLDAPWVKRVS
EHVGTSHHDIIVNAEELANRLFVPLRAKDLPSAGEMETSLYLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDK
FPWLTNWKNTSPLLLHEVSNQCNLENYIDTRFQKAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALKPALPDDVRNRRKSAYPTSQDPHYLQTIRNLTLDMCSNKNNPI
FSLINHSTLLAIANQSNKEINNFEARSAIEYMLQVNEWLKTYHIHIA
>Mature_607_residues
MCGITGWVNWNKDLSNEHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGAQPKTFRAGDYTYALTYNGEI
YNFRELREHLQKCGHTFETHSDTEVLLHAYLEWKEDCVQHLNGIFAFALWDDQKQRLFLARDHLGVKPLFYTERNDSIIF
GSEIKALLAHPSVPAEIDANGINEIFGLGLFRTPGCGVFKHIEEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAKDFELTFARTGLDAPWVKRVS
EHVGTSHHDIIVNAEELANRLFVPLRAKDLPSAGEMETSLYLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDK
FPWLTNWKNTSPLLLHEVSNQCNLENYIDTRFQKAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALKPALPDDVRNRRKSAYPTSQDPHYLQTIRNLTLDMCSNKNNPI
FSLINHSTLLAIANQSNKEINNFEARSAIEYMLQVNEWLKTYHIHIA

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=554, Percent_Identity=28.158844765343, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI168229252, Length=554, Percent_Identity=28.158844765343, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI168229250, Length=554, Percent_Identity=28.158844765343, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI296010848, Length=528, Percent_Identity=28.030303030303, Blast_Score=163, Evalue=5e-40,
Organism=Homo sapiens, GI296010852, Length=454, Percent_Identity=26.2114537444934, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI296010850, Length=454, Percent_Identity=26.2114537444934, Blast_Score=123, Evalue=5e-28,
Organism=Escherichia coli, GI1786889, Length=578, Percent_Identity=27.3356401384083, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI25147557, Length=533, Percent_Identity=27.5797373358349, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71993933, Length=533, Percent_Identity=27.5797373358349, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25147560, Length=321, Percent_Identity=26.791277258567, Blast_Score=109, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321563, Length=431, Percent_Identity=28.538283062645, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6325403, Length=420, Percent_Identity=29.5238095238095, Blast_Score=140, Evalue=6e-34,
Organism=Drosophila melanogaster, GI45553209, Length=378, Percent_Identity=28.8359788359788, Blast_Score=133, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 69607; Mature: 69607

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGWVNWNKDLSNEHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCHHHHHCCEEEEECCCCCC
QPKTFRAGDYTYALTYNGEIYNFRELREHLQKCGHTFETHSDTEVLLHAYLEWKEDCVQH
CCCEEECCCEEEEEEECCCEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
LNGIFAFALWDDQKQRLFLARDHLGVKPLFYTERNDSIIFGSEIKALLAHPSVPAEIDAN
HCCEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCCEEEHHHHHHHHCCCCCCCCCCCC
GINEIFGLGLFRTPGCGVFKHIEEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
CHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCCCHHHHH
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAK
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCHHHCCCCEEEEEEEEECCCCC
DFELTFARTGLDAPWVKRVSEHVGTSHHDIIVNAEELANRLFVPLRAKDLPSAGEMETSL
CEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHEEEEEECCCCCCCCCCCCEE
YLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDKFPWLTNWKNTSPLLLHEVSN
EEEEEECCCCCEEEECCCCHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEECCC
QCNLENYIDTRFQKAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCEEEEEE
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALKPALPDDVRNRRKSAYPTSQDPH
EEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCH
YLQTIRNLTLDMCSNKNNPIFSLINHSTLLAIANQSNKEINNFEARSAIEYMLQVNEWLK
HHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
TYHIHIA
HEEEEEC
>Mature Secondary Structure
MCGITGWVNWNKDLSNEHVILKKMANRIQHRGPDAEGFWFSPRAAFAHRRLIVIDPEGGA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCHHHHHCCEEEEECCCCCC
QPKTFRAGDYTYALTYNGEIYNFRELREHLQKCGHTFETHSDTEVLLHAYLEWKEDCVQH
CCCEEECCCEEEEEEECCCEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
LNGIFAFALWDDQKQRLFLARDHLGVKPLFYTERNDSIIFGSEIKALLAHPSVPAEIDAN
HCCEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCCEEEHHHHHHHHCCCCCCCCCCCC
GINEIFGLGLFRTPGCGVFKHIEEVRAGHSITFTRDKKVVTKYWNLESKVHTDSIEDTSS
CHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCCCHHHHH
HILSILQDTVKRQLIADVPLVCMLSGGLDSSGITALAGKEFAAENKTLHTYSVDFVNSAK
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCHHHCCCCEEEEEEEEECCCCC
DFELTFARTGLDAPWVKRVSEHVGTSHHDIIVNAEELANRLFVPLRAKDLPSAGEMETSL
CEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHEEEEEECCCCCCCCCCCCEE
YLLFCEMKKDATVALSGESADEVFGGYPWFHQEELLYVDKFPWLTNWKNTSPLLLHEVSN
EEEEEECCCCCEEEECCCCHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEECCC
QCNLENYIDTRFQKAILEVPTLEGESKKSAKQRQMFYLFLTRFLPFLLDRKDRMSMAVGF
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCEEEEEE
EVRVPFCDYRLVEYLWNVPFNIKSIDNIEKGILRRALKPALPDDVRNRRKSAYPTSQDPH
EEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCH
YLQTIRNLTLDMCSNKNNPIFSLINHSTLLAIANQSNKEINNFEARSAIEYMLQVNEWLK
HHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
TYHIHIA
HEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]