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The map label for this gene is puuR [H]

Identifier: 52143637

GI number: 52143637

Start: 1679630

End: 1680175

Strand: Reverse

Name: puuR [H]

Synonym: BCZK1599

Alternate gene names: 52143637

Gene position: 1680175-1679630 (Counterclockwise)

Preceding gene: 52143632

Following gene: 52143642

Centisome position: 31.7

GC content: 31.68

Gene sequence:

>546_bases
ATGGAAAATATAGATATTGGTAAAAAAATTGAAAAACAAAGAAAAGAAAAAGGTTTAACGAGTAAAGAACTTGCAAAGAT
GGCTGATATTACACCATCTATGTTAAGTCAAATTGAGCGTGGATCTGCTAACCCTTCTATCCAAACATTAAAAGTACTCG
CTAAGGCTCTTGATGTTCCAACATTTAGCTTTTTACTTGAGGATACAAATACAGACGATTTAATTGTACGTTCTCATAAA
CGCAAAAAAATGATTATCGATAATTTATCATATGAAATGCTATCACCTAATTTCACAGGAAATTTAGCAACAGCAATTAT
GACCGTCCCGCCGAATACAGCTTCATCAAAAAATGTACTAGAACATAAAGGGGAAGAATTAGCATTTGTATTAGACGGGA
AAATTACATTACATTTAAATGAAGAAGAATACATATTAGAAACGGGTGATAGCGTAAAAATACCCGCTTATTTAAAACAT
AAATGGGTAAATCAATTCGAGCAAAATGCCATTGTTTTATTTTCTGTTACTCCGCCGATTTTTTAA

Upstream 100 bases:

>100_bases
AAATAAATTTTAATATAGTTTAACATAAATTATCCACTTTAGTTAATTTTAATTTAATTTATTTAAATATAACTTAATAA
TTAGTAAGGAGATTTTCAAT

Downstream 100 bases:

>100_bases
TAGCTGTAATAAAAATAGCCACGTGCGGATTCCTCCGCACATGGCTATTTTTATCTTTTCGGTACGAACAAAATCCCTAA
ACTCATAACACAAAAACTGA

Product: MerR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MENIDIGKKIEKQRKEKGLTSKELAKMADITPSMLSQIERGSANPSIQTLKVLAKALDVPTFSFLLEDTNTDDLIVRSHK
RKKMIIDNLSYEMLSPNFTGNLATAIMTVPPNTASSKNVLEHKGEELAFVLDGKITLHLNEEEYILETGDSVKIPAYLKH
KWVNQFEQNAIVLFSVTPPIF

Sequences:

>Translated_181_residues
MENIDIGKKIEKQRKEKGLTSKELAKMADITPSMLSQIERGSANPSIQTLKVLAKALDVPTFSFLLEDTNTDDLIVRSHK
RKKMIIDNLSYEMLSPNFTGNLATAIMTVPPNTASSKNVLEHKGEELAFVLDGKITLHLNEEEYILETGDSVKIPAYLKH
KWVNQFEQNAIVLFSVTPPIF
>Mature_181_residues
MENIDIGKKIEKQRKEKGLTSKELAKMADITPSMLSQIERGSANPSIQTLKVLAKALDVPTFSFLLEDTNTDDLIVRSHK
RKKMIIDNLSYEMLSPNFTGNLATAIMTVPPNTASSKNVLEHKGEELAFVLDGKITLHLNEEEYILETGDSVKIPAYLKH
KWVNQFEQNAIVLFSVTPPIF

Specific function: Represses puuD [H]

COG id: COG1396

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=185, Percent_Identity=27.5675675675676, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1787704, Length=173, Percent_Identity=26.5895953757225, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 20314; Mature: 20314

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENIDIGKKIEKQRKEKGLTSKELAKMADITPSMLSQIERGSANPSIQTLKVLAKALDVP
CCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
TFSFLLEDTNTDDLIVRSHKRKKMIIDNLSYEMLSPNFTGNLATAIMTVPPNTASSKNVL
CEEEEEECCCCCCEEEECCCCCHHHHHCCCHHHCCCCCCCCEEEEEEEECCCCCCCCHHH
EHKGEELAFVLDGKITLHLNEEEYILETGDSVKIPAYLKHKWVNQFEQNAIVLFSVTPPI
HCCCCEEEEEECCEEEEEECCCEEEEECCCCEECCHHHHHHHHHHHHCCEEEEEECCCCC
F
C
>Mature Secondary Structure
MENIDIGKKIEKQRKEKGLTSKELAKMADITPSMLSQIERGSANPSIQTLKVLAKALDVP
CCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
TFSFLLEDTNTDDLIVRSHKRKKMIIDNLSYEMLSPNFTGNLATAIMTVPPNTASSKNVL
CEEEEEECCCCCCEEEECCCCCHHHHHCCCHHHCCCCCCCCEEEEEEEECCCCCCCCHHH
EHKGEELAFVLDGKITLHLNEEEYILETGDSVKIPAYLKHKWVNQFEQNAIVLFSVTPPI
HCCCCEEEEEECCEEEEEECCCEEEEECCCCEECCHHHHHHHHHHHHCCEEEEEECCCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]