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The map label for this gene is oppA [H]

Identifier: 52143396

GI number: 52143396

Start: 1972623

End: 1974284

Strand: Direct

Name: oppA [H]

Synonym: BCZK1846

Alternate gene names: 52143396

Gene position: 1972623-1974284 (Clockwise)

Preceding gene: 52143395

Following gene: 52143387

Centisome position: 37.21

GC content: 33.39

Gene sequence:

>1662_bases
ATGAAGAGAAAGGTAACAACGATTGCTGCAGTTGCATTATCGACTAGTATATTAGTTGCAGGTTGCGGAAATGGGGAGAA
GGCATCTACAACGAAGAAAGAAGCAGGTAAGGAGGTAGCAGATAAGCAAGTATTAAACTTACTAGAAACAGCAGAAATTC
CTTCAATGGATACGTCAAAATCAACAGATTCTGTATCATTCCGTGCCTTTGTAAATGCAATGGAAGGATTATATCGTTTA
GATAAGGACAATAAACCAACTCCAGGTATGGCAAAAGACGTGAAAATTAGTGAAGATAAAAAGACATATACATTTGAATT
ACGTGATGCGAAATGGTCAAATGGTGATCCAGTTCGAGCACAAGATTTTGTATATGCATGGCAACGTACATTAGATAAAG
CAATGGCAGCTGAGTATGCATTTATTTTATTTGATGTGAAAAATGCACAAAAAGTAAATAAAGGAGAGTTACCGTTAGAT
CAATTAGGAGTGAAAGCGAAGGATGATAAAACGTTAGTAGTAGAATTAGAACAACCAGCACCATATTTCCTTGAGCTTAC
TTCATTCCCAACATTTTTCCCTTTAAATGAAAAATATGTGAAAGAACAAGGAGATAAATATGGATTAGAAGCAGATAAAT
TGCTATATAATGGACCATTTACGATGAGTGAATGGAAACATGAAAGAAGTTATCAGTTAAAGAAAAATCCTAATTATTGG
GATAAAGATACTGTGAAGTTAGAAGAAATTAATGTGAATATTGTGAAGGAAACAAGCACAGGAGTAAATTTATATGATAG
TGATCAAGCAGATCGGGTTATTTTAACTTCTGAATTTGTTGATAAATATAAAAAGAATAAGCCAGATGAATTTAAAACGA
AGTTAGATCCGCGTATGTATTTCTTGCGCTTTAACCAAAAAAATGCAACGTTTAAAAACCAAAAGGTTCGTGAAGCAATT
GATCTAGCATATGATAAAAAAGGAATTGCAAATGTTATTTTAAATGACGGATCATTGCCAGCATATTTCTTAGTACCTGA
AAAATTTACGACAGGACCTGATGGGAAAGACTTCCGTACTGTAAATAAAAATTTCCGTGACAGTAAAGATAATGCAGCAA
AAGCAAAAAAATTATGGGAAGAAGCGAAGAAAGAACTTGGCCAAGATACAATTACAGTAGAATTACTAAATGATGATAAC
GGTAGTCGTAAAAAAGTAGGAGACTTTATTAAAGAAGAGTTAGAGAAAAACTTACCTGGTATTAAAGTGAATTTAAAACA
ACAGCCATATAAGCAGAAGTTGGATTTAGAAAAGAAATTTGAATATGAATTCTCTTTATCTGCATGGAGTCCAGATTATC
CAGATCCAATGACTTATATTGATATGTTTGTTACTGGAAGCTCATTTAATGAAATGGATTATTCTAATCCGAAGTATGAT
GAGTTAGTAGCAAAATCAAAAGGAGAGCTATTAAAAGATGATAAAGCACGCTGGAATGCACTTGCAGAAGCGGAAAAAAT
CTTAATTGGTCAAGATGCTGCCATTTCTCCTACGTACCAACAAAGTACCGCATACTTAGAAAAACCATACGTAAAAGATA
TTGCAAATCATACGTTTGGTGGGGACTTCAGTTATAAATGGGCATATATTACAAAAAAATAG

Upstream 100 bases:

>100_bases
TGTATATACGAAAATAATATTTGAAATAATCTGAATCTTTTGTATAATTATATGTATTATACTGGCTTTCATTTATACAT
ATAGATTGGGGGAAGGAAGA

Downstream 100 bases:

>100_bases
TACAATAAAAGATGAGTGAATTTCCGCTCATCTTTTATTTGTATAGGAGGAATTAGAGTTGTTAAAAGGAATCAATCATC
TTTGTTTTTCAGTATCTAAT

Product: oligopeptide ABC transporter, substrate-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MKRKVTTIAAVALSTSILVAGCGNGEKASTTKKEAGKEVADKQVLNLLETAEIPSMDTSKSTDSVSFRAFVNAMEGLYRL
DKDNKPTPGMAKDVKISEDKKTYTFELRDAKWSNGDPVRAQDFVYAWQRTLDKAMAAEYAFILFDVKNAQKVNKGELPLD
QLGVKAKDDKTLVVELEQPAPYFLELTSFPTFFPLNEKYVKEQGDKYGLEADKLLYNGPFTMSEWKHERSYQLKKNPNYW
DKDTVKLEEINVNIVKETSTGVNLYDSDQADRVILTSEFVDKYKKNKPDEFKTKLDPRMYFLRFNQKNATFKNQKVREAI
DLAYDKKGIANVILNDGSLPAYFLVPEKFTTGPDGKDFRTVNKNFRDSKDNAAKAKKLWEEAKKELGQDTITVELLNDDN
GSRKKVGDFIKEELEKNLPGIKVNLKQQPYKQKLDLEKKFEYEFSLSAWSPDYPDPMTYIDMFVTGSSFNEMDYSNPKYD
ELVAKSKGELLKDDKARWNALAEAEKILIGQDAAISPTYQQSTAYLEKPYVKDIANHTFGGDFSYKWAYITKK

Sequences:

>Translated_553_residues
MKRKVTTIAAVALSTSILVAGCGNGEKASTTKKEAGKEVADKQVLNLLETAEIPSMDTSKSTDSVSFRAFVNAMEGLYRL
DKDNKPTPGMAKDVKISEDKKTYTFELRDAKWSNGDPVRAQDFVYAWQRTLDKAMAAEYAFILFDVKNAQKVNKGELPLD
QLGVKAKDDKTLVVELEQPAPYFLELTSFPTFFPLNEKYVKEQGDKYGLEADKLLYNGPFTMSEWKHERSYQLKKNPNYW
DKDTVKLEEINVNIVKETSTGVNLYDSDQADRVILTSEFVDKYKKNKPDEFKTKLDPRMYFLRFNQKNATFKNQKVREAI
DLAYDKKGIANVILNDGSLPAYFLVPEKFTTGPDGKDFRTVNKNFRDSKDNAAKAKKLWEEAKKELGQDTITVELLNDDN
GSRKKVGDFIKEELEKNLPGIKVNLKQQPYKQKLDLEKKFEYEFSLSAWSPDYPDPMTYIDMFVTGSSFNEMDYSNPKYD
ELVAKSKGELLKDDKARWNALAEAEKILIGQDAAISPTYQQSTAYLEKPYVKDIANHTFGGDFSYKWAYITKK
>Mature_553_residues
MKRKVTTIAAVALSTSILVAGCGNGEKASTTKKEAGKEVADKQVLNLLETAEIPSMDTSKSTDSVSFRAFVNAMEGLYRL
DKDNKPTPGMAKDVKISEDKKTYTFELRDAKWSNGDPVRAQDFVYAWQRTLDKAMAAEYAFILFDVKNAQKVNKGELPLD
QLGVKAKDDKTLVVELEQPAPYFLELTSFPTFFPLNEKYVKEQGDKYGLEADKLLYNGPFTMSEWKHERSYQLKKNPNYW
DKDTVKLEEINVNIVKETSTGVNLYDSDQADRVILTSEFVDKYKKNKPDEFKTKLDPRMYFLRFNQKNATFKNQKVREAI
DLAYDKKGIANVILNDGSLPAYFLVPEKFTTGPDGKDFRTVNKNFRDSKDNAAKAKKLWEEAKKELGQDTITVELLNDDN
GSRKKVGDFIKEELEKNLPGIKVNLKQQPYKQKLDLEKKFEYEFSLSAWSPDYPDPMTYIDMFVTGSSFNEMDYSNPKYD
ELVAKSKGELLKDDKARWNALAEAEKILIGQDAAISPTYQQSTAYLEKPYVKDIANHTFGGDFSYKWAYITKK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=534, Percent_Identity=30.3370786516854, Blast_Score=226, Evalue=4e-60,
Organism=Escherichia coli, GI1789397, Length=510, Percent_Identity=27.4509803921569, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI87081878, Length=542, Percent_Identity=28.9667896678967, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1787762, Length=539, Percent_Identity=24.30426716141, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787052, Length=475, Percent_Identity=21.8947368421053, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 63146; Mature: 63146

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKVTTIAAVALSTSILVAGCGNGEKASTTKKEAGKEVADKQVLNLLETAEIPSMDTSK
CCCCHHHHHHHHHHCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
STDSVSFRAFVNAMEGLYRLDKDNKPTPGMAKDVKISEDKKTYTFELRDAKWSNGDPVRA
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCCCCCCCCCH
QDFVYAWQRTLDKAMAAEYAFILFDVKNAQKVNKGELPLDQLGVKAKDDKTLVVELEQPA
HHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCC
PYFLELTSFPTFFPLNEKYVKEQGDKYGLEADKLLYNGPFTMSEWKHERSYQLKKNPNYW
CEEEEECCCCCCCCCCHHHHHHCCCCCCCCHHHEEECCCCCHHHHCCCCCEEECCCCCCC
DKDTVKLEEINVNIVKETSTGVNLYDSDQADRVILTSEFVDKYKKNKPDEFKTKLDPRMY
CCCCEEEEEEEEEEEEECCCCCEEECCCCCCEEEEEHHHHHHHHCCCCHHHHHCCCCEEE
FLRFNQKNATFKNQKVREAIDLAYDKKGIANVILNDGSLPAYFLVPEKFTTGPDGKDFRT
EEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCHHH
VNKNFRDSKDNAAKAKKLWEEAKKELGQDTITVELLNDDNGSRKKVGDFIKEELEKNLPG
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCC
IKVNLKQQPYKQKLDLEKKFEYEFSLSAWSPDYPDPMTYIDMFVTGSSFNEMDYSNPKYD
EEEECCCCCCHHHCCCCHHCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHH
ELVAKSKGELLKDDKARWNALAEAEKILIGQDAAISPTYQQSTAYLEKPYVKDIANHTFG
HHHHHCCCCHHCCCHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHCCCHHHHHHCCCCC
GDFSYKWAYITKK
CCCEEEEEEEECC
>Mature Secondary Structure
MKRKVTTIAAVALSTSILVAGCGNGEKASTTKKEAGKEVADKQVLNLLETAEIPSMDTSK
CCCCHHHHHHHHHHCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
STDSVSFRAFVNAMEGLYRLDKDNKPTPGMAKDVKISEDKKTYTFELRDAKWSNGDPVRA
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCCCCCCCCCH
QDFVYAWQRTLDKAMAAEYAFILFDVKNAQKVNKGELPLDQLGVKAKDDKTLVVELEQPA
HHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCC
PYFLELTSFPTFFPLNEKYVKEQGDKYGLEADKLLYNGPFTMSEWKHERSYQLKKNPNYW
CEEEEECCCCCCCCCCHHHHHHCCCCCCCCHHHEEECCCCCHHHHCCCCCEEECCCCCCC
DKDTVKLEEINVNIVKETSTGVNLYDSDQADRVILTSEFVDKYKKNKPDEFKTKLDPRMY
CCCCEEEEEEEEEEEEECCCCCEEECCCCCCEEEEEHHHHHHHHCCCCHHHHHCCCCEEE
FLRFNQKNATFKNQKVREAIDLAYDKKGIANVILNDGSLPAYFLVPEKFTTGPDGKDFRT
EEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCHHH
VNKNFRDSKDNAAKAKKLWEEAKKELGQDTITVELLNDDNGSRKKVGDFIKEELEKNLPG
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCC
IKVNLKQQPYKQKLDLEKKFEYEFSLSAWSPDYPDPMTYIDMFVTGSSFNEMDYSNPKYD
EEEECCCCCCHHHCCCCHHCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHH
ELVAKSKGELLKDDKARWNALAEAEKILIGQDAAISPTYQQSTAYLEKPYVKDIANHTFG
HHHHHCCCCHHCCCHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHCCCHHHHHHCCCCC
GDFSYKWAYITKK
CCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]