Click here to switch to the map view.

The map label for this gene is aph [H]

Identifier: 52143338

GI number: 52143338

Start: 2017532

End: 2018413

Strand: Direct

Name: aph [H]

Synonym: BCZK1900

Alternate gene names: 52143338

Gene position: 2017532-2018413 (Clockwise)

Preceding gene: 52143337

Following gene: 52143333

Centisome position: 38.06

GC content: 32.09

Gene sequence:

>882_bases
ATGAGAAAAGAAAATGAGTATGTTACGTATTTGCAAAGGTTGTATCCTGATTTACAAATAAATTCCGTTTATATAAATGA
AATAGGTCAAAATAATGATGTGTTAATTGTAAATGATAATATAGTATTCCGATTTCCGAAATATGAAAAAGGTATTCAGA
AACTTCGAATAGAAACACAGTTGCTAGAGAAAATAAGACCCTTCATTACGCTTCAAATTCCCAATCCAAGTTATCAAGGG
TTTCAAAATGAAGTACCAGGGAAAGTATTTGCAGGTTATGAGATGATTGAAGGAGACCCATTTTGGAAAAATGTTTTTAC
GGAAATAAATGATGAAAAGCGATTACAGAAACTTGCGTGTACATTGGCGAGATTTTTAAAAGAGTTGCATGAAATTCCGT
TATCTACATTCGAAGGTATTATGCAATGTGATAGTACTGATATGTATTCAGAAATAAATAGTTTATATAGCCAATTAAAA
GAACATGTTTATCCATTCATGAGAAATGTAGCGAGAAAAGAGGTCTCAACATCGTTTGAGTTATATTTAAATGAAAGTAG
CCATTTTAACTTTACTCCTAGTCTTGTTCATGGGGACTTTGGCATGACAAACATTTTATATAGTGCAACAAAAAAGAATA
TTTCAGGGGTCATAGATTTTGGCGGGGCGAGCATTGGAGATCCAGCTTATGATTTTGCTGGTATATTAGCTAGTTATGGA
GAAGAGTTTTTACAACTATTTGAAGCCTATTATCCTAATTTAGAAGCAGTAAAGGAGCGAATGTATTTTTATAAGAGTAC
ATTTGCGCTTCAAGAAGCATTATTTGGCGTACTAAATAATGATAAGAAAGCTTTTGAAGCTGGAATAGCACAATATGTAT
AA

Upstream 100 bases:

>100_bases
AATTTTGCAAAAGAGATGTATGAACAGTTTGGTTTTGTTGAAAGGCAGGTAATTATGACTTTGAAGTGAGAATAATAGTT
TAAACGGGAGAAGTGAAAGC

Downstream 100 bases:

>100_bases
AAGCCGATTTTAAATCGGCTTTTAATTATCGATACATCGTTTACATCCCAATACCTTTAAACCCGAATCGTTTATAAATT
CGTCCAGCAGCTGGGTTCTT

Product: kanamycin kinase (aminoglycoside phosphotransferase, gentamicin resistance protein)

Products: NA

Alternate protein names: Viomycin kinase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MRKENEYVTYLQRLYPDLQINSVYINEIGQNNDVLIVNDNIVFRFPKYEKGIQKLRIETQLLEKIRPFITLQIPNPSYQG
FQNEVPGKVFAGYEMIEGDPFWKNVFTEINDEKRLQKLACTLARFLKELHEIPLSTFEGIMQCDSTDMYSEINSLYSQLK
EHVYPFMRNVARKEVSTSFELYLNESSHFNFTPSLVHGDFGMTNILYSATKKNISGVIDFGGASIGDPAYDFAGILASYG
EEFLQLFEAYYPNLEAVKERMYFYKSTFALQEALFGVLNNDKKAFEAGIAQYV

Sequences:

>Translated_293_residues
MRKENEYVTYLQRLYPDLQINSVYINEIGQNNDVLIVNDNIVFRFPKYEKGIQKLRIETQLLEKIRPFITLQIPNPSYQG
FQNEVPGKVFAGYEMIEGDPFWKNVFTEINDEKRLQKLACTLARFLKELHEIPLSTFEGIMQCDSTDMYSEINSLYSQLK
EHVYPFMRNVARKEVSTSFELYLNESSHFNFTPSLVHGDFGMTNILYSATKKNISGVIDFGGASIGDPAYDFAGILASYG
EEFLQLFEAYYPNLEAVKERMYFYKSTFALQEALFGVLNNDKKAFEAGIAQYV
>Mature_293_residues
MRKENEYVTYLQRLYPDLQINSVYINEIGQNNDVLIVNDNIVFRFPKYEKGIQKLRIETQLLEKIRPFITLQIPNPSYQG
FQNEVPGKVFAGYEMIEGDPFWKNVFTEINDEKRLQKLACTLARFLKELHEIPLSTFEGIMQCDSTDMYSEINSLYSQLK
EHVYPFMRNVARKEVSTSFELYLNESSHFNFTPSLVHGDFGMTNILYSATKKNISGVIDFGGASIGDPAYDFAGILASYG
EEFLQLFEAYYPNLEAVKERMYFYKSTFALQEALFGVLNNDKKAFEAGIAQYV

Specific function: The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation [H]

COG id: COG3173

COG function: function code R; Predicted aminoglycoside phosphotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aminoglycoside phosphotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009
- InterPro:   IPR012877 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.103 [H]

Molecular weight: Translated: 33883; Mature: 33883

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKENEYVTYLQRLYPDLQINSVYINEIGQNNDVLIVNDNIVFRFPKYEKGIQKLRIETQ
CCCCHHHHHHHHHHCCCCEECEEEEECCCCCCCEEEEECCEEEECCCHHHHHHHHHHHHH
LLEKIRPFITLQIPNPSYQGFQNEVPGKVFAGYEMIEGDPFWKNVFTEINDEKRLQKLAC
HHHHHCCEEEEEECCCCCCCCCCCCCCCEEECCEEECCCHHHHHHHHHCCHHHHHHHHHH
TLARFLKELHEIPLSTFEGIMQCDSTDMYSEINSLYSQLKEHVYPFMRNVARKEVSTSFE
HHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LYLNESSHFNFTPSLVHGDFGMTNILYSATKKNISGVIDFGGASIGDPAYDFAGILASYG
EEEECCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHH
EEFLQLFEAYYPNLEAVKERMYFYKSTFALQEALFGVLNNDKKAFEAGIAQYV
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRKENEYVTYLQRLYPDLQINSVYINEIGQNNDVLIVNDNIVFRFPKYEKGIQKLRIETQ
CCCCHHHHHHHHHHCCCCEECEEEEECCCCCCCEEEEECCEEEECCCHHHHHHHHHHHHH
LLEKIRPFITLQIPNPSYQGFQNEVPGKVFAGYEMIEGDPFWKNVFTEINDEKRLQKLAC
HHHHHCCEEEEEECCCCCCCCCCCCCCCEEECCEEECCCHHHHHHHHHCCHHHHHHHHHH
TLARFLKELHEIPLSTFEGIMQCDSTDMYSEINSLYSQLKEHVYPFMRNVARKEVSTSFE
HHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LYLNESSHFNFTPSLVHGDFGMTNILYSATKKNISGVIDFGGASIGDPAYDFAGILASYG
EEEECCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHH
EEFLQLFEAYYPNLEAVKERMYFYKSTFALQEALFGVLNNDKKAFEAGIAQYV
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987648 [H]