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The map label for this gene is narH [H]

Identifier: 52143304

GI number: 52143304

Start: 2046786

End: 2048261

Strand: Direct

Name: narH [H]

Synonym: BCZK1933

Alternate gene names: 52143304

Gene position: 2046786-2048261 (Clockwise)

Preceding gene: 52143305

Following gene: 52143303

Centisome position: 38.61

GC content: 38.48

Gene sequence:

>1476_bases
TTGAAGATTAAAGCACAAGTCGGAATGGTAATGAACCTAGATAAATGCATCGGCTGCCATACTTGTAGCGTAACATGCAA
AAACACCTGGACAAATCGTCCAGGTGCTGAATATATGTATTTCAATAACGTAGAAACAAAACCTGGTATTGGTTATCCGA
AGCAATGGGAAGATCAGGAGAAATATAAAGGCGGTTGGGAATTAAAAAATGGTGAAATTCAGCTGAAATCCGGTTCGAAA
ATGAAGCGCCTTATGAATATTTTCCACAATCCAGATCAACCAACAATTGATGATTACTTTGAACCGTGGAACTATGATTA
TGAAACGTTAACAAATAGTCCGCAGCGTAAGCATCAGCCAGTTGCTCGTCCGAAATCAGCAATTACAGGCGAATTTATCG
ACAAAATTGAATGGGGTCCAAACTGGGAAGATGATTTAGCTGGTGGACATATAACAGGATTACAAGATCCGAACGTAAAG
AAAATGGAAGAAGAAATTAAAACCGATTTTGAAAATGTCTTTATGATGTATTTACCACGCATATGCGAACATTGTATGAA
TCCATCTTGCGTATCGTCTTGTCCATCTGGTGCGATGTATAAACGGGAAGAAGATGGGATTGTTCTTGTAGATCAAAATG
CATGTCGTGCATGGAGATTCTGCGTATCGTCTTGCCCATATAAAAAAGTGTACTTTAACTGGCAAACGAATAAAGCGGAA
AAATGTACAATGTGTTTCCCACGTATTGAAGCTGGTATGCCAACAATTTGTTCGGAAACATGTGTAGGACGTATCAGGTA
TATTGGGGTAATGCTATATGACGCTGATAAAGTAAAAGAAGCGGCTTCTGTTGAGAATGAAAAAGATTTATATGAATCTC
AGCTTACTGTTTTCCTAGATCCAAATGATCCTGAAGTAGCAGCTGAAGCGAAAAAACAAGGCATTCCTGAAGAGTGGATT
AAAGCGGCACAACAGTCACCAATCTATAAAATGATTATCGATTGGAAAATTGCTCTACCACTTCACCCAGAGTATCGTAC
AATGCCAATGGTTTGGTATATCCCGCCACTTAGTCCAATTATGAATATGGTGGAAGGGAAAGGTAGTAACTGGCAAGCAG
AAGAAATTTTCCCTGCTATCGATAATATGCGTATTCCAATTCAATATTTAGCCAATCTACTTACTGCAGGAGATGAATCA
CACATTCGACTTACATTGAAAAAAATGGCTGTTATGCGAACGCATATGAGAGCACTGCAAATTAATAAAGAACCAAATGA
AACTGTATTAAAAGAACTTGGCTTAACGAAACAGGATGTAGAAGATATGTATCGTCTTCTTGCCATCGCAAAATATAAAG
ATCGTTTTGTTATTCCGACGTCTCACAGAGAACAAGTTGCAGATTTATACAGTGAACAAGGAAGCTGTGGTTTATCATTC
GCAGGTGGTCCAGGCTCTTGTATGACAATTTCGTAA

Upstream 100 bases:

>100_bases
GGATATGGTCAATTAAGTTATGGATTTAACTATTATGGTCCAACTGGGAACCAGCGCGATTTAAACGTAGTAATTCGCAA
ACTGAAGGAGGTAGATTGGC

Downstream 100 bases:

>100_bases
ATAAAGGGGGCAGAAAAATATGAAACAAAGTTTACAAACTACTTTTTCCTGTTCTTCATTCCTTCTCTCCTACCCGGAAC
TCGGGTGGAGGGAAGCTGTA

Product: respiratory nitrate reductase, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSK
MKRLMNIFHNPDQPTIDDYFEPWNYDYETLTNSPQRKHQPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVK
KMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYFNWQTNKAE
KCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKVKEAASVENEKDLYESQLTVFLDPNDPEVAAEAKKQGIPEEWI
KAAQQSPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLSPIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDES
HIRLTLKKMAVMRTHMRALQINKEPNETVLKELGLTKQDVEDMYRLLAIAKYKDRFVIPTSHREQVADLYSEQGSCGLSF
AGGPGSCMTIS

Sequences:

>Translated_491_residues
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSK
MKRLMNIFHNPDQPTIDDYFEPWNYDYETLTNSPQRKHQPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVK
KMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYFNWQTNKAE
KCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKVKEAASVENEKDLYESQLTVFLDPNDPEVAAEAKKQGIPEEWI
KAAQQSPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLSPIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDES
HIRLTLKKMAVMRTHMRALQINKEPNETVLKELGLTKQDVEDMYRLLAIAKYKDRFVIPTSHREQVADLYSEQGSCGLSF
AGGPGSCMTIS
>Mature_491_residues
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQEKYKGGWELKNGEIQLKSGSK
MKRLMNIFHNPDQPTIDDYFEPWNYDYETLTNSPQRKHQPVARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVK
KMEEEIKTDFENVFMMYLPRICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYFNWQTNKAE
KCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKVKEAASVENEKDLYESQLTVFLDPNDPEVAAEAKKQGIPEEWI
KAAQQSPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLSPIMNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDES
HIRLTLKKMAVMRTHMRALQINKEPNETVLKELGLTKQDVEDMYRLLAIAKYKDRFVIPTSHREQVADLYSEQGSCGLSF
AGGPGSCMTIS

Specific function: This Is A Second Nitrate Reductase Enzyme Which Can Substitute For The Nra Enzyme And Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=484, Percent_Identity=60.3305785123967, Blast_Score=649, Evalue=0.0,
Organism=Escherichia coli, GI1787478, Length=487, Percent_Identity=59.5482546201232, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=132, Percent_Identity=35.6060606060606, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787122, Length=132, Percent_Identity=35.6060606060606, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=37.2093023255814, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI2367345, Length=141, Percent_Identity=26.9503546099291, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1789370, Length=102, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=32.183908045977, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=31.0344827586207, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 56231; Mature: 56231

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
8.6 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
8.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQE
CEECCCCCCEEEHHHHHCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHH
KYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDDYFEPWNYDYETLTNSPQRKHQP
HHCCCCEECCCEEEECCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHCCCCHHHCCC
VARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVKKMEEEIKTDFENVFMMYLPR
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYFNWQTNKAE
HHHHHCCCHHHHCCCCCCCEEECCCCEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCC
KCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKVKEAASVENEKDLYESQLTVFLD
CHHEECHHHHCCCCHHHHHHHHHHHHEEEEEEECHHHHHHHHHCCCHHHHHHCCEEEEEC
PNDPEVAAEAKKQGIPEEWIKAAQQSPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLSPI
CCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEEECCHHHH
MNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDESHIRLTLKKMAVMRTHMRALQ
HHHHCCCCCCCCHHHHHHHHCCCEECHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHH
INKEPNETVLKELGLTKQDVEDMYRLLAIAKYKDRFVIPTSHREQVADLYSEQGSCGLSF
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHCCCCCCEEE
AGGPGSCMTIS
CCCCCCEEEEC
>Mature Secondary Structure
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQE
CEECCCCCCEEEHHHHHCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHH
KYKGGWELKNGEIQLKSGSKMKRLMNIFHNPDQPTIDDYFEPWNYDYETLTNSPQRKHQP
HHCCCCEECCCEEEECCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHCCCCHHHCCC
VARPKSAITGEFIDKIEWGPNWEDDLAGGHITGLQDPNVKKMEEEIKTDFENVFMMYLPR
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ICEHCMNPSCVSSCPSGAMYKREEDGIVLVDQNACRAWRFCVSSCPYKKVYFNWQTNKAE
HHHHHCCCHHHHCCCCCCCEEECCCCEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCC
KCTMCFPRIEAGMPTICSETCVGRIRYIGVMLYDADKVKEAASVENEKDLYESQLTVFLD
CHHEECHHHHCCCCHHHHHHHHHHHHEEEEEEECHHHHHHHHHCCCHHHHHHCCEEEEEC
PNDPEVAAEAKKQGIPEEWIKAAQQSPIYKMIIDWKIALPLHPEYRTMPMVWYIPPLSPI
CCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEEECCHHHH
MNMVEGKGSNWQAEEIFPAIDNMRIPIQYLANLLTAGDESHIRLTLKKMAVMRTHMRALQ
HHHHCCCCCCCCHHHHHHHHCCCEECHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHH
INKEPNETVLKELGLTKQDVEDMYRLLAIAKYKDRFVIPTSHREQVADLYSEQGSCGLSF
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHCCCCCCEEE
AGGPGSCMTIS
CCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]