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The map label for this gene is narI [H]

Identifier: 52143302

GI number: 52143302

Start: 2048829

End: 2049518

Strand: Direct

Name: narI [H]

Synonym: BCZK1935

Alternate gene names: 52143302

Gene position: 2048829-2049518 (Clockwise)

Preceding gene: 52143303

Following gene: 52143300

Centisome position: 38.65

GC content: 36.38

Gene sequence:

>690_bases
ATGATGGATCAATTTCTATGGGTATTGTTTCCCTATATCATTTTTGCAATCTTTATCGGTGGACATATTTTCAGATACAA
CTATGATCAATTTGGTTGGACATCAAAATCTAGTGAACTATTAGAAAAAAAAATGCTCCGAATCGGAAGTATATTATTCC
ACTTTGGGATTATGTTCGTAATTGGCGGGCATGTTATGGGGATTTTAATTCCAGAAGCTGTATACCGTTCCATAGGTATT
TCTGAGCATATGTATCATGTAGTAGCAATTAGTTTTGGGCTTCCAGCAGGGGTTGCTTCAATCATCGGTTTAATTATATT
AACGTACCGCCGCGTAACGGTAAAACGTATCATTGCAACGAGTACAAAGGGAGATTATATTGCGCTTATTCTATTACTTA
TCGTAATGCTAGCAGGACTTTCTTCAACGTTTTTAAATATCGACTCAAAAGGATTTGATTATCGTACAACGATCGGTCCA
TGGTTCCGAAGCCTCTTCGTTTTTCAACCGAAAGTTGATTATATGAAGGAAGTACCTGTATGGTTTAAAATTCATATTCT
TGCGGGAATGGGGCTTTTCGCAGTATGGCCATTTACTAGACTGGTACACGTATTTAGTGCCCCAATTAAATATGTAAGCC
GTAGCTATGTAATTTACAGAAGACGTATTCCAAATGAATTGAAAAAATAA

Upstream 100 bases:

>100_bases
TAAAAGAAGCAGATAGTATGTATGAACCAATTCTTTCGGCTGTTTTACTTGCTATCGATACATGGGGTGTACAGACAAAT
TAGAAAGGAGAGTTGTCAAA

Downstream 100 bases:

>100_bases
ATTAGTTTTATATGTTTCGTAATTTATAAGGAAAGCCGAGATCTAATTGGAGACCTCGGCTTTCCTTATAGGTATAAGTA
CCTAAATAAGACGCTAATCA

Product: nitrate reductase, gamma subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MMDQFLWVLFPYIIFAIFIGGHIFRYNYDQFGWTSKSSELLEKKMLRIGSILFHFGIMFVIGGHVMGILIPEAVYRSIGI
SEHMYHVVAISFGLPAGVASIIGLIILTYRRVTVKRIIATSTKGDYIALILLLIVMLAGLSSTFLNIDSKGFDYRTTIGP
WFRSLFVFQPKVDYMKEVPVWFKIHILAGMGLFAVWPFTRLVHVFSAPIKYVSRSYVIYRRRIPNELKK

Sequences:

>Translated_229_residues
MMDQFLWVLFPYIIFAIFIGGHIFRYNYDQFGWTSKSSELLEKKMLRIGSILFHFGIMFVIGGHVMGILIPEAVYRSIGI
SEHMYHVVAISFGLPAGVASIIGLIILTYRRVTVKRIIATSTKGDYIALILLLIVMLAGLSSTFLNIDSKGFDYRTTIGP
WFRSLFVFQPKVDYMKEVPVWFKIHILAGMGLFAVWPFTRLVHVFSAPIKYVSRSYVIYRRRIPNELKK
>Mature_229_residues
MMDQFLWVLFPYIIFAIFIGGHIFRYNYDQFGWTSKSSELLEKKMLRIGSILFHFGIMFVIGGHVMGILIPEAVYRSIGI
SEHMYHVVAISFGLPAGVASIIGLIILTYRRVTVKRIIATSTKGDYIALILLLIVMLAGLSSTFLNIDSKGFDYRTTIGP
WFRSLFVFQPKVDYMKEVPVWFKIHILAGMGLFAVWPFTRLVHVFSAPIKYVSRSYVIYRRRIPNELKK

Specific function: This Is A Second Nitrate Reductase Enzyme Which Can Substitute For The Nra Enzyme And Allows E.Coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Gamma Chain Is A Membrane-Embedded Heme-Iron Unit Resembling Cytochrome B, Which Trans

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787738, Length=223, Percent_Identity=36.322869955157, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787480, Length=226, Percent_Identity=33.1858407079646, Blast_Score=124, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 26359; Mature: 26359

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDQFLWVLFPYIIFAIFIGGHIFRYNYDQFGWTSKSSELLEKKMLRIGSILFHFGIMFV
CCHHHHHHHHHHHHHHHHHCCHHEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGGHVMGILIPEAVYRSIGISEHMYHVVAISFGLPAGVASIIGLIILTYRRVTVKRIIAT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
STKGDYIALILLLIVMLAGLSSTFLNIDSKGFDYRTTIGPWFRSLFVFQPKVDYMKEVPV
CCCCHHHHHHHHHHHHHHCCCHHHEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCH
WFKIHILAGMGLFAVWPFTRLVHVFSAPIKYVSRSYVIYRRRIPNELKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCC
>Mature Secondary Structure
MMDQFLWVLFPYIIFAIFIGGHIFRYNYDQFGWTSKSSELLEKKMLRIGSILFHFGIMFV
CCHHHHHHHHHHHHHHHHHCCHHEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGGHVMGILIPEAVYRSIGISEHMYHVVAISFGLPAGVASIIGLIILTYRRVTVKRIIAT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
STKGDYIALILLLIVMLAGLSSTFLNIDSKGFDYRTTIGPWFRSLFVFQPKVDYMKEVPV
CCCCHHHHHHHHHHHHHHCCCHHHEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCH
WFKIHILAGMGLFAVWPFTRLVHVFSAPIKYVSRSYVIYRRRIPNELKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 9384377; 7557333 [H]