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The map label for this gene is narK [H]

Identifier: 52143295

GI number: 52143295

Start: 2054781

End: 2055950

Strand: Direct

Name: narK [H]

Synonym: BCZK1942

Alternate gene names: 52143295

Gene position: 2054781-2055950 (Clockwise)

Preceding gene: 52143297

Following gene: 52143294

Centisome position: 38.76

GC content: 33.85

Gene sequence:

>1170_bases
ATGAAAAGTCCTAATTTTCAATTAAGTTTACAAACTTCTAATCTAATTATCGGTTTTATGGTGTGGGTTATTTTATCATC
ATTAATGCCCTATATTAAAGTGGATATCCCATTAACTGCGGGACAAATTTCTATGGTAACAGCAGTACCCGTTATTTTAG
GTTCTGTTCTTCGCATTCCAATTGGTTATTGGACAAATCGTTTCGGAGCAAGAAAATTATTCTTTATTAGTTTTATTCTA
TTATTATTTCCTGTTTTTTATATTAGTGTTGCCAATTCTATGATGGATTTAATTATTGGTGGATTATTTGTAGGAATCGG
TGGTGCTGTATTCTCTGTAGGAGTAACTTCTTTACCAAAATACTTTCCAAAAGAGAGTCATGGTTTTGTAAATGGTATTT
ACGGTGTCGGTAACGCTGGAACAGCAATTACTTCATTTTTAGCGCCTGTTATCGCAACTTCAGTTGGCTGGAGAACGACA
GTACAATGTTATTTAGTTTTACTTGCAGCGTTTGCACTTATGAACTTTTTATTAGGTGATCGTAAAGAGAAAAAAGTGAA
TACACCACTAATGGAACAAATAAAAGGTGTATATAAAAATGAGAAACTTTGGTTTTTATGTATCTTTTACTTTTTAACTT
TCGGATCATTTGTTGCATTTACAGTATATTTACCAAACTTTTTAGTATCTCATTTCGGATTAGAGAAAGTAGATGCAGGT
ATGCGGACAGCCGGATTCATCGTACTCGCAACAATTATGCGTCCGATTGGTGGTTGGCTCGGTGATAAGTTTAATCCATT
TAAAATATTAATCTTCGTATTTATCGGTTTAACACTTTCAGGTATTATTTTATCATTTATGCCAAGCATGAACGTATATA
CATTTGGTTGCCTATTAGTCGCATTTTGCGCAGGTATCGGTAATGGTACAATCTTCAAACTCGTTCCGATGTATTTCTCA
GAACAAGCTGGTATTGTAAATGGACTCGTTTCAGCTTTAGGCGGACTTGGAGGATTCTTCCCGCCATTAATTTTAACATT
ACTGTTCCAACTAACAGGTCATTATGCAATTGGATTTATGGCATTATCAGAAGTCGCACTTGCTTGTTTAATTATTACAG
TATGGATGTATAGTCAAGAAAAGCTGTTAGTGATGTTAAAAAATCATTAA

Upstream 100 bases:

>100_bases
ATTCCGCCAGTAAGTGGTGGTTGATTCTTTTCAGCCTAATCATGTAAGAACGTGATTAGGCTGGAGGGAATATAACTACG
TATACATAGGAGGGAACAGG

Downstream 100 bases:

>100_bases
TAAAGAAAAAGAGCCGTCCCAAAGTATTTTGGGATGGTTCTTTTCGTAATATAATAGGCTTCGCTATATTTTTTAGGACC
AGATTCTTTAGTAAGACTAA

Product: nitrite extrusion protein

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MKSPNFQLSLQTSNLIIGFMVWVILSSLMPYIKVDIPLTAGQISMVTAVPVILGSVLRIPIGYWTNRFGARKLFFISFIL
LLFPVFYISVANSMMDLIIGGLFVGIGGAVFSVGVTSLPKYFPKESHGFVNGIYGVGNAGTAITSFLAPVIATSVGWRTT
VQCYLVLLAAFALMNFLLGDRKEKKVNTPLMEQIKGVYKNEKLWFLCIFYFLTFGSFVAFTVYLPNFLVSHFGLEKVDAG
MRTAGFIVLATIMRPIGGWLGDKFNPFKILIFVFIGLTLSGIILSFMPSMNVYTFGCLLVAFCAGIGNGTIFKLVPMYFS
EQAGIVNGLVSALGGLGGFFPPLILTLLFQLTGHYAIGFMALSEVALACLIITVWMYSQEKLLVMLKNH

Sequences:

>Translated_389_residues
MKSPNFQLSLQTSNLIIGFMVWVILSSLMPYIKVDIPLTAGQISMVTAVPVILGSVLRIPIGYWTNRFGARKLFFISFIL
LLFPVFYISVANSMMDLIIGGLFVGIGGAVFSVGVTSLPKYFPKESHGFVNGIYGVGNAGTAITSFLAPVIATSVGWRTT
VQCYLVLLAAFALMNFLLGDRKEKKVNTPLMEQIKGVYKNEKLWFLCIFYFLTFGSFVAFTVYLPNFLVSHFGLEKVDAG
MRTAGFIVLATIMRPIGGWLGDKFNPFKILIFVFIGLTLSGIILSFMPSMNVYTFGCLLVAFCAGIGNGTIFKLVPMYFS
EQAGIVNGLVSALGGLGGFFPPLILTLLFQLTGHYAIGFMALSEVALACLIITVWMYSQEKLLVMLKNH
>Mature_389_residues
MKSPNFQLSLQTSNLIIGFMVWVILSSLMPYIKVDIPLTAGQISMVTAVPVILGSVLRIPIGYWTNRFGARKLFFISFIL
LLFPVFYISVANSMMDLIIGGLFVGIGGAVFSVGVTSLPKYFPKESHGFVNGIYGVGNAGTAITSFLAPVIATSVGWRTT
VQCYLVLLAAFALMNFLLGDRKEKKVNTPLMEQIKGVYKNEKLWFLCIFYFLTFGSFVAFTVYLPNFLVSHFGLEKVDAG
MRTAGFIVLATIMRPIGGWLGDKFNPFKILIFVFIGLTLSGIILSFMPSMNVYTFGCLLVAFCAGIGNGTIFKLVPMYFS
EQAGIVNGLVSALGGLGGFFPPLILTLLFQLTGHYAIGFMALSEVALACLIITVWMYSQEKLLVMLKNH

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=440, Percent_Identity=22.0454545454545, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787475, Length=423, Percent_Identity=24.822695035461, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42650; Mature: 42650

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSPNFQLSLQTSNLIIGFMVWVILSSLMPYIKVDIPLTAGQISMVTAVPVILGSVLRIP
CCCCCEEEEEECCHHHHHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHHH
IGYWTNRFGARKLFFISFILLLFPVFYISVANSMMDLIIGGLFVGIGGAVFSVGVTSLPK
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFPKESHGFVNGIYGVGNAGTAITSFLAPVIATSVGWRTTVQCYLVLLAAFALMNFLLGD
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
RKEKKVNTPLMEQIKGVYKNEKLWFLCIFYFLTFGSFVAFTVYLPNFLVSHFGLEKVDAG
CCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
MRTAGFIVLATIMRPIGGWLGDKFNPFKILIFVFIGLTLSGIILSFMPSMNVYTFGCLLV
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
AFCAGIGNGTIFKLVPMYFSEQAGIVNGLVSALGGLGGFFPPLILTLLFQLTGHYAIGFM
HHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ALSEVALACLIITVWMYSQEKLLVMLKNH
HHHHHHHHHHHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]