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The map label for this gene is nasF [H]

Identifier: 52143291

GI number: 52143291

Start: 2059290

End: 2060714

Strand: Reverse

Name: nasF [H]

Synonym: BCZK1947

Alternate gene names: 52143291

Gene position: 2060714-2059290 (Counterclockwise)

Preceding gene: 52143288

Following gene: 52143290

Centisome position: 38.87

GC content: 36.42

Gene sequence:

>1425_bases
ATGAACGGATATGTATATTTAGTTGGTGCAGGACCTGGTGATGAAGGGCTTATTACAAAGAAAGCGATAGAGTGTTTAAA
ACGTGCAGATATTGTTTTATATGACCGTTTACTAAACCCTTTCTTTCTTAGCTATACAAAACAAACATGTGAACTTATGT
ATTGCGGAAAAATGCCAAAGAATCATATTATGCGACAAGAAATGATTAACGCACACCTTCTTCAATTTGCAAAAGAGGGA
AAAATTGTTGTCCGATTAAAAGGCGGAGATCCATCTATTTTTGGCCGTGTTGGTGAAGAAGCAGAAACTTTAGCAGCAGC
AAATATTCCATATGAAATTGTACCAGGCATTACATCTAGCATCGCCGCTAGTAGCTATGCAGGTATCCCCCTTACCCACC
GTAACTACAGTAATAGCGTTACTTTACTAACCGGACATGCAAAAGGTCCTTTAACCGATCATGGAAAATATAATTCATCT
CACAATAGCGATACGATTGCCTACTACATGGGTATAAAAAACTTACCTACAATTTGCGAAAACTTACGACAAGCAGGAAA
GAAAGAAGATACGCCAGTAGCAGTCATTGAATGGGGTACAACTGGTAAACAGCGCGTTGTCACAGGGACACTATCTACCA
TTGTTTCTATCGTCAAAAATGAAAATATTTCTAACCCTTCTATGACAATTGTTGGTGATGTCGTTTCCTTACGAGATCAA
ATCGCTTGGAAAGAACGTAAACCATTACACGGTAAAAAAGTTTTATTTACATCCGCAACAAATAAAACAAGTGTAATGAA
GCAAAGGTTACAAGAAGCTGGTGCAGAAATATATCAAATTCCAACTTTCAAAAAGGAAGAATATACATTAACACTTGAAC
AAATCAATGAAATTTTTAACGTTGATCGCCTTGTTTTTTGTTCAGCTGAAAGTGTAGAAATTTTGATGCAATCATGTAGT
AAATACCAGAAAGATATTCGTTCCTTACAGGCAGAGCTGCAACATATGAACGTTGCCACTCAAGAAAAACTTATGCAATA
TGGCCTACTAAGTAAAGAAGCAAAGTTCTCTTCCGATACAACTGTTTATTTAGGCAGAAATATTAATCGGATTGCTTTTA
TTCAAGAGAAAATTGGTGCCGGCTCCTATATGATGACTCATAAGTATACAATTGATCATCGTTTCGATGAAGTCCATTCT
CGTATGCTTTCAGAATTCTTATGGGATAGCATTGTATTTGAAGGACGCGCTTCTATTGATACGTTTCTAGCAGAAGTAAA
ACGTCTTGGCTTTATCGATATTGTTACTCTCCCATTCTCGTATACCGACGTTCCAACTTTACACTATGCAAATAAAGTCG
GGTTTCATAACGTAGATCATCAATTACAAGAAACTCTTATGAAAAAAGATATGGTAAGACAATGA

Upstream 100 bases:

>100_bases
GTAGAAGTCATTGATGGTGACATTTATATATATATGTAATTTACAAAAGGAAGCCTTCCCCTGGCTTCCTTTTAAATAAA
ACTAGATGAGGTGCAATTAT

Downstream 100 bases:

>100_bases
AAGGAATTGTATATGTTGGACACGGGAGCCGGTTACAAGAAGGGAACGAACAATTCATTCACTTTATTCAATCTGTTATG
AAAGAACGTAACGAAAGAAT

Product: bifunctional uroporphyrinogen-III methyltransferase/uroporphyrinogen-III synthase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MNGYVYLVGAGPGDEGLITKKAIECLKRADIVLYDRLLNPFFLSYTKQTCELMYCGKMPKNHIMRQEMINAHLLQFAKEG
KIVVRLKGGDPSIFGRVGEEAETLAAANIPYEIVPGITSSIAASSYAGIPLTHRNYSNSVTLLTGHAKGPLTDHGKYNSS
HNSDTIAYYMGIKNLPTICENLRQAGKKEDTPVAVIEWGTTGKQRVVTGTLSTIVSIVKNENISNPSMTIVGDVVSLRDQ
IAWKERKPLHGKKVLFTSATNKTSVMKQRLQEAGAEIYQIPTFKKEEYTLTLEQINEIFNVDRLVFCSAESVEILMQSCS
KYQKDIRSLQAELQHMNVATQEKLMQYGLLSKEAKFSSDTTVYLGRNINRIAFIQEKIGAGSYMMTHKYTIDHRFDEVHS
RMLSEFLWDSIVFEGRASIDTFLAEVKRLGFIDIVTLPFSYTDVPTLHYANKVGFHNVDHQLQETLMKKDMVRQ

Sequences:

>Translated_474_residues
MNGYVYLVGAGPGDEGLITKKAIECLKRADIVLYDRLLNPFFLSYTKQTCELMYCGKMPKNHIMRQEMINAHLLQFAKEG
KIVVRLKGGDPSIFGRVGEEAETLAAANIPYEIVPGITSSIAASSYAGIPLTHRNYSNSVTLLTGHAKGPLTDHGKYNSS
HNSDTIAYYMGIKNLPTICENLRQAGKKEDTPVAVIEWGTTGKQRVVTGTLSTIVSIVKNENISNPSMTIVGDVVSLRDQ
IAWKERKPLHGKKVLFTSATNKTSVMKQRLQEAGAEIYQIPTFKKEEYTLTLEQINEIFNVDRLVFCSAESVEILMQSCS
KYQKDIRSLQAELQHMNVATQEKLMQYGLLSKEAKFSSDTTVYLGRNINRIAFIQEKIGAGSYMMTHKYTIDHRFDEVHS
RMLSEFLWDSIVFEGRASIDTFLAEVKRLGFIDIVTLPFSYTDVPTLHYANKVGFHNVDHQLQETLMKKDMVRQ
>Mature_474_residues
MNGYVYLVGAGPGDEGLITKKAIECLKRADIVLYDRLLNPFFLSYTKQTCELMYCGKMPKNHIMRQEMINAHLLQFAKEG
KIVVRLKGGDPSIFGRVGEEAETLAAANIPYEIVPGITSSIAASSYAGIPLTHRNYSNSVTLLTGHAKGPLTDHGKYNSS
HNSDTIAYYMGIKNLPTICENLRQAGKKEDTPVAVIEWGTTGKQRVVTGTLSTIVSIVKNENISNPSMTIVGDVVSLRDQ
IAWKERKPLHGKKVLFTSATNKTSVMKQRLQEAGAEIYQIPTFKKEEYTLTLEQINEIFNVDRLVFCSAESVEILMQSCS
KYQKDIRSLQAELQHMNVATQEKLMQYGLLSKEAKFSSDTTVYLGRNINRIAFIQEKIGAGSYMMTHKYTIDHRFDEVHS
RMLSEFLWDSIVFEGRASIDTFLAEVKRLGFIDIVTLPFSYTDVPTLHYANKVGFHNVDHQLQETLMKKDMVRQ

Specific function: Multifunctional Enzyme That Catalyze The Sam-Dependent Methylation Of Uroporphyrinogen III At Position C-2 And C-7 To Form Precorrin-2 And Then Position C-12 Or C-18 To Form Trimethylpyrrocorphin 2. It Also Catalyzes The Conversion Of Precorrin-2 Into Sir

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=242, Percent_Identity=45.8677685950413, Blast_Score=214, Evalue=8e-57,
Organism=Saccharomyces cerevisiae, GI6322922, Length=249, Percent_Identity=31.7269076305221, Blast_Score=114, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 53364; Mature: 53364

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGYVYLVGAGPGDEGLITKKAIECLKRADIVLYDRLLNPFFLSYTKQTCELMYCGKMPK
CCCEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCH
NHIMRQEMINAHLLQFAKEGKIVVRLKGGDPSIFGRVGEEAETLAAANIPYEIVPGITSS
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCHHHHHHHCCCCHHCCCCCHHH
IAASSYAGIPLTHRNYSNSVTLLTGHAKGPLTDHGKYNSSHNSDTIAYYMGIKNLPTICE
HHHCCCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHH
NLRQAGKKEDTPVAVIEWGTTGKQRVVTGTLSTIVSIVKNENISNPSMTIVGDVVSLRDQ
HHHHCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHH
IAWKERKPLHGKKVLFTSATNKTSVMKQRLQEAGAEIYQIPTFKKEEYTLTLEQINEIFN
HHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEECCCCCCCCEEEEHHHHHHHHC
VDRLVFCSAESVEILMQSCSKYQKDIRSLQAELQHMNVATQEKLMQYGLLSKEAKFSSDT
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCC
TVYLGRNINRIAFIQEKIGAGSYMMTHKYTIDHRFDEVHSRMLSEFLWDSIVFEGRASID
EEEECCCCCCEEEEHHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
TFLAEVKRLGFIDIVTLPFSYTDVPTLHYANKVGFHNVDHQLQETLMKKDMVRQ
HHHHHHHHCCCEEEEECCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNGYVYLVGAGPGDEGLITKKAIECLKRADIVLYDRLLNPFFLSYTKQTCELMYCGKMPK
CCCEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCH
NHIMRQEMINAHLLQFAKEGKIVVRLKGGDPSIFGRVGEEAETLAAANIPYEIVPGITSS
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCHHHHHHHCCCCHHCCCCCHHH
IAASSYAGIPLTHRNYSNSVTLLTGHAKGPLTDHGKYNSSHNSDTIAYYMGIKNLPTICE
HHHCCCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHH
NLRQAGKKEDTPVAVIEWGTTGKQRVVTGTLSTIVSIVKNENISNPSMTIVGDVVSLRDQ
HHHHCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHH
IAWKERKPLHGKKVLFTSATNKTSVMKQRLQEAGAEIYQIPTFKKEEYTLTLEQINEIFN
HHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEECCCCCCCCEEEEHHHHHHHHC
VDRLVFCSAESVEILMQSCSKYQKDIRSLQAELQHMNVATQEKLMQYGLLSKEAKFSSDT
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCC
TVYLGRNINRIAFIQEKIGAGSYMMTHKYTIDHRFDEVHSRMLSEFLWDSIVFEGRASID
EEEECCCCCCEEEEHHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
TFLAEVKRLGFIDIVTLPFSYTDVPTLHYANKVGFHNVDHQLQETLMKKDMVRQ
HHHHHHHHCCCEEEEECCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]