Click here to switch to the map view.

The map label for this gene is nirB [H]

Identifier: 52143289

GI number: 52143289

Start: 2061109

End: 2063514

Strand: Reverse

Name: nirB [H]

Synonym: BCZK1949

Alternate gene names: 52143289

Gene position: 2063514-2061109 (Counterclockwise)

Preceding gene: 52143287

Following gene: 52143288

Centisome position: 38.93

GC content: 37.95

Gene sequence:

>2406_bases
ATGAAAAAACGTTTAGTTATGATCGGAAATGGTATGGCTGGCATACGTTGTATGGAAGAAATATTAAAACATGATTGTGA
TTCATATGAGATTACTATTTTTGGAGATGAACCACACCCAAACTATAATCGCATTATGCTCTCTCATGTTTTACAGGGTA
AAACAAATATACAAAATATCATTATGAATGAATACAGTTGGTATGAAGAAAACGAAATAACTTTGTATACAAATGAAAGA
GTTCAAAGTATTGACCGAGAAGAAAAAGTTATTATTACAGAAAAGAATCGTACTCTTACATATGACAAGCTTATTATTGC
AACAGGTTCTAGTGCTTTTATTTTACCTGTTGAAGGTTCCACTCTTCCTGGTGTAACAGGTTTTCGAACTATTGAAGATA
CACAGTTTATGATGGATACTGCTAAAGAAAAGAAAAAGGCCGTTGTCATTGGTGGTGGATTACTTGGTTTAGAAGCAGCA
CGAGGTCTTATTGACTTAGGAATGGACGTACATGTTGTTCATTTAATGCCTAGCTTAATGGAGCAACAACTAGATACGAA
AGCAGCTTCTCTACTGCGTGAAGATTTAGAAGCACAAGGCATGAAGTTTCTTATGGAAAAGAAAACTGTAAAAATTCTTG
GTACAAACCATGTTGAGGGCATTCAATTTGAAGATGGTGAAGTTGTAGATTGTGATTTAATCGTAATGGCTGTCGGAATC
CGACCAAATACGCAAATAGCAAGAGATGCTGGTTTAATTGTCAATCGCGGCATTGTAGTCAATGACTATATGCTAACAAA
TGATGAGTCCATTTATGCAGTTGGCGAATGTGCAGAACATGACGGTATCGCATATGGACTTGTTGCCCCTCTTTATGAAC
AAGGTGCAATATTAGCGAAACATATATCGAATTTACAAACTGATGGATATTCGGGCAGTATCGTCGGTACGCAATTAAAA
GTTGCTGGTTGTGATTTATTCTCTGCAGGCCAAATTTATGAAGATGATCAAACGAAAGCAATATCCATCTTTAATGAATG
CAAACGTTCCTATAAAAAAGTATTAATTCGTGACAATAAAGTCGTCGGTATCGTTTTATATGGTGACACAGCTGATGGTA
CACGCCTCTTTAGCATGTTAAAGAAAGAAGAAGATATACAAGAATATACACCAGCGTCCATTCTTCACAAAGCTGGTGAA
GAAATTGAACTTGATGTTGCTACAATGAGTGCGGATGACACGATTTGTGGATGTAATGGTGTTACGAAAGGAACAATCGT
TCATGCCATTTTAGAACAAGAGTTAACGACTTTTGAAGAAGTTAAAGGCTGTACGAAAGCCGCAGGGTCTTGTGGTAAAT
GTCGTCCGCTTGTAGAACAAGTTTTATCTCATACACTCGGAGATGCTTTTGATGCCTCAGCCCAGTCTGCTGGTATGTGC
GGATGTACACCTTTATCACGTGATGAAGTCGTAGCGGCAATTCATGAAAAAGGCTTAAAATCACCAAAAGAAGTACGAAA
TGTTCTTGGTTTTGTACATGAAGATGGCTGTTCGAAATGTCGTCCTGCTTTGAACTACTATTTACGTATGGCGATTCCAG
AAGAATATGAAGATGATAAAGCCTCCCGTTTCGTTAATGAAAGAATGAATGGTAACATCCAGCACGATGGTACATTCTCT
GTTATTCCACGTATGTACGGAGGCGTTACAACAGCAGATGATTTAATGAAAATTGCTGAAGTTGCGAAAAAGTATGATGT
TCCACTTGTGAAAATTACCGGTGCAAGTCGAATTGGCTTATACGGTGTTAAGAAACAAGATTTACCTAACGTATGGGCTG
ACTTAAATATGACTTCTGGATATGCGTATTCAAAATCTCTTCGTAATGTAAAATCATGTGTTGGCTCTCGTTTCTGCCGT
TTTGGTACGAAAGATTCATTAGGACTTGGTATGCTCCTTGAACAATCATTAGAAATGGTAGATACACCTCATAAAATGAA
GATGGGTGTAACGGGCTGTCCGCGTAACTGTGCGGAAGTACTTACGAAAGATTTTGGCGTTGTTTGTGTCGAAAATGGAT
ACCAACTTTATATTGGTGGAAATGGTGGTACAGAAGTACGTGAAGCTGATTTTGTAATGATTGTCCCTACTGAAGATGAT
GTTCTTCACATCGCTACAGCTTACATGCAATATTATCGTGAAACTGGTATTTACGGAGAGCGTACTGCCTACTGGACAGA
ACGATTAGGCTTCGATCACATAAAAGAAATACTGCAAGATGCAAATATGGTCACTAAGTTAAATGAACGTTTCCAAACGG
CCCGTGGCACATATAAAGAAGCTTGGGGACAAGCACTAGAAACGAAATCATTAAAAGCGATGTATGAAGTAGAAACTGTG
AAATAA

Upstream 100 bases:

>100_bases
TCAAAAGTGTGATAAATATCACATCTCCCCCTTTTTTGATTTTCTATAATAGAAATATAATCCTTTGCTCAATACTTCAC
ATAAAAAAGGGGAAACGAAA

Downstream 100 bases:

>100_bases
GGAGCTGTGATCTATATGATACAAACGAAAGATAAAATAAAAGTTATGCGTGCAGAAGATCTTCCTATTCAAATCGGTAA
AGAGGTCCAAATGAAAGGTA

Product: nitrite reductase [NAD(P)H], large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MKKRLVMIGNGMAGIRCMEEILKHDCDSYEITIFGDEPHPNYNRIMLSHVLQGKTNIQNIIMNEYSWYEENEITLYTNER
VQSIDREEKVIITEKNRTLTYDKLIIATGSSAFILPVEGSTLPGVTGFRTIEDTQFMMDTAKEKKKAVVIGGGLLGLEAA
RGLIDLGMDVHVVHLMPSLMEQQLDTKAASLLREDLEAQGMKFLMEKKTVKILGTNHVEGIQFEDGEVVDCDLIVMAVGI
RPNTQIARDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAKHISNLQTDGYSGSIVGTQLK
VAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNKVVGIVLYGDTADGTRLFSMLKKEEDIQEYTPASILHKAGE
EIELDVATMSADDTICGCNGVTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFVHEDGCSKCRPALNYYLRMAIPEEYEDDKASRFVNERMNGNIQHDGTFS
VIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGLYGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCR
FGTKDSLGLGMLLEQSLEMVDTPHKMKMGVTGCPRNCAEVLTKDFGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
VLHIATAYMQYYRETGIYGERTAYWTERLGFDHIKEILQDANMVTKLNERFQTARGTYKEAWGQALETKSLKAMYEVETV
K

Sequences:

>Translated_801_residues
MKKRLVMIGNGMAGIRCMEEILKHDCDSYEITIFGDEPHPNYNRIMLSHVLQGKTNIQNIIMNEYSWYEENEITLYTNER
VQSIDREEKVIITEKNRTLTYDKLIIATGSSAFILPVEGSTLPGVTGFRTIEDTQFMMDTAKEKKKAVVIGGGLLGLEAA
RGLIDLGMDVHVVHLMPSLMEQQLDTKAASLLREDLEAQGMKFLMEKKTVKILGTNHVEGIQFEDGEVVDCDLIVMAVGI
RPNTQIARDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAKHISNLQTDGYSGSIVGTQLK
VAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNKVVGIVLYGDTADGTRLFSMLKKEEDIQEYTPASILHKAGE
EIELDVATMSADDTICGCNGVTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFVHEDGCSKCRPALNYYLRMAIPEEYEDDKASRFVNERMNGNIQHDGTFS
VIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGLYGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCR
FGTKDSLGLGMLLEQSLEMVDTPHKMKMGVTGCPRNCAEVLTKDFGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
VLHIATAYMQYYRETGIYGERTAYWTERLGFDHIKEILQDANMVTKLNERFQTARGTYKEAWGQALETKSLKAMYEVETV
K
>Mature_801_residues
MKKRLVMIGNGMAGIRCMEEILKHDCDSYEITIFGDEPHPNYNRIMLSHVLQGKTNIQNIIMNEYSWYEENEITLYTNER
VQSIDREEKVIITEKNRTLTYDKLIIATGSSAFILPVEGSTLPGVTGFRTIEDTQFMMDTAKEKKKAVVIGGGLLGLEAA
RGLIDLGMDVHVVHLMPSLMEQQLDTKAASLLREDLEAQGMKFLMEKKTVKILGTNHVEGIQFEDGEVVDCDLIVMAVGI
RPNTQIARDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAKHISNLQTDGYSGSIVGTQLK
VAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNKVVGIVLYGDTADGTRLFSMLKKEEDIQEYTPASILHKAGE
EIELDVATMSADDTICGCNGVTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFVHEDGCSKCRPALNYYLRMAIPEEYEDDKASRFVNERMNGNIQHDGTFS
VIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGLYGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCR
FGTKDSLGLGMLLEQSLEMVDTPHKMKMGVTGCPRNCAEVLTKDFGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
VLHIATAYMQYYRETGIYGERTAYWTERLGFDHIKEILQDANMVTKLNERFQTARGTYKEAWGQALETKSLKAMYEVETV
K

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=276, Percent_Identity=27.1739130434783, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI226437568, Length=276, Percent_Identity=27.1739130434783, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI21389617, Length=276, Percent_Identity=27.1739130434783, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI22202629, Length=266, Percent_Identity=26.6917293233083, Blast_Score=68, Evalue=4e-11,
Organism=Homo sapiens, GI4757732, Length=266, Percent_Identity=26.6917293233083, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1789765, Length=816, Percent_Identity=35.2941176470588, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1789065, Length=309, Percent_Identity=30.0970873786408, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1788892, Length=280, Percent_Identity=24.6428571428571, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17559934, Length=404, Percent_Identity=22.7722772277228, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32565766, Length=210, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32564386, Length=370, Percent_Identity=24.0540540540541, Blast_Score=66, Evalue=9e-11,
Organism=Drosophila melanogaster, GI281359715, Length=281, Percent_Identity=24.1992882562278, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281359713, Length=281, Percent_Identity=24.1992882562278, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24639250, Length=281, Percent_Identity=24.1992882562278, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI18543267, Length=281, Percent_Identity=24.1992882562278, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24639252, Length=281, Percent_Identity=24.1992882562278, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24639257, Length=281, Percent_Identity=24.1992882562278, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585130, Length=347, Percent_Identity=22.7665706051873, Blast_Score=93, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 88676; Mature: 88676

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRLVMIGNGMAGIRCMEEILKHDCDSYEITIFGDEPHPNYNRIMLSHVLQGKTNIQNI
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCHHHHH
IMNEYSWYEENEITLYTNERVQSIDREEKVIITEKNRTLTYDKLIIATGSSAFILPVEGS
HHCCCCCCCCCCEEEEECHHHHHCCCCCEEEEEECCCEEEEEEEEEEECCCEEEEEECCC
TLPGVTGFRTIEDTQFMMDTAKEKKKAVVIGGGLLGLEAARGLIDLGMDVHVVHLMPSLM
CCCCCCCCEEECHHHHHHHHHHCCCCEEEEECCHHHHHHHCHHHHCCCCEEHHHHHHHHH
EQQLDTKAASLLREDLEAQGMKFLMEKKTVKILGTNHVEGIQFEDGEVVDCDLIVMAVGI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCEEEEEEEEEEECC
RPNTQIARDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAK
CCCCCHHHCCCEEEECCEEEEEEEEECCCCEEEEECCCCCCCEEEHHHHHHHHCCHHHHH
HISNLQTDGYSGSIVGTQLKVAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNK
HHHHCCCCCCCCCEEEEEEEEECCCEECCCCEECCCCHHHHHHHHHHHHHHHHEEEECCC
VVGIVLYGDTADGTRLFSMLKKEEDIQEYTPASILHKAGEEIELDVATMSADDTICGCNG
EEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEEECCCCCEECCCC
VTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCCC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFVHEDGCSKCRPALNYYLRMAIPEEYEDDK
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCHH
ASRFVNERMNGNIQHDGTFSVIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGL
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEECE
YGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCRFGTKDSLGLGMLLEQSLEMV
ECCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DTPHKMKMGVTGCPRNCAEVLTKDFGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
CCCCHHCCCCCCCCHHHHHHHHHCCCEEEEECCEEEEEECCCCCCCCCCCEEEEEECCCC
VLHIATAYMQYYRETGIYGERTAYWTERLGFDHIKEILQDANMVTKLNERFQTARGTYKE
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AWGQALETKSLKAMYEVETVK
HHHHHHHHHHHHHHEEEEECC
>Mature Secondary Structure
MKKRLVMIGNGMAGIRCMEEILKHDCDSYEITIFGDEPHPNYNRIMLSHVLQGKTNIQNI
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCHHHHH
IMNEYSWYEENEITLYTNERVQSIDREEKVIITEKNRTLTYDKLIIATGSSAFILPVEGS
HHCCCCCCCCCCEEEEECHHHHHCCCCCEEEEEECCCEEEEEEEEEEECCCEEEEEECCC
TLPGVTGFRTIEDTQFMMDTAKEKKKAVVIGGGLLGLEAARGLIDLGMDVHVVHLMPSLM
CCCCCCCCEEECHHHHHHHHHHCCCCEEEEECCHHHHHHHCHHHHCCCCEEHHHHHHHHH
EQQLDTKAASLLREDLEAQGMKFLMEKKTVKILGTNHVEGIQFEDGEVVDCDLIVMAVGI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCEEEEEEEEEEECC
RPNTQIARDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAK
CCCCCHHHCCCEEEECCEEEEEEEEECCCCEEEEECCCCCCCEEEHHHHHHHHCCHHHHH
HISNLQTDGYSGSIVGTQLKVAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNK
HHHHCCCCCCCCCEEEEEEEEECCCEECCCCEECCCCHHHHHHHHHHHHHHHHEEEECCC
VVGIVLYGDTADGTRLFSMLKKEEDIQEYTPASILHKAGEEIELDVATMSADDTICGCNG
EEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEEECCCCCEECCCC
VTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCCC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFVHEDGCSKCRPALNYYLRMAIPEEYEDDK
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCHH
ASRFVNERMNGNIQHDGTFSVIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGL
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEECE
YGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCRFGTKDSLGLGMLLEQSLEMV
ECCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DTPHKMKMGVTGCPRNCAEVLTKDFGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
CCCCHHCCCCCCCCHHHHHHHHHCCCEEEEECCEEEEEECCCCCCCCCCCEEEEEECCCC
VLHIATAYMQYYRETGIYGERTAYWTERLGFDHIKEILQDANMVTKLNERFQTARGTYKE
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AWGQALETKSLKAMYEVETVK
HHHHHHHHHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]