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The map label for this gene is murF [H]

Identifier: 52143257

GI number: 52143257

Start: 2098412

End: 2099776

Strand: Direct

Name: murF [H]

Synonym: BCZK1980

Alternate gene names: 52143257

Gene position: 2098412-2099776 (Clockwise)

Preceding gene: 52143259

Following gene: 52143256

Centisome position: 39.59

GC content: 33.19

Gene sequence:

>1365_bases
TTGATTCAAATGAATTTGAAAAAACTTGAAGAGGTAATAAATGGAGAAGGATTACACGAATCGTTTCATCATTTAGAAAT
ACATGGTGTCTGCATAGATTCCAAAAACATGAAAGAAGGAAATGTATTTGTTCCAATCATTAGGGTGAAGGATGGGCATG
AGTATGTGAAAGAGGCAATGGATAATGGAGCTATCTCTTCCTTATGGAAAAAGTCTTACGGTACTCCGCCAAAAGGGATT
CCAATTATATTTGTAGATGACACTTTGTTTGCTTTGCAACAATTGGCACAATTTTATCGAAGAGAGATAAATGTAAAAGT
AATCGGAATTACCGGTAGTAATGGCAAAACTACGGTAAAAGATATAATAGAAGGTATTTTAAGGTCAACTTATAGAGTGC
ATAAAACAAAAGGTAATTTTAATAGTCAAATCGGTTTACCGTTAACAATTTTAGAGATGAAACGAGATACAGAGTTTTTA
ATACTTGAAATGGGGATGAGTGAAAAAGGACAAATTCGAAATTTATCACGAATCGCACAGCCAGATGTAGCGGTGATTAC
AATGATTGGACAGTCTCATTTAGAAACGCTTGGATCAAGAAAAGAGATTGCGAAGGCTAAATTAGAGATTATTGACGGGT
TAAGAGAAAATGGTTTGTTCTTATATAATGGTGATGAGCCATTGTTTTCTCGAAATAATAAAACGTCGGGTGTAAATTGT
AAGACATTTGGTGAAAAGGATACAAATGATTTGTTTCCAACAAACGTACAATTAGATGAATATGGTGTTCATTTTAAATT
AAATCATACAAAAATACAGTACGATGTTCCCTTACATGGAAAACATAATATTTTTAATACGATTGTTGGAATCGCTGTAG
GTCAATTTTACAAAGTCCCTACAGAAAAAATACAAGAAGCATTGCAACAAATAAATATTACACAAATGCGCTTTCAGTTT
TTAACCGCTAAAATAGGTTTTACAATTATTAACGATGCATGGAATGCCAGCCCTTCCTCCATGAAGGCTGCAATTGAAAC
GTTACAGAAGTTAAATGCCTATAAGAAAAAAATTATAGTGATTGGGGATATGTTAGAGTTAGGGAAGAAAGCTGAAACAT
ATCATAGAGAAATTGGTAAAATGTTAAATGAAGAGAGCATCCAATATGTATTTACTTATGGAGAGTTAGCTAAAATTGTA
GCGGAAGAAGCAAGCAAGAAATATGATACAGGAAAAGTACAGTCATTTGATAATAAAGCCGAGATAGCAGAGGAAGTATT
AAAAGTAATAACGAAAAAGGATGTCGTATTATTGAAAGGTTCACGTGGAATGGCTTTAGAGGAAATTGTACAAAACTGGA
TGTGA

Upstream 100 bases:

>100_bases
AACTTATTGTTTTTTTCATATAAACACCTCACTATAAAATTTTTATTAATATGCGTAAATAAAACTGAAAATATGTAATA
AAATGGAAGTTGATGATAAT

Downstream 100 bases:

>100_bases
AAAATTAAAATATATTTAAAAGTGGTTGCATTTTATAGTGTGAAGAGATAGAATACTCTGTAAATATAAAAAGTGATGAT
CAGGAAAAGTACATGATGCA

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase.

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MIQMNLKKLEEVINGEGLHESFHHLEIHGVCIDSKNMKEGNVFVPIIRVKDGHEYVKEAMDNGAISSLWKKSYGTPPKGI
PIIFVDDTLFALQQLAQFYRREINVKVIGITGSNGKTTVKDIIEGILRSTYRVHKTKGNFNSQIGLPLTILEMKRDTEFL
ILEMGMSEKGQIRNLSRIAQPDVAVITMIGQSHLETLGSRKEIAKAKLEIIDGLRENGLFLYNGDEPLFSRNNKTSGVNC
KTFGEKDTNDLFPTNVQLDEYGVHFKLNHTKIQYDVPLHGKHNIFNTIVGIAVGQFYKVPTEKIQEALQQINITQMRFQF
LTAKIGFTIINDAWNASPSSMKAAIETLQKLNAYKKKIIVIGDMLELGKKAETYHREIGKMLNEESIQYVFTYGELAKIV
AEEASKKYDTGKVQSFDNKAEIAEEVLKVITKKDVVLLKGSRGMALEEIVQNWM

Sequences:

>Translated_454_residues
MIQMNLKKLEEVINGEGLHESFHHLEIHGVCIDSKNMKEGNVFVPIIRVKDGHEYVKEAMDNGAISSLWKKSYGTPPKGI
PIIFVDDTLFALQQLAQFYRREINVKVIGITGSNGKTTVKDIIEGILRSTYRVHKTKGNFNSQIGLPLTILEMKRDTEFL
ILEMGMSEKGQIRNLSRIAQPDVAVITMIGQSHLETLGSRKEIAKAKLEIIDGLRENGLFLYNGDEPLFSRNNKTSGVNC
KTFGEKDTNDLFPTNVQLDEYGVHFKLNHTKIQYDVPLHGKHNIFNTIVGIAVGQFYKVPTEKIQEALQQINITQMRFQF
LTAKIGFTIINDAWNASPSSMKAAIETLQKLNAYKKKIIVIGDMLELGKKAETYHREIGKMLNEESIQYVFTYGELAKIV
AEEASKKYDTGKVQSFDNKAEIAEEVLKVITKKDVVLLKGSRGMALEEIVQNWM
>Mature_454_residues
MIQMNLKKLEEVINGEGLHESFHHLEIHGVCIDSKNMKEGNVFVPIIRVKDGHEYVKEAMDNGAISSLWKKSYGTPPKGI
PIIFVDDTLFALQQLAQFYRREINVKVIGITGSNGKTTVKDIIEGILRSTYRVHKTKGNFNSQIGLPLTILEMKRDTEFL
ILEMGMSEKGQIRNLSRIAQPDVAVITMIGQSHLETLGSRKEIAKAKLEIIDGLRENGLFLYNGDEPLFSRNNKTSGVNC
KTFGEKDTNDLFPTNVQLDEYGVHFKLNHTKIQYDVPLHGKHNIFNTIVGIAVGQFYKVPTEKIQEALQQINITQMRFQF
LTAKIGFTIINDAWNASPSSMKAAIETLQKLNAYKKKIIVIGDMLELGKKAETYHREIGKMLNEESIQYVFTYGELAKIV
AEEASKKYDTGKVQSFDNKAEIAEEVLKVITKKDVVLLKGSRGMALEEIVQNWM

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=458, Percent_Identity=28.1659388646288, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 51283; Mature: 51283

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQMNLKKLEEVINGEGLHESFHHLEIHGVCIDSKNMKEGNVFVPIIRVKDGHEYVKEAM
CCCCCHHHHHHHHCCCCCCCHHHEEEEEEEEECCCCCCCCCEEEEEEEECCCHHHHHHHH
DNGAISSLWKKSYGTPPKGIPIIFVDDTLFALQQLAQFYRREINVKVIGITGSNGKTTVK
CCCHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHH
DIIEGILRSTYRVHKTKGNFNSQIGLPLTILEMKRDTEFLILEMGMSEKGQIRNLSRIAQ
HHHHHHHHHHHEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHCC
PDVAVITMIGQSHLETLGSRKEIAKAKLEIIDGLRENGLFLYNGDEPLFSRNNKTSGVNC
CCEEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCE
KTFGEKDTNDLFPTNVQLDEYGVHFKLNHTKIQYDVPLHGKHNIFNTIVGIAVGQFYKVP
EECCCCCCCCCCCCCEEEECCCEEEEEECEEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
TEKIQEALQQINITQMRFQFLTAKIGFTIINDAWNASPSSMKAAIETLQKLNAYKKKIIV
HHHHHHHHHHCCHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEE
IGDMLELGKKAETYHREIGKMLNEESIQYVFTYGELAKIVAEEASKKYDTGKVQSFDNKA
EECHHHHCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCHH
EIAEEVLKVITKKDVVLLKGSRGMALEEIVQNWM
HHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCC
>Mature Secondary Structure
MIQMNLKKLEEVINGEGLHESFHHLEIHGVCIDSKNMKEGNVFVPIIRVKDGHEYVKEAM
CCCCCHHHHHHHHCCCCCCCHHHEEEEEEEEECCCCCCCCCEEEEEEEECCCHHHHHHHH
DNGAISSLWKKSYGTPPKGIPIIFVDDTLFALQQLAQFYRREINVKVIGITGSNGKTTVK
CCCHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHH
DIIEGILRSTYRVHKTKGNFNSQIGLPLTILEMKRDTEFLILEMGMSEKGQIRNLSRIAQ
HHHHHHHHHHHEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHCC
PDVAVITMIGQSHLETLGSRKEIAKAKLEIIDGLRENGLFLYNGDEPLFSRNNKTSGVNC
CCEEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCE
KTFGEKDTNDLFPTNVQLDEYGVHFKLNHTKIQYDVPLHGKHNIFNTIVGIAVGQFYKVP
EECCCCCCCCCCCCCEEEECCCEEEEEECEEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
TEKIQEALQQINITQMRFQFLTAKIGFTIINDAWNASPSSMKAAIETLQKLNAYKKKIIV
HHHHHHHHHHCCHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEE
IGDMLELGKKAETYHREIGKMLNEESIQYVFTYGELAKIVAEEASKKYDTGKVQSFDNKA
EECHHHHCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCHH
EIAEEVLKVITKKDVVLLKGSRGMALEEIVQNWM
HHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]