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The map label for this gene is thyA [H]

Identifier: 52143217

GI number: 52143217

Start: 2142898

End: 2143854

Strand: Direct

Name: thyA [H]

Synonym: BCZK2020

Alternate gene names: 52143217

Gene position: 2142898-2143854 (Clockwise)

Preceding gene: 52143218

Following gene: 52143216

Centisome position: 40.43

GC content: 37.1

Gene sequence:

>957_bases
ATGAAACATGCTGAAAATGAATACTTAAATTTATGCCGCCATGTAATGGAACATGGTACGAAGAAAGAAGATCGTACAGG
GACAGGAACTGTATCTGTATTTGGATATCAAATGCGTTTTGATTTAAGTAAAGGTTTTCCTTTATTAACGACAAAGAGAG
TACCGTTTCGCCTTGTAGCAAGTGAATTGCTATGGTTTATGAAAGGTGATACAAATATTCGCTATTTATTGCAGCATAAT
AATAACATTTGGAATGAATGGGCATTTAAGAGCTGGGTAGAAAGCGACGAGTATACTGGTCCTGACATGACTGATTTCGG
TCTTCGTTCACAACAAGATGAGGAATTTAAAGTGCAGTACGATAAGCAAATGGAATTATTTAAAAAGAACGTTTTAGAAG
ATGATGATTTCTCAAATAAATATGGTTATTTAGGAGACGTATACGGTAAGCAGTGGCGTGCTTGGAAAACGACAGCTGGT
GAGACACTTGATCAATTAAAGGATGTAATTGAAATGATTAAGAAGACGCCAGATTCGCGTCGCCTAATTGTTTCTGCTTG
GAATCCGGAAGATGTACCAAGTATGGCATTACCGCCTTGTCATACACTATTCCAATTTTATGTAGCAGATGGCAAACTTT
CTTGTCAGCTATATCAAAGAAGTGGTGACATATTCCTTGGAATTCCATTTAACATTGCAAGTTACTCACTACTGACACAT
TTAATTGCACATGAATGCGGACTTGAAGTGGGAGAATTTGTTCATACAATTGGAGATGCACACATTTATACAAACCATTT
TGAGCAAGTAGAAAAGCAATTGGCACGTGAACCACGTCCATTCCCGAAACTTACACTAAATCCAGATGTGAAATCTGTGT
TTGATTTTGAAATGGAAGATTTAACAATTGAAGGATATGATCCGCATCCAGCAATTAAAGCACCAGTTGCAGTGTAA

Upstream 100 bases:

>100_bases
AGAAATGTTTTTTTATAAATTTAGTTTGTTCAATCTGTTAGCATTAAATTTATGATAAAATCCATATAGACTAGAGATTT
GAAAGAAGGTTTTACTACAT

Downstream 100 bases:

>100_bases
TTGTAGAGGAGATGAAAAGATGATAGTTTCATTTATGGTCGCAATGGACGAAAATAGAGTAATTGGTAAAGATAATAATT
TACCTTGGCGTTTACCGAGT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKHAENEYLNLCRHVMEHGTKKEDRTGTGTVSVFGYQMRFDLSKGFPLLTTKRVPFRLVASELLWFMKGDTNIRYLLQHN
NNIWNEWAFKSWVESDEYTGPDMTDFGLRSQQDEEFKVQYDKQMELFKKNVLEDDDFSNKYGYLGDVYGKQWRAWKTTAG
ETLDQLKDVIEMIKKTPDSRRLIVSAWNPEDVPSMALPPCHTLFQFYVADGKLSCQLYQRSGDIFLGIPFNIASYSLLTH
LIAHECGLEVGEFVHTIGDAHIYTNHFEQVEKQLAREPRPFPKLTLNPDVKSVFDFEMEDLTIEGYDPHPAIKAPVAV

Sequences:

>Translated_318_residues
MKHAENEYLNLCRHVMEHGTKKEDRTGTGTVSVFGYQMRFDLSKGFPLLTTKRVPFRLVASELLWFMKGDTNIRYLLQHN
NNIWNEWAFKSWVESDEYTGPDMTDFGLRSQQDEEFKVQYDKQMELFKKNVLEDDDFSNKYGYLGDVYGKQWRAWKTTAG
ETLDQLKDVIEMIKKTPDSRRLIVSAWNPEDVPSMALPPCHTLFQFYVADGKLSCQLYQRSGDIFLGIPFNIASYSLLTH
LIAHECGLEVGEFVHTIGDAHIYTNHFEQVEKQLAREPRPFPKLTLNPDVKSVFDFEMEDLTIEGYDPHPAIKAPVAV
>Mature_318_residues
MKHAENEYLNLCRHVMEHGTKKEDRTGTGTVSVFGYQMRFDLSKGFPLLTTKRVPFRLVASELLWFMKGDTNIRYLLQHN
NNIWNEWAFKSWVESDEYTGPDMTDFGLRSQQDEEFKVQYDKQMELFKKNVLEDDDFSNKYGYLGDVYGKQWRAWKTTAG
ETLDQLKDVIEMIKKTPDSRRLIVSAWNPEDVPSMALPPCHTLFQFYVADGKLSCQLYQRSGDIFLGIPFNIASYSLLTH
LIAHECGLEVGEFVHTIGDAHIYTNHFEQVEKQLAREPRPFPKLTLNPDVKSVFDFEMEDLTIEGYDPHPAIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=324, Percent_Identity=47.2222222222222, Blast_Score=298, Evalue=4e-81,
Organism=Escherichia coli, GI1789191, Length=312, Percent_Identity=51.2820512820513, Blast_Score=323, Evalue=7e-90,
Organism=Caenorhabditis elegans, GI71993377, Length=327, Percent_Identity=42.5076452599388, Blast_Score=272, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6324648, Length=196, Percent_Identity=51.530612244898, Blast_Score=200, Evalue=3e-52,
Organism=Drosophila melanogaster, GI17137556, Length=324, Percent_Identity=46.2962962962963, Blast_Score=295, Evalue=2e-80,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 36866; Mature: 36866

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHAENEYLNLCRHVMEHGTKKEDRTGTGTVSVFGYQMRFDLSKGFPLLTTKRVPFRLVA
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEECCCCHHHHH
SELLWFMKGDTNIRYLLQHNNNIWNEWAFKSWVESDEYTGPDMTDFGLRSQQDEEFKVQY
HHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
DKQMELFKKNVLEDDDFSNKYGYLGDVYGKQWRAWKTTAGETLDQLKDVIEMIKKTPDSR
HHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCHHHHHCHHHHHHHHHHHHHHHHHCCCCCC
RLIVSAWNPEDVPSMALPPCHTLFQFYVADGKLSCQLYQRSGDIFLGIPFNIASYSLLTH
EEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHHHHHHHH
LIAHECGLEVGEFVHTIGDAHIYTNHFEQVEKQLAREPRPFPKLTLNPDVKSVFDFEMED
HHHHHHCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHCCCHHC
LTIEGYDPHPAIKAPVAV
EEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKHAENEYLNLCRHVMEHGTKKEDRTGTGTVSVFGYQMRFDLSKGFPLLTTKRVPFRLVA
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEECCCCHHHHH
SELLWFMKGDTNIRYLLQHNNNIWNEWAFKSWVESDEYTGPDMTDFGLRSQQDEEFKVQY
HHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
DKQMELFKKNVLEDDDFSNKYGYLGDVYGKQWRAWKTTAGETLDQLKDVIEMIKKTPDSR
HHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCHHHHHCHHHHHHHHHHHHHHHHHCCCCCC
RLIVSAWNPEDVPSMALPPCHTLFQFYVADGKLSCQLYQRSGDIFLGIPFNIASYSLLTH
EEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHHHHHHHH
LIAHECGLEVGEFVHTIGDAHIYTNHFEQVEKQLAREPRPFPKLTLNPDVKSVFDFEMED
HHHHHHCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHCCCHHC
LTIEGYDPHPAIKAPVAV
EEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA