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The map label for this gene is dfrA [H]

Identifier: 52143216

GI number: 52143216

Start: 2143874

End: 2144362

Strand: Direct

Name: dfrA [H]

Synonym: BCZK2021

Alternate gene names: 52143216

Gene position: 2143874-2144362 (Clockwise)

Preceding gene: 52143217

Following gene: 52143215

Centisome position: 40.44

GC content: 33.74

Gene sequence:

>489_bases
ATGATAGTTTCATTTATGGTCGCAATGGACGAAAATAGAGTAATTGGTAAAGATAATAATTTACCTTGGCGTTTACCGAG
TGAATTACAGTATGTAAAGAAAACAACGATGGGTCACCCGCTTATTATGGGAAGAAAAAACTATGAAGCGATTGGTAGAC
CACTGCCTGGAAGACGTAATATTATTGTAACTCGCAATGAAGGATATCATGTTGAAGGCTGTGAAGTAGCACATTCTGTA
GAAGAAGTGTTTGAGCTATGCAAAAATGAAGAAGAGATCTTTATTTTTGGCGGAGCACAAATTTATGATCTCTTTTTACC
TTACGTAGACAAGTTATATATAACAAAAATCCATCATGCATTTGAAGGAGATACATTCTTCCCAGAAATGGATATGACAA
ATTGGAAAGAAGTTTTTGTTGAAAAAGGTTTAACGGATGAAAAAAATCCGTATACGTATTATTACCATGTATATGAGAAA
CAACAATAA

Upstream 100 bases:

>100_bases
GTGTTTGATTTTGAAATGGAAGATTTAACAATTGAAGGATATGATCCGCATCCAGCAATTAAAGCACCAGTTGCAGTGTA
ATTGTAGAGGAGATGAAAAG

Downstream 100 bases:

>100_bases
TCCCAAAGATTGATGTGAATATTCGCATCAATCTTTTTATTTGTATCCATTAATATTGGTTTATAGATGAGATTCGATAG
TGCTATAATGAAAGGTAGCA

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSV
EEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEK
QQ

Sequences:

>Translated_162_residues
MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSV
EEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEK
QQ
>Mature_162_residues
MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSV
EEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEK
QQ

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=178, Percent_Identity=33.1460674157303, Blast_Score=77, Evalue=6e-15,
Organism=Homo sapiens, GI28827793, Length=156, Percent_Identity=30.7692307692308, Blast_Score=68, Evalue=4e-12,
Organism=Escherichia coli, GI1786233, Length=160, Percent_Identity=39.375, Blast_Score=140, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17505899, Length=155, Percent_Identity=32.9032258064516, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324810, Length=166, Percent_Identity=31.3253012048193, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI116008026, Length=177, Percent_Identity=32.7683615819209, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24647458, Length=177, Percent_Identity=32.7683615819209, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19125; Mature: 19125

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRN
CEEEEEEEECCCEEEECCCCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHCCCCCCCCC
IIVTRNEGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHA
EEEECCCCCEECHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
FEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEKQQ
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEEECCC
>Mature Secondary Structure
MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRN
CEEEEEEEECCCEEEECCCCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHCCCCCCCCC
IIVTRNEGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHA
EEEECCCCCEECHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
FEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEKQQ
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8540692 [H]