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The map label for this gene is rocR [H]

Identifier: 52143161

GI number: 52143161

Start: 2201807

End: 2203192

Strand: Direct

Name: rocR [H]

Synonym: BCZK2078

Alternate gene names: 52143161

Gene position: 2201807-2203192 (Clockwise)

Preceding gene: 52143160

Following gene: 52143159

Centisome position: 41.54

GC content: 36.44

Gene sequence:

>1386_bases
ATGACATTGCTAGCAGTTTCGACACAAGAAGTCATTGAAGCGATTTTGGGCAGTATTGATGAGGCTATACACGCAGTAGA
TGAAAATGGTATTACAATTTTCTATAATACTGTAGCTGCAAAACACGATGGATCAAAAATTGAAAAAGTGTTAGGTAAAC
ATTTGTTAGAAGCGTTCCCTTCTTTATCTAGGGAAACAAGTACGTTGATGAAAGTGTTAGATACAAAAAAACCAATCGTT
CATCAAGTGCAGCATTATCAAAATTTAAATGGAGAAGACATTTGCACAGTAAATACGACGTTACCTATTCTTATAGATGG
AAACATTGCTGGTGCTGTTGAAATTGCAAAGGATTATTCCACAGTTCAAAAACTTACGGATACAATAGTCGATTTGCAGT
CGAAAATAAAGCGGTCATCTAGAAAAAAAGCGATCAAAAAGCATGTTGCATTTGAGACAATCGTGACGAATGACGCTCGG
TTTAAACAGACGAAAGAATTAGCACAAAAAGTAGCTCCAACTGATGCGAATGTTTTAATATATGGGGAAACAGGAACAGG
GAAAGAGTTATTTGTACAAGCGATTCATGAAGCTTCTAAACGGAAAAACAAGCCGTTTATTGCACAAAACTGCGCAGCTT
TGCCAGAGTCTCTATTAGAAAGTTTATTGTTTGGAACGACGAAAGGTAGTTATACTGGGGCAATTGAACGAGCGGGATTA
TTTGAACTTGTAGATGGAGGGACATTGTTTTTAGATGAACTGAATTCAATGCCACTCGATTTACAAGCGAAAATGCTACG
TGTATTAGAAGATGGTGTTATTCGTCGTATTGGTGATAATAAGACGAGAAAAGTAGATGTTCGCGTTATTACGGCAATGA
ATCAGCCACCAGAAGTATGTTTAAAAGAGAATAAAATTCGCACTGATTTATACTATCGATTAAATGTCTTTTCATTATAT
ATTCCGCCGCTCCGTGAAAGAACGGAGGATGTATTATTATTAGCGTCTTATTTTTTGAAAGAATATAATAAAAGTTATAA
AAAAGGTGTACTTCAAATGGATCAGGAAGCGAAAGAAAGATTACAATCTTACCAATGGCCTGGAAATGTTCGGGAGCTAA
AACATACGATTGAACATGCTGTCATTATCGCAGAAGGGAATACATTAACAGCCAATTGTTTACCGCGTACATTTCGGAAA
GAGAGTTTTCCGAAGAAGAAGAACATATTGCCGCTTAGAGAAGCACTTCATCAAACAGAAAAAGAATTAATAGATCAAGC
GTTAATTGAAACGGAAGGGAATATACTACAAGCTGCAAAAATGTTAGGCATCCCTCGTCAAACGCTTCAATATAAACTGA
GTAAGTACGACAAAACCGCCGAATAA

Upstream 100 bases:

>100_bases
GTACGTTATAAAAATAGAACGGAAACGTCATGCTTATAATCGCACATAGGTATGCCGGAAATTCGGCATAGTATTTGCTG
AATTTTCGGCAAGAGGGGGG

Downstream 100 bases:

>100_bases
TTGGCGGTTTTTGTGTGTTTGCACGAATAAAAGAGCGTTTACATATATGTAAAGTAATGAATATGATAGTATGTATGAAT
TTCATACAGTGCTTAAAAAA

Product: arginine utilization regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MTLLAVSTQEVIEAILGSIDEAIHAVDENGITIFYNTVAAKHDGSKIEKVLGKHLLEAFPSLSRETSTLMKVLDTKKPIV
HQVQHYQNLNGEDICTVNTTLPILIDGNIAGAVEIAKDYSTVQKLTDTIVDLQSKIKRSSRKKAIKKHVAFETIVTNDAR
FKQTKELAQKVAPTDANVLIYGETGTGKELFVQAIHEASKRKNKPFIAQNCAALPESLLESLLFGTTKGSYTGAIERAGL
FELVDGGTLFLDELNSMPLDLQAKMLRVLEDGVIRRIGDNKTRKVDVRVITAMNQPPEVCLKENKIRTDLYYRLNVFSLY
IPPLRERTEDVLLLASYFLKEYNKSYKKGVLQMDQEAKERLQSYQWPGNVRELKHTIEHAVIIAEGNTLTANCLPRTFRK
ESFPKKKNILPLREALHQTEKELIDQALIETEGNILQAAKMLGIPRQTLQYKLSKYDKTAE

Sequences:

>Translated_461_residues
MTLLAVSTQEVIEAILGSIDEAIHAVDENGITIFYNTVAAKHDGSKIEKVLGKHLLEAFPSLSRETSTLMKVLDTKKPIV
HQVQHYQNLNGEDICTVNTTLPILIDGNIAGAVEIAKDYSTVQKLTDTIVDLQSKIKRSSRKKAIKKHVAFETIVTNDAR
FKQTKELAQKVAPTDANVLIYGETGTGKELFVQAIHEASKRKNKPFIAQNCAALPESLLESLLFGTTKGSYTGAIERAGL
FELVDGGTLFLDELNSMPLDLQAKMLRVLEDGVIRRIGDNKTRKVDVRVITAMNQPPEVCLKENKIRTDLYYRLNVFSLY
IPPLRERTEDVLLLASYFLKEYNKSYKKGVLQMDQEAKERLQSYQWPGNVRELKHTIEHAVIIAEGNTLTANCLPRTFRK
ESFPKKKNILPLREALHQTEKELIDQALIETEGNILQAAKMLGIPRQTLQYKLSKYDKTAE
>Mature_460_residues
TLLAVSTQEVIEAILGSIDEAIHAVDENGITIFYNTVAAKHDGSKIEKVLGKHLLEAFPSLSRETSTLMKVLDTKKPIVH
QVQHYQNLNGEDICTVNTTLPILIDGNIAGAVEIAKDYSTVQKLTDTIVDLQSKIKRSSRKKAIKKHVAFETIVTNDARF
KQTKELAQKVAPTDANVLIYGETGTGKELFVQAIHEASKRKNKPFIAQNCAALPESLLESLLFGTTKGSYTGAIERAGLF
ELVDGGTLFLDELNSMPLDLQAKMLRVLEDGVIRRIGDNKTRKVDVRVITAMNQPPEVCLKENKIRTDLYYRLNVFSLYI
PPLRERTEDVLLLASYFLKEYNKSYKKGVLQMDQEAKERLQSYQWPGNVRELKHTIEHAVIIAEGNTLTANCLPRTFRKE
SFPKKKNILPLREALHQTEKELIDQALIETEGNILQAAKMLGIPRQTLQYKLSKYDKTAE

Specific function: Positive regulator of arginine catabolism. Controls the transcription of the two operons rocABC and rocDEF and probably acts by binding to the corresponding upstream activating sequences [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=342, Percent_Identity=43.2748538011696, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI87082117, Length=439, Percent_Identity=35.5353075170843, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1788905, Length=311, Percent_Identity=42.7652733118971, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1790437, Length=288, Percent_Identity=44.0972222222222, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI87082152, Length=294, Percent_Identity=45.2380952380952, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1789087, Length=371, Percent_Identity=38.2749326145553, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=39.8753894080997, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1790299, Length=308, Percent_Identity=40.2597402597403, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1786524, Length=328, Percent_Identity=37.5, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI87081872, Length=311, Percent_Identity=38.2636655948553, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1787583, Length=275, Percent_Identity=38.5454545454545, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87081858, Length=309, Percent_Identity=31.0679611650485, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI1789828, Length=284, Percent_Identity=30.2816901408451, Blast_Score=114, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF08448 PAS_4; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51847; Mature: 51715

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLAVSTQEVIEAILGSIDEAIHAVDENGITIFYNTVAAKHDGSKIEKVLGKHLLEAFP
CEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
SLSRETSTLMKVLDTKKPIVHQVQHYQNLNGEDICTVNTTLPILIDGNIAGAVEIAKDYS
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECEEEEEECCCCCCHHHHHHHHH
TVQKLTDTIVDLQSKIKRSSRKKAIKKHVAFETIVTNDARFKQTKELAQKVAPTDANVLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEE
YGETGTGKELFVQAIHEASKRKNKPFIAQNCAALPESLLESLLFGTTKGSYTGAIERAGL
EECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCH
FELVDGGTLFLDELNSMPLDLQAKMLRVLEDGVIRRIGDNKTRKVDVRVITAMNQPPEVC
HEEECCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHH
LKENKIRTDLYYRLNVFSLYIPPLRERTEDVLLLASYFLKEYNKSYKKGVLQMDQEAKER
HCCCCCCCHHEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQSYQWPGNVRELKHTIEHAVIIAEGNTLTANCLPRTFRKESFPKKKNILPLREALHQTE
HHHCCCCCCHHHHHHHHHHEEEEECCCEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHH
KELIDQALIETEGNILQAAKMLGIPRQTLQYKLSKYDKTAE
HHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TLLAVSTQEVIEAILGSIDEAIHAVDENGITIFYNTVAAKHDGSKIEKVLGKHLLEAFP
EEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
SLSRETSTLMKVLDTKKPIVHQVQHYQNLNGEDICTVNTTLPILIDGNIAGAVEIAKDYS
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECEEEEEECCCCCCHHHHHHHHH
TVQKLTDTIVDLQSKIKRSSRKKAIKKHVAFETIVTNDARFKQTKELAQKVAPTDANVLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEE
YGETGTGKELFVQAIHEASKRKNKPFIAQNCAALPESLLESLLFGTTKGSYTGAIERAGL
EECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCH
FELVDGGTLFLDELNSMPLDLQAKMLRVLEDGVIRRIGDNKTRKVDVRVITAMNQPPEVC
HEEECCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHH
LKENKIRTDLYYRLNVFSLYIPPLRERTEDVLLLASYFLKEYNKSYKKGVLQMDQEAKER
HCCCCCCCHHEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQSYQWPGNVRELKHTIEHAVIIAEGNTLTANCLPRTFRKESFPKKKNILPLREALHQTE
HHHCCCCCCHHHHHHHHHHEEEEECCCEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHH
KELIDQALIETEGNILQAAKMLGIPRQTLQYKLSKYDKTAE
HHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113162; 9384377 [H]