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The map label for this gene is dacA [H]

Identifier: 52142940

GI number: 52142940

Start: 2435219

End: 2436235

Strand: Reverse

Name: dacA [H]

Synonym: BCZK2300

Alternate gene names: 52142940

Gene position: 2436235-2435219 (Counterclockwise)

Preceding gene: 52142939

Following gene: 52142945

Centisome position: 45.96

GC content: 30.88

Gene sequence:

>1017_bases
ATGCAATTCTTAAAAAAATTCGCTATTTTATTATTATCTTTTTTTATTACCGCACTTATCGTTTTATATTTTTACCTTTA
TGTAAATGGCCCAATTATTCAAGCTAAATCAGCTATTTTAATAAATGCTCATTCTGGTGAAGTTGTTTATAAAAAAAATG
AAGAAACTCCTATACAATCAGCTACTTTATCTAAATTAATGACTGAATATATCACACTGGAACAACTAAATGAAGGAACT
ATACAGTTAGATGAAGTAGTAAAAATAAGTAACGAAGTTTTCCGAGCAGAAACGAGTCCTATACAAGTAATTTCGAAAGA
TAAAACGACGGTTCGTGATTTACTTCATACACTGTTATTGACAGGAAATAATCGTTCCGCACTTGCTCTTGCAGAACACA
TCGCTGGAAACGAAGATAAATTCACACAGTTAATGAATGAAAAAGCAAAACAACTAAAGTTATCACAATTATCTCCGTTT
CTAAACGCTACTGGAATGAATAACGATACAAATAAACTTTCAACTACAACAGCAATTGAAGTTGCTAAACTAGCAACACA
ATTAGTAACAGACTTTCCAGATGTACTGAACGTTACAAAACTAACCTCTTACCAGTTCACCTTCAAAGATACCAAAGTTT
TCAATACCAATAAAATGATTTATTCCCTAAATAAAAACATTAAACTCCAAGGTGTTGATGGCTTACAAACTAGTTTTTCA
ACTAATAGTAATTATAGTTTTGTCAGTACAGCAAAACGAGGAGATACTAGACTTATTTCAGTAATATTAGACGCCGATAA
TGAAAACAGTACTTTTATTGAAACAAAAAAATTACTACAGTACGGCTTTGATCCCTCCTCGTACTCTGCACTCCAAGCAT
TTAAAGATGCTGTTACATCTTGGACAGTTTTGCTTCAGTTTAAAAATTTGATCATACAAACATTGTTGATTTTCTTTATT
ATTACAATTTTAATGCTTTTACATATACGTCAGAAAAAATCCGAGGATTTCAACTAG

Upstream 100 bases:

>100_bases
TCCTTTATAATTCTTTAGAAATTCTTTACCTTTTCTTTCTTCCTAATTAAGCATTCCTTTTTACAATATTATTATCGACG
TCATAATAGGAGGATCGCTT

Downstream 100 bases:

>100_bases
GAAATCTTCTTATATATAAAGAACGGCTTGCATTTACAATGCAAGCCGTTTTCTAAATTTATAGTCCTCTCCCAATTAAA
TTTGCTGAAACAGACCGAAG

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MQFLKKFAILLLSFFITALIVLYFYLYVNGPIIQAKSAILINAHSGEVVYKKNEETPIQSATLSKLMTEYITLEQLNEGT
IQLDEVVKISNEVFRAETSPIQVISKDKTTVRDLLHTLLLTGNNRSALALAEHIAGNEDKFTQLMNEKAKQLKLSQLSPF
LNATGMNNDTNKLSTTTAIEVAKLATQLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKNIKLQGVDGLQTSFS
TNSNYSFVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFDPSSYSALQAFKDAVTSWTVLLQFKNLIIQTLLIFFI
ITILMLLHIRQKKSEDFN

Sequences:

>Translated_338_residues
MQFLKKFAILLLSFFITALIVLYFYLYVNGPIIQAKSAILINAHSGEVVYKKNEETPIQSATLSKLMTEYITLEQLNEGT
IQLDEVVKISNEVFRAETSPIQVISKDKTTVRDLLHTLLLTGNNRSALALAEHIAGNEDKFTQLMNEKAKQLKLSQLSPF
LNATGMNNDTNKLSTTTAIEVAKLATQLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKNIKLQGVDGLQTSFS
TNSNYSFVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFDPSSYSALQAFKDAVTSWTVLLQFKNLIIQTLLIFFI
ITILMLLHIRQKKSEDFN
>Mature_338_residues
MQFLKKFAILLLSFFITALIVLYFYLYVNGPIIQAKSAILINAHSGEVVYKKNEETPIQSATLSKLMTEYITLEQLNEGT
IQLDEVVKISNEVFRAETSPIQVISKDKTTVRDLLHTLLLTGNNRSALALAEHIAGNEDKFTQLMNEKAKQLKLSQLSPF
LNATGMNNDTNKLSTTTAIEVAKLATQLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKNIKLQGVDGLQTSFS
TNSNYSFVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFDPSSYSALQAFKDAVTSWTVLLQFKNLIIQTLLIFFI
ITILMLLHIRQKKSEDFN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=262, Percent_Identity=30.1526717557252, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87082022, Length=259, Percent_Identity=27.027027027027, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786851, Length=262, Percent_Identity=28.6259541984733, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87082054, Length=257, Percent_Identity=24.9027237354086, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 38118; Mature: 38118

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFLKKFAILLLSFFITALIVLYFYLYVNGPIIQAKSAILINAHSGEVVYKKNEETPIQS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCEEEECCCCCCHHH
ATLSKLMTEYITLEQLNEGTIQLDEVVKISNEVFRAETSPIQVISKDKTTVRDLLHTLLL
HHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHH
TGNNRSALALAEHIAGNEDKFTQLMNEKAKQLKLSQLSPFLNATGMNNDTNKLSTTTAIE
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHH
VAKLATQLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKNIKLQGVDGLQTSFS
HHHHHHHHHHCCHHHHHHHHHEEEEEEECCCEEEECCCEEEEECCCEEEEECCCCHHCCC
TNSNYSFVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFDPSSYSALQAFKDAVTS
CCCCCEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
WTVLLQFKNLIIQTLLIFFIITILMLLHIRQKKSEDFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQFLKKFAILLLSFFITALIVLYFYLYVNGPIIQAKSAILINAHSGEVVYKKNEETPIQS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCEEEECCCCCCHHH
ATLSKLMTEYITLEQLNEGTIQLDEVVKISNEVFRAETSPIQVISKDKTTVRDLLHTLLL
HHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHH
TGNNRSALALAEHIAGNEDKFTQLMNEKAKQLKLSQLSPFLNATGMNNDTNKLSTTTAIE
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHH
VAKLATQLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKNIKLQGVDGLQTSFS
HHHHHHHHHHCCHHHHHHHHHEEEEEEECCCEEEECCCEEEEECCCEEEEECCCCHHCCC
TNSNYSFVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFDPSSYSALQAFKDAVTS
CCCCCEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
WTVLLQFKNLIIQTLLIFFIITILMLLHIRQKKSEDFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]