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The map label for this gene is acoL [H]

Identifier: 52142738

GI number: 52142738

Start: 2632806

End: 2634185

Strand: Reverse

Name: acoL [H]

Synonym: BCZK2502

Alternate gene names: 52142738

Gene position: 2634185-2632806 (Counterclockwise)

Preceding gene: 52142737

Following gene: 52142740

Centisome position: 49.69

GC content: 37.9

Gene sequence:

>1380_bases
ATGAGTAAATTAGTCGTTATTGGAGGCGGACCTGCTGGCTATGTAGCGGCAATTACCGCGGCTCAAAATGGAAAAAATGT
CACTCTTATTGATGAAGCTGATCTTGGCGGGACTTGTTTAAATGTCGGGTGCATGCCTACAAAATCATTGTTAGAAAGTG
CAGAAGTGCATGACATTGTGAGAAAATCGAATCATTATGGAGTTACGCTTAACAATGGAAGTATATCAATCGATTGGAAG
CAAATGCAGGTGAGAAAATCGCAAATCGTAACGCAGCTCGTCCAAGGAATTCAATATTTAATGAAGAAAAATAAAATAAA
AGTTATACAAGGTAAAGCGAAATTTGAAACGGATCATCGTGTGAGAGTTACATACGGTGATAAAGAAATTGTAGTAGATG
GAGAACAGTTCATTATAGCGACAGGTTCAGAACCGACTGAATTACCTTTTGCTCCATTTGACGGGAAGTGGATTTTAAAT
AGTACCCATGCAATGTCTCTAAAAAACATACCGAAATCATTATTAATCGTTGGCGGTGGTGTAATAGGGTGCGAGTTTGC
AAGCATTTATAGTCGTCTTGGAACAAAAGTTACAATTGTTGAAATGGCACCGCAACTATTACCAGGTGAAGATGAAGATA
TTGCACACATTTTAAGAAGGGAACTAGAAAATGATGGCGTAAAAATATTTACAGGAGCAACTTTAAAAGGATTAAATAGT
TATAAGAAGCAGGCTTTATTTGAATATGAAGGAAGTATCCAAGAAGTTAATACAGAATTTGTTCTCGTTTCTGTCGGCCG
AAAACCACGTGTACAACAATTAAATTTAGAAAAAGCAGGCATTCAATATTCAAATAAAGGAATTTCTGTGAATGAACATA
TGCAAACCAATGTATCACATATTTACGCAGCAGGTGATGTGATTGGTGGAATCCAGCTTGCTCACGTTGCATTCTATGAA
GGAACGACGGCAGCATTACACGCGAGTGGAGAAGATGTAAAAGTAAACTACCATGCTGTACCTCGCTGTATTTATACAGC
TCCAGAAATTGCCAGTGTCGGTTTAACAGAAAAAGATGCAAGAGAGCAGTATGGTGACATACAAATTGGAGAATTTCCTT
TTACAGCGAACGGAAAAGCGCTTATTATTGGCGAACAAATGGGTAAAGTAAAAGTTATTGTGGAACCTAAATATCAAGAA
ATTGTAGGGATTTCTATTATTGGTCCACGTGCAACTGAACTGATTGGACAAGGAACTGTAATGATTCATACAGAAGTTAC
CGCTGATATTATGAGAGATTATATTGCGGCACACCCAACTTTATCTGAAGCAATTCATGAAGCGCTATTGCAAGCGGTAG
GACATGCTGTACATGCTTAA

Upstream 100 bases:

>100_bases
TCGTGTACTAGACGGTGCACCAGCAGCTGCATTTTTACGCACAATTAAACGCTATTTAGAAGAACCTGTAACAATTCTTT
TATAAAGAGAAGGTGAGAGA

Downstream 100 bases:

>100_bases
ATGGAATTGTAATAAACCACTATTCTCTAAATGTATGAGAGAATAGTGGTTTGAATTTTTATAGAAGAAATTAAATATCT
TATTACTATTTAATTTCAAT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of acetoin cleaving system [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSKLVVIGGGPAGYVAAITAAQNGKNVTLIDEADLGGTCLNVGCMPTKSLLESAEVHDIVRKSNHYGVTLNNGSISIDWK
QMQVRKSQIVTQLVQGIQYLMKKNKIKVIQGKAKFETDHRVRVTYGDKEIVVDGEQFIIATGSEPTELPFAPFDGKWILN
STHAMSLKNIPKSLLIVGGGVIGCEFASIYSRLGTKVTIVEMAPQLLPGEDEDIAHILRRELENDGVKIFTGATLKGLNS
YKKQALFEYEGSIQEVNTEFVLVSVGRKPRVQQLNLEKAGIQYSNKGISVNEHMQTNVSHIYAAGDVIGGIQLAHVAFYE
GTTAALHASGEDVKVNYHAVPRCIYTAPEIASVGLTEKDAREQYGDIQIGEFPFTANGKALIIGEQMGKVKVIVEPKYQE
IVGISIIGPRATELIGQGTVMIHTEVTADIMRDYIAAHPTLSEAIHEALLQAVGHAVHA

Sequences:

>Translated_459_residues
MSKLVVIGGGPAGYVAAITAAQNGKNVTLIDEADLGGTCLNVGCMPTKSLLESAEVHDIVRKSNHYGVTLNNGSISIDWK
QMQVRKSQIVTQLVQGIQYLMKKNKIKVIQGKAKFETDHRVRVTYGDKEIVVDGEQFIIATGSEPTELPFAPFDGKWILN
STHAMSLKNIPKSLLIVGGGVIGCEFASIYSRLGTKVTIVEMAPQLLPGEDEDIAHILRRELENDGVKIFTGATLKGLNS
YKKQALFEYEGSIQEVNTEFVLVSVGRKPRVQQLNLEKAGIQYSNKGISVNEHMQTNVSHIYAAGDVIGGIQLAHVAFYE
GTTAALHASGEDVKVNYHAVPRCIYTAPEIASVGLTEKDAREQYGDIQIGEFPFTANGKALIIGEQMGKVKVIVEPKYQE
IVGISIIGPRATELIGQGTVMIHTEVTADIMRDYIAAHPTLSEAIHEALLQAVGHAVHA
>Mature_458_residues
SKLVVIGGGPAGYVAAITAAQNGKNVTLIDEADLGGTCLNVGCMPTKSLLESAEVHDIVRKSNHYGVTLNNGSISIDWKQ
MQVRKSQIVTQLVQGIQYLMKKNKIKVIQGKAKFETDHRVRVTYGDKEIVVDGEQFIIATGSEPTELPFAPFDGKWILNS
THAMSLKNIPKSLLIVGGGVIGCEFASIYSRLGTKVTIVEMAPQLLPGEDEDIAHILRRELENDGVKIFTGATLKGLNSY
KKQALFEYEGSIQEVNTEFVLVSVGRKPRVQQLNLEKAGIQYSNKGISVNEHMQTNVSHIYAAGDVIGGIQLAHVAFYEG
TTAALHASGEDVKVNYHAVPRCIYTAPEIASVGLTEKDAREQYGDIQIGEFPFTANGKALIIGEQMGKVKVIVEPKYQEI
VGISIIGPRATELIGQGTVMIHTEVTADIMRDYIAAHPTLSEAIHEALLQAVGHAVHA

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=460, Percent_Identity=35.8695652173913, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI50301238, Length=448, Percent_Identity=29.0178571428571, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI148277065, Length=461, Percent_Identity=27.3318872017354, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI33519430, Length=461, Percent_Identity=27.3318872017354, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI33519428, Length=461, Percent_Identity=27.3318872017354, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI33519426, Length=461, Percent_Identity=27.3318872017354, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI148277071, Length=461, Percent_Identity=27.3318872017354, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI22035672, Length=458, Percent_Identity=28.3842794759825, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI291045266, Length=456, Percent_Identity=26.5350877192982, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI291045268, Length=451, Percent_Identity=24.6119733924612, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1786307, Length=449, Percent_Identity=32.9621380846325, Blast_Score=261, Evalue=6e-71,
Organism=Escherichia coli, GI87081717, Length=451, Percent_Identity=31.9290465631929, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI87082354, Length=456, Percent_Identity=27.6315789473684, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789915, Length=433, Percent_Identity=28.4064665127021, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI32565766, Length=466, Percent_Identity=37.5536480686695, Blast_Score=278, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI17557007, Length=470, Percent_Identity=27.4468085106383, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71983429, Length=442, Percent_Identity=26.2443438914027, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71983419, Length=442, Percent_Identity=26.2443438914027, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=457, Percent_Identity=25.601750547046, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17559934, Length=224, Percent_Identity=29.0178571428571, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=472, Percent_Identity=38.3474576271186, Blast_Score=280, Evalue=4e-76,
Organism=Saccharomyces cerevisiae, GI6325166, Length=449, Percent_Identity=27.1714922048998, Blast_Score=160, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6325240, Length=480, Percent_Identity=26.4583333333333, Blast_Score=159, Evalue=7e-40,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=37.2549019607843, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24640553, Length=465, Percent_Identity=27.9569892473118, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24640551, Length=465, Percent_Identity=27.9569892473118, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24640549, Length=465, Percent_Identity=27.9569892473118, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI17737741, Length=466, Percent_Identity=27.4678111587983, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49739; Mature: 49608

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLVVIGGGPAGYVAAITAAQNGKNVTLIDEADLGGTCLNVGCMPTKSLLESAEVHDIV
CCEEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHH
RKSNHYGVTLNNGSISIDWKQMQVRKSQIVTQLVQGIQYLMKKNKIKVIQGKAKFETDHR
HCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCE
VRVTYGDKEIVVDGEQFIIATGSEPTELPFAPFDGKWILNSTHAMSLKNIPKSLLIVGGG
EEEEECCEEEEEECCEEEEEECCCCCCCCCCCCCCEEEECCCCEEHHHCCCCEEEEECCC
VIGCEFASIYSRLGTKVTIVEMAPQLLPGEDEDIAHILRRELENDGVKIFTGATLKGLNS
EEEHHHHHHHHHHCCEEEEEECCHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHH
YKKQALFEYEGSIQEVNTEFVLVSVGRKPRVQQLNLEKAGIQYSNKGISVNEHMQTNVSH
HHHHHHHHHCCCCEECCCEEEEEECCCCCCCEEECCHHHCCEECCCCCCCCHHHHCCCCE
IYAAGDVIGGIQLAHVAFYEGTTAALHASGEDVKVNYHAVPRCIYTAPEIASVGLTEKDA
EEECCCHHCCEEEEEEEEECCCEEEEEECCCEEEEEEEECCEEEECCCCHHHCCCCCHHH
REQYGDIQIGEFPFTANGKALIIGEQMGKVKVIVEPKYQEIVGISIIGPRATELIGQGTV
HHHHCCEEECCCCCCCCCCEEEEECCCCCEEEEECCCHHHEEEEEEECCCHHHHHCCCEE
MIHTEVTADIMRDYIAAHPTLSEAIHEALLQAVGHAVHA
EEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKLVVIGGGPAGYVAAITAAQNGKNVTLIDEADLGGTCLNVGCMPTKSLLESAEVHDIV
CEEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHH
RKSNHYGVTLNNGSISIDWKQMQVRKSQIVTQLVQGIQYLMKKNKIKVIQGKAKFETDHR
HCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCE
VRVTYGDKEIVVDGEQFIIATGSEPTELPFAPFDGKWILNSTHAMSLKNIPKSLLIVGGG
EEEEECCEEEEEECCEEEEEECCCCCCCCCCCCCCEEEECCCCEEHHHCCCCEEEEECCC
VIGCEFASIYSRLGTKVTIVEMAPQLLPGEDEDIAHILRRELENDGVKIFTGATLKGLNS
EEEHHHHHHHHHHCCEEEEEECCHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHH
YKKQALFEYEGSIQEVNTEFVLVSVGRKPRVQQLNLEKAGIQYSNKGISVNEHMQTNVSH
HHHHHHHHHCCCCEECCCEEEEEECCCCCCCEEECCHHHCCEECCCCCCCCHHHHCCCCE
IYAAGDVIGGIQLAHVAFYEGTTAALHASGEDVKVNYHAVPRCIYTAPEIASVGLTEKDA
EEECCCHHCCEEEEEEEEECCCEEEEEECCCEEEEEEEECCEEEECCCCHHHCCCCCHHH
REQYGDIQIGEFPFTANGKALIIGEQMGKVKVIVEPKYQEIVGISIIGPRATELIGQGTV
HHHHCCEEECCCCCCCCCCEEEEECCCCCEEEEECCCHHHEEEEEEECCCHHHHHCCCEE
MIHTEVTADIMRDYIAAHPTLSEAIHEALLQAVGHAVHA
EEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10368162; 8969503; 9384377 [H]