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The map label for this gene is acoC [H]

Identifier: 52142737

GI number: 52142737

Start: 2634201

End: 2635397

Strand: Reverse

Name: acoC [H]

Synonym: BCZK2503

Alternate gene names: 52142737

Gene position: 2635397-2634201 (Counterclockwise)

Preceding gene: 52142735

Following gene: 52142738

Centisome position: 49.72

GC content: 35.51

Gene sequence:

>1197_bases
ATGGCTGTAGAAGTTGTAATGCCGAAGTTAGGGATGGCGATGAAAGAAGGTATTATTACGAGCTGGAATATTAAAGCAGG
CGATCATGTATCAAAAGGTGAACTAATTGCTAGTATTAACTCGGAAAAAATTGAAACGGAAATAGAAGCACCAGCTGATG
GAACTGTTCTTGATATAGCTGTAAGTGAAGATGAGGGGGTTCCACCTGGCACTGTAATTTGCTACATTGGTAAACCGAAT
GAAAAAGTAGAGGTACATGAAAGCACAAAGGTTGAGGAAGAACTGGTAGCGAGTGAAGTATCACAAAATGTACAACATCC
AGAACCGTTAGGGAAAGAAGTTACGAATAAACAAAGAATCAAAATATCTCCTGTAGCAAAGAAAATTGCAAAAACTGAAA
ATCTTGATATTAAATCATTACTTGGTACAGGTCCAGGTGGAAGAATTACAAAAGTAGATGTATTAAAGGCACTTGAAGAA
AGAGTAGCAATTCCAGAAGTACTTGAAGAAAGTAAAGTAATTCCTGTTACTGGTATGCGGAAAGCAATCGCAAACCGTAT
GCATGCAAGCTTACAAAATAGTGCGCAATTAACATTAACGATGAAAGTAGATGTTACAGATTTAGTTGCTTTACATAAAG
AAATAGCGAAAGTTGTACAAAAACGATACAATAACAAACTAACCATTACGGATTTCGTTTCTCGCGCTGTTGTATTAGCG
CTTGGAGAGCATAAAGAAATGAATAGCGCTTATATGGATGATACAATTCATCAATTTGAGCATGTTCATTTAGGTATGGC
AATTGCATTAGAAAAAGGATTAGTCGTTCCAGCTATTCGCTTTGCAAATAATCTATCTTTAGTAGAGTTATCTAAACAAA
TTAAAAACGTTGCACAAAAAGCACGAGCAGGCAGTTTAAGTAGTGATGATATGCAAGGAACAACCTTTACAATTAGTAAT
TTAGGTAGCTTTGGGATTGAATATTTTACGCCAGTATTAAATACACCGGAAGCTGGTATTTTAGGTGTAGGTGCAATTGA
ACATGTTCCTGTATATAAAGGAAAAAAATTAAAAAAAGGAAGTATGTTACCTTTAAGTTTAACATTCGATCATCGTGTAC
TAGACGGTGCACCAGCAGCTGCATTTTTACGCACAATTAAACGCTATTTAGAAGAACCTGTAACAATTCTTTTATAA

Upstream 100 bases:

>100_bases
TTTACCAACGCCGGAGAAAGTAATTGAAACTGTATCAGAAATGATCGGAGACCAATCTTTATTACATGTGTAATGAAACG
ATGAGAAGGGAGAGAGACAT

Downstream 100 bases:

>100_bases
AGAGAAGGTGAGAGAATGAGTAAATTAGTCGTTATTGGAGGCGGACCTGCTGGCTATGTAGCGGCAATTACCGCGGCTCA
AAATGGAAAAAATGTCACTC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Acetoin dehydrogenase E2 component; Dihydrolipoamide acetyltransferase component of acetoin cleaving system [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MAVEVVMPKLGMAMKEGIITSWNIKAGDHVSKGELIASINSEKIETEIEAPADGTVLDIAVSEDEGVPPGTVICYIGKPN
EKVEVHESTKVEEELVASEVSQNVQHPEPLGKEVTNKQRIKISPVAKKIAKTENLDIKSLLGTGPGGRITKVDVLKALEE
RVAIPEVLEESKVIPVTGMRKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAKVVQKRYNNKLTITDFVSRAVVLA
LGEHKEMNSAYMDDTIHQFEHVHLGMAIALEKGLVVPAIRFANNLSLVELSKQIKNVAQKARAGSLSSDDMQGTTFTISN
LGSFGIEYFTPVLNTPEAGILGVGAIEHVPVYKGKKLKKGSMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL

Sequences:

>Translated_398_residues
MAVEVVMPKLGMAMKEGIITSWNIKAGDHVSKGELIASINSEKIETEIEAPADGTVLDIAVSEDEGVPPGTVICYIGKPN
EKVEVHESTKVEEELVASEVSQNVQHPEPLGKEVTNKQRIKISPVAKKIAKTENLDIKSLLGTGPGGRITKVDVLKALEE
RVAIPEVLEESKVIPVTGMRKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAKVVQKRYNNKLTITDFVSRAVVLA
LGEHKEMNSAYMDDTIHQFEHVHLGMAIALEKGLVVPAIRFANNLSLVELSKQIKNVAQKARAGSLSSDDMQGTTFTISN
LGSFGIEYFTPVLNTPEAGILGVGAIEHVPVYKGKKLKKGSMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL
>Mature_397_residues
AVEVVMPKLGMAMKEGIITSWNIKAGDHVSKGELIASINSEKIETEIEAPADGTVLDIAVSEDEGVPPGTVICYIGKPNE
KVEVHESTKVEEELVASEVSQNVQHPEPLGKEVTNKQRIKISPVAKKIAKTENLDIKSLLGTGPGGRITKVDVLKALEER
VAIPEVLEESKVIPVTGMRKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAKVVQKRYNNKLTITDFVSRAVVLAL
GEHKEMNSAYMDDTIHQFEHVHLGMAIALEKGLVVPAIRFANNLSLVELSKQIKNVAQKARAGSLSSDDMQGTTFTISNL
GSFGIEYFTPVLNTPEAGILGVGAIEHVPVYKGKKLKKGSMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL

Specific function: The 2-Oxoglutarate Dehydrogenase Complex Catalyzes The Overall Conversion Of 2-Oxoglutarate To Succinyl-CoA And Co(2). It Contains Multiple Copies Of Three Enzymatic Components: 2- Oxoglutarate Dehydrogenase (E1), Dihydrolipoamide Succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=431, Percent_Identity=31.0904872389791, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI110671329, Length=429, Percent_Identity=26.5734265734266, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI203098753, Length=463, Percent_Identity=26.133909287257, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI203098816, Length=463, Percent_Identity=26.133909287257, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI19923748, Length=223, Percent_Identity=34.9775784753363, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI260898739, Length=158, Percent_Identity=34.1772151898734, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1786946, Length=410, Percent_Identity=31.219512195122, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1786305, Length=419, Percent_Identity=27.6849642004773, Blast_Score=152, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17560088, Length=430, Percent_Identity=30.2325581395349, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI25146366, Length=408, Percent_Identity=28.6764705882353, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17537937, Length=439, Percent_Identity=24.8291571753986, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=227, Percent_Identity=28.1938325991189, Blast_Score=98, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=401, Percent_Identity=30.4239401496259, Blast_Score=172, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6324258, Length=446, Percent_Identity=26.6816143497758, Blast_Score=167, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24582497, Length=291, Percent_Identity=31.2714776632302, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI20129315, Length=291, Percent_Identity=31.2714776632302, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI18859875, Length=431, Percent_Identity=25.754060324826, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24645909, Length=219, Percent_Identity=32.8767123287671, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 43241; Mature: 43110

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEVVMPKLGMAMKEGIITSWNIKAGDHVSKGELIASINSEKIETEIEAPADGTVLDIA
CCEEEECHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCHHHHEEECCCCCCCEEEEE
VSEDEGVPPGTVICYIGKPNEKVEVHESTKVEEELVASEVSQNVQHPEPLGKEVTNKQRI
ECCCCCCCCCEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCEE
KISPVAKKIAKTENLDIKSLLGTGPGGRITKVDVLKALEERVAIPEVLEESKVIPVTGMR
EECHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHH
KAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAKVVQKRYNNKLTITDFVSRAVVLA
HHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHH
LGEHKEMNSAYMDDTIHQFEHVHLGMAIALEKGLVVPAIRFANNLSLVELSKQIKNVAQK
CCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCEEHHHHHHCCCHHHHHHHHHHHHHHH
ARAGSLSSDDMQGTTFTISNLGSFGIEYFTPVLNTPEAGILGVGAIEHVPVYKGKKLKKG
HHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCEEECCCHHCCCCCCCCCCCCC
SMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL
CCCEEEEEECCHHCCCCCHHHHHHHHHHHHCCCCEECC
>Mature Secondary Structure 
AVEVVMPKLGMAMKEGIITSWNIKAGDHVSKGELIASINSEKIETEIEAPADGTVLDIA
CEEEECHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCHHHHEEECCCCCCCEEEEE
VSEDEGVPPGTVICYIGKPNEKVEVHESTKVEEELVASEVSQNVQHPEPLGKEVTNKQRI
ECCCCCCCCCEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCEE
KISPVAKKIAKTENLDIKSLLGTGPGGRITKVDVLKALEERVAIPEVLEESKVIPVTGMR
EECHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHH
KAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAKVVQKRYNNKLTITDFVSRAVVLA
HHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHH
LGEHKEMNSAYMDDTIHQFEHVHLGMAIALEKGLVVPAIRFANNLSLVELSKQIKNVAQK
CCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCEEHHHHHHCCCHHHHHHHHHHHHHHH
ARAGSLSSDDMQGTTFTISNLGSFGIEYFTPVLNTPEAGILGVGAIEHVPVYKGKKLKKG
HHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCEEECCCHHCCCCCCCCCCCCC
SMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL
CCCEEEEEECCHHCCCCCHHHHHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]