Click here to switch to the map view.

The map label for this gene is oppA [H]

Identifier: 52142665

GI number: 52142665

Start: 2696754

End: 2698376

Strand: Reverse

Name: oppA [H]

Synonym: BCZK2576

Alternate gene names: 52142665

Gene position: 2698376-2696754 (Counterclockwise)

Preceding gene: 52142663

Following gene: 52142664

Centisome position: 50.9

GC content: 34.63

Gene sequence:

>1623_bases
ATGAAAAAATTTGTTCGCTACTCATTAGTTAGTACTCTATTCGTTTCTTCGATTTTAGTTGGCTGCGCAAAAGAAAAAAC
AACGACAAAGCCGAAAGATGAGAAGAAAGTATTACAGTTACTAGAAACGGGTGAAATTCCATCGTTAAATTCAGGGAAAG
TAACAGATGCAGTATCATTCAACGTTTTAAATAACATAATGGAAGGATTATTCCGTTTATCGAAAGATGATGAAGTGATT
GAGGCTGGAGCACAAAAATATGAAGTGAGTAAAGATGGGAAAACATATACATTCCACTTGCGTGATGCGAAATGGTCTAA
CGGAGATCCAGTAACAGCACAAGATTACGTATATGCTTGGAAGCAGCTTATTAACCCAGATACAGCTTCTCAATATGCAT
ACATTGCGTACGATGTGAAAAATGCAGAGAAAATAAATAAGAAACAACTAGGGGTAGAGGAATTAGGAGTAAAAGCAAAG
GATGATAAAACGTTCGTTGTAGAGCTAGAACATCCTGTACCGTATTTTACGAAACTACTTATTTTACCGTCCTTCTATCC
AATTAACGAGAAATATGCGAAAGAGCAAGGCGATAAATATGGATTAGAAGCAAATAAAGCGGTATATAATGGGCCATTTA
CATTATCGGAGTGGAAGCATGAAGCAAGTTTTACAATGAAGAAAAACGATAAGTATTGGGATAAAAAAGAAGTGAAGCTA
GATGAAGTAAACTATCAAATTGTTAAAGAAATTTCAACTGCGGTAAATTTATATGAAACAGATAAGGTTGATAGAGCTGT
CATTTCTACAGAATTCGTCGATAAATATAAAAATAATAAAGAGCTGAAACAATATACAGATCCGGTTATGTACTTTTTCC
GATTTAATGAAAATGTACCGATTCTTAAAAATAAAAATGCACGTCTTGCGCTTAGTACAGTTTTTGATAAGAAAGGACTT
GCGACTTCGTTTTTAAATGATGGTTCGGTTGCGGCGAACTATTACGTACCAAAAGGATTTTTAAAAGGTCCAGATAAAAA
AGATTTTAGAAGTACGGCAGGTGAGTTTAATAAGACGGATGTAAAACAGGCGAAAGAATATTGGGAAAAGGCGAAGCAAG
AAACAGGTATAAATGAAGTGACGCTTGAGTTATTAAACTATGACTTAGAAAACTTCAAAAAAGTAGGAGAATATATTAAA
GAGCAGCTTGAGAAAAACTTACCGGGCTTAAAGGTAAATGTGAAATTACAACCACACACGCAAAAATTAGCGCTAGAGAA
GAAGAAAGAATATGAAATGTCGTTATCACGTTGGTTACCAGATTATCCAGATCCAATGACATACTTAGAAGTATTCCTTT
CTGGAAGTAGTGTGAACAATACAGAATATGCAAATCCGGAATATGATGCGTTAATTAAGAAGATTAAAACAGAATTAGGT
AATGATGAAAAGGCTCGCTGGAAAGCACTACAAGATGCTGAAAAAATGCTACTTGATGATGCAGTTATTGCTCCAGTATT
TCAGCGCGGATTATCTTACTTACAAAAGCCATATGTGAAAGATTTATACGTACATCAATTTGGACCAGCAACAAGTTTAA
AATGGGCAGATGTGCAAAAATAA

Upstream 100 bases:

>100_bases
AAAGATCAGTAGAGATTATACCGTTAAATACACCTGGATATATAGAAGAATACGCTGGTGCTCGTCGTTACTTACCTTAA
AAAATAGAGGGGGTTTTCAA

Downstream 100 bases:

>100_bases
ACGATGAGTAAACAGACTCCACGTTTGTGGGAGTCTGTTTTTTTAGTTAAAGTCAGGTAAATAGTACATGGTATAATTTA
CTAGTCTGTATAAAATTTTG

Product: oligopeptide ABC transporter, substrate-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MKKFVRYSLVSTLFVSSILVGCAKEKTTTKPKDEKKVLQLLETGEIPSLNSGKVTDAVSFNVLNNIMEGLFRLSKDDEVI
EAGAQKYEVSKDGKTYTFHLRDAKWSNGDPVTAQDYVYAWKQLINPDTASQYAYIAYDVKNAEKINKKQLGVEELGVKAK
DDKTFVVELEHPVPYFTKLLILPSFYPINEKYAKEQGDKYGLEANKAVYNGPFTLSEWKHEASFTMKKNDKYWDKKEVKL
DEVNYQIVKEISTAVNLYETDKVDRAVISTEFVDKYKNNKELKQYTDPVMYFFRFNENVPILKNKNARLALSTVFDKKGL
ATSFLNDGSVAANYYVPKGFLKGPDKKDFRSTAGEFNKTDVKQAKEYWEKAKQETGINEVTLELLNYDLENFKKVGEYIK
EQLEKNLPGLKVNVKLQPHTQKLALEKKKEYEMSLSRWLPDYPDPMTYLEVFLSGSSVNNTEYANPEYDALIKKIKTELG
NDEKARWKALQDAEKMLLDDAVIAPVFQRGLSYLQKPYVKDLYVHQFGPATSLKWADVQK

Sequences:

>Translated_540_residues
MKKFVRYSLVSTLFVSSILVGCAKEKTTTKPKDEKKVLQLLETGEIPSLNSGKVTDAVSFNVLNNIMEGLFRLSKDDEVI
EAGAQKYEVSKDGKTYTFHLRDAKWSNGDPVTAQDYVYAWKQLINPDTASQYAYIAYDVKNAEKINKKQLGVEELGVKAK
DDKTFVVELEHPVPYFTKLLILPSFYPINEKYAKEQGDKYGLEANKAVYNGPFTLSEWKHEASFTMKKNDKYWDKKEVKL
DEVNYQIVKEISTAVNLYETDKVDRAVISTEFVDKYKNNKELKQYTDPVMYFFRFNENVPILKNKNARLALSTVFDKKGL
ATSFLNDGSVAANYYVPKGFLKGPDKKDFRSTAGEFNKTDVKQAKEYWEKAKQETGINEVTLELLNYDLENFKKVGEYIK
EQLEKNLPGLKVNVKLQPHTQKLALEKKKEYEMSLSRWLPDYPDPMTYLEVFLSGSSVNNTEYANPEYDALIKKIKTELG
NDEKARWKALQDAEKMLLDDAVIAPVFQRGLSYLQKPYVKDLYVHQFGPATSLKWADVQK
>Mature_540_residues
MKKFVRYSLVSTLFVSSILVGCAKEKTTTKPKDEKKVLQLLETGEIPSLNSGKVTDAVSFNVLNNIMEGLFRLSKDDEVI
EAGAQKYEVSKDGKTYTFHLRDAKWSNGDPVTAQDYVYAWKQLINPDTASQYAYIAYDVKNAEKINKKQLGVEELGVKAK
DDKTFVVELEHPVPYFTKLLILPSFYPINEKYAKEQGDKYGLEANKAVYNGPFTLSEWKHEASFTMKKNDKYWDKKEVKL
DEVNYQIVKEISTAVNLYETDKVDRAVISTEFVDKYKNNKELKQYTDPVMYFFRFNENVPILKNKNARLALSTVFDKKGL
ATSFLNDGSVAANYYVPKGFLKGPDKKDFRSTAGEFNKTDVKQAKEYWEKAKQETGINEVTLELLNYDLENFKKVGEYIK
EQLEKNLPGLKVNVKLQPHTQKLALEKKKEYEMSLSRWLPDYPDPMTYLEVFLSGSSVNNTEYANPEYDALIKKIKTELG
NDEKARWKALQDAEKMLLDDAVIAPVFQRGLSYLQKPYVKDLYVHQFGPATSLKWADVQK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=534, Percent_Identity=29.2134831460674, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI87081878, Length=535, Percent_Identity=29.7196261682243, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1789397, Length=489, Percent_Identity=29.038854805726, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1787762, Length=439, Percent_Identity=22.7790432801822, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789966, Length=479, Percent_Identity=22.5469728601253, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62067; Mature: 62067

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFVRYSLVSTLFVSSILVGCAKEKTTTKPKDEKKVLQLLETGEIPSLNSGKVTDAVSF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHH
NVLNNIMEGLFRLSKDDEVIEAGAQKYEVSKDGKTYTFHLRDAKWSNGDPVTAQDYVYAW
HHHHHHHHHHHHCCCCHHHHHCCCHHEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHH
KQLINPDTASQYAYIAYDVKNAEKINKKQLGVEELGVKAKDDKTFVVELEHPVPYFTKLL
HHHCCCCCCCCEEEEEEECCCHHHHHHHHCCHHHHCCCCCCCCEEEEEECCCCHHHHHHH
ILPSFYPINEKYAKEQGDKYGLEANKAVYNGPFTLSEWKHEASFTMKKNDKYWDKKEVKL
HHCCCCCCCHHHHHHCCCCCCCCCCCEEECCCCCHHHHCCCCCEEECCCCCCCCCCCCCH
DEVNYQIVKEISTAVNLYETDKVDRAVISTEFVDKYKNNKELKQYTDPVMYFFRFNENVP
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEEECCCCC
ILKNKNARLALSTVFDKKGLATSFLNDGSVAANYYVPKGFLKGPDKKDFRSTAGEFNKTD
EEECCCCEEEEEEHHCCCCCHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCHHH
VKQAKEYWEKAKQETGINEVTLELLNYDLENFKKVGEYIKEQLEKNLPGLKVNVKLQPHT
HHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCH
QKLALEKKKEYEMSLSRWLPDYPDPMTYLEVFLSGSSVNNTEYANPEYDALIKKIKTELG
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHC
NDEKARWKALQDAEKMLLDDAVIAPVFQRGLSYLQKPYVKDLYVHQFGPATSLKWADVQK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKFVRYSLVSTLFVSSILVGCAKEKTTTKPKDEKKVLQLLETGEIPSLNSGKVTDAVSF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHH
NVLNNIMEGLFRLSKDDEVIEAGAQKYEVSKDGKTYTFHLRDAKWSNGDPVTAQDYVYAW
HHHHHHHHHHHHCCCCHHHHHCCCHHEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHH
KQLINPDTASQYAYIAYDVKNAEKINKKQLGVEELGVKAKDDKTFVVELEHPVPYFTKLL
HHHCCCCCCCCEEEEEEECCCHHHHHHHHCCHHHHCCCCCCCCEEEEEECCCCHHHHHHH
ILPSFYPINEKYAKEQGDKYGLEANKAVYNGPFTLSEWKHEASFTMKKNDKYWDKKEVKL
HHCCCCCCCHHHHHHCCCCCCCCCCCEEECCCCCHHHHCCCCCEEECCCCCCCCCCCCCH
DEVNYQIVKEISTAVNLYETDKVDRAVISTEFVDKYKNNKELKQYTDPVMYFFRFNENVP
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEEECCCCC
ILKNKNARLALSTVFDKKGLATSFLNDGSVAANYYVPKGFLKGPDKKDFRSTAGEFNKTD
EEECCCCEEEEEEHHCCCCCHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCHHH
VKQAKEYWEKAKQETGINEVTLELLNYDLENFKKVGEYIKEQLEKNLPGLKVNVKLQPHT
HHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCH
QKLALEKKKEYEMSLSRWLPDYPDPMTYLEVFLSGSSVNNTEYANPEYDALIKKIKTELG
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHC
NDEKARWKALQDAEKMLLDDAVIAPVFQRGLSYLQKPYVKDLYVHQFGPATSLKWADVQK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]