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The map label for this gene is phoB [H]

Identifier: 52142651

GI number: 52142651

Start: 2709080

End: 2709727

Strand: Reverse

Name: phoB [H]

Synonym: BCZK2590

Alternate gene names: 52142651

Gene position: 2709727-2709080 (Counterclockwise)

Preceding gene: 52142650

Following gene: 52142652

Centisome position: 51.12

GC content: 31.94

Gene sequence:

>648_bases
ATGAGAGTATTAATCGCAGATGATGAACAAGATATGCTAAGGATTTTAAAAGCTTATTTTGAAAAAGCAGATTTCGAAGT
GTTATTAGCGAAAGATGGAGAAGAAGCACTTCAAATATTTTATGATGAAAAAATTGATTTAGCTATTTTAGATTGGATGA
TGCCGAAACATAGCGGTATTACTGTTTGTAAGGAAATAAAGAAAAACTCAAGCGTAAAAGTATTAATGCTTACGGCGAAA
AGTGAAAGCGAAGATGAATTAGCAGCTCTTCAAAGCGGGGCAGATGAGTACGTAAAAAAACCATTTCATCCAGGTGTACT
CATTACTCGGGCGAAAAAGCTAATACAGCACGATGATGTCATTCAAGTTCAAGATTTGAAAATTGATTTTACGAAAAATA
AAGTTTATAAAAATGACAAAGAATTAGATATTACAAAAACAGAGTTAGAATTAATAAAATGTTTTTTAAATCATAAAGGG
ATGATTTTAACTCGTAAAAAATTGTTAGATATTGTATGGGGATTTGACTATTTTGGAGAAGAGCGAACTGTTGATACACA
TGTAAGGAGATTACGAAAAAAAGTTGGAGAAAATATTATAAAGACGCACCGCGGGCTAGGATATAGTTTGGAGGAACAGA
GTGAATAA

Upstream 100 bases:

>100_bases
TATACCCTTAACTGGTTCAAATATTTTTCTGTCACATACATGTAACAAAGAAGTTGTATGATACAATTGATTTTTGTTTA
AAAGCAATGGAGGATAATGT

Downstream 100 bases:

>100_bases
ACTTGGAAAAAAGCTATTTCTTAGTATATCTTTAACCGTTATTCTTATATTTACGATCTCTTTATTATTAATTAACTATC
TTTTACCGAAATATAATATT

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MRVLIADDEQDMLRILKAYFEKADFEVLLAKDGEEALQIFYDEKIDLAILDWMMPKHSGITVCKEIKKNSSVKVLMLTAK
SESEDELAALQSGADEYVKKPFHPGVLITRAKKLIQHDDVIQVQDLKIDFTKNKVYKNDKELDITKTELELIKCFLNHKG
MILTRKKLLDIVWGFDYFGEERTVDTHVRRLRKKVGENIIKTHRGLGYSLEEQSE

Sequences:

>Translated_215_residues
MRVLIADDEQDMLRILKAYFEKADFEVLLAKDGEEALQIFYDEKIDLAILDWMMPKHSGITVCKEIKKNSSVKVLMLTAK
SESEDELAALQSGADEYVKKPFHPGVLITRAKKLIQHDDVIQVQDLKIDFTKNKVYKNDKELDITKTELELIKCFLNHKG
MILTRKKLLDIVWGFDYFGEERTVDTHVRRLRKKVGENIIKTHRGLGYSLEEQSE
>Mature_215_residues
MRVLIADDEQDMLRILKAYFEKADFEVLLAKDGEEALQIFYDEKIDLAILDWMMPKHSGITVCKEIKKNSSVKVLMLTAK
SESEDELAALQSGADEYVKKPFHPGVLITRAKKLIQHDDVIQVQDLKIDFTKNKVYKNDKELDITKTELELIKCFLNHKG
MILTRKKLLDIVWGFDYFGEERTVDTHVRRLRKKVGENIIKTHRGLGYSLEEQSE

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=220, Percent_Identity=35, Blast_Score=150, Evalue=5e-38,
Organism=Escherichia coli, GI1789809, Length=225, Percent_Identity=28, Blast_Score=123, Evalue=8e-30,
Organism=Escherichia coli, GI1788394, Length=222, Percent_Identity=31.0810810810811, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1786911, Length=218, Percent_Identity=30.2752293577982, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=33.0316742081448, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1786784, Length=222, Percent_Identity=31.0810810810811, Blast_Score=113, Evalue=8e-27,
Organism=Escherichia coli, GI87082012, Length=218, Percent_Identity=27.9816513761468, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1787229, Length=221, Percent_Identity=25.7918552036199, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI1787375, Length=221, Percent_Identity=28.0542986425339, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI2367329, Length=225, Percent_Identity=29.7777777777778, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790863, Length=227, Percent_Identity=29.9559471365639, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI145693140, Length=228, Percent_Identity=27.1929824561404, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1790552, Length=224, Percent_Identity=29.9107142857143, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789402, Length=219, Percent_Identity=26.4840182648402, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI1788550, Length=102, Percent_Identity=38.2352941176471, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790437, Length=101, Percent_Identity=35.6435643564356, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 24958; Mature: 24958

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLIADDEQDMLRILKAYFEKADFEVLLAKDGEEALQIFYDEKIDLAILDWMMPKHSGI
CEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCEEHHHHHCCCCCCH
TVCKEIKKNSSVKVLMLTAKSESEDELAALQSGADEYVKKPFHPGVLITRAKKLIQHDDV
HHHHHHHCCCCEEEEEEEECCCCHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHCCCCC
IQVQDLKIDFTKNKVYKNDKELDITKTELELIKCFLNHKGMILTRKKLLDIVWGFDYFGE
EEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCHHHCCC
ERTVDTHVRRLRKKVGENIIKTHRGLGYSLEEQSE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRVLIADDEQDMLRILKAYFEKADFEVLLAKDGEEALQIFYDEKIDLAILDWMMPKHSGI
CEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCEEHHHHHCCCCCCH
TVCKEIKKNSSVKVLMLTAKSESEDELAALQSGADEYVKKPFHPGVLITRAKKLIQHDDV
HHHHHHHCCCCEEEEEEEECCCCHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHCCCCC
IQVQDLKIDFTKNKVYKNDKELDITKTELELIKCFLNHKGMILTRKKLLDIVWGFDYFGE
EEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCHHHCCC
ERTVDTHVRRLRKKVGENIIKTHRGLGYSLEEQSE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2115874; 10984043 [H]