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The map label for this gene is emrB [H]

Identifier: 52142634

GI number: 52142634

Start: 2724983

End: 2726518

Strand: Reverse

Name: emrB [H]

Synonym: BCZK2608

Alternate gene names: 52142634

Gene position: 2726518-2724983 (Counterclockwise)

Preceding gene: 52142632

Following gene: 52142636

Centisome position: 51.43

GC content: 37.3

Gene sequence:

>1536_bases
ATGGTTGAGAAGAATAATAAGCTCGGCTTTGTTGTGGCGGGCTTATTGCTAGGTATTTTAATGGCATCAATGGATAATAC
CATTGTCGTAACAGCGATGGGAACGATTGTTGGTGACTTAGGAGGTCTTGAAAACTTTGTATGGGTCGTTTCTGCCTATA
TGGTCGCAGAAATGGCAGGTATGCCGATTTTCGGTAAACTATCAGATATGTATGGTAGAAAGAGATTCTTTATTTTTGGT
TTAATCGTCTTTATGGTTGGTTCGGCACTTTGTGGTACTGCTGAAAATATTACACAGTTAGGTATTTATCGTGCCATTCA
AGGTATTGGCGGCGGGGCATTAGTGCCGATCGCGTTTACAATCGTTTTTGATATTTTCCCTCCTGAAAAGCGCGGGAAAA
TGGGTGGTTTATTTGGAGCGGTATTTGGTTTATCAAGTATTTTCGGGCCGTTACTGGGCGCATATATTACAGATTATATT
AGCTGGCACTGGGTATTTTACATTAACTTACCTCTTGGCGTTTTAGCACTTATTTTTATTACATTCTTTTATAAAGAATC
ACGAGTTCATAGAAAGCAAAAAATTGATTGGTTTGGCGCAATTACTTTAGTTGGTGCAGTAGTTTCGTTAATGTTTGCTT
TAGAACTTGGCGGACAAAAGTATGACTGGGATTCTAGCTTTATTTTAAGTTTATTTGGTGGATTCGTTATTTTAATTATT
GCATTTATATTTATTGAACGAAAAGTAGAAGAGCCAATTATATCATTTGAGATGTTTAAGCAGCGCTTATTCGGAATGAG
TACCATTATTGCTTTATGTTACGGTGCTGCGTTTATGTCGGCAACTGTATATATTCCGTTATTCATTCAAGGTGTATACG
GAGGTAGTGCAACAAACTCAGGGTTATTACTTTTACCGATGATGTTAGGATCAGTTGTAACAGCGCAGTTAGGCGGATTT
TTAACGACGAAGCTTAGCTACCGAAACATTATGATTATTTCTGCGGTTATTATGCTAATTGGACTATTTTTATTAAGCAC
ATTAACCCCAGAAACAAGTCGCGCATTATTAACAGTGTATATGATTATTATCGGATTTGGAGTTGGATTCTCATTCTCTG
TACTAAGTATGGCAGCTATTCACAACTTTGGTATGGAGCAGCGTGGATCTGCGACCTCAACGAGTAATTTCATTCGTTCA
TTAGGAATGACGCTTGGTATTACAATCTTCGGAATGATTCAAAGAACAGGTTTCCAAGATCAATTAGAAGAAGCATTTAA
AGGGATGAGCGGGGGAATGAATACAAACACTTTAGGAGATTCAAGAGCTATTCTATCAGAATCGGCAAGATCTCAAATCC
CGCCGCAAATATTAGATAAAATTATTGACGCTCTTTCTAGCTCAATTGTCCAAACATTTATGTGGGCATTGGTACCAGCT
GGTTTAGCATTCTTATTTATTTTCTTTATGGGAAATGAGCGCATGGTAATTAAGAAACAACAAAAGAATAAAAAGAACGA
AACATCAAAAGCGTAA

Upstream 100 bases:

>100_bases
AGATTAGTGTTGTAGAATATCATGCTAGTTTTAATCAAAAGGGTAATACAGTCGTAAAAGAACATAAACAAATATAAGTA
GTATGAGGGAGAGCAAGGTT

Downstream 100 bases:

>100_bases
TGAAAAAGCTCCTTTCCAATTGGAAAGGAGCTTTTTCGTATGTACGGTTTTAGATTAGCCTTCGATGATTTCGTATTCTT
CGTCTGCTAATAATAAAATT

Product: multidrug resistance protein B

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MVEKNNKLGFVVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAGMPIFGKLSDMYGRKRFFIFG
LIVFMVGSALCGTAENITQLGIYRAIQGIGGGALVPIAFTIVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYI
SWHWVFYINLPLGVLALIFITFFYKESRVHRKQKIDWFGAITLVGAVVSLMFALELGGQKYDWDSSFILSLFGGFVILII
AFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGSATNSGLLLLPMMLGSVVTAQLGGF
LTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVYMIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRS
LGMTLGITIFGMIQRTGFQDQLEEAFKGMSGGMNTNTLGDSRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPA
GLAFLFIFFMGNERMVIKKQQKNKKNETSKA

Sequences:

>Translated_511_residues
MVEKNNKLGFVVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAGMPIFGKLSDMYGRKRFFIFG
LIVFMVGSALCGTAENITQLGIYRAIQGIGGGALVPIAFTIVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYI
SWHWVFYINLPLGVLALIFITFFYKESRVHRKQKIDWFGAITLVGAVVSLMFALELGGQKYDWDSSFILSLFGGFVILII
AFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGSATNSGLLLLPMMLGSVVTAQLGGF
LTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVYMIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRS
LGMTLGITIFGMIQRTGFQDQLEEAFKGMSGGMNTNTLGDSRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPA
GLAFLFIFFMGNERMVIKKQQKNKKNETSKA
>Mature_511_residues
MVEKNNKLGFVVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAGMPIFGKLSDMYGRKRFFIFG
LIVFMVGSALCGTAENITQLGIYRAIQGIGGGALVPIAFTIVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYI
SWHWVFYINLPLGVLALIFITFFYKESRVHRKQKIDWFGAITLVGAVVSLMFALELGGQKYDWDSSFILSLFGGFVILII
AFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGSATNSGLLLLPMMLGSVVTAQLGGF
LTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVYMIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRS
LGMTLGITIFGMIQRTGFQDQLEEAFKGMSGGMNTNTLGDSRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPA
GLAFLFIFFMGNERMVIKKQQKNKKNETSKA

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=403, Percent_Identity=30.272952853598, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI87081983, Length=404, Percent_Identity=29.2079207920792, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1788710, Length=441, Percent_Identity=28.3446712018141, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1790195, Length=421, Percent_Identity=24.9406175771971, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788392, Length=405, Percent_Identity=25.9259259259259, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790146, Length=162, Percent_Identity=29.0123456790123, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI48994889, Length=134, Percent_Identity=31.3432835820896, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=554, Percent_Identity=24.187725631769, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6319770, Length=399, Percent_Identity=27.8195488721804, Blast_Score=130, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6319783, Length=457, Percent_Identity=24.507658643326, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6323735, Length=324, Percent_Identity=30.5555555555556, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6325455, Length=529, Percent_Identity=23.062381852552, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321663, Length=540, Percent_Identity=22.2222222222222, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324954, Length=398, Percent_Identity=24.6231155778894, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323520, Length=410, Percent_Identity=24.390243902439, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55784; Mature: 55784

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCEEEEEEHHCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHCCCEEEEEEHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]