BacMap

Definition	Bacillus cereus E33L, complete genome.
Accession	NC_006274
Length	5,300,915

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The map label for this gene is fadB [H]

Identifier: 52142554

GI number: 52142554

Start: 2803674

End: 2804465

Strand: Reverse

Name: fadB [H]

Synonym: BCZK2687

Alternate gene names: 52142554

Gene position: 2804465-2803674 (Counterclockwise)

Preceding gene: 52142555

Following gene: 52142556

Centisome position: 52.91

GC content: 41.79

Gene sequence:

>792_bases
TTGGAAAGTAATAGAGTTGTTACATGGTCCAAAGAAAATGGAATTGCGACGATTATCATTGATAATCCTCCTATGAATGT
ATTGGGTAGTGCCGTGGTGGAGCAACTGACGGTAGCGGTCGATACAATCGAACGGGATGATGAAGTAATTGTTGTACTTC
TAACGGGTGCTGGAGAAAAGACCTTTGTTGCAGGAGGGGACATTAAGTCATTTCCGGAATGGATTGGGAAAGGGAGTGAA
TATGCAGAGGAAAAATCATTGTGGCTTCAGAATCCATTAAATAAAATAGAAAGATTGCCAAAGCCAGTAATCGCTGCCAT
TAACGGATTAGCGCTAGGCGGTGGGTGTGAATTAGCTTTATCGTGTGACATACGAATTATTGAAGAACACGCCCAAATCG
GGCTTCCGGAAGTGAAATTAGGTTTATTTCCTGGTGCAGGAGGAACGCAACGTTTGCCGAGATTAATAGGAACTGCAAGT
GCGAAGGAAATGATGTTTACTGGTGAACCACTCACTGCCGAAGCAGCTTGGCGAGTTGGTTTAGTGAATCATATTGTTCC
GCGTGGTGAATCACTGAATAAGGCGAAGGAATTAGCCGCGAAAATGGCTCGATTTTCTCTTCCTGCTTTATCTTTGATGA
AACAATCTATCGATAAAGGGTTATCTTCTTCTCTTGAAGAAGGATTAAAAATAGAAGCTGAGAATTTCGGACATGTATTT
CAAACGAGTGATGTAAGAGAGGGAGTGGAGGCCTTTATAGAAAAGAGAGCACCATTCTTTCATCATAAATAA

Upstream 100 bases:

>100_bases
TCGGCAGCGCTTGGGATCGCGAAAAGGTCGGTTATGTAATTAAAAGAATGAGTTAATGTATTGATTTTACCTTATTACAT
TTTGGAAAGAGGGGATTGGA

Downstream 100 bases:

>100_bases
AAGGCTATAACTTATAATATGCAAAAAGTAATGCCTGCCAGAAGCAGGCATTACAACGATATATACAAAGTAAAAAAATC
CATATTATTGAGGGGGATTG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MESNRVVTWSKENGIATIIIDNPPMNVLGSAVVEQLTVAVDTIERDDEVIVVLLTGAGEKTFVAGGDIKSFPEWIGKGSE
YAEEKSLWLQNPLNKIERLPKPVIAAINGLALGGGCELALSCDIRIIEEHAQIGLPEVKLGLFPGAGGTQRLPRLIGTAS
AKEMMFTGEPLTAEAAWRVGLVNHIVPRGESLNKAKELAAKMARFSLPALSLMKQSIDKGLSSSLEEGLKIEAENFGHVF
QTSDVREGVEAFIEKRAPFFHHK

Sequences:

>Translated_263_residues
MESNRVVTWSKENGIATIIIDNPPMNVLGSAVVEQLTVAVDTIERDDEVIVVLLTGAGEKTFVAGGDIKSFPEWIGKGSE
YAEEKSLWLQNPLNKIERLPKPVIAAINGLALGGGCELALSCDIRIIEEHAQIGLPEVKLGLFPGAGGTQRLPRLIGTAS
AKEMMFTGEPLTAEAAWRVGLVNHIVPRGESLNKAKELAAKMARFSLPALSLMKQSIDKGLSSSLEEGLKIEAENFGHVF
QTSDVREGVEAFIEKRAPFFHHK
>Mature_263_residues
MESNRVVTWSKENGIATIIIDNPPMNVLGSAVVEQLTVAVDTIERDDEVIVVLLTGAGEKTFVAGGDIKSFPEWIGKGSE
YAEEKSLWLQNPLNKIERLPKPVIAAINGLALGGGCELALSCDIRIIEEHAQIGLPEVKLGLFPGAGGTQRLPRLIGTAS
AKEMMFTGEPLTAEAAWRVGLVNHIVPRGESLNKAKELAAKMARFSLPALSLMKQSIDKGLSSSLEEGLKIEAENFGHVF
QTSDVREGVEAFIEKRAPFFHHK

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=254, Percent_Identity=37.7952755905512, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI4502327, Length=239, Percent_Identity=38.9121338912134, Blast_Score=127, Evalue=7e-30,
Organism=Homo sapiens, GI31542718, Length=263, Percent_Identity=30.7984790874525, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI68989263, Length=248, Percent_Identity=33.4677419354839, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI20127408, Length=257, Percent_Identity=31.5175097276265, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI150378541, Length=257, Percent_Identity=33.0739299610895, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=31.203007518797, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI157694516, Length=234, Percent_Identity=28.2051282051282, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI213417737, Length=234, Percent_Identity=28.2051282051282, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI37594469, Length=185, Percent_Identity=30.8108108108108, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI37594471, Length=185, Percent_Identity=30.8108108108108, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI261878539, Length=148, Percent_Identity=35.8108108108108, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI62530384, Length=249, Percent_Identity=26.1044176706827, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI4757968, Length=259, Percent_Identity=24.3243243243243, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI49355787, Length=259, Percent_Identity=24.3243243243243, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI25453481, Length=259, Percent_Identity=24.3243243243243, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI51538573, Length=259, Percent_Identity=24.3243243243243, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI157694520, Length=191, Percent_Identity=27.7486910994764, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI4757966, Length=253, Percent_Identity=24.5059288537549, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI51538581, Length=253, Percent_Identity=24.5059288537549, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI221307494, Length=203, Percent_Identity=29.5566502463054, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI221136756, Length=203, Percent_Identity=29.5566502463054, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI221136753, Length=203, Percent_Identity=29.5566502463054, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI45643119, Length=215, Percent_Identity=28.8372093023256, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI260275230, Length=215, Percent_Identity=28.8372093023256, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI260274832, Length=215, Percent_Identity=28.8372093023256, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI295842266, Length=247, Percent_Identity=24.6963562753036, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI116235487, Length=193, Percent_Identity=29.0155440414508, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=36.8627450980392, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI221142681, Length=258, Percent_Identity=32.5581395348837, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1788682, Length=213, Percent_Identity=40.3755868544601, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1790281, Length=185, Percent_Identity=38.9189189189189, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788597, Length=253, Percent_Identity=31.6205533596838, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787660, Length=254, Percent_Identity=33.8582677165354, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87082183, Length=247, Percent_Identity=27.5303643724696, Blast_Score=99, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=38.0952380952381, Blast_Score=156, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=37.007874015748, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17540714, Length=230, Percent_Identity=36.5217391304348, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17558304, Length=199, Percent_Identity=34.6733668341709, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=193, Percent_Identity=36.2694300518135, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17508953, Length=193, Percent_Identity=36.2694300518135, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17508951, Length=193, Percent_Identity=36.2694300518135, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17540306, Length=258, Percent_Identity=28.2945736434109, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17560910, Length=259, Percent_Identity=29.7297297297297, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=249, Percent_Identity=24.8995983935743, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI25144160, Length=194, Percent_Identity=30.4123711340206, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=194, Percent_Identity=30.4123711340206, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=247, Percent_Identity=25.1012145748988, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=23.4375, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=216, Percent_Identity=25.462962962963, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=185, Percent_Identity=28.6486486486486, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI20129971, Length=250, Percent_Identity=37.6, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24653477, Length=250, Percent_Identity=37.6, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=39.7637795275591, Blast_Score=165, Evalue=2e-41,
Organism=Drosophila melanogaster, GI21357171, Length=262, Percent_Identity=32.4427480916031, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=28.3524904214559, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24650670, Length=252, Percent_Identity=30.5555555555556, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI19921000, Length=193, Percent_Identity=38.860103626943, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24583077, Length=193, Percent_Identity=38.860103626943, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24583079, Length=193, Percent_Identity=38.860103626943, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI19920382, Length=200, Percent_Identity=30.5, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583165, Length=246, Percent_Identity=26.8292682926829, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24654903, Length=245, Percent_Identity=24.8979591836735, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI19921018, Length=216, Percent_Identity=28.7037037037037, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571730, Length=253, Percent_Identity=24.901185770751, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28571729, Length=253, Percent_Identity=24.901185770751, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 28464; Mature: 28464

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESNRVVTWSKENGIATIIIDNPPMNVLGSAVVEQLTVAVDTIERDDEVIVVLLTGAGEK
CCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCE
TFVAGGDIKSFPEWIGKGSEYAEEKSLWLQNPLNKIERLPKPVIAAINGLALGGGCELAL
EEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEECCCCEEEE
SCDIRIIEEHAQIGLPEVKLGLFPGAGGTQRLPRLIGTASAKEMMFTGEPLTAEAAWRVG
ECCEEEEEHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHEEECCCCCCHHHHHHHH
LVNHIVPRGESLNKAKELAAKMARFSLPALSLMKQSIDKGLSSSLEEGLKIEAENFGHVF
HHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEE
QTSDVREGVEAFIEKRAPFFHHK
ECHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MESNRVVTWSKENGIATIIIDNPPMNVLGSAVVEQLTVAVDTIERDDEVIVVLLTGAGEK
CCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCE
TFVAGGDIKSFPEWIGKGSEYAEEKSLWLQNPLNKIERLPKPVIAAINGLALGGGCELAL
EEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEECCCCEEEE
SCDIRIIEEHAQIGLPEVKLGLFPGAGGTQRLPRLIGTASAKEMMFTGEPLTAEAAWRVG
ECCEEEEEHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHEEECCCCCCHHHHHHHH
LVNHIVPRGESLNKAKELAAKMARFSLPALSLMKQSIDKGLSSSLEEGLKIEAENFGHVF
HHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEE
QTSDVREGVEAFIEKRAPFFHHK
ECHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]