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The map label for this gene is phoB [H]

Identifier: 52142523

GI number: 52142523

Start: 2838715

End: 2840388

Strand: Reverse

Name: phoB [H]

Synonym: BCZK2719

Alternate gene names: 52142523

Gene position: 2840388-2838715 (Counterclockwise)

Preceding gene: 52142520

Following gene: 52142524

Centisome position: 53.58

GC content: 37.51

Gene sequence:

>1674_bases
ATGTTTGTATTGGGTTTAAAGATGAAGAAGTTACTGTTAGTAGGAACAATGACACTAAGTATGTTCTGTACTGTAAGTAT
GACGAATAATCATACGGTGGAAGCTAAAGTGAAAAAAGTAGAACGTCAACCGAAAAATGTAATCATAATGGTTATGGACG
GAACGAGTTCTTCAGCAACTACATTAGCTCGCTTGTATAAAGGGAAACCACTTGCATTAGATGAAATTGTAACAGGGGGA
GTTCGTACATATTCGGCGGAATCAGCTATAACAGACTCAGCTCCAGCAGCCACAGCTTTAGCAACTGGGAATAAATCGAA
TTCAGGATATGTAGGGGTATTACCTACTATTGTGAGTTCACCTGGCTTAAAACCAATGAAAGAAGAGGATAAGTTACGAC
CAGTTGCCAATGTTTTAGAAGGTGCCAAACGTACGGGTCGCGCAACTGGAATTGTTGCTACAGCTGAAATTCAGCATGCT
ACTCCTGCTGGATTCTCCGCTCATCATGTGAATCGTAACAATTTCGAAGTGCTCGCGGAGCAACAAGTATATCAAAATAT
AGATGTCGTATTAGGTGGGGGAAAAGCAGCACTTCTACCTGAGACAGATAGTGGGATTCGAAAAGACGGTGGAGATTTAG
TAAAAGTGATCAAAAACAAAGGGTACGACTTTGTTGAAACGAAAGAAGCATTATTAGGCTCTAAATCTAATAAAATTTGG
GGCTCTTTCTCGCTTAATGCTTTAGCATTTGATATGGATCGTGAAGCAACAAACCCAGAGCAACCAACACTTTCTCAAAT
GACGGAAAAAGCAATTCAAACGTTATCAAAAGATAAAGATGGCTTCTTTTTATTTGTGGAAGGAAGTAAGCCAGACTGGG
CAGCTCATGTGAATGATCCAATTGGGATGATTAGTGATGTATTAGCATTTGATAATGCAGTTGCAGAAGCATTGAAGTTT
GCAAAAAAAGATGGTAATACGATGTTAATAGCCGTAGCGGACCATGGTAATAGCGGTATTTCTATTGGGAATATGAATAC
GACAAAAGGATATAATACTACGCCGGTCTCTGCGTACATTTATCCATTGAAAAAAGCGAAGATGACACTTGAAGGGACTA
TCAATAATTTGAAAGCTGATATATCCAATGTAGAAGAGGTAGCTAAATTATACGGGTTGGACAATTTAACTTATGATGAA
AAAGAAAGGTTAAAAACGGCTAAAAAGAAAATAGATGTAGGTCCAATTTTCACTACATTATTGTCTAAGCGTGCGAACAT
AGGCTTCACAACAGGAGGGCATACAGGTGAGGATGTATTCTTATATTCATACGGTCCTCAAAAGCCATACGGTTTGATTC
AAAATACGGACGTTGCGAAGACGATGGCGAAAGCGATGGGCTTTAACTTAGAAAAAGTAACAAACAAATTATTTGTAGAA
AGTGAATCAGCCTTTAAGCAGAATGGTGCAACTGTAACAATAGATAAAACTGATGTAGCGAACCCTGTACTCATAGTAAA
ACGTAATAATGTAACAGCTCAATTATTTGTTAACAAAAATATAATTCGTATTAAGAATAAAGAGTATGAATTAGGAAGTG
TTGTTGTAGAAAGTAATGGAAAGGTTTACGTACCAGAAGAAGCAATTCATCTATTTAGAAAACATTCTCGTTAA

Upstream 100 bases:

>100_bases
AGAAGAATTGGGAACTAATAAAACATATATAGGTTGTGAAAAGTAAAAGGAAACCAGGACTTTGTATGTAAATGAATGTG
AAACAAATGGAGGCGCAATA

Downstream 100 bases:

>100_bases
AAGATTGCCAACCACTTACCTTAATTAGGAGGAGTGGTTGGCAATTTTTTGTTTATTAAACGTAAGCAATTTGTAAGGAG
TGAGTAAGAAAAAAGAGAAT

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MFVLGLKMKKLLLVGTMTLSMFCTVSMTNNHTVEAKVKKVERQPKNVIIMVMDGTSSSATTLARLYKGKPLALDEIVTGG
VRTYSAESAITDSAPAATALATGNKSNSGYVGVLPTIVSSPGLKPMKEEDKLRPVANVLEGAKRTGRATGIVATAEIQHA
TPAGFSAHHVNRNNFEVLAEQQVYQNIDVVLGGGKAALLPETDSGIRKDGGDLVKVIKNKGYDFVETKEALLGSKSNKIW
GSFSLNALAFDMDREATNPEQPTLSQMTEKAIQTLSKDKDGFFLFVEGSKPDWAAHVNDPIGMISDVLAFDNAVAEALKF
AKKDGNTMLIAVADHGNSGISIGNMNTTKGYNTTPVSAYIYPLKKAKMTLEGTINNLKADISNVEEVAKLYGLDNLTYDE
KERLKTAKKKIDVGPIFTTLLSKRANIGFTTGGHTGEDVFLYSYGPQKPYGLIQNTDVAKTMAKAMGFNLEKVTNKLFVE
SESAFKQNGATVTIDKTDVANPVLIVKRNNVTAQLFVNKNIIRIKNKEYELGSVVVESNGKVYVPEEAIHLFRKHSR

Sequences:

>Translated_557_residues
MFVLGLKMKKLLLVGTMTLSMFCTVSMTNNHTVEAKVKKVERQPKNVIIMVMDGTSSSATTLARLYKGKPLALDEIVTGG
VRTYSAESAITDSAPAATALATGNKSNSGYVGVLPTIVSSPGLKPMKEEDKLRPVANVLEGAKRTGRATGIVATAEIQHA
TPAGFSAHHVNRNNFEVLAEQQVYQNIDVVLGGGKAALLPETDSGIRKDGGDLVKVIKNKGYDFVETKEALLGSKSNKIW
GSFSLNALAFDMDREATNPEQPTLSQMTEKAIQTLSKDKDGFFLFVEGSKPDWAAHVNDPIGMISDVLAFDNAVAEALKF
AKKDGNTMLIAVADHGNSGISIGNMNTTKGYNTTPVSAYIYPLKKAKMTLEGTINNLKADISNVEEVAKLYGLDNLTYDE
KERLKTAKKKIDVGPIFTTLLSKRANIGFTTGGHTGEDVFLYSYGPQKPYGLIQNTDVAKTMAKAMGFNLEKVTNKLFVE
SESAFKQNGATVTIDKTDVANPVLIVKRNNVTAQLFVNKNIIRIKNKEYELGSVVVESNGKVYVPEEAIHLFRKHSR
>Mature_557_residues
MFVLGLKMKKLLLVGTMTLSMFCTVSMTNNHTVEAKVKKVERQPKNVIIMVMDGTSSSATTLARLYKGKPLALDEIVTGG
VRTYSAESAITDSAPAATALATGNKSNSGYVGVLPTIVSSPGLKPMKEEDKLRPVANVLEGAKRTGRATGIVATAEIQHA
TPAGFSAHHVNRNNFEVLAEQQVYQNIDVVLGGGKAALLPETDSGIRKDGGDLVKVIKNKGYDFVETKEALLGSKSNKIW
GSFSLNALAFDMDREATNPEQPTLSQMTEKAIQTLSKDKDGFFLFVEGSKPDWAAHVNDPIGMISDVLAFDNAVAEALKF
AKKDGNTMLIAVADHGNSGISIGNMNTTKGYNTTPVSAYIYPLKKAKMTLEGTINNLKADISNVEEVAKLYGLDNLTYDE
KERLKTAKKKIDVGPIFTTLLSKRANIGFTTGGHTGEDVFLYSYGPQKPYGLIQNTDVAKTMAKAMGFNLEKVTNKLFVE
SESAFKQNGATVTIDKTDVANPVLIVKRNNVTAQLFVNKNIIRIKNKEYELGSVVVESNGKVYVPEEAIHLFRKHSR

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI94721246, Length=476, Percent_Identity=27.5210084033613, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI157266292, Length=483, Percent_Identity=25.6728778467909, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI157266296, Length=476, Percent_Identity=27.3109243697479, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI116734717, Length=338, Percent_Identity=29.585798816568, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI294660770, Length=334, Percent_Identity=28.7425149700599, Blast_Score=109, Evalue=8e-24,
Organism=Homo sapiens, GI294660772, Length=290, Percent_Identity=29.6551724137931, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI48994877, Length=365, Percent_Identity=31.7808219178082, Blast_Score=136, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6320689, Length=526, Percent_Identity=29.8479087452471, Blast_Score=178, Evalue=2e-45,
Organism=Drosophila melanogaster, GI85725200, Length=351, Percent_Identity=29.9145299145299, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI85815833, Length=351, Percent_Identity=29.9145299145299, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24657827, Length=471, Percent_Identity=28.6624203821656, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21355981, Length=327, Percent_Identity=31.1926605504587, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI19921912, Length=481, Percent_Identity=27.8586278586279, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24657835, Length=471, Percent_Identity=28.2377919320594, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI21355149, Length=339, Percent_Identity=29.7935103244838, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24657842, Length=471, Percent_Identity=27.6008492569002, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI18859923, Length=430, Percent_Identity=26.9767441860465, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24585213, Length=342, Percent_Identity=30.4093567251462, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21355151, Length=330, Percent_Identity=29.3939393939394, Blast_Score=116, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24651554, Length=476, Percent_Identity=26.0504201680672, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21358067, Length=340, Percent_Identity=28.2352941176471, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24651556, Length=454, Percent_Identity=25.9911894273128, Blast_Score=98, Evalue=2e-20,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 60383; Mature: 60383

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVLGLKMKKLLLVGTMTLSMFCTVSMTNNHTVEAKVKKVERQPKNVIIMVMDGTSSSAT
CEEECCCHHHHHHHHHEEEEEEEEEEECCCCEEHHHHHHHHCCCCCEEEEEECCCCCHHH
TLARLYKGKPLALDEIVTGGVRTYSAESAITDSAPAATALATGNKSNSGYVGVLPTIVSS
HHHHHHCCCCCCHHHHHCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCEEEEHHHHHCC
PGLKPMKEEDKLRPVANVLEGAKRTGRATGIVATAEIQHATPAGFSAHHVNRNNFEVLAE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEECCCCCHHEEEH
QQVYQNIDVVLGGGKAALLPETDSGIRKDGGDLVKVIKNKGYDFVETKEALLGSKSNKIW
HHHHCCCEEEEECCCEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCEE
GSFSLNALAFDMDREATNPEQPTLSQMTEKAIQTLSKDKDGFFLFVEGSKPDWAAHVNDP
EEEECEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEECCCC
IGMISDVLAFDNAVAEALKFAKKDGNTMLIAVADHGNSGISIGNMNTTKGYNTTPVSAYI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEECCCCCCCCCCCCCCEEEE
YPLKKAKMTLEGTINNLKADISNVEEVAKLYGLDNLTYDEKERLKTAKKKIDVGPIFTTL
EEHHHCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHH
LSKRANIGFTTGGHTGEDVFLYSYGPQKPYGLIQNTDVAKTMAKAMGFNLEKVTNKLFVE
HHCCCCCCEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHEEC
SESAFKQNGATVTIDKTDVANPVLIVKRNNVTAQLFVNKNIIRIKNKEYELGSVVVESNG
CCHHHHCCCCEEEEECCCCCCCEEEEEECCEEEEEEEECCEEEEECCCEEECEEEEECCC
KVYVPEEAIHLFRKHSR
CEECCHHHHHHHHHCCC
>Mature Secondary Structure
MFVLGLKMKKLLLVGTMTLSMFCTVSMTNNHTVEAKVKKVERQPKNVIIMVMDGTSSSAT
CEEECCCHHHHHHHHHEEEEEEEEEEECCCCEEHHHHHHHHCCCCCEEEEEECCCCCHHH
TLARLYKGKPLALDEIVTGGVRTYSAESAITDSAPAATALATGNKSNSGYVGVLPTIVSS
HHHHHHCCCCCCHHHHHCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCEEEEHHHHHCC
PGLKPMKEEDKLRPVANVLEGAKRTGRATGIVATAEIQHATPAGFSAHHVNRNNFEVLAE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEECCCCCHHEEEH
QQVYQNIDVVLGGGKAALLPETDSGIRKDGGDLVKVIKNKGYDFVETKEALLGSKSNKIW
HHHHCCCEEEEECCCEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCEE
GSFSLNALAFDMDREATNPEQPTLSQMTEKAIQTLSKDKDGFFLFVEGSKPDWAAHVNDP
EEEECEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEECCCC
IGMISDVLAFDNAVAEALKFAKKDGNTMLIAVADHGNSGISIGNMNTTKGYNTTPVSAYI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEECCCCCCCCCCCCCCEEEE
YPLKKAKMTLEGTINNLKADISNVEEVAKLYGLDNLTYDEKERLKTAKKKIDVGPIFTTL
EEHHHCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHH
LSKRANIGFTTGGHTGEDVFLYSYGPQKPYGLIQNTDVAKTMAKAMGFNLEKVTNKLFVE
HHCCCCCCEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHEEC
SESAFKQNGATVTIDKTDVANPVLIVKRNNVTAQLFVNKNIIRIKNKEYELGSVVVESNG
CCHHHHCCCCEEEEECCCCCCCEEEEEECCEEEEEEEECCEEEEECCCEEECEEEEECCC
KVYVPEEAIHLFRKHSR
CEECCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]