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The map label for this gene is 52142471

Identifier: 52142471

GI number: 52142471

Start: 2889122

End: 2890147

Strand: Reverse

Name: 52142471

Synonym: BCZK2770

Alternate gene names: NA

Gene position: 2890147-2889122 (Counterclockwise)

Preceding gene: 52142472

Following gene: 52142473

Centisome position: 54.52

GC content: 38.4

Gene sequence:

>1026_bases
ATGAAAATTCTAATACTAGGTGGTACACGATTTTTGGGAAGAGCTGTGGTAGAAGAGGCTTTGAACAGAGGACATGAAGT
TACATTATTTAACCGTGGAACAAACAAGGAAATTTTTCCTGAAGTGGAGCAGCTTATCGGTGACAGAAATGGTGATGTAT
CAAGTTTAGAAAATCGTAAATGGGACGTTGTCATAGATACATGTGGATTTTCTCCACATCACATAAGAAATGTTGGAGAA
GTACTTAAAGATAATATTGAACACTATATATTCATCTCAAGCCTTTCCGTATATAAAGATTGGATTCCGCATCATATAAA
AGAAGACTATATATTACAACCTGAACCAACGAAAGAGCAAATAAAGGCTGTAGAAAATGGTGAAATATCTCCTTATGAGC
ATTATGGTGCGCTGAAAGTATTATGCGAAAAAGAAGCAGAGAAGTATTGGCCGCGGCGTGTTTTACACGTAAGAGCAGGA
CTTCTTTCAGGAATGTTTGATTATACAGATCGACTTCCATACTGGATTGGGCGTGTAGCAAAGGGAGGAAAAGTGTTAGT
ACCGGGAAGAAAAAATCGTCCAGTGCAGATAGTTGATATAAAAGACGTCGCAAACTGGGGACTAAATATGGTAGAAAACA
ATAAAGCAGGTATATTCAATGCTACAGGTCCAAATTATGAATTGACGATGGAAGAGCTATTAAATACGTGTAAAAAAGTT
ACGAATAGTGATGCTGAATTTGTTTGGATAGAGGAATCGTTCATGAATGAACATAAAGTGCAGCCTTGGACAGAGATGCC
TTTATGGCTTCCAGAAACTTTTCCGTTAGAAGGGGAAACGAAGCCGTGGAAAGGTGGGTTTTCTATTAGTATCGAGAGTG
CTATTAAAGTGGGGCTTACTTTTAGAAGGTTAGAAGAGACAGTTACAGACGTGTATGAGTGGATGGAGAGTACGGACGAA
TGGGAATTAAAAGCAGGTATTTCAGGCGAGAGGGAGAAGGAATTGTTAGAAAAGTGGTATCAATAA

Upstream 100 bases:

>100_bases
TTGTAGGATTAAAGTAAAATAACAAATCCACCCGTAACTTTTTATGATCGCAATTCGTTTAACAAAAGAAAGGATTTCTA
ATGATGAATGGGGGAGAACC

Downstream 100 bases:

>100_bases
ATTTAGCGGAAAAATAACAATTTAAACCTAGTATTAAGAAAAAACGCCTTGTTACTAAAGAAACAAGGTGTTTTTCGTAT
GTGAATTTATAATTCAATTG

Product: isoflavone reductase

Products: 2'-hydroxyformononetin; NADPH; H+

Alternate protein names: Reductase; Isoflavone Reductase; NAD Dependent Epimerase/Dehydratase Family Protein; Nucleoside-Diphosphate-Sugar Epimerase; NAD-Dependent Epimerase/Dehydratase Family Protein; NAD Dependent Epimerase/Dehydratase; 3-Beta Hydroxysteroid Dehydrogenase/Isomerase Family; Nucleotide Sugar Epimerase; 3-Beta Hydroxysteroid Dehydrogenase/Isomerase; Oxidoreductase; DTDP-Glucose 4 6-Dehydratase; NmrA Family Protein; Sugar Dehydratase; Dtdpglucose 4 6-Dehydratase-Like Protein; NAD-Dependent Protein; NAD Dependent Epimerase; NAD Dependent Epimerase/Dehydratase Family

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKILILGGTRFLGRAVVEEALNRGHEVTLFNRGTNKEIFPEVEQLIGDRNGDVSSLENRKWDVVIDTCGFSPHHIRNVGE
VLKDNIEHYIFISSLSVYKDWIPHHIKEDYILQPEPTKEQIKAVENGEISPYEHYGALKVLCEKEAEKYWPRRVLHVRAG
LLSGMFDYTDRLPYWIGRVAKGGKVLVPGRKNRPVQIVDIKDVANWGLNMVENNKAGIFNATGPNYELTMEELLNTCKKV
TNSDAEFVWIEESFMNEHKVQPWTEMPLWLPETFPLEGETKPWKGGFSISIESAIKVGLTFRRLEETVTDVYEWMESTDE
WELKAGISGEREKELLEKWYQ

Sequences:

>Translated_341_residues
MKILILGGTRFLGRAVVEEALNRGHEVTLFNRGTNKEIFPEVEQLIGDRNGDVSSLENRKWDVVIDTCGFSPHHIRNVGE
VLKDNIEHYIFISSLSVYKDWIPHHIKEDYILQPEPTKEQIKAVENGEISPYEHYGALKVLCEKEAEKYWPRRVLHVRAG
LLSGMFDYTDRLPYWIGRVAKGGKVLVPGRKNRPVQIVDIKDVANWGLNMVENNKAGIFNATGPNYELTMEELLNTCKKV
TNSDAEFVWIEESFMNEHKVQPWTEMPLWLPETFPLEGETKPWKGGFSISIESAIKVGLTFRRLEETVTDVYEWMESTDE
WELKAGISGEREKELLEKWYQ
>Mature_341_residues
MKILILGGTRFLGRAVVEEALNRGHEVTLFNRGTNKEIFPEVEQLIGDRNGDVSSLENRKWDVVIDTCGFSPHHIRNVGE
VLKDNIEHYIFISSLSVYKDWIPHHIKEDYILQPEPTKEQIKAVENGEISPYEHYGALKVLCEKEAEKYWPRRVLHVRAG
LLSGMFDYTDRLPYWIGRVAKGGKVLVPGRKNRPVQIVDIKDVANWGLNMVENNKAGIFNATGPNYELTMEELLNTCKKV
TNSDAEFVWIEESFMNEHKVQPWTEMPLWLPETFPLEGETKPWKGGFSISIESAIKVGLTFRRLEETVTDVYEWMESTDE
WELKAGISGEREKELLEKWYQ

Specific function: Unknown

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.3.1.45

Molecular weight: Translated: 39410; Mature: 39410

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILILGGTRFLGRAVVEEALNRGHEVTLFNRGTNKEIFPEVEQLIGDRNGDVSSLENRK
CEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHCCCC
WDVVIDTCGFSPHHIRNVGEVLKDNIEHYIFISSLSVYKDWIPHHIKEDYILQPEPTKEQ
EEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCCCCEECCCCCHHH
IKAVENGEISPYEHYGALKVLCEKEAEKYWPRRVLHVRAGLLSGMFDYTDRLPYWIGRVA
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
KGGKVLVPGRKNRPVQIVDIKDVANWGLNMVENNKAGIFNATGPNYELTMEELLNTCKKV
CCCEEEECCCCCCCEEEEEHHHHHHCCCEEEECCCCCEEECCCCCCEEEHHHHHHHHHHH
TNSDAEFVWIEESFMNEHKVQPWTEMPLWLPETFPLEGETKPWKGGFSISIESAIKVGLT
CCCCCCEEEEEHHHCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHH
FRRLEETVTDVYEWMESTDEWELKAGISGEREKELLEKWYQ
HHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKILILGGTRFLGRAVVEEALNRGHEVTLFNRGTNKEIFPEVEQLIGDRNGDVSSLENRK
CEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHCCCC
WDVVIDTCGFSPHHIRNVGEVLKDNIEHYIFISSLSVYKDWIPHHIKEDYILQPEPTKEQ
EEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCCCCEECCCCCHHH
IKAVENGEISPYEHYGALKVLCEKEAEKYWPRRVLHVRAGLLSGMFDYTDRLPYWIGRVA
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
KGGKVLVPGRKNRPVQIVDIKDVANWGLNMVENNKAGIFNATGPNYELTMEELLNTCKKV
CCCEEEECCCCCCCEEEEEHHHHHHCCCEEEECCCCCEEECCCCCCEEEHHHHHHHHHHH
TNSDAEFVWIEESFMNEHKVQPWTEMPLWLPETFPLEGETKPWKGGFSISIESAIKVGLT
CCCCCCEEEEEHHHCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHH
FRRLEETVTDVYEWMESTDEWELKAGISGEREKELLEKWYQ
HHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vestitone; NADP+

Specific reaction: vestitone + NADP+ = 2'-hydroxyformononetin + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA