Click here to switch to the map view.

The map label for this gene is ykgF [H]

Identifier: 52142285

GI number: 52142285

Start: 3089916

End: 3091328

Strand: Reverse

Name: ykgF [H]

Synonym: BCZK2957

Alternate gene names: 52142285

Gene position: 3091328-3089916 (Counterclockwise)

Preceding gene: 52142284

Following gene: 52142286

Centisome position: 58.32

GC content: 39.7

Gene sequence:

>1413_bases
ATGAGTGTGGCGGGGGAACTAGATTTTTTGAAAAGAGTAGATAAAGCGTTGCATGATCCGCAGATGAGAAGCGCGGTTCC
GTTTACACAAGATCGGCTTCGGATGGGACGGGAACAGGCTACACATGAATTAGGGAATATGGAAGAATGGCGCGAGGCAG
CATCTGAAATTCGGCTGCATACAATCGGAAATTTAGATAGTTATTTGGAACAATTAGCTACTAATGTTCGGAAAAATGGG
GGGCATGTGCATTTCGCTTTCAAAGCAGAAGAGGCTGTATCATATGTTTCTGAAATCGTAAGAAAAAAAGGAGCGAGATC
AGTAATTAAGTCAAAATCCATGGTTTCAGAAGAAATACATCTTAATAAAAACTTAGAAGAGATGGGGGTAAAAGTAGTAG
AGTCTGATCTTGGGGAGTATATTATTCAATTAGCTGGAGAAACGCCATCCCATTTAATCGCCCCGGCCATTCATAAGACG
AAGGAACAAGTAGCAGAGCTTTTTTCTAAGGAAGCAGGAAGAAAAATCCCAGTTGATACGAATCAATTACTACAATTTGC
AAGGGAGAAATTGCGTAAAGAGTTTTTAGAAGCTGATATTGGTATTTCCGGTTGTAACTTTGCGGTAGCGGAATCTGGAT
CAGTTGTGCTTATAAGTAATGAAGGAAATGCTCGCCTTACTACGACATTGCCAAAGGTTCATATTGCGATTATGGGGATG
GAACGCCTTGTTCCGACATGGAAAGATCTTGATGTAGTCATTTCGATGTTAACAAGAAGTGCTACAGGCCAAAAGATTAC
ATCTTATGTAACTGGAATTAATGGGGCGCGTTTACATGAAGATATGGATGGACCTGAAGAATTTCATTTAATTATTTTAG
ATAATGGCCGCTCTGATATTTTAGGAACTGCTTATGAAGAAGTATTAAAGTGTATAAGGTGCGGAGCTTGTGTAAATGTT
TGTCCTGTGTATCGCAATATTGGAGGCCATGCTTATGGCAGTGTGTATAGCGGGCCAATTGGAGCAGTACTTGCGCCTTT
ATTAGAAGGATATGAAGAGTTAAAAGAATTACCTTTTGCATCCAGTTTGTGCGGAGCATGTACAGATGTTTGCCCTGTGA
AAATTCCATTACATGACCTTCTCATTGAACATCGTAAAGATGTCGTGGAACAAGGGTTTATAACGAAATTTGAGCAACTT
GCCTTTAAAGGTTTCACGTTTATGGTGAGTCATCCATTCGTTTATGAGAAAGCAATTAAAATGGCACATACAGGGTTATC
GATATTTGCAAAAGATGGTTATATTGCGAATGGTCCAGGTCCATTAAAAGGATGGACAGAAAGTAGAGATTTTCCAAAAC
CTGCGAAACAATCATTCCGTGATTGGTGGAAAAAAGAAACGAGGGATTCATAA

Upstream 100 bases:

>100_bases
ATATGGGATGTCTTATGAATATTGGGGGAAGATTGCGCAGGAAAAATAAAAATATTCAAGTGTTACATGTAGCAGAAGTA
CTGGCGAAGGGGCTGAATAA

Downstream 100 bases:

>100_bases
TATGAATACGGATGTACAAGAGTCTTTTTTAAACAATATCGCACGTAAATTAAATCGAGAACGTCGCTCGGGAGTTACTC
CTCCCAGGTGGAAAAACAAT

Product: iron-sulfur cluster-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSVAGELDFLKRVDKALHDPQMRSAVPFTQDRLRMGREQATHELGNMEEWREAASEIRLHTIGNLDSYLEQLATNVRKNG
GHVHFAFKAEEAVSYVSEIVRKKGARSVIKSKSMVSEEIHLNKNLEEMGVKVVESDLGEYIIQLAGETPSHLIAPAIHKT
KEQVAELFSKEAGRKIPVDTNQLLQFAREKLRKEFLEADIGISGCNFAVAESGSVVLISNEGNARLTTTLPKVHIAIMGM
ERLVPTWKDLDVVISMLTRSATGQKITSYVTGINGARLHEDMDGPEEFHLIILDNGRSDILGTAYEEVLKCIRCGACVNV
CPVYRNIGGHAYGSVYSGPIGAVLAPLLEGYEELKELPFASSLCGACTDVCPVKIPLHDLLIEHRKDVVEQGFITKFEQL
AFKGFTFMVSHPFVYEKAIKMAHTGLSIFAKDGYIANGPGPLKGWTESRDFPKPAKQSFRDWWKKETRDS

Sequences:

>Translated_470_residues
MSVAGELDFLKRVDKALHDPQMRSAVPFTQDRLRMGREQATHELGNMEEWREAASEIRLHTIGNLDSYLEQLATNVRKNG
GHVHFAFKAEEAVSYVSEIVRKKGARSVIKSKSMVSEEIHLNKNLEEMGVKVVESDLGEYIIQLAGETPSHLIAPAIHKT
KEQVAELFSKEAGRKIPVDTNQLLQFAREKLRKEFLEADIGISGCNFAVAESGSVVLISNEGNARLTTTLPKVHIAIMGM
ERLVPTWKDLDVVISMLTRSATGQKITSYVTGINGARLHEDMDGPEEFHLIILDNGRSDILGTAYEEVLKCIRCGACVNV
CPVYRNIGGHAYGSVYSGPIGAVLAPLLEGYEELKELPFASSLCGACTDVCPVKIPLHDLLIEHRKDVVEQGFITKFEQL
AFKGFTFMVSHPFVYEKAIKMAHTGLSIFAKDGYIANGPGPLKGWTESRDFPKPAKQSFRDWWKKETRDS
>Mature_469_residues
SVAGELDFLKRVDKALHDPQMRSAVPFTQDRLRMGREQATHELGNMEEWREAASEIRLHTIGNLDSYLEQLATNVRKNGG
HVHFAFKAEEAVSYVSEIVRKKGARSVIKSKSMVSEEIHLNKNLEEMGVKVVESDLGEYIIQLAGETPSHLIAPAIHKTK
EQVAELFSKEAGRKIPVDTNQLLQFAREKLRKEFLEADIGISGCNFAVAESGSVVLISNEGNARLTTTLPKVHIAIMGME
RLVPTWKDLDVVISMLTRSATGQKITSYVTGINGARLHEDMDGPEEFHLIILDNGRSDILGTAYEEVLKCIRCGACVNVC
PVYRNIGGHAYGSVYSGPIGAVLAPLLEGYEELKELPFASSLCGACTDVCPVKIPLHDLLIEHRKDVVEQGFITKFEQLA
FKGFTFMVSHPFVYEKAIKMAHTGLSIFAKDGYIANGPGPLKGWTESRDFPKPAKQSFRDWWKKETRDS

Specific function: Is involved in L-lactate degradation and allows cells to grow with lactate as the sole carbon source. Has probably a role as an electron transporter during oxidation of L-lactate [H]

COG id: COG1139

COG function: function code C; Uncharacterized conserved protein containing a ferredoxin-like domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1786498, Length=458, Percent_Identity=49.7816593886463, Blast_Score=505, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017900
- InterPro:   IPR003741
- InterPro:   IPR004452
- InterPro:   IPR002698
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR022825 [H]

Pfam domain/function: PF02589 DUF162 [H]

EC number: NA

Molecular weight: Translated: 52242; Mature: 52110

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAGELDFLKRVDKALHDPQMRSAVPFTQDRLRMGREQATHELGNMEEWREAASEIRLH
CCCCHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TIGNLDSYLEQLATNVRKNGGHVHFAFKAEEAVSYVSEIVRKKGARSVIKSKSMVSEEIH
HCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNKNLEEMGVKVVESDLGEYIIQLAGETPSHLIAPAIHKTKEQVAELFSKEAGRKIPVDT
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
NQLLQFAREKLRKEFLEADIGISGCNFAVAESGSVVLISNEGNARLTTTLPKVHIAIMGM
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCEEEEECCHHHHHHHHH
ERLVPTWKDLDVVISMLTRSATGQKITSYVTGINGARLHEDMDGPEEFHLIILDNGRSDI
HHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHCCCCCCCCCEEEEEEECCCCCH
LGTAYEEVLKCIRCGACVNVCPVYRNIGGHAYGSVYSGPIGAVLAPLLEGYEELKELPFA
HHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCH
SSLCGACTDVCPVKIPLHDLLIEHRKDVVEQGFITKFEQLAFKGFTFMVSHPFVYEKAIK
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHH
MAHTGLSIFAKDGYIANGPGPLKGWTESRDFPKPAKQSFRDWWKKETRDS
HHHCCCEEEECCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SVAGELDFLKRVDKALHDPQMRSAVPFTQDRLRMGREQATHELGNMEEWREAASEIRLH
CCCHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TIGNLDSYLEQLATNVRKNGGHVHFAFKAEEAVSYVSEIVRKKGARSVIKSKSMVSEEIH
HCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNKNLEEMGVKVVESDLGEYIIQLAGETPSHLIAPAIHKTKEQVAELFSKEAGRKIPVDT
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
NQLLQFAREKLRKEFLEADIGISGCNFAVAESGSVVLISNEGNARLTTTLPKVHIAIMGM
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCEEEEECCHHHHHHHHH
ERLVPTWKDLDVVISMLTRSATGQKITSYVTGINGARLHEDMDGPEEFHLIILDNGRSDI
HHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHCCCCCCCCCEEEEEEECCCCCH
LGTAYEEVLKCIRCGACVNVCPVYRNIGGHAYGSVYSGPIGAVLAPLLEGYEELKELPFA
HHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCH
SSLCGACTDVCPVKIPLHDLLIEHRKDVVEQGFITKFEQLAFKGFTFMVSHPFVYEKAIK
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHH
MAHTGLSIFAKDGYIANGPGPLKGWTESRDFPKPAKQSFRDWWKKETRDS
HHHCCCEEEECCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA