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The map label for this gene is yfkO [H]

Identifier: 52142212

GI number: 52142212

Start: 3160323

End: 3161006

Strand: Direct

Name: yfkO [H]

Synonym: BCZK3028

Alternate gene names: 52142212

Gene position: 3160323-3161006 (Clockwise)

Preceding gene: 52142216

Following gene: 52142213

Centisome position: 59.62

GC content: 33.33

Gene sequence:

>684_bases
ATGACAAATACAAATCAAATTACAAAAGAAAAAATTATGGAGGCTTATCATTTCAGACATGCATGTAAAGAGTTCGATTC
TATGCAAAAAATTTCAGAAGAGGATTTTAAATTTATTTTAGAAACAGGAAGATTATCTCCTTCTTCTTTCGGATATGAAC
CTTGGAAATTTGTTGTCGTACAAAATAAAGAATTAAGAGAAAAACTACGACCATATTCATGGGGCGCTGGTGGGCAACTT
GCAACAGCTAGCCACTTTGTCATTGTTCTTTCAAGAAATATTAAAGATATGCATTATGATGCAGATTATATTAAACATAT
GATAAATGATATTATTGAATTACCTGAAGACGCGCAAAAAATCAGATATGAATTCTTCAAAAAGTTCCAGGAGTCAGATT
TTAACTTATTACAATCCGACCGTGCTGTATTCGATTGGGCATCTAAGCAAACTTATATTGCTTTAGGGAATATGATGACG
AGCGCAGCTCAAATCGGGATTGATTCTTGTCCAATAGAAGGATTTGATAAAGAGAAAGTAGATTCTTTACTTCGTCAAGA
AGGCATAATCAATGGAGATAACTTTGAAGTATCTGTTATGGTTGCCTTTGGTTACCGTAAAGAAGAGCCAAAACGTGATA
AAACAAGACAGACTATGGATGCAATTGTTGAATGGATTGAATAA

Upstream 100 bases:

>100_bases
CTTTTTTGATACTATACCACATAAATGTGCGTACTTGTGTCTATGTTACTTTAAAAATATAATTTACTTACAAAATAAAT
AAATGAAAAGAGGAACTTTT

Downstream 100 bases:

>100_bases
GATAACATGCGCTCCTTATAGCTGTATACTCATACGAAAAAAGTAGAATTTCATTTTTCTTACGTATGAAATTAGTTTTT
CATGGAAAGATATATTAGTA

Product: NAD(P)H-flavin oxidoreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MTNTNQITKEKIMEAYHFRHACKEFDSMQKISEEDFKFILETGRLSPSSFGYEPWKFVVVQNKELREKLRPYSWGAGGQL
ATASHFVIVLSRNIKDMHYDADYIKHMINDIIELPEDAQKIRYEFFKKFQESDFNLLQSDRAVFDWASKQTYIALGNMMT
SAAQIGIDSCPIEGFDKEKVDSLLRQEGIINGDNFEVSVMVAFGYRKEEPKRDKTRQTMDAIVEWIE

Sequences:

>Translated_227_residues
MTNTNQITKEKIMEAYHFRHACKEFDSMQKISEEDFKFILETGRLSPSSFGYEPWKFVVVQNKELREKLRPYSWGAGGQL
ATASHFVIVLSRNIKDMHYDADYIKHMINDIIELPEDAQKIRYEFFKKFQESDFNLLQSDRAVFDWASKQTYIALGNMMT
SAAQIGIDSCPIEGFDKEKVDSLLRQEGIINGDNFEVSVMVAFGYRKEEPKRDKTRQTMDAIVEWIE
>Mature_226_residues
TNTNQITKEKIMEAYHFRHACKEFDSMQKISEEDFKFILETGRLSPSSFGYEPWKFVVVQNKELREKLRPYSWGAGGQLA
TASHFVIVLSRNIKDMHYDADYIKHMINDIIELPEDAQKIRYEFFKKFQESDFNLLQSDRAVFDWASKQTYIALGNMMTS
AAQIGIDSCPIEGFDKEKVDSLLRQEGIINGDNFEVSVMVAFGYRKEEPKRDKTRQTMDAIVEWIE

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=177, Percent_Identity=28.8135593220339, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 26511; Mature: 26380

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTNQITKEKIMEAYHFRHACKEFDSMQKISEEDFKFILETGRLSPSSFGYEPWKFVVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
QNKELREKLRPYSWGAGGQLATASHFVIVLSRNIKDMHYDADYIKHMINDIIELPEDAQK
CCHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHCCCHHHHHHHHHHHHHCCHHHHH
IRYEFFKKFQESDFNLLQSDRAVFDWASKQTYIALGNMMTSAAQIGIDSCPIEGFDKEKV
HHHHHHHHHHHCCHHHHHHCHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCHHHH
DSLLRQEGIINGDNFEVSVMVAFGYRKEEPKRDKTRQTMDAIVEWIE
HHHHHHCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TNTNQITKEKIMEAYHFRHACKEFDSMQKISEEDFKFILETGRLSPSSFGYEPWKFVVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
QNKELREKLRPYSWGAGGQLATASHFVIVLSRNIKDMHYDADYIKHMINDIIELPEDAQK
CCHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHCCCHHHHHHHHHHHHHCCHHHHH
IRYEFFKKFQESDFNLLQSDRAVFDWASKQTYIALGNMMTSAAQIGIDSCPIEGFDKEKV
HHHHHHHHHHHCCHHHHHHCHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCHHHH
DSLLRQEGIINGDNFEVSVMVAFGYRKEEPKRDKTRQTMDAIVEWIE
HHHHHHCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]