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The map label for this gene is bla [H]

Identifier: 52142035

GI number: 52142035

Start: 3331050

End: 3332183

Strand: Reverse

Name: bla [H]

Synonym: BCZK3208

Alternate gene names: 52142035

Gene position: 3332183-3331050 (Counterclockwise)

Preceding gene: 52142033

Following gene: 52142038

Centisome position: 62.86

GC content: 33.6

Gene sequence:

>1134_bases
ATGTTAAAGAAATGGTTAATCGTTTTCTTTATTTTTGCTGTTTTTTCTGGAAGTGTCGTTGCACTTATACATGCAAGTAA
AGGTAAGAAATATGATATAAAGGCATTTTCTAATTTGAATAATAAGCAAAAGCATGATATGCAAGAAGTTGATGCCAATG
AGAAAAAGCACTATGAAGTTGTAGCTGGAAAACTAGATCAATATTTAAAAGATAAAGGATTTAATGGTAGTGTTCTTGTA
GCTAGTAAAGACCATGTGATTTTACAAAAAGGTTATGGGTATGCAAATGTGAAAGATAAAGTATTCACAACGCCAAGAAC
GAAATATCGCATTGGTTCTATTACGAAAACAGTCGTTGCAATATCTATTATGCAACTAAAAGAAAAAGGAAAATTAAATA
TTGAAGACAATGTAAATAAGTATATTCCATCGTTTCCCGCAGATAAAAATATTACGTTACGAAATTTATTAACGCACACA
TCAGGGTTACCAGATCAAGGAAAAGGTAGGGTGGATGCGGCGTCACGTTTAAAGTTAGTAACGTGGATTGGTTCGCAAGC
GTTACAATTTCCTGCAGGGACAGGATGGAAATATACAGACTATAATTATATGGTGCTTGCGTATATTGTAGAAAAAATTA
CGAATAAACCGCTCGCTGAATACGTGAAAGAAAATATTTTCACTCCTGTTGAAATGCATGAATCTGGAATGGGGGCAACT
CTCCCAGAAGATATTTTTTTAGCAGAAGGTTATACGAGAAAAGATAATGAATTAATAGCTGCACCCCGCTTAAAAATGAA
CTGGTTGTATGGATGTGGTGAAATGTATACGACTGTTGAAGATATGAAAAGGCTAGATGAGGCTATTATGAATGGAAAAC
TGTTATCTAAACAAAGTGTATTAGATATGTTTACTGCATCACCTGCAAGAAAATATGGATTTAGTTTCTATACGTATCCA
GATTATTATCATAACCACGGGGTATTAGCAGGCTGGAATACTTTTAATAATTTTAACTGGGATAAAAGAACGTTTGTCAT
ATTGTTCTCTAACGTCCAAAATGGTATGAATGATGCATTTAATCAAGAGTTTAGGAAAATGGCGAATGATTTAATAGAAG
GAAAGGGAGCATGA

Upstream 100 bases:

>100_bases
AGGTGAATAATAGGTAGTATTATAAAAAATTTAAAAATTAATAGATATAGAATAGAATGGATGCGCGTTAAGTGAAACTA
GATAAAAGAGGGTTCCTATT

Downstream 100 bases:

>100_bases
AAGAGAAAGAGCCTCTAGCGAGAGGCTCTTCTTTTTTATAAAGTTGAAAACTCTTCAACAAGTTCACTAAAACGATTTAA
TGCACTTTCAATCGGTTCTG

Product: beta-lactamase

Products: substituted beta-amino acid

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MLKKWLIVFFIFAVFSGSVVALIHASKGKKYDIKAFSNLNNKQKHDMQEVDANEKKHYEVVAGKLDQYLKDKGFNGSVLV
ASKDHVILQKGYGYANVKDKVFTTPRTKYRIGSITKTVVAISIMQLKEKGKLNIEDNVNKYIPSFPADKNITLRNLLTHT
SGLPDQGKGRVDAASRLKLVTWIGSQALQFPAGTGWKYTDYNYMVLAYIVEKITNKPLAEYVKENIFTPVEMHESGMGAT
LPEDIFLAEGYTRKDNELIAAPRLKMNWLYGCGEMYTTVEDMKRLDEAIMNGKLLSKQSVLDMFTASPARKYGFSFYTYP
DYYHNHGVLAGWNTFNNFNWDKRTFVILFSNVQNGMNDAFNQEFRKMANDLIEGKGA

Sequences:

>Translated_377_residues
MLKKWLIVFFIFAVFSGSVVALIHASKGKKYDIKAFSNLNNKQKHDMQEVDANEKKHYEVVAGKLDQYLKDKGFNGSVLV
ASKDHVILQKGYGYANVKDKVFTTPRTKYRIGSITKTVVAISIMQLKEKGKLNIEDNVNKYIPSFPADKNITLRNLLTHT
SGLPDQGKGRVDAASRLKLVTWIGSQALQFPAGTGWKYTDYNYMVLAYIVEKITNKPLAEYVKENIFTPVEMHESGMGAT
LPEDIFLAEGYTRKDNELIAAPRLKMNWLYGCGEMYTTVEDMKRLDEAIMNGKLLSKQSVLDMFTASPARKYGFSFYTYP
DYYHNHGVLAGWNTFNNFNWDKRTFVILFSNVQNGMNDAFNQEFRKMANDLIEGKGA
>Mature_377_residues
MLKKWLIVFFIFAVFSGSVVALIHASKGKKYDIKAFSNLNNKQKHDMQEVDANEKKHYEVVAGKLDQYLKDKGFNGSVLV
ASKDHVILQKGYGYANVKDKVFTTPRTKYRIGSITKTVVAISIMQLKEKGKLNIEDNVNKYIPSFPADKNITLRNLLTHT
SGLPDQGKGRVDAASRLKLVTWIGSQALQFPAGTGWKYTDYNYMVLAYIVEKITNKPLAEYVKENIFTPVEMHESGMGAT
LPEDIFLAEGYTRKDNELIAAPRLKMNWLYGCGEMYTTVEDMKRLDEAIMNGKLLSKQSVLDMFTASPARKYGFSFYTYP
DYYHNHGVLAGWNTFNNFNWDKRTFVILFSNVQNGMNDAFNQEFRKMANDLIEGKGA

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential). Note=Localizes to the division septum during vegetative growth, and accumulates at the prespore during sporulation [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI87082101, Length=327, Percent_Identity=22.9357798165138, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17550934, Length=278, Percent_Identity=24.1007194244604, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: 3.5.2.6

Molecular weight: Translated: 42877; Mature: 42877

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKWLIVFFIFAVFSGSVVALIHASKGKKYDIKAFSNLNNKQKHDMQEVDANEKKHYEV
CCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEHHHHCCCCHHHHHHHHHCCCCHHHHHH
VAGKLDQYLKDKGFNGSVLVASKDHVILQKGYGYANVKDKVFTTPRTKYRIGSITKTVVA
HHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCCCCCCCCEEECCCCCEEHHHHHHHHHH
ISIMQLKEKGKLNIEDNVNKYIPSFPADKNITLRNLLTHTSGLPDQGKGRVDAASRLKLV
HHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHEEEE
TWIGSQALQFPAGTGWKYTDYNYMVLAYIVEKITNKPLAEYVKENIFTPVEMHESGMGAT
EECCCHHEECCCCCCCEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCCCCCCC
LPEDIFLAEGYTRKDNELIAAPRLKMNWLYGCGEMYTTVEDMKRLDEAIMNGKLLSKQSV
CCCCEEEECCCCCCCCCEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
LDMFTASPARKYGFSFYTYPDYYHNHGVLAGWNTFNNFNWDKRTFVILFSNVQNGMNDAF
HHHHHCCCHHHHCCEEEECCCHHCCCCEEEECCCCCCCCCCCEEEEEEEECCCCCHHHHH
NQEFRKMANDLIEGKGA
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLKKWLIVFFIFAVFSGSVVALIHASKGKKYDIKAFSNLNNKQKHDMQEVDANEKKHYEV
CCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEHHHHCCCCHHHHHHHHHCCCCHHHHHH
VAGKLDQYLKDKGFNGSVLVASKDHVILQKGYGYANVKDKVFTTPRTKYRIGSITKTVVA
HHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCCCCCCCCEEECCCCCEEHHHHHHHHHH
ISIMQLKEKGKLNIEDNVNKYIPSFPADKNITLRNLLTHTSGLPDQGKGRVDAASRLKLV
HHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHEEEE
TWIGSQALQFPAGTGWKYTDYNYMVLAYIVEKITNKPLAEYVKENIFTPVEMHESGMGAT
EECCCHHEECCCCCCCEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCCCCCCC
LPEDIFLAEGYTRKDNELIAAPRLKMNWLYGCGEMYTTVEDMKRLDEAIMNGKLLSKQSV
CCCCEEEECCCCCCCCCEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
LDMFTASPARKYGFSFYTYPDYYHNHGVLAGWNTFNNFNWDKRTFVILFSNVQNGMNDAF
HHHHHCCCHHHHCCEEEECCCHHCCCCEEEECCCCCCCCCCCEEEEEEEECCCCCHHHHH
NQEFRKMANDLIEGKGA
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: beta-lactam; H2O

Specific reaction: A beta-lactam + H2O = a substituted beta-amino acid

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]