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The map label for this gene is nodB [H]

Identifier: 52141923

GI number: 52141923

Start: 3456206

End: 3456847

Strand: Reverse

Name: nodB [H]

Synonym: BCZK3321

Alternate gene names: 52141923

Gene position: 3456847-3456206 (Counterclockwise)

Preceding gene: 52141922

Following gene: 52141924

Centisome position: 65.21

GC content: 34.58

Gene sequence:

>642_bases
ATGTTGCTTAGAAAAGAGCTAGAACCAACTGGCTATGTAACATGGGAAGTACCTAATAATGAAAAGATTATTGCCATTAC
ATTTGATGATGGACCAGATCCAACTTATACACCACAAATTTTAAATTTATTACGTCAATATAAAGCAGAAGCGACATTTT
TTATGATCGGATTTCGAGTTCAGCGTAATCCATATTTAGTGAAACAAGTGTTAAAAGAAGGACATGAAATTGGAAATCAT
ACGATGAACCATTTGTATGCGAGTAATTCTTCAGATGAAAAATTAGAAAACGATATTTTAGATGGAAAGAAATTTTTCGA
AAAATGGGTGAAAGAACCTTTACTATTCCGTCCACCTGGCGGATATATTAACGATGCTGTATTTAAAACAGCGAAAGAAG
CTGGTTATCAAACTGTTCTATGGTCATGGCATCAAGATCCACGTGATTGGGCAAATCCAGGGGTTCAATCCATTGTAAAT
CATGTATTGAAAAATGCTAAAAGTGGCGATATTGTATTGTTACATGATGGAGGAAATAATCGTAGTCAAACAGTAGCAGC
GCTAGCAAAAATTTTACCTGAACTGAAAAAGCAGGGCTATCGATTTGTAACGGTTTCGGAATTATTACGTTATAAACATT
AG

Upstream 100 bases:

>100_bases
TACTTTAATAAATGTTATGTTAAGACAGAAAATTTTTCTATTCGTTTTTTCTTTATTATGCGCAGTACATATATTTCAAG
TTGAAAAAGTGGAAGCGAAA

Downstream 100 bases:

>100_bases
AAAAAATCCCAAAAGGTGAATGTTCCTTTTGGGATTTTTCGTTACTGGTTCTTTTTGCGTTTCTTATTATTTTGTCGTTC
TGCAGGAGTTAAGTTTTCAT

Product: polysaccharide deacetylase

Products: NA

Alternate protein names: Nodulation protein B [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MLLRKELEPTGYVTWEVPNNEKIIAITFDDGPDPTYTPQILNLLRQYKAEATFFMIGFRVQRNPYLVKQVLKEGHEIGNH
TMNHLYASNSSDEKLENDILDGKKFFEKWVKEPLLFRPPGGYINDAVFKTAKEAGYQTVLWSWHQDPRDWANPGVQSIVN
HVLKNAKSGDIVLLHDGGNNRSQTVAALAKILPELKKQGYRFVTVSELLRYKH

Sequences:

>Translated_213_residues
MLLRKELEPTGYVTWEVPNNEKIIAITFDDGPDPTYTPQILNLLRQYKAEATFFMIGFRVQRNPYLVKQVLKEGHEIGNH
TMNHLYASNSSDEKLENDILDGKKFFEKWVKEPLLFRPPGGYINDAVFKTAKEAGYQTVLWSWHQDPRDWANPGVQSIVN
HVLKNAKSGDIVLLHDGGNNRSQTVAALAKILPELKKQGYRFVTVSELLRYKH
>Mature_213_residues
MLLRKELEPTGYVTWEVPNNEKIIAITFDDGPDPTYTPQILNLLRQYKAEATFFMIGFRVQRNPYLVKQVLKEGHEIGNH
TMNHLYASNSSDEKLENDILDGKKFFEKWVKEPLLFRPPGGYINDAVFKTAKEAGYQTVLWSWHQDPRDWANPGVQSIVN
HVLKNAKSGDIVLLHDGGNNRSQTVAALAKILPELKKQGYRFVTVSELLRYKH

Specific function: Is involved in generating a small heat-stable compound (Nod), an acylated oligomer of N-acetylglucosamine, that stimulates mitosis in various plant protoplasts [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323339, Length=182, Percent_Identity=32.4175824175824, Blast_Score=79, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323338, Length=132, Percent_Identity=35.6060606060606, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 24480; Mature: 24480

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLRKELEPTGYVTWEVPNNEKIIAITFDDGPDPTYTPQILNLLRQYKAEATFFMIGFRV
CCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEEEEEE
QRNPYLVKQVLKEGHEIGNHTMNHLYASNSSDEKLENDILDGKKFFEKWVKEPLLFRPPG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHCCEEECCCC
GYINDAVFKTAKEAGYQTVLWSWHQDPRDWANPGVQSIVNHVLKNAKSGDIVLLHDGGNN
CCHHHHHHHHHHHCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCCC
RSQTVAALAKILPELKKQGYRFVTVSELLRYKH
HHHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCC
>Mature Secondary Structure
MLLRKELEPTGYVTWEVPNNEKIIAITFDDGPDPTYTPQILNLLRQYKAEATFFMIGFRV
CCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEEEEEE
QRNPYLVKQVLKEGHEIGNHTMNHLYASNSSDEKLENDILDGKKFFEKWVKEPLLFRPPG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHCCEEECCCC
GYINDAVFKTAKEAGYQTVLWSWHQDPRDWANPGVQSIVNHVLKNAKSGDIVLLHDGGNN
CCHHHHHHHHHHHCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCCC
RSQTVAALAKILPELKKQGYRFVTVSELLRYKH
HHHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7984092; 9163424 [H]