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The map label for this gene is alaS [H]

Identifier: 52141918

GI number: 52141918

Start: 3459853

End: 3461088

Strand: Direct

Name: alaS [H]

Synonym: BCZK3326

Alternate gene names: 52141918

Gene position: 3459853-3461088 (Clockwise)

Preceding gene: 52141920

Following gene: 52141917

Centisome position: 65.27

GC content: 36.97

Gene sequence:

>1236_bases
ATGAAACTAGCGAACACGAAAGGGGAAAGAAAATTGGAGCAAAAATTATATTACACTGACGCTTATAAGCAAGACTTTAC
TACAAAAGTTATAAAGCAAGATTATGATAAAGACGGTAACTTATACGTTATTTTAAACGAAACGGTATTTTACCCAACAG
GTGGCGGGCAACCCCATGATACTGGAACTTTAAATGGCATTGCTGTACTTGGTGTTGAAGAAGTCGACGAAGAAATCCGC
CACTTCATTGCAGAACAATTACATACAGAAGAAGTAGAAGGTAAAATTAATTGGGAGCGCCGTTTTGATCATATGCAACA
ACATGCGGCTCAGCACATTTTATCTGCTGCTTTTTGGGATTACTTTAACATACCTACGATTGGATTCCATCTTGGTAAAG
AAACGGTAACAATTGATTTAGAAACAGAAAATTTACATGCAGAAACGGTTGAAAAAGCAGTACAAATTGCGAACAAAATT
GTTTTTGAAAATCATCCAATTCTTATTAAATGGATGAACTTAGAAGAAGCGAAAACATTACCACTTCGCAAAGAACCGAC
TATGACAGAAAATATACGCGTTGTTATTATTGAAAACTTTGATTATAACGGCTGTGGTGGAACGCACCCGAAACGTACAG
GTGAAGTAGGGCCTATTCAAGTACTAGGATGGGAACGTAATAAAGGCGGCATACGATTAACGTTTATCGCTGGTTGGCGC
ACACTTAAATTAATGGGACAACAGCAACAAATTATGAAGGATGTTTCTAAACAATTAAACAGTAGCGAAGCTGATATTCC
AGCAAAAGTAGCACAACTTCTTACTTCTCAAAAAGAAAATGAAAAAGCAATGCAAACAATGACTGAAAAATTATTATATA
CAGAGGCAAACGAACTACTTCAACAACCTACCAAAATACATGCTGGCATACTTATTTCTAAAGTGTTTACAAATCGTTCT
ATGCAAGAAATCGCAAAGCTATCCGCTATTATTACAGAACAACAAGAGCATGCCATTACATATTTCGTTATTGAAAACGA
GGACAAATTACAATGCATCCTCGCTTGTGGAAAAACAGTCACACTCGATATGAATGCTCTTTTAAAAGAGGCACTTCCTA
CAATTGAAGGAAAAGGTGGCGGAAATAAAAAGAGTGCTCGCGGCGGTGGGAAAGCGATGATGAGCGGGGATGAGTTTTTA
AATCAACTTATTTCTTCTTTACAGTCAGCAGTCTAA

Upstream 100 bases:

>100_bases
ATTCCACCCCCGCATTTTCTTCCTCTTATTATAGCAAGAAAATTCTAAAAATAGTTTCTTTCTTCTGAAATATTAGGAAA
CGAATTTCGATTGCTTTACA

Downstream 100 bases:

>100_bases
AACCTGTTTATGAAGGATAGCTAACGGCTATCCTTCCACTATATTTATAGGCCTATATTTCGTTTTAACCCTTGATAACT
CCCCGCATTTAGAAAACCTT

Product: alanine--tRNA ligase (alanyl-tRNA synthetase)

Products: AMP; diphosphate; L-alanyl-tRNA(Ala)

Alternate protein names: AlaX-L; Alanyl-tRNA deacylase AlaX-L [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MKLANTKGERKLEQKLYYTDAYKQDFTTKVIKQDYDKDGNLYVILNETVFYPTGGGQPHDTGTLNGIAVLGVEEVDEEIR
HFIAEQLHTEEVEGKINWERRFDHMQQHAAQHILSAAFWDYFNIPTIGFHLGKETVTIDLETENLHAETVEKAVQIANKI
VFENHPILIKWMNLEEAKTLPLRKEPTMTENIRVVIIENFDYNGCGGTHPKRTGEVGPIQVLGWERNKGGIRLTFIAGWR
TLKLMGQQQQIMKDVSKQLNSSEADIPAKVAQLLTSQKENEKAMQTMTEKLLYTEANELLQQPTKIHAGILISKVFTNRS
MQEIAKLSAIITEQQEHAITYFVIENEDKLQCILACGKTVTLDMNALLKEALPTIEGKGGGNKKSARGGGKAMMSGDEFL
NQLISSLQSAV

Sequences:

>Translated_411_residues
MKLANTKGERKLEQKLYYTDAYKQDFTTKVIKQDYDKDGNLYVILNETVFYPTGGGQPHDTGTLNGIAVLGVEEVDEEIR
HFIAEQLHTEEVEGKINWERRFDHMQQHAAQHILSAAFWDYFNIPTIGFHLGKETVTIDLETENLHAETVEKAVQIANKI
VFENHPILIKWMNLEEAKTLPLRKEPTMTENIRVVIIENFDYNGCGGTHPKRTGEVGPIQVLGWERNKGGIRLTFIAGWR
TLKLMGQQQQIMKDVSKQLNSSEADIPAKVAQLLTSQKENEKAMQTMTEKLLYTEANELLQQPTKIHAGILISKVFTNRS
MQEIAKLSAIITEQQEHAITYFVIENEDKLQCILACGKTVTLDMNALLKEALPTIEGKGGGNKKSARGGGKAMMSGDEFL
NQLISSLQSAV
>Mature_411_residues
MKLANTKGERKLEQKLYYTDAYKQDFTTKVIKQDYDKDGNLYVILNETVFYPTGGGQPHDTGTLNGIAVLGVEEVDEEIR
HFIAEQLHTEEVEGKINWERRFDHMQQHAAQHILSAAFWDYFNIPTIGFHLGKETVTIDLETENLHAETVEKAVQIANKI
VFENHPILIKWMNLEEAKTLPLRKEPTMTENIRVVIIENFDYNGCGGTHPKRTGEVGPIQVLGWERNKGGIRLTFIAGWR
TLKLMGQQQQIMKDVSKQLNSSEADIPAKVAQLLTSQKENEKAMQTMTEKLLYTEANELLQQPTKIHAGILISKVFTNRS
MQEIAKLSAIITEQQEHAITYFVIENEDKLQCILACGKTVTLDMNALLKEALPTIEGKGGGNKKSARGGGKAMMSGDEFL
NQLISSLQSAV

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-L subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=376, Percent_Identity=30.8510638297872, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI217416402, Length=376, Percent_Identity=30.8510638297872, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1789048, Length=365, Percent_Identity=23.8356164383562, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324288, Length=293, Percent_Identity=24.5733788395904, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI18543305, Length=396, Percent_Identity=26.7676767676768, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7

Molecular weight: Translated: 46286; Mature: 46286

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLANTKGERKLEQKLYYTDAYKQDFTTKVIKQDYDKDGNLYVILNETVFYPTGGGQPHD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECEEEEECCCCCCCC
TGTLNGIAVLGVEEVDEEIRHFIAEQLHTEEVEGKINWERRFDHMQQHAAQHILSAAFWD
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
YFNIPTIGFHLGKETVTIDLETENLHAETVEKAVQIANKIVFENHPILIKWMNLEEAKTL
HCCCCEEEEEECCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
PLRKEPTMTENIRVVIIENFDYNGCGGTHPKRTGEVGPIQVLGWERNKGGIRLTFIAGWR
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCH
TLKLMGQQQQIMKDVSKQLNSSEADIPAKVAQLLTSQKENEKAMQTMTEKLLYTEANELL
HHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
QQPTKIHAGILISKVFTNRSMQEIAKLSAIITEQQEHAITYFVIENEDKLQCILACGKTV
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEECCCEE
TLDMNALLKEALPTIEGKGGGNKKSARGGGKAMMSGDEFLNQLISSLQSAV
EEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLANTKGERKLEQKLYYTDAYKQDFTTKVIKQDYDKDGNLYVILNETVFYPTGGGQPHD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECEEEEECCCCCCCC
TGTLNGIAVLGVEEVDEEIRHFIAEQLHTEEVEGKINWERRFDHMQQHAAQHILSAAFWD
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
YFNIPTIGFHLGKETVTIDLETENLHAETVEKAVQIANKIVFENHPILIKWMNLEEAKTL
HCCCCEEEEEECCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
PLRKEPTMTENIRVVIIENFDYNGCGGTHPKRTGEVGPIQVLGWERNKGGIRLTFIAGWR
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCH
TLKLMGQQQQIMKDVSKQLNSSEADIPAKVAQLLTSQKENEKAMQTMTEKLLYTEANELL
HHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
QQPTKIHAGILISKVFTNRSMQEIAKLSAIITEQQEHAITYFVIENEDKLQCILACGKTV
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEECCCEE
TLDMNALLKEALPTIEGKGGGNKKSARGGGKAMMSGDEFLNQLISSLQSAV
EEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-alanine; tRNA(Ala)

Specific reaction: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]