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The map label for this gene is kinA [H]

Identifier: 52141898

GI number: 52141898

Start: 3475085

End: 3476617

Strand: Direct

Name: kinA [H]

Synonym: BCZK3344

Alternate gene names: 52141898

Gene position: 3475085-3476617 (Clockwise)

Preceding gene: 52141896

Following gene: 52141880

Centisome position: 65.56

GC content: 33.59

Gene sequence:

>1533_bases
ATGAGTCGTAAAAAATATTCATCAACTCAAAAAAAACGTCATCCGAACATTTCTAGACGTTTGCGAAACCATATACAAGA
TCGTTCATTAACAGAAATGTATGCATCCCTGTTCGAACACAATCCTGATAGTATTATTTCATTAAATTTAGAAGGAGTTA
TTCTACATATAAATCCTTCTGCCGAAAGAATATTAGGTTACACTTCTTTAGAATTAGAACAGAAAAATATTACCTCTATT
TTAGAAGCCCATATTTCTGAGCAAGTATTACAAAATATAAAGAATACTGAAGTGGATAATCAACAAGAGTATATTTTATC
TATTTGTCATAAAAATGGATTCTTATTAGACGTCGTGACAAAACTAGTTCCTATTTTTGTCCAAAATCGTCTTACAGGTG
TATATACCATTATGAAGCCGCTTGAAAAATCAGAAAGAATTGAGAATATGCTAAAAGAAAGTGAGAAGAGATTACGTACA
TTAGTGAACTCTATGCCTGCGTTTGTTATATTTAAAGATCATGAAGGACGCTGGCTTGAGGCAAATGACTACGCACTTTC
TTGTTTCAATTTCCATCATGTACCTTACCGTGGGAAAAAAGATAGTGAACTCATTCAATATAATGAAGCGTACCGAGAAG
CTTTTTTACATTGCGAAGAAGTCGATGAACTAGCATGGCAACAAAGACAAATTCTTCATGGTGAAGAATTTATTATTCAT
AGAGATGATTTCGACCTAATCTTAAGTATGTCGAAAGTTCCTCTCTATCATCCTGATGGCTCCCGTAAAGGTCTTATCGT
AATGGGAAGAGACGTTACTGAGTTAAAAGAAACAGAAAAATTATTACGAAAATCAGAAAAACTCGCAGTAGTCGGACAAC
TGACAGCTGGAATTGCCCATGAAATTCGAAATCCACTCACTTCTCTAAAGGGTTTTCTAACATTGCTACTCCCTGAAATA
AATGAGGAGCATAAATGGTATATTGATGTTATGTTAAGTGAAATTTCACAGATGGAATCTATCACGAGTCAATTTATGGC
GATGTCTAAACCACAAGTGTTAGCAATCCAAACTTGTAACATACAAACTTTAATTGAAGAGGTAGTTACATTTATTTTAC
CGACTGCTATTATGCACAGTGTTCATATCATTATGGATCATTTTGATTACGTCTATGACATTCAATGTGACGGTAACCAA
TTAAAACAAGTTTTTATAAATATATTAAAAAATGCGATAGAAGCGATGCCAAATGGCGGAAATATTTTCATCCAAACAAA
ACCATTAGAGGATAATTTCATCTTAATACGAATTATCGATGAAGGTTGCGGCATTCCAGAAGATCGTATCTCCCGTCTAG
GCGAACCGTTTTATAGTTTAAAAGAAAAAGGCACAGGACTAGGTTTAATGATGTGCTACAAAATAATTGAAGAACATCAT
GGTAAATTACACATTTCAAGTGAATTAAATAAAGGTACAATCGTTGATATTCAGCTACCACTTTCTTCATCTCATCTTGC
AATCCAGTCTTAA

Upstream 100 bases:

>100_bases
ATTATATAACTTCCATTTTTTATAGAAATACACGTTACATCTATATTATAGTTATGAATAGACAAATCTTTTTAGATGCT
ATTTGTACGGAGGAACTATT

Downstream 100 bases:

>100_bases
AAAATAAAAGAAGCATCTGCTAATGATTAGCAGATGCTTCTTTTATTTTTTGTACTTATAGAACCGTTTTCTCATACCAC
TCTTTTGCTTTTTCTACTTC

Product: sporulation kinase A

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MSRKKYSSTQKKRHPNISRRLRNHIQDRSLTEMYASLFEHNPDSIISLNLEGVILHINPSAERILGYTSLELEQKNITSI
LEAHISEQVLQNIKNTEVDNQQEYILSICHKNGFLLDVVTKLVPIFVQNRLTGVYTIMKPLEKSERIENMLKESEKRLRT
LVNSMPAFVIFKDHEGRWLEANDYALSCFNFHHVPYRGKKDSELIQYNEAYREAFLHCEEVDELAWQQRQILHGEEFIIH
RDDFDLILSMSKVPLYHPDGSRKGLIVMGRDVTELKETEKLLRKSEKLAVVGQLTAGIAHEIRNPLTSLKGFLTLLLPEI
NEEHKWYIDVMLSEISQMESITSQFMAMSKPQVLAIQTCNIQTLIEEVVTFILPTAIMHSVHIIMDHFDYVYDIQCDGNQ
LKQVFINILKNAIEAMPNGGNIFIQTKPLEDNFILIRIIDEGCGIPEDRISRLGEPFYSLKEKGTGLGLMMCYKIIEEHH
GKLHISSELNKGTIVDIQLPLSSSHLAIQS

Sequences:

>Translated_510_residues
MSRKKYSSTQKKRHPNISRRLRNHIQDRSLTEMYASLFEHNPDSIISLNLEGVILHINPSAERILGYTSLELEQKNITSI
LEAHISEQVLQNIKNTEVDNQQEYILSICHKNGFLLDVVTKLVPIFVQNRLTGVYTIMKPLEKSERIENMLKESEKRLRT
LVNSMPAFVIFKDHEGRWLEANDYALSCFNFHHVPYRGKKDSELIQYNEAYREAFLHCEEVDELAWQQRQILHGEEFIIH
RDDFDLILSMSKVPLYHPDGSRKGLIVMGRDVTELKETEKLLRKSEKLAVVGQLTAGIAHEIRNPLTSLKGFLTLLLPEI
NEEHKWYIDVMLSEISQMESITSQFMAMSKPQVLAIQTCNIQTLIEEVVTFILPTAIMHSVHIIMDHFDYVYDIQCDGNQ
LKQVFINILKNAIEAMPNGGNIFIQTKPLEDNFILIRIIDEGCGIPEDRISRLGEPFYSLKEKGTGLGLMMCYKIIEEHH
GKLHISSELNKGTIVDIQLPLSSSHLAIQS
>Mature_509_residues
SRKKYSSTQKKRHPNISRRLRNHIQDRSLTEMYASLFEHNPDSIISLNLEGVILHINPSAERILGYTSLELEQKNITSIL
EAHISEQVLQNIKNTEVDNQQEYILSICHKNGFLLDVVTKLVPIFVQNRLTGVYTIMKPLEKSERIENMLKESEKRLRTL
VNSMPAFVIFKDHEGRWLEANDYALSCFNFHHVPYRGKKDSELIQYNEAYREAFLHCEEVDELAWQQRQILHGEEFIIHR
DDFDLILSMSKVPLYHPDGSRKGLIVMGRDVTELKETEKLLRKSEKLAVVGQLTAGIAHEIRNPLTSLKGFLTLLLPEIN
EEHKWYIDVMLSEISQMESITSQFMAMSKPQVLAIQTCNIQTLIEEVVTFILPTAIMHSVHIIMDHFDYVYDIQCDGNQL
KQVFINILKNAIEAMPNGGNIFIQTKPLEDNFILIRIIDEGCGIPEDRISRLGEPFYSLKEKGTGLGLMMCYKIIEEHHG
KLHISSELNKGTIVDIQLPLSSSHLAIQS

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=397, Percent_Identity=27.455919395466, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1790436, Length=224, Percent_Identity=32.1428571428571, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI48994928, Length=372, Percent_Identity=21.7741935483871, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790300, Length=206, Percent_Identity=30.0970873786408, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1786600, Length=363, Percent_Identity=23.9669421487603, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788713, Length=417, Percent_Identity=22.5419664268585, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789149, Length=226, Percent_Identity=25.6637168141593, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI145693157, Length=222, Percent_Identity=27.027027027027, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790551, Length=205, Percent_Identity=26.8292682926829, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788393, Length=244, Percent_Identity=22.1311475409836, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790861, Length=218, Percent_Identity=21.5596330275229, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58715; Mature: 58584

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKKYSSTQKKRHPNISRRLRNHIQDRSLTEMYASLFEHNPDSIISLNLEGVILHINPS
CCCCCCCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCC
AERILGYTSLELEQKNITSILEAHISEQVLQNIKNTEVDNQQEYILSICHKNGFLLDVVT
HHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHH
KLVPIFVQNRLTGVYTIMKPLEKSERIENMLKESEKRLRTLVNSMPAFVIFKDHEGRWLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEE
ANDYALSCFNFHHVPYRGKKDSELIQYNEAYREAFLHCEEVDELAWQQRQILHGEEFIIH
CCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
RDDFDLILSMSKVPLYHPDGSRKGLIVMGRDVTELKETEKLLRKSEKLAVVGQLTAGIAH
CCCHHHHEECCCCEEECCCCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
EIRNPLTSLKGFLTLLLPEINEEHKWYIDVMLSEISQMESITSQFMAMSKPQVLAIQTCN
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
IQTLIEEVVTFILPTAIMHSVHIIMDHFDYVYDIQCDGNQLKQVFINILKNAIEAMPNGG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCC
NIFIQTKPLEDNFILIRIIDEGCGIPEDRISRLGEPFYSLKEKGTGLGLMMCYKIIEEHH
EEEEEECCCCCCEEEEEEECCCCCCCHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHCC
GKLHISSELNKGTIVDIQLPLSSSHLAIQS
CEEEEECCCCCCEEEEEEECCCCCCCEECC
>Mature Secondary Structure 
SRKKYSSTQKKRHPNISRRLRNHIQDRSLTEMYASLFEHNPDSIISLNLEGVILHINPS
CCCCCCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCC
AERILGYTSLELEQKNITSILEAHISEQVLQNIKNTEVDNQQEYILSICHKNGFLLDVVT
HHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHH
KLVPIFVQNRLTGVYTIMKPLEKSERIENMLKESEKRLRTLVNSMPAFVIFKDHEGRWLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEE
ANDYALSCFNFHHVPYRGKKDSELIQYNEAYREAFLHCEEVDELAWQQRQILHGEEFIIH
CCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
RDDFDLILSMSKVPLYHPDGSRKGLIVMGRDVTELKETEKLLRKSEKLAVVGQLTAGIAH
CCCHHHHEECCCCEEECCCCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
EIRNPLTSLKGFLTLLLPEINEEHKWYIDVMLSEISQMESITSQFMAMSKPQVLAIQTCN
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
IQTLIEEVVTFILPTAIMHSVHIIMDHFDYVYDIQCDGNQLKQVFINILKNAIEAMPNGG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCC
NIFIQTKPLEDNFILIRIIDEGCGIPEDRISRLGEPFYSLKEKGTGLGLMMCYKIIEEHH
EEEEEECCCCCCEEEEEEECCCCCCCHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHCC
GKLHISSELNKGTIVDIQLPLSSSHLAIQS
CEEEEECCCCCCEEEEEEECCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]