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The map label for this gene is amaA [H]

Identifier: 52141875

GI number: 52141875

Start: 3506809

End: 3507840

Strand: Direct

Name: amaA [H]

Synonym: BCZK3370

Alternate gene names: 52141875

Gene position: 3506809-3507840 (Clockwise)

Preceding gene: 52141878

Following gene: 52141870

Centisome position: 66.15

GC content: 40.12

Gene sequence:

>1032_bases
TTGGTTGGAAGAAAAAACATTACGATTATACATTCTAATTTAGAAACAGGCGTTATTGCTGAAATTTCTGGAAATAGTAA
CGGACCACTTATAGCAATCCGTGCTGATATTGATGCCCTGCCTATTCAAGAGGAAACAAATTTGCCGTACGCTTCAAAAA
TTCACGGCAGGATGCATGCTTGCGGACATGATTTTCATACAGCTGCTATTATAGGGGCTGCTTATTTGCTAAAAGAAAAA
GAATCTTCTCTTAGCGGAACTGTACGCTTTATATTCCAACCAGCTGAAGAAAGTAGTAATGGTGCTTGCAAAGTAATTGA
AGCCGGGCATTTACATGGCGTGCAAGCTATTTTCGGTATGCATAATAAACCTGATTTACCAGTCGGTACAATCGGTATTA
AAGATGGTCCACTGATGGCTGGAGTTGATCGATTTGAAATTGAAATTCACGGAGTTGGTACACATGCAGCTGTACCAGAT
GCTGGGGTAGACCCTATTGTCGCGTCTTCTCAAATTGTGATGGCACTACAAACAATTGTAAGCCGAAATATAAGTTCTTC
ACATAACGCCGTCGTTAGTGTCACAAACATCCACTCAGGAAATACGTGGAACGTTATTCCTGAAAAAGCAACGCTGGAAG
GAACTGTACGTACATTTCAAAATGAAACACGTGAAAAGATTCCTGCGCTAATGAAACGGATTATTCAAGGGGTTTCTGAT
GCATTAGGTGTAAAAACAGAATTTCGTTTTTATGCAGGTCCTCCTGCTGTTCATAACGATACATCTCTAACAAACTTATC
GACTCAAGTTGCAGAAACTATGAATTTAAATATCGTCTCTCCTACCCCGTCAATGGCCGGAGAAGATTTTTCTTTTTATC
AACAAGAAATACCTGGTTCCTTCGTCTTTATGGGAACAAGTGGTACACACGAATGGCATCATCCCGCTTTTACAGTAGAT
GAGCGAGCATTACCTATAAGTGCAGAATACTTCGCTTTGCTAGCTGAAAGGGCACTTAAACAATTTGCATAA

Upstream 100 bases:

>100_bases
TAGAGCAATTAACTGAGACCCTAATTTCTATTCGTCGAAATTTACATGAGCATCCAGAACTATCATACGAAGAATTTGAA
ACGACGAAAGCTATAAAGAA

Downstream 100 bases:

>100_bases
AAAAGGAGCCGTTAAGACATATTTAACGGCTCCCTTTTTAAGTTAAATCACGTTTCAAAAATACTGTATAAGAAACGCTT
AAAAATACAAATAAATACAG

Product: N-acyl-L-amino acid amidohydrolase (aminoacylase) (hippuricase)

Products: carboxylate; L-amino acid

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MVGRKNITIIHSNLETGVIAEISGNSNGPLIAIRADIDALPIQEETNLPYASKIHGRMHACGHDFHTAAIIGAAYLLKEK
ESSLSGTVRFIFQPAEESSNGACKVIEAGHLHGVQAIFGMHNKPDLPVGTIGIKDGPLMAGVDRFEIEIHGVGTHAAVPD
AGVDPIVASSQIVMALQTIVSRNISSSHNAVVSVTNIHSGNTWNVIPEKATLEGTVRTFQNETREKIPALMKRIIQGVSD
ALGVKTEFRFYAGPPAVHNDTSLTNLSTQVAETMNLNIVSPTPSMAGEDFSFYQQEIPGSFVFMGTSGTHEWHHPAFTVD
ERALPISAEYFALLAERALKQFA

Sequences:

>Translated_343_residues
MVGRKNITIIHSNLETGVIAEISGNSNGPLIAIRADIDALPIQEETNLPYASKIHGRMHACGHDFHTAAIIGAAYLLKEK
ESSLSGTVRFIFQPAEESSNGACKVIEAGHLHGVQAIFGMHNKPDLPVGTIGIKDGPLMAGVDRFEIEIHGVGTHAAVPD
AGVDPIVASSQIVMALQTIVSRNISSSHNAVVSVTNIHSGNTWNVIPEKATLEGTVRTFQNETREKIPALMKRIIQGVSD
ALGVKTEFRFYAGPPAVHNDTSLTNLSTQVAETMNLNIVSPTPSMAGEDFSFYQQEIPGSFVFMGTSGTHEWHHPAFTVD
ERALPISAEYFALLAERALKQFA
>Mature_343_residues
MVGRKNITIIHSNLETGVIAEISGNSNGPLIAIRADIDALPIQEETNLPYASKIHGRMHACGHDFHTAAIIGAAYLLKEK
ESSLSGTVRFIFQPAEESSNGACKVIEAGHLHGVQAIFGMHNKPDLPVGTIGIKDGPLMAGVDRFEIEIHGVGTHAAVPD
AGVDPIVASSQIVMALQTIVSRNISSSHNAVVSVTNIHSGNTWNVIPEKATLEGTVRTFQNETREKIPALMKRIIQGVSD
ALGVKTEFRFYAGPPAVHNDTSLTNLSTQVAETMNLNIVSPTPSMAGEDFSFYQQEIPGSFVFMGTSGTHEWHHPAFTVD
ERALPISAEYFALLAERALKQFA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=337, Percent_Identity=31.7507418397626, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: 3.5.1.14

Molecular weight: Translated: 36893; Mature: 36893

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGRKNITIIHSNLETGVIAEISGNSNGPLIAIRADIDALPIQEETNLPYASKIHGRMHA
CCCCCEEEEEECCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHCCHHHH
CGHDFHTAAIIGAAYLLKEKESSLSGTVRFIFQPAEESSNGACKVIEAGHLHGVQAIFGM
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEECCCCHHHHHHHCC
HNKPDLPVGTIGIKDGPLMAGVDRFEIEIHGVGTHAAVPDAGVDPIVASSQIVMALQTIV
CCCCCCCEEEEECCCCCEEECCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
SRNISSSHNAVVSVTNIHSGNTWNVIPEKATLEGTVRTFQNETREKIPALMKRIIQGVSD
HCCCCCCCCEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGVKTEFRFYAGPPAVHNDTSLTNLSTQVAETMNLNIVSPTPSMAGEDFSFYQQEIPGS
HHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHCCCC
FVFMGTSGTHEWHHPAFTVDERALPISAEYFALLAERALKQFA
EEEEECCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVGRKNITIIHSNLETGVIAEISGNSNGPLIAIRADIDALPIQEETNLPYASKIHGRMHA
CCCCCEEEEEECCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHCCHHHH
CGHDFHTAAIIGAAYLLKEKESSLSGTVRFIFQPAEESSNGACKVIEAGHLHGVQAIFGM
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEECCCCHHHHHHHCC
HNKPDLPVGTIGIKDGPLMAGVDRFEIEIHGVGTHAAVPDAGVDPIVASSQIVMALQTIV
CCCCCCCEEEEECCCCCEEECCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
SRNISSSHNAVVSVTNIHSGNTWNVIPEKATLEGTVRTFQNETREKIPALMKRIIQGVSD
HCCCCCCCCEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGVKTEFRFYAGPPAVHNDTSLTNLSTQVAETMNLNIVSPTPSMAGEDFSFYQQEIPGS
HHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHCCCC
FVFMGTSGTHEWHHPAFTVDERALPISAEYFALLAERALKQFA
EEEEECCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-acyl-L-amino acid; H2O

Specific reaction: An N-acyl-L-amino acid + H2O = a carboxylate + an L-amino acid

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]