Click here to switch to the map view.

The map label for this gene is ply511 [H]

Identifier: 52141838

GI number: 52141838

Start: 3542072

End: 3543127

Strand: Reverse

Name: ply511 [H]

Synonym: BCZK3407

Alternate gene names: 52141838

Gene position: 3543127-3542072 (Counterclockwise)

Preceding gene: 52141837

Following gene: 52141841

Centisome position: 66.84

GC content: 37.03

Gene sequence:

>1056_bases
ATGAAAAAGAAGTGGAAACATATTTCATCTATAGTTGTCGCTATGATTTTAGTATTATCAATTGGAACAAACGTATTTGC
TGATAGGGTTTTGATTATTCCTGATTTACCAAAACAACCATACCGTAATGGTGTTGGTGCTTATGAAGGTGTTGTAGCAC
ATTCTACAGCTACCCCAGAAGCTCCAGCTATTAATATTCAAAAATATGAGTCTCGTACATGGCGCTCAGCTTTTGTTCAC
TACGCAGTTGATTGGGATGAAAAGATTCAAATTGCATCTACTAAATATCGTGCATGGGGTGCAGGTCCAGCAGCGAATGC
TAGATTTGTTCATATTGAATTGTGTGAAACAAGTGATCCGATGAAATTTAAACGTTCTTACGAACGATATGTAGAGTTGA
TTGGGGAAATCTTACGGGAACGAAATATTCATCCTTCTAAAGGATTATGGACACATAAGGATATTACTTATAAATTAGGT
GGTACAGACCATGAAGACCCACTTGATTATCTTCGTAGTCATGGTGTATCAGAGGCTGAATTCCGAGCAGATGTGTTAAA
GGCTTATAACGGGAACTCTGTTACGGTGGATGCAAAACCACAGAAACCAAATGAAATGCCTGGTACAGTAGAGGTGAATG
GTGTTGCGTATATTCAGGGGGACAATGTAAATCTTCGTTCTGGGCCATCAACAGATAATAGTGTTATTCGTAAACTACAA
AAAGGAGAGGCTTATAAAGTATGGGGTAAATTAGGAAACTGGTTAAATCTTGGTGGTAACCAATGGATTTATTATGACTC
CTCATACATCCGTTATAATGGTACGAATGCTTCTACTGTAGCTGGTAAAAAGATTATTTCTAAAGTGGACAATTTACGTT
TCTATGAATCTCCATCTTGGCGGGATAAAGAAGTTGCTGGAATAGTAGATAAAGGGATGGGATTCATTATAAGTTCAAAA
GTCACAGTGAATGGTTCACCGCAATACAAAGTACAGAATAGTAAAGGGAAAACATATTATATAACTGCAAGTGAATCATA
TGTATATGTGAAGTAA

Upstream 100 bases:

>100_bases
AAGATATCAAAACAAATATATTTCAAGGAATTATTATTGGACTGTCAGCGAGTGGATTATTTGACCACACAAAAATTATA
AAAAAGGATGCCGATGTAAA

Downstream 100 bases:

>100_bases
TTGAAAAGGGGAATTAGTAAAGTAATTGTGATTATATAAAATGAATTTTAAAAGTTTATTTTGTACTCAAATCAACTGCA
TATATATGGTTGATTTGAGT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: AtlE; N-acetylmuramoyl-L-alanine amidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MKKKWKHISSIVVAMILVLSIGTNVFADRVLIIPDLPKQPYRNGVGAYEGVVAHSTATPEAPAINIQKYESRTWRSAFVH
YAVDWDEKIQIASTKYRAWGAGPAANARFVHIELCETSDPMKFKRSYERYVELIGEILRERNIHPSKGLWTHKDITYKLG
GTDHEDPLDYLRSHGVSEAEFRADVLKAYNGNSVTVDAKPQKPNEMPGTVEVNGVAYIQGDNVNLRSGPSTDNSVIRKLQ
KGEAYKVWGKLGNWLNLGGNQWIYYDSSYIRYNGTNASTVAGKKIISKVDNLRFYESPSWRDKEVAGIVDKGMGFIISSK
VTVNGSPQYKVQNSKGKTYYITASESYVYVK

Sequences:

>Translated_351_residues
MKKKWKHISSIVVAMILVLSIGTNVFADRVLIIPDLPKQPYRNGVGAYEGVVAHSTATPEAPAINIQKYESRTWRSAFVH
YAVDWDEKIQIASTKYRAWGAGPAANARFVHIELCETSDPMKFKRSYERYVELIGEILRERNIHPSKGLWTHKDITYKLG
GTDHEDPLDYLRSHGVSEAEFRADVLKAYNGNSVTVDAKPQKPNEMPGTVEVNGVAYIQGDNVNLRSGPSTDNSVIRKLQ
KGEAYKVWGKLGNWLNLGGNQWIYYDSSYIRYNGTNASTVAGKKIISKVDNLRFYESPSWRDKEVAGIVDKGMGFIISSK
VTVNGSPQYKVQNSKGKTYYITASESYVYVK
>Mature_351_residues
MKKKWKHISSIVVAMILVLSIGTNVFADRVLIIPDLPKQPYRNGVGAYEGVVAHSTATPEAPAINIQKYESRTWRSAFVH
YAVDWDEKIQIASTKYRAWGAGPAANARFVHIELCETSDPMKFKRSYERYVELIGEILRERNIHPSKGLWTHKDITYKLG
GTDHEDPLDYLRSHGVSEAEFRADVLKAYNGNSVTVDAKPQKPNEMPGTVEVNGVAYIQGDNVNLRSGPSTDNSVIRKLQ
KGEAYKVWGKLGNWLNLGGNQWIYYDSSYIRYNGTNASTVAGKKIISKVDNLRFYESPSWRDKEVAGIVDKGMGFIISSK
VTVNGSPQYKVQNSKGKTYYITASESYVYVK

Specific function: Endohydrolysis of the di-N-acetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -[(Man)5(GlcNAc)2]-Asn structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is relea

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted. Note=Secreted, and then anchored on the cell surface at the peripheral cell wall above the completed septum (septal region), for the next cell division cycle [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01510 Amidase_2; PF01832 Glucosaminidase [H]

EC number: =3.5.1.28; =3.2.1.96 [H]

Molecular weight: Translated: 39443; Mature: 39443

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKWKHISSIVVAMILVLSIGTNVFADRVLIIPDLPKQPYRNGVGAYEGVVAHSTATPE
CCCHHHHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCCCHHHCCCCCCCCEEEECCCCCC
APAINIQKYESRTWRSAFVHYAVDWDEKIQIASTKYRAWGAGPAANARFVHIELCETSDP
CCEEEEEHHHCHHHHEEEEEEEECCCCEEEEEEEEEEECCCCCCCCEEEEEEEEECCCCC
MKFKRSYERYVELIGEILRERNIHPSKGLWTHKDITYKLGGTDHEDPLDYLRSHGVSEAE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEECCCCCCCHHHHHHHCCCCHHH
FRADVLKAYNGNSVTVDAKPQKPNEMPGTVEVNGVAYIQGDNVNLRSGPSTDNSVIRKLQ
HHHHHHHHCCCCEEEEECCCCCCCCCCCEEEECEEEEEECCCEEECCCCCCHHHHHHHHH
KGEAYKVWGKLGNWLNLGGNQWIYYDSSYIRYNGTNASTVAGKKIISKVDNLRFYESPSW
CCCEEEEHHHCCCEEECCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHHCCEEECCCCC
RDKEVAGIVDKGMGFIISSKVTVNGSPQYKVQNSKGKTYYITASESYVYVK
CCCHHHHHHHCCCCEEEEEEEEECCCCCEEEECCCCCEEEEEECCCEEEEC
>Mature Secondary Structure
MKKKWKHISSIVVAMILVLSIGTNVFADRVLIIPDLPKQPYRNGVGAYEGVVAHSTATPE
CCCHHHHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCCCHHHCCCCCCCCEEEECCCCCC
APAINIQKYESRTWRSAFVHYAVDWDEKIQIASTKYRAWGAGPAANARFVHIELCETSDP
CCEEEEEHHHCHHHHEEEEEEEECCCCEEEEEEEEEEECCCCCCCCEEEEEEEEECCCCC
MKFKRSYERYVELIGEILRERNIHPSKGLWTHKDITYKLGGTDHEDPLDYLRSHGVSEAE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEECCCCCCCHHHHHHHCCCCHHH
FRADVLKAYNGNSVTVDAKPQKPNEMPGTVEVNGVAYIQGDNVNLRSGPSTDNSVIRKLQ
HHHHHHHHCCCCEEEEECCCCCCCCCCCEEEECEEEEEECCCEEECCCCCCHHHHHHHHH
KGEAYKVWGKLGNWLNLGGNQWIYYDSSYIRYNGTNASTVAGKKIISKVDNLRFYESPSW
CCCEEEEHHHCCCEEECCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHHCCEEECCCCC
RDKEVAGIVDKGMGFIISSKVTVNGSPQYKVQNSKGKTYYITASESYVYVK
CCCHHHHHHHCCCCEEEEEEEEECCCCCEEEECCCCCEEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9220008 [H]