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The map label for this gene is murE [H]

Identifier: 52141574

GI number: 52141574

Start: 3813410

End: 3814885

Strand: Reverse

Name: murE [H]

Synonym: BCZK3673

Alternate gene names: 52141574

Gene position: 3814885-3813410 (Counterclockwise)

Preceding gene: 52141573

Following gene: 52141575

Centisome position: 71.97

GC content: 37.13

Gene sequence:

>1476_bases
ATGAAGTTGCATACACTTGTATCATGTTTGCATGATTTTCCAGTTGTTCCAAAAGAAAATCCAGAAATCACATCTATAGA
AGCTGATTCACGTAAAGTGAAAGAGGGAAGCTTATTTGTATGTATGAAAGGGTATACGGTTGATAGCCATGATTTTGCTA
AGCAAGCAGCGGCACAAGGAGCGGCTGCTATTGTTGCGGAAAGACCAATTGATGTTGACGTTCCAGTTGTACTTGTGAAA
AACACTTTTCGTTCGTTAGCGGTTTTAGCTGATTATTTTTATGGTCAACCAACACACAAGTTACATTTAATCGGTATTAC
AGGTACAAATGGAAAGACAACAACATCGCATATTATGGATGAGATTATGCGCGCACATGGTCATAAAACGGGGCTAATTG
GAACGATTAATATGAAAATCGGTGATGAAACATTTGAGGTGAAAAATACAACGCCAGATGCACTAACACTTCAACAGACG
TTTGCAAAAATGGTTGAACACGGTGTGGATAGCACAGTAATGGAAGTTTCATCACATGCTTTAGATCTTGGTCGTGTACA
CGGATGTGATTACGATGTAGCAGTGTTTACAAATTTAACACAAGATCATTTAGACTACCATAAAACGATGGAAGAATATA
AACATGCAAAAGGGTTGCTATTTGCACAACTTGGCAATAGTTATAATCATAATCGTGAAAAATACGCGGTACTGAATAAC
GATGATGCTGTAACAGCGGAATATATGAGAAGTACTGCAGCAACTGTTGTAACGTATGGAATTGATACAACAAGTGATGT
TATGGCCAAAGATATCGTTATGACAAGTGGTGGAACTACATTTACGCTTGTAACACCGTATGAAAGTGTAAATGTTACGA
TGAAATTAATTGGGAAATTTAATGTATATAACGTACTAGCTGCAACAGCGGCAGGACTTGTTTCTGGTGTAAGTTTGGAA
ACAATCATTGATGTAATAAAGGAACTAGCTGGAGTTCCTGGACGTTTCGAAGTAGTTGATGGTGGACAAAATTATACAGT
TATTGTTGATTATGCACATACACCAGATAGCTTAGAAAATGTATTGAAAACAGCAAAACAGTTTGCAAAAGGTGACGTAT
ATTGCATCGTCGGTTGCGGCGGTGATAGAGACCGGACAAAGAGACCAATTATGGCAAGTGTTGCAACAAAATATGCAACT
CATGCAATTTATACATCAGATAATCCAAGAAGTGAAGATCCAGCGGCTATTTTAGATGATATGGTACATGGTGTAAGTGG
GAATAATTATGAAGTGATTATTGATAGAAAAGAAGCAATACACCATGCGATTACTAAAGCGAAAGCAGATGATATCATTA
TTATTGCTGGTAAGGGTCATGAAACATATCAAATTATTGGTAAAGATGTTCATCATTTTGACGATCGTGAAGTCGCTAAA
GAAGCAATTACAGAGCGTTTAAACAACGAAGAGTAA

Upstream 100 bases:

>100_bases
AGACACAGTAGCACTTACCTCCTATTTTAAATAATTAGGAGGTAGCGTGTTGCTTATTAATAAAAGAGATAAATACACAA
TGTAGAGAGGGTTTAGTGAA

Downstream 100 bases:

>100_bases
CAACGTGAGAGGAGGACTACATGTGCTTGAGCAAGGTTTATTAGTAACGGCTGGGGTAGCATTCTTAATTTCTGTTGCCC
TTTCGCCATTGTTTATTCCA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVKEGSLFVCMKGYTVDSHDFAKQAAAQGAAAIVAERPIDVDVPVVLVK
NTFRSLAVLADYFYGQPTHKLHLIGITGTNGKTTTSHIMDEIMRAHGHKTGLIGTINMKIGDETFEVKNTTPDALTLQQT
FAKMVEHGVDSTVMEVSSHALDLGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYNHNREKYAVLNN
DDAVTAEYMRSTAATVVTYGIDTTSDVMAKDIVMTSGGTTFTLVTPYESVNVTMKLIGKFNVYNVLAATAAGLVSGVSLE
TIIDVIKELAGVPGRFEVVDGGQNYTVIVDYAHTPDSLENVLKTAKQFAKGDVYCIVGCGGDRDRTKRPIMASVATKYAT
HAIYTSDNPRSEDPAAILDDMVHGVSGNNYEVIIDRKEAIHHAITKAKADDIIIIAGKGHETYQIIGKDVHHFDDREVAK
EAITERLNNEE

Sequences:

>Translated_491_residues
MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVKEGSLFVCMKGYTVDSHDFAKQAAAQGAAAIVAERPIDVDVPVVLVK
NTFRSLAVLADYFYGQPTHKLHLIGITGTNGKTTTSHIMDEIMRAHGHKTGLIGTINMKIGDETFEVKNTTPDALTLQQT
FAKMVEHGVDSTVMEVSSHALDLGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYNHNREKYAVLNN
DDAVTAEYMRSTAATVVTYGIDTTSDVMAKDIVMTSGGTTFTLVTPYESVNVTMKLIGKFNVYNVLAATAAGLVSGVSLE
TIIDVIKELAGVPGRFEVVDGGQNYTVIVDYAHTPDSLENVLKTAKQFAKGDVYCIVGCGGDRDRTKRPIMASVATKYAT
HAIYTSDNPRSEDPAAILDDMVHGVSGNNYEVIIDRKEAIHHAITKAKADDIIIIAGKGHETYQIIGKDVHHFDDREVAK
EAITERLNNEE
>Mature_491_residues
MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVKEGSLFVCMKGYTVDSHDFAKQAAAQGAAAIVAERPIDVDVPVVLVK
NTFRSLAVLADYFYGQPTHKLHLIGITGTNGKTTTSHIMDEIMRAHGHKTGLIGTINMKIGDETFEVKNTTPDALTLQQT
FAKMVEHGVDSTVMEVSSHALDLGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYNHNREKYAVLNN
DDAVTAEYMRSTAATVVTYGIDTTSDVMAKDIVMTSGGTTFTLVTPYESVNVTMKLIGKFNVYNVLAATAAGLVSGVSLE
TIIDVIKELAGVPGRFEVVDGGQNYTVIVDYAHTPDSLENVLKTAKQFAKGDVYCIVGCGGDRDRTKRPIMASVATKYAT
HAIYTSDNPRSEDPAAILDDMVHGVSGNNYEVIIDRKEAIHHAITKAKADDIIIIAGKGHETYQIIGKDVHHFDDREVAK
EAITERLNNEE

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=480, Percent_Identity=35.625, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI1786274, Length=339, Percent_Identity=28.9085545722714, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53706; Mature: 53706

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVKEGSLFVCMKGYTVDSHDFAKQAAAQG
CCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCEECCHHHHHHHHHCC
AAAIVAERPIDVDVPVVLVKNTFRSLAVLADYFYGQPTHKLHLIGITGTNGKTTTSHIMD
CEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHH
EIMRAHGHKTGLIGTINMKIGDETFEVKNTTPDALTLQQTFAKMVEHGVDSTVMEVSSHA
HHHHHCCCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
LDLGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYNHNREKYAVLNN
HHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECC
DDAVTAEYMRSTAATVVTYGIDTTSDVMAKDIVMTSGGTTFTLVTPYESVNVTMKLIGKF
CCCHHHHHHHHHHHEEEEECCCCCHHHHHHHEEEECCCCEEEEECCCCCCCEEEEEEECC
NVYNVLAATAAGLVSGVSLETIIDVIKELAGVPGRFEVVDGGQNYTVIVDYAHTPDSLEN
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCHHHHH
VLKTAKQFAKGDVYCIVGCGGDRDRTKRPIMASVATKYATHAIYTSDNPRSEDPAAILDD
HHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCHHHHHHH
MVHGVSGNNYEVIIDRKEAIHHAITKAKADDIIIIAGKGHETYQIIGKDVHHFDDREVAK
HHHCCCCCCEEEEEEHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCHHHCCCHHHHHH
EAITERLNNEE
HHHHHHHCCCC
>Mature Secondary Structure
MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVKEGSLFVCMKGYTVDSHDFAKQAAAQG
CCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCEECCHHHHHHHHHCC
AAAIVAERPIDVDVPVVLVKNTFRSLAVLADYFYGQPTHKLHLIGITGTNGKTTTSHIMD
CEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHH
EIMRAHGHKTGLIGTINMKIGDETFEVKNTTPDALTLQQTFAKMVEHGVDSTVMEVSSHA
HHHHHCCCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
LDLGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYNHNREKYAVLNN
HHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECC
DDAVTAEYMRSTAATVVTYGIDTTSDVMAKDIVMTSGGTTFTLVTPYESVNVTMKLIGKF
CCCHHHHHHHHHHHEEEEECCCCCHHHHHHHEEEECCCCEEEEECCCCCCCEEEEEEECC
NVYNVLAATAAGLVSGVSLETIIDVIKELAGVPGRFEVVDGGQNYTVIVDYAHTPDSLEN
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCHHHHH
VLKTAKQFAKGDVYCIVGCGGDRDRTKRPIMASVATKYATHAIYTSDNPRSEDPAAILDD
HHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCHHHHHHH
MVHGVSGNNYEVIIDRKEAIHHAITKAKADDIIIIAGKGHETYQIIGKDVHHFDDREVAK
HHHCCCCCCEEEEEEHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCHHHCCCHHHHHH
EAITERLNNEE
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]