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The map label for this gene is rnjA [H]

Identifier: 52141514

GI number: 52141514

Start: 3871443

End: 3873110

Strand: Direct

Name: rnjA [H]

Synonym: BCZK3735

Alternate gene names: 52141514

Gene position: 3871443-3873110 (Clockwise)

Preceding gene: 52141513

Following gene: 52141504

Centisome position: 73.03

GC content: 36.51

Gene sequence:

>1668_bases
ATGAAATTTCTAAAGAATGATCAAGCAGCCGTTTTTGCTTTAGGTGGCCTTGGTGAAATCGGTAAAAATACATATGCTGT
TCAATTTCAAGATGAAATTATTATAATTGACGCTGGAATTAAATTTCCAGAAGACGAACTCCTCGGAATCGATTATGTAA
TACCAGATTACACTTATTTTGTGCGAAACGAAGATAAAATTAAAGGATTATTCATTACACATGGTCACGAAGATCATATC
GGTGGAATTCCTTACTTATTACGTCAAGTGAACATTCCGATTTATGGCGGAAAATTAGCAATTGCACTAATCAAAAACAA
ATTAGAAGAGCACGGATTACTTCGTAAAGCAAAACTTTATGAAATTCAAGAAGATGATGTTATTAAATTTAAAAAGACAT
CTGTTTCCTTCTTCCGTACAACTCACAGTATCCCAGATTCATACGGAGTTGTTGTGAAAACACCTCAAGGACAAGTGGTT
CATACTGGAGATTTCAAATTTGATTTCACACCAGTTGGTGAACCAGCGGACTTAACAAAAATGGCTGAAATCGGAAAAGA
CGGTGTACTTTGTCTCTTATCTGACAGTACAAACAGTGAAGTTCCAAACTTTACAATGTCTGAGCGCCGTGTTGGTGATA
GTATTCAAGATATTTTCCGCAAAGTAGAAGGTCGTATTATCTTCGCAACCTTTGCATCAAATATCCATCGTTTGCAACAA
GTTGTTGAAGCGGCTGTTGAAAATAATCGTAAAGTGGCTGTATTTGGCCGAAGTATGGAAGCAGCGATTGAAATCGGACA
AAACCTCGGTTATATTCGCTGTCCGAAAGATACATTCATAGATGCATCTCAATTAAATCGTCTACCAGCTAATAAAGTTG
TTATTTTATGTACAGGTAGTCAAGGGGAGCCAATGGCAGCACTTTCACGTATTGCTAATGGTACTCATCGTCAAATTCAA
ATCATTCCTGGCGACACGGTTGTTTTCTCTTCTTCACCAATCCCTGGTAATACAATTAGTGTAAGCCGTACAATTAATAT
GTTGTACCGTGCGGGTGCTGATGTAATCCACGGAAAACTGTCAAACATTCATACGAGTGGACACGGTGGACAAGAAGAAC
AAAAACTAATGCTTCGTCTAATTAAACCGAAGTATTTCATGCCAATTCATGGTGAATATCGTATGCAACGTATGCATATG
AAGTTAGCAAATGATTGTGGTATTCCAGAAGAAAATTGTTTCATTATGGATAATGGTGATGTTCTTGCCTTACGTTCAGA
TGAAGCAAGTGTAGCTGGTAAAATACCATCTGGATCTGTCTATATTGACGGAAATGGTATTGGAGATATCGGTAATATCG
TATTACGCGATCGTCGTATCCTTTCTGAAGAAGGACTTGTTATTGTAGTTGTAAGCATTGATATGAAAGAATTTAAAGTT
GCAGCAGGGCCTGATATTATCTCACGTGGTTTCGTTTATATGCGCGAAAGTAGTGATTTAATTAATGATGCTCAAACATT
AATTACAACTCATTTAGAAAAAGTAATGGAACGTAAAACAACACAATGGTCTGAAATTAAAAATGAAATTACAGACACAT
TAGCACCATTCCTATATGAGAAAACGAAACGTCGCCCAATGATTTTACCAATCATTATGGAAATATAA

Upstream 100 bases:

>100_bases
TTCGCATATAATATCGAATTCGTTCAGTCTGTGACTGGCAATCATCTTGAATATGAAAAAGCAAACGCTGATTTAACATT
AGCGGAAATCGTATAGTCAT

Downstream 100 bases:

>100_bases
GAAAAAGGACAATACCTTTATGGTACTGTCCTTTTCTCTTATCTTAAAAAGTGCTTGAAACGATCTACTTCATCGTCATG
ACCAATTACAATTAATATAT

Product: metallo-beta-lactamase superfamily hydrolase

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MKFLKNDQAAVFALGGLGEIGKNTYAVQFQDEIIIIDAGIKFPEDELLGIDYVIPDYTYFVRNEDKIKGLFITHGHEDHI
GGIPYLLRQVNIPIYGGKLAIALIKNKLEEHGLLRKAKLYEIQEDDVIKFKKTSVSFFRTTHSIPDSYGVVVKTPQGQVV
HTGDFKFDFTPVGEPADLTKMAEIGKDGVLCLLSDSTNSEVPNFTMSERRVGDSIQDIFRKVEGRIIFATFASNIHRLQQ
VVEAAVENNRKVAVFGRSMEAAIEIGQNLGYIRCPKDTFIDASQLNRLPANKVVILCTGSQGEPMAALSRIANGTHRQIQ
IIPGDTVVFSSSPIPGNTISVSRTINMLYRAGADVIHGKLSNIHTSGHGGQEEQKLMLRLIKPKYFMPIHGEYRMQRMHM
KLANDCGIPEENCFIMDNGDVLALRSDEASVAGKIPSGSVYIDGNGIGDIGNIVLRDRRILSEEGLVIVVVSIDMKEFKV
AAGPDIISRGFVYMRESSDLINDAQTLITTHLEKVMERKTTQWSEIKNEITDTLAPFLYEKTKRRPMILPIIMEI

Sequences:

>Translated_555_residues
MKFLKNDQAAVFALGGLGEIGKNTYAVQFQDEIIIIDAGIKFPEDELLGIDYVIPDYTYFVRNEDKIKGLFITHGHEDHI
GGIPYLLRQVNIPIYGGKLAIALIKNKLEEHGLLRKAKLYEIQEDDVIKFKKTSVSFFRTTHSIPDSYGVVVKTPQGQVV
HTGDFKFDFTPVGEPADLTKMAEIGKDGVLCLLSDSTNSEVPNFTMSERRVGDSIQDIFRKVEGRIIFATFASNIHRLQQ
VVEAAVENNRKVAVFGRSMEAAIEIGQNLGYIRCPKDTFIDASQLNRLPANKVVILCTGSQGEPMAALSRIANGTHRQIQ
IIPGDTVVFSSSPIPGNTISVSRTINMLYRAGADVIHGKLSNIHTSGHGGQEEQKLMLRLIKPKYFMPIHGEYRMQRMHM
KLANDCGIPEENCFIMDNGDVLALRSDEASVAGKIPSGSVYIDGNGIGDIGNIVLRDRRILSEEGLVIVVVSIDMKEFKV
AAGPDIISRGFVYMRESSDLINDAQTLITTHLEKVMERKTTQWSEIKNEITDTLAPFLYEKTKRRPMILPIIMEI
>Mature_555_residues
MKFLKNDQAAVFALGGLGEIGKNTYAVQFQDEIIIIDAGIKFPEDELLGIDYVIPDYTYFVRNEDKIKGLFITHGHEDHI
GGIPYLLRQVNIPIYGGKLAIALIKNKLEEHGLLRKAKLYEIQEDDVIKFKKTSVSFFRTTHSIPDSYGVVVKTPQGQVV
HTGDFKFDFTPVGEPADLTKMAEIGKDGVLCLLSDSTNSEVPNFTMSERRVGDSIQDIFRKVEGRIIFATFASNIHRLQQ
VVEAAVENNRKVAVFGRSMEAAIEIGQNLGYIRCPKDTFIDASQLNRLPANKVVILCTGSQGEPMAALSRIANGTHRQIQ
IIPGDTVVFSSSPIPGNTISVSRTINMLYRAGADVIHGKLSNIHTSGHGGQEEQKLMLRLIKPKYFMPIHGEYRMQRMHM
KLANDCGIPEENCFIMDNGDVLALRSDEASVAGKIPSGSVYIDGNGIGDIGNIVLRDRRILSEEGLVIVVVSIDMKEFKV
AAGPDIISRGFVYMRESSDLINDAQTLITTHLEKVMERKTTQWSEIKNEITDTLAPFLYEKTKRRPMILPIIMEI

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61800; Mature: 61800

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLKNDQAAVFALGGLGEIGKNTYAVQFQDEIIIIDAGIKFPEDELLGIDYVIPDYTYF
CCCCCCCCEEEEEECCCCCCCCCEEEEEEECEEEEEECCCCCCCHHHEEEEEECCCEEEE
VRNEDKIKGLFITHGHEDHIGGIPYLLRQVNIPIYGGKLAIALIKNKLEEHGLLRKAKLY
EECCCCEEEEEEECCCCCCCCCHHHHHHHCCCCEECCCEEEHHHHHHHHHCCCHHHHHEE
EIQEDDVIKFKKTSVSFFRTTHSIPDSYGVVVKTPQGQVVHTGDFKFDFTPVGEPADLTK
ECCCCCEEEEEECCEEEEEEECCCCCCCCEEEECCCCCEEECCCEEEEECCCCCCHHHHH
MAEIGKDGVLCLLSDSTNSEVPNFTMSERRVGDSIQDIFRKVEGRIIFATFASNIHRLQQ
HHHCCCCCEEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHCCEEEEEEHHHHHHHHHH
VVEAAVENNRKVAVFGRSMEAAIEIGQNLGYIRCPKDTFIDASQLNRLPANKVVILCTGS
HHHHHHHCCCEEEEECCCHHHHHHHHCCCCEEECCCCCCCCHHHHCCCCCCCEEEEEECC
QGEPMAALSRIANGTHRQIQIIPGDTVVFSSSPIPGNTISVSRTINMLYRAGADVIHGKL
CCCHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCCEEEHHHHHHHHHHCCCHHEECHH
SNIHTSGHGGQEEQKLMLRLIKPKYFMPIHGEYRMQRMHMKLANDCGIPEENCFIMDNGD
HCEEECCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCC
VLALRSDEASVAGKIPSGSVYIDGNGIGDIGNIVLRDRRILSEEGLVIVVVSIDMKEFKV
EEEEECCCCCEECCCCCCEEEECCCCCCCHHHHEECCCHHHCCCCEEEEEEEECHHHEEE
AAGPDIISRGFVYMRESSDLINDAQTLITTHLEKVMERKTTQWSEIKNEITDTLAPFLYE
ECCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KTKRRPMILPIIMEI
HHCCCCEEEEEEEEC
>Mature Secondary Structure
MKFLKNDQAAVFALGGLGEIGKNTYAVQFQDEIIIIDAGIKFPEDELLGIDYVIPDYTYF
CCCCCCCCEEEEEECCCCCCCCCEEEEEEECEEEEEECCCCCCCHHHEEEEEECCCEEEE
VRNEDKIKGLFITHGHEDHIGGIPYLLRQVNIPIYGGKLAIALIKNKLEEHGLLRKAKLY
EECCCCEEEEEEECCCCCCCCCHHHHHHHCCCCEECCCEEEHHHHHHHHHCCCHHHHHEE
EIQEDDVIKFKKTSVSFFRTTHSIPDSYGVVVKTPQGQVVHTGDFKFDFTPVGEPADLTK
ECCCCCEEEEEECCEEEEEEECCCCCCCCEEEECCCCCEEECCCEEEEECCCCCCHHHHH
MAEIGKDGVLCLLSDSTNSEVPNFTMSERRVGDSIQDIFRKVEGRIIFATFASNIHRLQQ
HHHCCCCCEEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHCCEEEEEEHHHHHHHHHH
VVEAAVENNRKVAVFGRSMEAAIEIGQNLGYIRCPKDTFIDASQLNRLPANKVVILCTGS
HHHHHHHCCCEEEEECCCHHHHHHHHCCCCEEECCCCCCCCHHHHCCCCCCCEEEEEECC
QGEPMAALSRIANGTHRQIQIIPGDTVVFSSSPIPGNTISVSRTINMLYRAGADVIHGKL
CCCHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCCEEEHHHHHHHHHHCCCHHEECHH
SNIHTSGHGGQEEQKLMLRLIKPKYFMPIHGEYRMQRMHMKLANDCGIPEENCFIMDNGD
HCEEECCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCC
VLALRSDEASVAGKIPSGSVYIDGNGIGDIGNIVLRDRRILSEEGLVIVVVSIDMKEFKV
EEEEECCCCCEECCCCCCEEEECCCCCCCHHHHEECCCHHHCCCCEEEEEEEECHHHEEE
AAGPDIISRGFVYMRESSDLINDAQTLITTHLEKVMERKTTQWSEIKNEITDTLAPFLYE
ECCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KTKRRPMILPIIMEI
HHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]