| Definition | Bacillus cereus E33L, complete genome. |
|---|---|
| Accession | NC_006274 |
| Length | 5,300,915 |
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The map label for this gene is yxjF [H]
Identifier: 52141459
GI number: 52141459
Start: 3923786
End: 3924505
Strand: Direct
Name: yxjF [H]
Synonym: BCZK3788
Alternate gene names: 52141459
Gene position: 3923786-3924505 (Clockwise)
Preceding gene: 52141467
Following gene: 52141457
Centisome position: 74.02
GC content: 38.06
Gene sequence:
>720_bases ATGGGAAACGCTTTTGCAAAAGAAGGTGCAAAAGTTGTTATTACCGATCGTCTTGAAGAGCGTGCAAAAGAAGCTGCTGA ACAACTACAAAAAGAGGGCTTTCAAGCTATCGGCTTAAAATGTGATGTTACATCCGAAGAAGAAATAGAAGCATCCATTT CCCAAACTGTTAATCATTTCGGTTCATTAGACATATTAATTAATAACGCAGGAATGCAACACGTTTCACCAATTGAAGAT TTTCCAACCGAAAAATTCGAGCTACTAATTAAAATTATGCAAATCGCTCCTTTTATCGCAATTAAGCATGCCTTCCCTAT TATGAAAAAACAAAAATATGGCCGTATTATAAATGTAGCTTCCATTAATGGCCTCGTTGGATTCGCTGGAAAAGCTGCTT ATAATAGCGCAAAACATGGCGTAATTGGATTAACAAAAGTTGCTGCTTTAGAAGGGGCTACTCACCGAATTACTGTTAAT GCACTTTGTCCCGGTTATGTCGATACCCCTCTCGTAAGGAATCAATTACAAGACTTGGCCACTACACGAAATGTACCACT TGAAAATGTTTTAGAGGATGTCATTTATCCACTTGTCCCACAAAAACGCTTATTACAAGTACAAGAGATTGCTGATTATG CGATGTTTTTAGCGAGCGAAAAAGCTAAAGGTATAACTGGACAAGCTGTCGTTATCGATGGTGGTTATACCGCTCAATAA
Upstream 100 bases:
>100_bases TAGAGCAATATGAGAACTTTATTACGAAGGGATGTTTTTCATCATGGTTACAAATCGAGTTGTTTTTTTAACAGGTGCTG CAAGTGGTATTGGATATGAA
Downstream 100 bases:
>100_bases AAAATAAAGTTTGCTCACGAAGAGCAAGCTTTATTTTTTATCGCGAGGATCATCGTACTTTAGTAATTGCCTTGGCAACT TCTCTATTGCAACAATTACC
Product: 3-hydroxybutyrate dehydrogenase
Products: acetoacetate; NADH; H+
Alternate protein names: NA
Number of amino acids: Translated: 239; Mature: 238
Protein sequence:
>239_residues MGNAFAKEGAKVVITDRLEERAKEAAEQLQKEGFQAIGLKCDVTSEEEIEASISQTVNHFGSLDILINNAGMQHVSPIED FPTEKFELLIKIMQIAPFIAIKHAFPIMKKQKYGRIINVASINGLVGFAGKAAYNSAKHGVIGLTKVAALEGATHRITVN ALCPGYVDTPLVRNQLQDLATTRNVPLENVLEDVIYPLVPQKRLLQVQEIADYAMFLASEKAKGITGQAVVIDGGYTAQ
Sequences:
>Translated_239_residues MGNAFAKEGAKVVITDRLEERAKEAAEQLQKEGFQAIGLKCDVTSEEEIEASISQTVNHFGSLDILINNAGMQHVSPIED FPTEKFELLIKIMQIAPFIAIKHAFPIMKKQKYGRIINVASINGLVGFAGKAAYNSAKHGVIGLTKVAALEGATHRITVN ALCPGYVDTPLVRNQLQDLATTRNVPLENVLEDVIYPLVPQKRLLQVQEIADYAMFLASEKAKGITGQAVVIDGGYTAQ >Mature_238_residues GNAFAKEGAKVVITDRLEERAKEAAEQLQKEGFQAIGLKCDVTSEEEIEASISQTVNHFGSLDILINNAGMQHVSPIEDF PTEKFELLIKIMQIAPFIAIKHAFPIMKKQKYGRIINVASINGLVGFAGKAAYNSAKHGVIGLTKVAALEGATHRITVNA LCPGYVDTPLVRNQLQDLATTRNVPLENVLEDVIYPLVPQKRLLQVQEIADYAMFLASEKAKGITGQAVVIDGGYTAQ
Specific function: Catalyzes A Reversible Reduction Of 5-Ketoglutanate To Form D-Gluconate. Dependent On Nadp, Almost Inactive With NAD. [C]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI59889578, Length=234, Percent_Identity=32.4786324786325, Blast_Score=113, Evalue=2e-25, Organism=Homo sapiens, GI66933014, Length=238, Percent_Identity=35.2941176470588, Blast_Score=112, Evalue=3e-25, Organism=Homo sapiens, GI32483357, Length=236, Percent_Identity=30.5084745762712, Blast_Score=108, Evalue=5e-24, Organism=Homo sapiens, GI19923817, Length=244, Percent_Identity=29.0983606557377, Blast_Score=104, Evalue=9e-23, Organism=Homo sapiens, GI40254992, Length=231, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=5e-21, Organism=Homo sapiens, GI31542939, Length=243, Percent_Identity=27.5720164609054, Blast_Score=90, Evalue=1e-18, Organism=Homo sapiens, GI4504505, Length=170, Percent_Identity=31.1764705882353, Blast_Score=89, Evalue=4e-18, Organism=Homo sapiens, GI15277342, Length=245, Percent_Identity=29.7959183673469, Blast_Score=83, Evalue=3e-16, Organism=Homo sapiens, GI5031737, Length=231, Percent_Identity=25.1082251082251, Blast_Score=82, Evalue=4e-16, Organism=Homo sapiens, GI4503301, Length=229, Percent_Identity=26.6375545851528, Blast_Score=82, Evalue=4e-16, Organism=Homo sapiens, GI126723750, Length=240, Percent_Identity=26.6666666666667, Blast_Score=81, Evalue=7e-16, Organism=Homo sapiens, GI33667109, Length=166, Percent_Identity=28.9156626506024, Blast_Score=79, Evalue=3e-15, Organism=Homo sapiens, GI40254986, Length=168, Percent_Identity=30.3571428571429, Blast_Score=79, Evalue=4e-15, Organism=Homo sapiens, GI142976729, Length=204, Percent_Identity=26.9607843137255, Blast_Score=77, Evalue=1e-14, Organism=Homo sapiens, GI10190704, Length=232, Percent_Identity=28.448275862069, Blast_Score=75, Evalue=4e-14, Organism=Homo sapiens, GI224922801, Length=158, Percent_Identity=32.2784810126582, Blast_Score=75, Evalue=6e-14, Organism=Homo sapiens, GI126723191, Length=159, Percent_Identity=30.188679245283, Blast_Score=74, Evalue=9e-14, Organism=Homo sapiens, GI7706318, Length=177, Percent_Identity=28.8135593220339, Blast_Score=72, Evalue=5e-13, Organism=Homo sapiens, GI20149619, Length=178, Percent_Identity=27.5280898876405, Blast_Score=72, Evalue=6e-13, Organism=Homo sapiens, GI4503817, Length=190, Percent_Identity=25.7894736842105, Blast_Score=71, Evalue=9e-13, Organism=Homo sapiens, GI210032110, Length=210, Percent_Identity=26.1904761904762, Blast_Score=69, Evalue=4e-12, Organism=Homo sapiens, GI4758504, Length=200, Percent_Identity=27.5, Blast_Score=66, Evalue=3e-11, Organism=Escherichia coli, GI1790717, Length=235, Percent_Identity=30.6382978723404, Blast_Score=125, Evalue=4e-30, Organism=Escherichia coli, GI1787335, Length=231, Percent_Identity=33.7662337662338, Blast_Score=116, Evalue=1e-27, Organism=Escherichia coli, GI87082100, Length=235, Percent_Identity=32.7659574468085, Blast_Score=114, Evalue=4e-27, Organism=Escherichia coli, GI87082160, Length=233, Percent_Identity=31.3304721030043, Blast_Score=113, Evalue=1e-26, Organism=Escherichia coli, GI1788459, Length=234, Percent_Identity=31.6239316239316, Blast_Score=110, Evalue=7e-26, Organism=Escherichia coli, GI1789057, Length=238, Percent_Identity=30.672268907563, Blast_Score=101, Evalue=4e-23, Organism=Escherichia coli, GI1789208, Length=240, Percent_Identity=27.9166666666667, Blast_Score=91, Evalue=9e-20, Organism=Escherichia coli, GI1787905, Length=239, Percent_Identity=29.2887029288703, Blast_Score=89, Evalue=2e-19, Organism=Escherichia coli, GI2367365, Length=235, Percent_Identity=30.2127659574468, Blast_Score=74, Evalue=1e-14, Organism=Escherichia coli, GI1786812, Length=176, Percent_Identity=27.2727272727273, Blast_Score=64, Evalue=8e-12, Organism=Escherichia coli, GI2367175, Length=237, Percent_Identity=24.8945147679325, Blast_Score=63, Evalue=1e-11, Organism=Escherichia coli, GI1789378, Length=235, Percent_Identity=26.8085106382979, Blast_Score=61, Evalue=6e-11, Organism=Caenorhabditis elegans, GI71994600, Length=235, Percent_Identity=33.1914893617021, Blast_Score=99, Evalue=2e-21, Organism=Caenorhabditis elegans, GI17555706, Length=240, Percent_Identity=30, Blast_Score=94, Evalue=6e-20, Organism=Caenorhabditis elegans, GI25147288, Length=236, Percent_Identity=30.0847457627119, Blast_Score=94, Evalue=8e-20, Organism=Caenorhabditis elegans, GI71994604, Length=234, Percent_Identity=32.0512820512821, Blast_Score=91, Evalue=4e-19, Organism=Caenorhabditis elegans, GI17568967, Length=169, Percent_Identity=31.9526627218935, Blast_Score=86, Evalue=1e-17, Organism=Caenorhabditis elegans, GI17564282, Length=195, Percent_Identity=30.7692307692308, Blast_Score=86, Evalue=1e-17, Organism=Caenorhabditis elegans, GI17562910, Length=243, Percent_Identity=30.4526748971193, Blast_Score=84, Evalue=5e-17, Organism=Caenorhabditis elegans, GI17560676, Length=244, Percent_Identity=27.0491803278689, Blast_Score=84, Evalue=9e-17, Organism=Caenorhabditis elegans, GI17561402, Length=238, Percent_Identity=29.8319327731092, Blast_Score=82, Evalue=3e-16, Organism=Caenorhabditis elegans, GI17559104, Length=249, Percent_Identity=30.1204819277108, Blast_Score=81, Evalue=5e-16, Organism=Caenorhabditis elegans, GI17558006, Length=241, Percent_Identity=30.2904564315353, Blast_Score=81, Evalue=6e-16, Organism=Caenorhabditis elegans, GI17560150, Length=247, Percent_Identity=30.7692307692308, Blast_Score=80, Evalue=6e-16, Organism=Caenorhabditis elegans, GI193203115, Length=171, Percent_Identity=29.2397660818713, Blast_Score=80, Evalue=7e-16, Organism=Caenorhabditis elegans, GI193203117, Length=171, Percent_Identity=29.2397660818713, Blast_Score=80, Evalue=7e-16, Organism=Caenorhabditis elegans, GI17538182, Length=184, Percent_Identity=29.3478260869565, Blast_Score=79, Evalue=3e-15, Organism=Caenorhabditis elegans, GI17544670, Length=244, Percent_Identity=26.2295081967213, Blast_Score=78, Evalue=3e-15, Organism=Caenorhabditis elegans, GI71982365, Length=169, Percent_Identity=30.1775147928994, Blast_Score=75, Evalue=2e-14, Organism=Caenorhabditis elegans, GI17538486, Length=253, Percent_Identity=28.4584980237154, Blast_Score=75, Evalue=3e-14, Organism=Caenorhabditis elegans, GI17562908, Length=245, Percent_Identity=28.1632653061224, Blast_Score=74, Evalue=6e-14, Organism=Caenorhabditis elegans, GI17560220, Length=245, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=3e-13, Organism=Caenorhabditis elegans, GI17562990, Length=249, Percent_Identity=29.3172690763052, Blast_Score=72, Evalue=3e-13, Organism=Caenorhabditis elegans, GI193204405, Length=252, Percent_Identity=29.7619047619048, Blast_Score=71, Evalue=5e-13, Organism=Caenorhabditis elegans, GI17562904, Length=249, Percent_Identity=30.5220883534137, Blast_Score=70, Evalue=8e-13, Organism=Caenorhabditis elegans, GI17531453, Length=256, Percent_Identity=27.34375, Blast_Score=69, Evalue=2e-12, Organism=Caenorhabditis elegans, GI17551412, Length=242, Percent_Identity=28.099173553719, Blast_Score=68, Evalue=4e-12, Organism=Caenorhabditis elegans, GI17508895, Length=174, Percent_Identity=26.4367816091954, Blast_Score=68, Evalue=4e-12, Organism=Caenorhabditis elegans, GI17562906, Length=252, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=5e-12, Organism=Caenorhabditis elegans, GI17560332, Length=257, Percent_Identity=29.5719844357977, Blast_Score=65, Evalue=2e-11, Organism=Caenorhabditis elegans, GI17565030, Length=256, Percent_Identity=28.515625, Blast_Score=64, Evalue=5e-11, Organism=Caenorhabditis elegans, GI17538480, Length=242, Percent_Identity=27.6859504132231, Blast_Score=64, Evalue=5e-11, Organism=Saccharomyces cerevisiae, GI6322861, Length=209, Percent_Identity=32.0574162679426, Blast_Score=91, Evalue=2e-19, Organism=Saccharomyces cerevisiae, GI6324126, Length=231, Percent_Identity=26.8398268398268, Blast_Score=73, Evalue=3e-14, Organism=Drosophila melanogaster, GI21355319, Length=236, Percent_Identity=31.3559322033898, Blast_Score=110, Evalue=7e-25, Organism=Drosophila melanogaster, GI28571526, Length=235, Percent_Identity=33.6170212765957, Blast_Score=97, Evalue=7e-21, Organism=Drosophila melanogaster, GI21357041, Length=237, Percent_Identity=31.6455696202532, Blast_Score=97, Evalue=1e-20, Organism=Drosophila melanogaster, GI24639444, Length=234, Percent_Identity=29.9145299145299, Blast_Score=97, Evalue=1e-20, Organism=Drosophila melanogaster, GI24644339, Length=235, Percent_Identity=33.1914893617021, Blast_Score=94, Evalue=1e-19, Organism=Drosophila melanogaster, GI23397609, Length=231, Percent_Identity=32.034632034632, Blast_Score=94, Evalue=1e-19, Organism=Drosophila melanogaster, GI18859837, Length=169, Percent_Identity=33.1360946745562, Blast_Score=91, Evalue=8e-19, Organism=Drosophila melanogaster, GI24581726, Length=169, Percent_Identity=28.9940828402367, Blast_Score=84, Evalue=9e-17, Organism=Drosophila melanogaster, GI17737361, Length=180, Percent_Identity=28.8888888888889, Blast_Score=80, Evalue=8e-16, Organism=Drosophila melanogaster, GI24643142, Length=175, Percent_Identity=30.2857142857143, Blast_Score=78, Evalue=4e-15, Organism=Drosophila melanogaster, GI24644337, Length=234, Percent_Identity=29.4871794871795, Blast_Score=77, Evalue=8e-15, Organism=Drosophila melanogaster, GI21357673, Length=168, Percent_Identity=27.9761904761905, Blast_Score=70, Evalue=9e-13, Organism=Drosophila melanogaster, GI24640492, Length=180, Percent_Identity=25, Blast_Score=68, Evalue=5e-12, Organism=Drosophila melanogaster, GI24641232, Length=226, Percent_Identity=25.6637168141593, Blast_Score=65, Evalue=4e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR011294 - InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 - InterPro: IPR020904 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.1.1.30
Molecular weight: Translated: 25988; Mature: 25857
Theoretical pI: Translated: 6.36; Mature: 6.36
Prosite motif: PS00061 ADH_SHORT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 2.1 %Met (Translated Protein) 2.9 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 1.7 %Met (Mature Protein) 2.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MGNAFAKEGAKVVITDRLEERAKEAAEQLQKEGFQAIGLKCDVTSEEEIEASISQTVNHF CCCCCHHCCCEEEEHHHHHHHHHHHHHHHHHCCHHHCCEEECCCCHHHHHHHHHHHHHHC GSLDILINNAGMQHVSPIEDFPTEKFELLIKIMQIAPFIAIKHAFPIMKKQKYGRIINVA CCEEEEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE SINGLVGFAGKAAYNSAKHGVIGLTKVAALEGATHRITVNALCPGYVDTPLVRNQLQDLA ECCCHHHCCCHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHH TTRNVPLENVLEDVIYPLVPQKRLLQVQEIADYAMFLASEKAKGITGQAVVIDGGYTAQ HHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCC >Mature Secondary Structure GNAFAKEGAKVVITDRLEERAKEAAEQLQKEGFQAIGLKCDVTSEEEIEASISQTVNHF CCCCHHCCCEEEEHHHHHHHHHHHHHHHHHCCHHHCCEEECCCCHHHHHHHHHHHHHHC GSLDILINNAGMQHVSPIEDFPTEKFELLIKIMQIAPFIAIKHAFPIMKKQKYGRIINVA CCEEEEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE SINGLVGFAGKAAYNSAKHGVIGLTKVAALEGATHRITVNALCPGYVDTPLVRNQLQDLA ECCCHHHCCCHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHH TTRNVPLENVLEDVIYPLVPQKRLLQVQEIADYAMFLASEKAKGITGQAVVIDGGYTAQ HHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: (R)-3-hydroxybutanoate; NAD+
Specific reaction: (R)-3-hydroxybutanoate + NAD+ = acetoacetate + NADH + H+
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 8969509; 9384377 [H]