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The map label for this gene is pbuO [H]

Identifier: 52141409

GI number: 52141409

Start: 3969485

End: 3970774

Strand: Direct

Name: pbuO [H]

Synonym: BCZK3840

Alternate gene names: 52141409

Gene position: 3969485-3970774 (Clockwise)

Preceding gene: 52141412

Following gene: 52141398

Centisome position: 74.88

GC content: 40.0

Gene sequence:

>1290_bases
ATGAAAGGAATACTTGAAAGAACTTTTAAATTAGGTTTACACGAAACATCACCAAAACAAGAAGTTTTAGCTGGAGTTAC
GTCATTTTTCACAATCGTATACATTATGATTGTAAATGCATCCATTTTATCAGATGCTGGCATTCCTCTTGAAGCTGGAA
TTTTAGCAACTGTTTTCAGTTCATTTGTCGGATGTCTGCTCATGGCATTTTGGGCAAATGCACCCGCTATTCTTGTCCCT
GGTATGGGTGTAAATGCATTCTTCACGTACACTGCTGTCCATACGCTCGGATTAACTTGGCAGGAAGCATTAGCAGCTGT
CTTCATCTCTGGTATTATTTTTGCAATTGCTGCGTTTACACCAATCGCTCGCGTGCTTTCCGTATCGATTCCAAAGTCAT
TAAAGGAAGCTATTACTGTCGGTATCGGATTGTTTTTAGCGTTTATCGGATTGCAAAAAGGTGGTTTAGTCGTTTCGAAT
CCAAATACTGCTGTTGCAATGGGGAAATTAAGTAACCCTGTCGTTCTTGCGACCGTGCTTACTCTTATCGTTGCACTCGT
ATTATTTATTCGTAATGTACGTGGAAACTTTTTATGGACGATTGCAATAGGAACTGGTATCGCATGGCTATTTGGTCTTG
TGGATACAAGTCAAATTGGAAATAGTTCATTTTCATTCGCTAATTACGGCGATGTGTTTGGAGCTATGTCATTTGGCAAA
CTCTCTTCCTTACCGTTTTGGATTGCAACATTCTCTTTAAGCATGGTGCTTATTTTTGAGAACATGGGACTTCTGCATGG
TTTATTAGAAGATGACCGTAAATTCCCACGTGCTTACCAAGCCAATGCAATTTCAGCAATGACATGTGGTCTATTTGGCA
CAAGCCCTACAGTATCAACAGTAGAGAGTGCCGCAGGTATTACTGCAGGCGGAAAGACAGGTCTGACGTCTATCGTTACA
GGGCTGTTATTCTTTGCATCACTGTTTGCACTTCCGTTTGTCAAACTAATTCCTGATAGTGCCATTGCACCAATCCTAAT
TATTATTGGCGGCCTGATGATTACAAGCATTCAACAAATTCCTCTGAACGATTTTTCAGAAGGATTTCCAGCGTTCTTAA
TTATCGTCATGATTCCGCTCACATATAGTATCGCTGATGGCATTGCGTTCGGATTTATTGCTTATCCTATTTTAAAAGTT
GCTCTTGGAAAGCGTAAAGAAGTCGCACCGTCTATGTATATCATTACATGCTTATTCTTAGCCATGTTCGTATTACATGC
TATTGGTTAA

Upstream 100 bases:

>100_bases
CTTGTTGTAAAAACATGATGTTCTGATATAATAAAGGACGGTGAGCTCTTACGAAAGAGATATCACGGGTGGGAGAGGGA
TATGAAAAAGAAGGTTTAAC

Downstream 100 bases:

>100_bases
GTAAAAACACCGAGGAAAATTCCTCGGTGTTTTTTTTACTTAAACCATCCTTTTTTATAAAACCAAACCATCATGCCACC
GCCAATTAAAGCCATGATAA

Product: xanthine/uracil permease family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKGILERTFKLGLHETSPKQEVLAGVTSFFTIVYIMIVNASILSDAGIPLEAGILATVFSSFVGCLLMAFWANAPAILVP
GMGVNAFFTYTAVHTLGLTWQEALAAVFISGIIFAIAAFTPIARVLSVSIPKSLKEAITVGIGLFLAFIGLQKGGLVVSN
PNTAVAMGKLSNPVVLATVLTLIVALVLFIRNVRGNFLWTIAIGTGIAWLFGLVDTSQIGNSSFSFANYGDVFGAMSFGK
LSSLPFWIATFSLSMVLIFENMGLLHGLLEDDRKFPRAYQANAISAMTCGLFGTSPTVSTVESAAGITAGGKTGLTSIVT
GLLFFASLFALPFVKLIPDSAIAPILIIIGGLMITSIQQIPLNDFSEGFPAFLIIVMIPLTYSIADGIAFGFIAYPILKV
ALGKRKEVAPSMYIITCLFLAMFVLHAIG

Sequences:

>Translated_429_residues
MKGILERTFKLGLHETSPKQEVLAGVTSFFTIVYIMIVNASILSDAGIPLEAGILATVFSSFVGCLLMAFWANAPAILVP
GMGVNAFFTYTAVHTLGLTWQEALAAVFISGIIFAIAAFTPIARVLSVSIPKSLKEAITVGIGLFLAFIGLQKGGLVVSN
PNTAVAMGKLSNPVVLATVLTLIVALVLFIRNVRGNFLWTIAIGTGIAWLFGLVDTSQIGNSSFSFANYGDVFGAMSFGK
LSSLPFWIATFSLSMVLIFENMGLLHGLLEDDRKFPRAYQANAISAMTCGLFGTSPTVSTVESAAGITAGGKTGLTSIVT
GLLFFASLFALPFVKLIPDSAIAPILIIIGGLMITSIQQIPLNDFSEGFPAFLIIVMIPLTYSIADGIAFGFIAYPILKV
ALGKRKEVAPSMYIITCLFLAMFVLHAIG
>Mature_429_residues
MKGILERTFKLGLHETSPKQEVLAGVTSFFTIVYIMIVNASILSDAGIPLEAGILATVFSSFVGCLLMAFWANAPAILVP
GMGVNAFFTYTAVHTLGLTWQEALAAVFISGIIFAIAAFTPIARVLSVSIPKSLKEAITVGIGLFLAFIGLQKGGLVVSN
PNTAVAMGKLSNPVVLATVLTLIVALVLFIRNVRGNFLWTIAIGTGIAWLFGLVDTSQIGNSSFSFANYGDVFGAMSFGK
LSSLPFWIATFSLSMVLIFENMGLLHGLLEDDRKFPRAYQANAISAMTCGLFGTSPTVSTVESAAGITAGGKTGLTSIVT
GLLFFASLFALPFVKLIPDSAIAPILIIIGGLMITSIQQIPLNDFSEGFPAFLIIVMIPLTYSIADGIAFGFIAYPILKV
ALGKRKEVAPSMYIITCLFLAMFVLHAIG

Specific function: Involved in the uptake of the purine bases hypoxanthine and guanine. May work at purine concentrations higher than 100 uM [H]

COG id: COG2252

COG function: function code R; Permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. AzgA purine transporter (TC 2.A.1.40) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790150, Length=431, Percent_Identity=35.7308584686775, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI87082309, Length=428, Percent_Identity=33.6448598130841, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI48994909, Length=441, Percent_Identity=31.7460317460317, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1790499, Length=422, Percent_Identity=31.2796208530806, Blast_Score=160, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 45408; Mature: 45408

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGILERTFKLGLHETSPKQEVLAGVTSFFTIVYIMIVNASILSDAGIPLEAGILATVFS
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SFVGCLLMAFWANAPAILVPGMGVNAFFTYTAVHTLGLTWQEALAAVFISGIIFAIAAFT
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
PIARVLSVSIPKSLKEAITVGIGLFLAFIGLQKGGLVVSNPNTAVAMGKLSNPVVLATVL
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCCHHHHHHH
TLIVALVLFIRNVRGNFLWTIAIGTGIAWLFGLVDTSQIGNSSFSFANYGDVFGAMSFGK
HHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHH
LSSLPFWIATFSLSMVLIFENMGLLHGLLEDDRKFPRAYQANAISAMTCGLFGTSPTVST
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHCCCCCCCCHHH
VESAAGITAGGKTGLTSIVTGLLFFASLFALPFVKLIPDSAIAPILIIIGGLMITSIQQI
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
PLNDFSEGFPAFLIIVMIPLTYSIADGIAFGFIAYPILKVALGKRKEVAPSMYIITCLFL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHH
AMFVLHAIG
HHHHHHHCC
>Mature Secondary Structure
MKGILERTFKLGLHETSPKQEVLAGVTSFFTIVYIMIVNASILSDAGIPLEAGILATVFS
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SFVGCLLMAFWANAPAILVPGMGVNAFFTYTAVHTLGLTWQEALAAVFISGIIFAIAAFT
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
PIARVLSVSIPKSLKEAITVGIGLFLAFIGLQKGGLVVSNPNTAVAMGKLSNPVVLATVL
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCCHHHHHHH
TLIVALVLFIRNVRGNFLWTIAIGTGIAWLFGLVDTSQIGNSSFSFANYGDVFGAMSFGK
HHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHH
LSSLPFWIATFSLSMVLIFENMGLLHGLLEDDRKFPRAYQANAISAMTCGLFGTSPTVST
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHCCCCCCCCHHH
VESAAGITAGGKTGLTSIVTGLLFFASLFALPFVKLIPDSAIAPILIIIGGLMITSIQQI
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
PLNDFSEGFPAFLIIVMIPLTYSIADGIAFGFIAYPILKVALGKRKEVAPSMYIITCLFL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHH
AMFVLHAIG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 11591660 [H]