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The map label for this gene is ispG

Identifier: 52141219

GI number: 52141219

Start: 4138443

End: 4139579

Strand: Direct

Name: ispG

Synonym: BCZK4028

Alternate gene names: 52141219

Gene position: 4138443-4139579 (Clockwise)

Preceding gene: 52141218

Following gene: 52141215

Centisome position: 78.07

GC content: 38.35

Gene sequence:

>1137_bases
GTGAATGAAATGACTCATCGTACAAAAACACGTCCAGTTAAAGTCGGTAATTTAACAATTGGCGGTAATAATGAATTAAT
TATACAAAGTATGACAACAACAAAAACACATGATGTTGAAGCAACAGTTGCTGAAATTAAACGTTTAGAAGAAGCTGGCT
GTCAAGTCGTGCGTGTTGCTGTTCCAGACGAACGCGCAGCAAATGCCATTGCTGATATTAAAAAACAAATCAACATTCCA
CTTGTTGCTGATATTCATTTTGATTATCGCCTTGCTTTAAAAGCAATTGAAGGTGGCATTGATAAAGTACGTATCAATCC
AGGTAACATTGGCCGTCGCCATAAAGTAGAAGCTGTTGTAAATGCAGCAAAAGAACGCGGTATTCCAATCCGTATCGGTG
TAAACGCAGGTTCATTAGAACGTCACATTTTAGAAAAGTACGGATACCCAACTGCAGATGGTATGGTGGAGAGCGCCTTG
CATCATATTAAAATTTTAGAGGACTTAGATTTCCACGATATTATCGTATCTATGAAAGCCTCTGATGTTAACTTAGCAAT
TGAAGCATACGAAAAGGCTGCACGTGCTTTTGATTACCCATTACATTTAGGTATCACAGAATCTGGAACATTATTTGCCG
GAACTGTAAAAAGTGCTGCTGGTCTTGGAGCAATCTTAAATAAAGGTATCGGAAATACACTACGTATTTCACTAAGTGCT
GACCCAGTTGAAGAAGTAAAAGTTGCGCGTGAACTATTAAAGTCATTCGGCCTTGCATCTAATGCAGCAACACTTATTTC
TTGTCCAACTTGTGGTCGTATTGAAATTGATTTAATTAGCATCGCTAACGAGGTGGAAGAGTACATCTCTACACTGCAAG
TACCAATTAAAGTTGCCGTACTTGGTTGTGCTGTAAATGGTCCTGGTGAAGCTCGTGAAGCTGATATCGGTATTGCCGGT
GCACGCGGAGAAGGATTATTATTCCGTAAAGGGCAAGTTGTTCGTAAAGTACCAGAAGAAATAATGGTAGAAGAACTAAA
AAAAGAAATTGACGTAATTGCTGCTGAAATGGCTGCTGAACGAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAAG
AAACACAAGAACAATAA

Upstream 100 bases:

>100_bases
AAACCGAGCACTTCGGTTTCTATAGCCCTGTATCACATATACTATCATTGATATAAGGGACTAAATAAGAAACTTCTTTT
ATAAGGAGAGCGATCTATTC

Downstream 100 bases:

>100_bases
AAAGAAGCTCGTCAGTTCCATAACTGACGAGCTTTTACTTTGCACATTTTGGACAACGTCCGTATATTTCAAACTTATGA
CCTGTTACTTCATAACCGTT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MNEMTHRTKTRPVKVGNLTIGGNNELIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVAVPDERAANAIADIKKQINIP
LVADIHFDYRLALKAIEGGIDKVRINPGNIGRRHKVEAVVNAAKERGIPIRIGVNAGSLERHILEKYGYPTADGMVESAL
HHIKILEDLDFHDIIVSMKASDVNLAIEAYEKAARAFDYPLHLGITESGTLFAGTVKSAAGLGAILNKGIGNTLRISLSA
DPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISTLQVPIKVAVLGCAVNGPGEAREADIGIAG
ARGEGLLFRKGQVVRKVPEEIMVEELKKEIDVIAAEMAAEREKEKEKEKEKEKETQEQ

Sequences:

>Translated_378_residues
MNEMTHRTKTRPVKVGNLTIGGNNELIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVAVPDERAANAIADIKKQINIP
LVADIHFDYRLALKAIEGGIDKVRINPGNIGRRHKVEAVVNAAKERGIPIRIGVNAGSLERHILEKYGYPTADGMVESAL
HHIKILEDLDFHDIIVSMKASDVNLAIEAYEKAARAFDYPLHLGITESGTLFAGTVKSAAGLGAILNKGIGNTLRISLSA
DPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISTLQVPIKVAVLGCAVNGPGEAREADIGIAG
ARGEGLLFRKGQVVRKVPEEIMVEELKKEIDVIAAEMAAEREKEKEKEKEKEKETQEQ
>Mature_378_residues
MNEMTHRTKTRPVKVGNLTIGGNNELIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVAVPDERAANAIADIKKQINIP
LVADIHFDYRLALKAIEGGIDKVRINPGNIGRRHKVEAVVNAAKERGIPIRIGVNAGSLERHILEKYGYPTADGMVESAL
HHIKILEDLDFHDIIVSMKASDVNLAIEAYEKAARAFDYPLHLGITESGTLFAGTVKSAAGLGAILNKGIGNTLRISLSA
DPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISTLQVPIKVAVLGCAVNGPGEAREADIGIAG
ARGEGLLFRKGQVVRKVPEEIMVEELKKEIDVIAAEMAAEREKEKEKEKEKEKETQEQ

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=356, Percent_Identity=48.314606741573, Blast_Score=347, Evalue=6e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_BACCZ (Q634Q9)

Other databases:

- EMBL:   CP000001
- RefSeq:   YP_085608.1
- ProteinModelPortal:   Q634Q9
- SMR:   Q634Q9
- STRING:   Q634Q9
- EnsemblBacteria:   EBBACT00000042205
- GeneID:   3024466
- GenomeReviews:   CP000001_GR
- KEGG:   bcz:BCZK4028
- eggNOG:   COG0821
- GeneTree:   EBGT00050000002545
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- BioCyc:   BCER288681:BCE33L4028-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 41057; Mature: 41057

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEMTHRTKTRPVKVGNLTIGGNNELIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVA
CCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEE
VPDERAANAIADIKKQINIPLVADIHFDYRLALKAIEGGIDKVRINPGNIGRRHKVEAVV
CCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHH
NAAKERGIPIRIGVNAGSLERHILEKYGYPTADGMVESALHHIKILEDLDFHDIIVSMKA
HHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEEC
SDVNLAIEAYEKAARAFDYPLHLGITESGTLFAGTVKSAAGLGAILNKGIGNTLRISLSA
CCCEEHHHHHHHHHHHCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEEECC
DPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISTLQVPIKVAV
CCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHCCEEEEE
LGCAVNGPGEAREADIGIAGARGEGLLFRKGQVVRKVPEEIMVEELKKEIDVIAAEMAAE
EEEEECCCCCCCCCCCEEECCCCCCEEEECCHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
REKEKEKEKEKEKETQEQ
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNEMTHRTKTRPVKVGNLTIGGNNELIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVA
CCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEE
VPDERAANAIADIKKQINIPLVADIHFDYRLALKAIEGGIDKVRINPGNIGRRHKVEAVV
CCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHH
NAAKERGIPIRIGVNAGSLERHILEKYGYPTADGMVESALHHIKILEDLDFHDIIVSMKA
HHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEEC
SDVNLAIEAYEKAARAFDYPLHLGITESGTLFAGTVKSAAGLGAILNKGIGNTLRISLSA
CCCEEHHHHHHHHHHHCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEEECC
DPVEEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISTLQVPIKVAV
CCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHCCEEEEE
LGCAVNGPGEAREADIGIAGARGEGLLFRKGQVVRKVPEEIMVEELKKEIDVIAAEMAAE
EEEEECCCCCCCCCCCEEECCCCCCEEEECCHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
REKEKEKEKEKEKETQEQ
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA