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The map label for this gene is ispH

Identifier: 52141212

GI number: 52141212

Start: 4146647

End: 4147597

Strand: Direct

Name: ispH

Synonym: BCZK4038

Alternate gene names: 52141212

Gene position: 4146647-4147597 (Clockwise)

Preceding gene: 52141213

Following gene: 161763550

Centisome position: 78.23

GC content: 38.28

Gene sequence:

>951_bases
ATGAAAATTGTTAAAATTTCCCCTCGTGGTTATTGCTACGGTGTTGTGGATGCGATGGTTATTGCACGTAACGCCGCATT
AGATACATCATTACCAAGACCTATTTATATTTTAGGTATGATTGTTCACAACAAACATGTAACAGATGCATTCGAAGAAG
ATGGCATCATCACTTTAGACGGTCCAAGTCGATTAGACATTTTAGATAAAATCGATTCTGGTACTGTTATTTTCACTGCA
CACGGTGTTTCTCCAGAAGTTAAACAACGTGCAAAAGAAAAAGGTTTAACAACAATCGATGCCACTTGTCCAGATGTTAC
AAAAACACATGACCTTATTGAAGCAAAGAAAGCTGAAGGTTACCATGTCATTTATATCGGCAAAAAAAATCATCCAGAAC
CGGAAGGCGCAGTTGGTATCGCACCTGATATCGTTCACCTTATCGAAAGAGCCGATGATTTAAAAACATTAGAAATTCCA
ACGGATAAAATTTTAGTTACAAATCAAACAACGATGAGCCAATGGGATGTGCAACATTTAATGGAGGACATTCAGAAAAA
ATTCCCAACAGCAGAATTTCATAAGGAAATTTGTTTAGCAACTCAAGTTCGCCAAGAAGCTGTTGCCAAACAAGCTGATG
TTGCCGACTTAACAATTGTTGTTGGTGATCCGAAAAGTAACAACTCAAACCGTTTAGCACAAGTATCACAAGAAATCGCT
GGTACGAAGGCATACCGCGTTGCAGACGTAAGTGAGATTAAATTAGAATGGCTACAGGGTGTAGAAAACGTAGCTGTTAC
AGCAGGTGCTTCTACTCCAACACCAATTACAAAAGAGGTTATCGCTTTCTTAGAGCAATATGACCCAATGAATCCCGCTA
CATGGGAGAGAGTTCGAAAAGTACCGTTACAAAAAATATTACCTCGTGTAAAAGTGAAAAAAGAACAATAA

Upstream 100 bases:

>100_bases
TATATGTGTAACAATCCTTTGTTTTCTATCCACTCCTCCTTTATAATGTAAAAGACTATGCACAAAAGTATCCCTTGTTT
AGAAGGAGAGGATTACTCAT

Downstream 100 bases:

>100_bases
TAAAAACCGTTGCCTATATGAGCAACGGTTTTATTTTTCTTATACAAATGTAAATGGATCTGTATGTAACTGCGAAGCAT
GAATGTTTACATTAAGTTTC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKIVKISPRGYCYGVVDAMVIARNAALDTSLPRPIYILGMIVHNKHVTDAFEEDGIITLDGPSRLDILDKIDSGTVIFTA
HGVSPEVKQRAKEKGLTTIDATCPDVTKTHDLIEAKKAEGYHVIYIGKKNHPEPEGAVGIAPDIVHLIERADDLKTLEIP
TDKILVTNQTTMSQWDVQHLMEDIQKKFPTAEFHKEICLATQVRQEAVAKQADVADLTIVVGDPKSNNSNRLAQVSQEIA
GTKAYRVADVSEIKLEWLQGVENVAVTAGASTPTPITKEVIAFLEQYDPMNPATWERVRKVPLQKILPRVKVKKEQ

Sequences:

>Translated_316_residues
MKIVKISPRGYCYGVVDAMVIARNAALDTSLPRPIYILGMIVHNKHVTDAFEEDGIITLDGPSRLDILDKIDSGTVIFTA
HGVSPEVKQRAKEKGLTTIDATCPDVTKTHDLIEAKKAEGYHVIYIGKKNHPEPEGAVGIAPDIVHLIERADDLKTLEIP
TDKILVTNQTTMSQWDVQHLMEDIQKKFPTAEFHKEICLATQVRQEAVAKQADVADLTIVVGDPKSNNSNRLAQVSQEIA
GTKAYRVADVSEIKLEWLQGVENVAVTAGASTPTPITKEVIAFLEQYDPMNPATWERVRKVPLQKILPRVKVKKEQ
>Mature_316_residues
MKIVKISPRGYCYGVVDAMVIARNAALDTSLPRPIYILGMIVHNKHVTDAFEEDGIITLDGPSRLDILDKIDSGTVIFTA
HGVSPEVKQRAKEKGLTTIDATCPDVTKTHDLIEAKKAEGYHVIYIGKKNHPEPEGAVGIAPDIVHLIERADDLKTLEIP
TDKILVTNQTTMSQWDVQHLMEDIQKKFPTAEFHKEICLATQVRQEAVAKQADVADLTIVVGDPKSNNSNRLAQVSQEIA
GTKAYRVADVSEIKLEWLQGVENVAVTAGASTPTPITKEVIAFLEQYDPMNPATWERVRKVPLQKILPRVKVKKEQ

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=292, Percent_Identity=38.013698630137, Blast_Score=184, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_BACAA (C3P8J4)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868577.1
- EnsemblBacteria:   EBBACT00000128579
- GeneID:   7848900
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4532
- GeneTree:   EBGT00050000002965
- ProtClustDB:   PRK01045
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34968; Mature: 34968

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVKISPRGYCYGVVDAMVIARNAALDTSLPRPIYILGMIVHNKHVTDAFEEDGIITLD
CEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHHHCCCEEEEC
GPSRLDILDKIDSGTVIFTAHGVSPEVKQRAKEKGLTTIDATCPDVTKTHDLIEAKKAEG
CCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCC
YHVIYIGKKNHPEPEGAVGIAPDIVHLIERADDLKTLEIPTDKILVTNQTTMSQWDVQHL
EEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHHHH
MEDIQKKFPTAEFHKEICLATQVRQEAVAKQADVADLTIVVGDPKSNNSNRLAQVSQEIA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHH
GTKAYRVADVSEIKLEWLQGVENVAVTAGASTPTPITKEVIAFLEQYDPMNPATWERVRK
CCCEEEECCHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
VPLQKILPRVKVKKEQ
CCHHHHCCCCCCCCCC
>Mature Secondary Structure
MKIVKISPRGYCYGVVDAMVIARNAALDTSLPRPIYILGMIVHNKHVTDAFEEDGIITLD
CEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHHHCCCEEEEC
GPSRLDILDKIDSGTVIFTAHGVSPEVKQRAKEKGLTTIDATCPDVTKTHDLIEAKKAEG
CCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCC
YHVIYIGKKNHPEPEGAVGIAPDIVHLIERADDLKTLEIPTDKILVTNQTTMSQWDVQHL
EEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHHHH
MEDIQKKFPTAEFHKEICLATQVRQEAVAKQADVADLTIVVGDPKSNNSNRLAQVSQEIA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHH
GTKAYRVADVSEIKLEWLQGVENVAVTAGASTPTPITKEVIAFLEQYDPMNPATWERVRK
CCCEEEECCHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
VPLQKILPRVKVKKEQ
CCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA