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The map label for this gene is nadB [H]

Identifier: 52141075

GI number: 52141075

Start: 4281762

End: 4283291

Strand: Reverse

Name: nadB [H]

Synonym: BCZK4175

Alternate gene names: 52141075

Gene position: 4283291-4281762 (Counterclockwise)

Preceding gene: 52141071

Following gene: 52141076

Centisome position: 80.8

GC content: 39.28

Gene sequence:

>1530_bases
ATGCCAAGTGCAGATGTCTTAATTATTGGAAGTGGTATTGCGGCACTTCGTGTTGCAAAAGAGATTTGCCACGAAAAGAA
TGTGATTATTATCACAAAGGAAACGAATCGTAATAATAATACACATTTAGCGCAAGGTGGAATTGCCGCAGCGGTTGCTA
CATATGACAATCCGAATGATCATTTTGAAGATACGTTAGTAGCAGGATGTCATTATAACAATGAAGATGTGGTCCGTTAT
TTAGTTGAAGAAGGACCGAAAGAAATGAAGAATCTCATTGAAAATGGAATGCAATTTGATGGAGATGAAACGGGTCCCCA
TCTTGGAAAAGAGGGTGCACATCGAAAGCGTCGTATTTTACATGCAGGTGGCGATGCAACAGGAAAGAATTTATTAGAGC
ATTTCATTCAAGAAGTAGTTCCGCAAGTTACGGTAGTGGAACAGGAAATGGTGCTTGATTTTATTATAGAAAGCGAAAAA
TGTGTTGGAGTTTTAACGCGAGATAGTGAAGGGAAGTTGAAGCGTTATGATGCAGATTATACTGTGCTAGCCTCAGGCGG
GATTGGTGGTTTATACGCCTTTACTTCTAATGATAAAACAATTACGGGTGATGGACTTGCGATGGTATACCGCGCAGGCG
GCGAGCTCGTAGATTTAGAGTTTGTACAATTTCATCCAACAATGTTATATGCGGGCGGGCGATGCTGTGGCCTCGTTTCT
GAAGCTGTCCGTGGTGAAGGAGCTGTCCTTATAAATGGAAAAGGGCAGCGTTTTATGATGGATATACACGAGGATCATGA
TCTCGCACCGCGTGATGTGGTGGCAAGAGCGATTCATGAACAGCTTTTGTCAGGTGAAAAAGTGTATTTGAATATCGAGT
CTATTCAAAATTTCGAAGAACGTTTTCCAACTGTATCAGCATTGTGTAAAAAGAATGGTGTTGATATTACTAAAAAACGC
ATTCCAGTCGTACCCGGTGCTCATTTTCATATGGGTGGCGTGAAAACAAACATTGATGGGGAGACGTCCATTCCAAATTT
ATATGCAGTCGGTGAAGTAGCTTGTAACGGTGTTCATGGAGCAAATAGATTAGCGAGTAATTCGTTATTAGAAGGTCTTG
TGTTTGGAAAAAGAATAGGACAACATATTTTAACGAAAGCGAAACAAGACAGATTGAACGACTTCGCTGAGAAGGAAAAG
AAATTTATCGTTCTAAATCATTTGCCGACGAAAGAAGAAATACAAGAATGCATGATGAAATATGTTGGGATTGTACGAAC
AGAGCAAAGTTTATCTTATGCGAAGAGATGGCTTAGTAAATACGGTGTTCGAAATATGATATTACAACATGATGCTCTTA
CAAATGAAGAGATAACGCTTATTAATATGTTAACAGTTTGTGAGCTTATCGTCGTATCAGCTTTACAAAGAGAAGAAAGC
ATTGGTGGTCATTATCGAAGTGATTATCCACATAGAAATAGCGTGAAAAAAGAGATCATTCGAGTGAAAAGAAAACTACA
ACTTGTGTAA

Upstream 100 bases:

>100_bases
AGTGAAAATAAGAATTATAAAAAACTCTTGTCATTATTTTAAAACTATGTAAATATAGGTGTCAAGACAGTTGAAAAGTG
TAAAGTAGGAGGCAACAATT

Downstream 100 bases:

>100_bases
TGAGGGGAAGAGGGACTTTATGAATACGATAAAGGTTAAAGAAGCATTAAATCGATTTTTTCTAGAAGATATAGGAGAAA
GAGATGTAACATCTCAGCTT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MPSADVLIIGSGIAALRVAKEICHEKNVIIITKETNRNNNTHLAQGGIAAAVATYDNPNDHFEDTLVAGCHYNNEDVVRY
LVEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRILHAGGDATGKNLLEHFIQEVVPQVTVVEQEMVLDFIIESEK
CVGVLTRDSEGKLKRYDADYTVLASGGIGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGRCCGLVS
EAVRGEGAVLINGKGQRFMMDIHEDHDLAPRDVVARAIHEQLLSGEKVYLNIESIQNFEERFPTVSALCKKNGVDITKKR
IPVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHGANRLASNSLLEGLVFGKRIGQHILTKAKQDRLNDFAEKEK
KFIVLNHLPTKEEIQECMMKYVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREES
IGGHYRSDYPHRNSVKKEIIRVKRKLQLV

Sequences:

>Translated_509_residues
MPSADVLIIGSGIAALRVAKEICHEKNVIIITKETNRNNNTHLAQGGIAAAVATYDNPNDHFEDTLVAGCHYNNEDVVRY
LVEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRILHAGGDATGKNLLEHFIQEVVPQVTVVEQEMVLDFIIESEK
CVGVLTRDSEGKLKRYDADYTVLASGGIGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGRCCGLVS
EAVRGEGAVLINGKGQRFMMDIHEDHDLAPRDVVARAIHEQLLSGEKVYLNIESIQNFEERFPTVSALCKKNGVDITKKR
IPVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHGANRLASNSLLEGLVFGKRIGQHILTKAKQDRLNDFAEKEK
KFIVLNHLPTKEEIQECMMKYVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREES
IGGHYRSDYPHRNSVKKEIIRVKRKLQLV
>Mature_508_residues
PSADVLIIGSGIAALRVAKEICHEKNVIIITKETNRNNNTHLAQGGIAAAVATYDNPNDHFEDTLVAGCHYNNEDVVRYL
VEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRILHAGGDATGKNLLEHFIQEVVPQVTVVEQEMVLDFIIESEKC
VGVLTRDSEGKLKRYDADYTVLASGGIGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGRCCGLVSE
AVRGEGAVLINGKGQRFMMDIHEDHDLAPRDVVARAIHEQLLSGEKVYLNIESIQNFEERFPTVSALCKKNGVDITKKRI
PVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHGANRLASNSLLEGLVFGKRIGQHILTKAKQDRLNDFAEKEKK
FIVLNHLPTKEEIQECMMKYVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREESI
GGHYRSDYPHRNSVKKEIIRVKRKLQLV

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=524, Percent_Identity=33.206106870229, Blast_Score=216, Evalue=3e-56,
Organism=Escherichia coli, GI1788928, Length=521, Percent_Identity=36.6602687140115, Blast_Score=294, Evalue=1e-80,
Organism=Escherichia coli, GI1790597, Length=514, Percent_Identity=31.1284046692607, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1786942, Length=522, Percent_Identity=30.6513409961686, Blast_Score=189, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17505833, Length=524, Percent_Identity=32.0610687022901, Blast_Score=215, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17550100, Length=522, Percent_Identity=31.992337164751, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI71986328, Length=462, Percent_Identity=25.7575757575758, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322701, Length=522, Percent_Identity=34.4827586206897, Blast_Score=229, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6322416, Length=517, Percent_Identity=33.6557059961315, Blast_Score=221, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6320788, Length=485, Percent_Identity=26.3917525773196, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322511, Length=473, Percent_Identity=25.7928118393235, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17137288, Length=523, Percent_Identity=34.4168260038241, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24655642, Length=523, Percent_Identity=34.4168260038241, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24655647, Length=523, Percent_Identity=34.4168260038241, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24663005, Length=523, Percent_Identity=30.7839388145315, Blast_Score=200, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 56437; Mature: 56306

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00079 MULTICOPPER_OXIDASE1 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSADVLIIGSGIAALRVAKEICHEKNVIIITKETNRNNNTHLAQGGIAAAVATYDNPND
CCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCEEEEEEECCCCCC
HFEDTLVAGCHYNNEDVVRYLVEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRIL
CHHHHEEECCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHE
HAGGDATGKNLLEHFIQEVVPQVTVVEQEMVLDFIIESEKCVGVLTRDSEGKLKRYDADY
ECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCCE
TVLASGGIGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGRCCGLVS
EEEECCCCCEEEEEECCCCEECCCCEEEEEECCCCEEEEEEEEECCEEEECCCCHHHHHH
EAVRGEGAVLINGKGQRFMMDIHEDHDLAPRDVVARAIHEQLLSGEKVYLNIESIQNFEE
HHHCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHH
RFPTVSALCKKNGVDITKKRIPVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHG
HCCHHHHHHHCCCCCCHHHCCCCCCCCCEECCCEEECCCCCCCCCCEEEEHHHHHCCCCC
ANRLASNSLLEGLVFGKRIGQHILTKAKQDRLNDFAEKEKKFIVLNHLPTKEEIQECMMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
YVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREES
HHHHHCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGGHYRSDYPHRNSVKKEIIRVKRKLQLV
CCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSADVLIIGSGIAALRVAKEICHEKNVIIITKETNRNNNTHLAQGGIAAAVATYDNPND
CCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCEEEEEEECCCCCC
HFEDTLVAGCHYNNEDVVRYLVEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRIL
CHHHHEEECCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHE
HAGGDATGKNLLEHFIQEVVPQVTVVEQEMVLDFIIESEKCVGVLTRDSEGKLKRYDADY
ECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCCE
TVLASGGIGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGRCCGLVS
EEEECCCCCEEEEEECCCCEECCCCEEEEEECCCCEEEEEEEEECCEEEECCCCHHHHHH
EAVRGEGAVLINGKGQRFMMDIHEDHDLAPRDVVARAIHEQLLSGEKVYLNIESIQNFEE
HHHCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHH
RFPTVSALCKKNGVDITKKRIPVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHG
HCCHHHHHHHCCCCCCHHHCCCCCCCCCEECCCEEECCCCCCCCCCEEEEHHHHHCCCCC
ANRLASNSLLEGLVFGKRIGQHILTKAKQDRLNDFAEKEKKFIVLNHLPTKEEIQECMMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
YVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREES
HHHHHCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGGHYRSDYPHRNSVKKEIIRVKRKLQLV
CCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8444804; 9384377 [H]