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The map label for this gene is nifS [H]

Identifier: 52141074

GI number: 52141074

Start: 4283443

End: 4284585

Strand: Direct

Name: nifS [H]

Synonym: BCZK4176

Alternate gene names: 52141074

Gene position: 4283443-4284585 (Clockwise)

Preceding gene: 52141081

Following gene: 52141073

Centisome position: 80.81

GC content: 37.62

Gene sequence:

>1143_bases
ATGATGATATATCTTGACTATGCAGCTACTACACCTATGAGTGTGGAAGCATTACAAACATATACGAAAGCAGCATCGCA
ATACTTCGGAAACGAACAAAGTTTACACGATATTGGAGGAACAGCCTCTTCTTTATTACAAGTTTGCAGAAAAACATTTG
CTGACATGATCGGTGGAAAGGAACAAGGGGTATTCTTCACGAGCGGCGGGTCAGAATCGAACTATCTTGCGATTCAATCC
CTTCTCAATGCCCAACATAAGAAGCATATTATTACGACACCTATGGAACATGCATCCATTCGAAGTTATTTTCAATCTCT
AAAATCACAAGGCTATACGATTACTGAAATTCCTGTTGATAAAAGTGGACTCATTCGTTTAGTAGATTTAGAAACAGCTA
TTACAGAAGATACCGCTCTAGCAAGTATACAGCACGGAAACTCTGAAATTGGAACTGTTCAAAATATCGGAGAGATCGGG
GCACTTTTAAAAAAATATAACGTTTTATTTCATTCAGATTGTGTGCAAACATTTGGTAAACTTCCTATCCATGTTTTTGA
GATGGGGATTGATAGTCTTTCTGTTTCAGCACATAAAATATACGGACCAAAGGGTGTTGGCGCTTGCTATATAAATCCGC
AAGTTCGCTGGAAACAAGTATTTCCGGAAACTTCTCACGAAAAAGGTTTTCGCCCTGGCACAGTAAACGTCCCTGGCATC
GCATCTTTTTTAACAGCTGCTGAGAATATATTAAAAAATCAGCAAGAAGAAAGTTTACGTTTTAAAGCATTACGATCCTA
CTTTTTAGAGCAAATACAAACACTTCCGCTAGAAATTGAAGTAGAAGGTCATTCTACTTCTTGTTTACCACATATTATTG
GGGTTACAATAAAAGGAATAGAAGGTCAGTATACAATGCTAGAATGTAATCGCCGCGGTATTGCTATTTCCACGGGAAGT
GCTTGCCAAGTCGGTAAACAAGAACCTTCGAAAACAATGCTTGCAATTGGAAAAACGTATGAAGAGGCAAAACAATATGT
CCGCTTCTCTTTGGGACAACAAACAACGAAAGATCAAATTGATACTACTATTCATGCACTACACACAATTGGAAATCAAT
TTTATAGAGGTGTTAAATCATAA

Upstream 100 bases:

>100_bases
CATAGTTTTAAAATAATGACAAGAGTTTTTTATAATTCTTATTTTCACTACCATTTAAAAAATAAAAGAAATGTAACTCT
TACTAGAGAGAGGAACTTAC

Downstream 100 bases:

>100_bases
TGAAACAAAATGAACAAAAAAAGATTTTAGGTGAAGAAAGACGACAGCTTATCCTCCAGTGGCTTCTTGCTGCAAATGAA
CCGTTATCGGGGAATGAACT

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MMIYLDYAATTPMSVEALQTYTKAASQYFGNEQSLHDIGGTASSLLQVCRKTFADMIGGKEQGVFFTSGGSESNYLAIQS
LLNAQHKKHIITTPMEHASIRSYFQSLKSQGYTITEIPVDKSGLIRLVDLETAITEDTALASIQHGNSEIGTVQNIGEIG
ALLKKYNVLFHSDCVQTFGKLPIHVFEMGIDSLSVSAHKIYGPKGVGACYINPQVRWKQVFPETSHEKGFRPGTVNVPGI
ASFLTAAENILKNQQEESLRFKALRSYFLEQIQTLPLEIEVEGHSTSCLPHIIGVTIKGIEGQYTMLECNRRGIAISTGS
ACQVGKQEPSKTMLAIGKTYEEAKQYVRFSLGQQTTKDQIDTTIHALHTIGNQFYRGVKS

Sequences:

>Translated_380_residues
MMIYLDYAATTPMSVEALQTYTKAASQYFGNEQSLHDIGGTASSLLQVCRKTFADMIGGKEQGVFFTSGGSESNYLAIQS
LLNAQHKKHIITTPMEHASIRSYFQSLKSQGYTITEIPVDKSGLIRLVDLETAITEDTALASIQHGNSEIGTVQNIGEIG
ALLKKYNVLFHSDCVQTFGKLPIHVFEMGIDSLSVSAHKIYGPKGVGACYINPQVRWKQVFPETSHEKGFRPGTVNVPGI
ASFLTAAENILKNQQEESLRFKALRSYFLEQIQTLPLEIEVEGHSTSCLPHIIGVTIKGIEGQYTMLECNRRGIAISTGS
ACQVGKQEPSKTMLAIGKTYEEAKQYVRFSLGQQTTKDQIDTTIHALHTIGNQFYRGVKS
>Mature_380_residues
MMIYLDYAATTPMSVEALQTYTKAASQYFGNEQSLHDIGGTASSLLQVCRKTFADMIGGKEQGVFFTSGGSESNYLAIQS
LLNAQHKKHIITTPMEHASIRSYFQSLKSQGYTITEIPVDKSGLIRLVDLETAITEDTALASIQHGNSEIGTVQNIGEIG
ALLKKYNVLFHSDCVQTFGKLPIHVFEMGIDSLSVSAHKIYGPKGVGACYINPQVRWKQVFPETSHEKGFRPGTVNVPGI
ASFLTAAENILKNQQEESLRFKALRSYFLEQIQTLPLEIEVEGHSTSCLPHIIGVTIKGIEGQYTMLECNRRGIAISTGS
ACQVGKQEPSKTMLAIGKTYEEAKQYVRFSLGQQTTKDQIDTTIHALHTIGNQFYRGVKS

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine. Seems to be required for NAD biosynthesis [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=368, Percent_Identity=35.5978260869565, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI156713448, Length=406, Percent_Identity=31.5270935960591, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI48994898, Length=369, Percent_Identity=35.7723577235772, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1787970, Length=399, Percent_Identity=23.0576441102757, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25143064, Length=368, Percent_Identity=35.054347826087, Blast_Score=209, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17533177, Length=329, Percent_Identity=30.0911854103343, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319831, Length=370, Percent_Identity=35.4054054054054, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI20129463, Length=366, Percent_Identity=34.4262295081967, Blast_Score=219, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41827; Mature: 41827

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIYLDYAATTPMSVEALQTYTKAASQYFGNEQSLHDIGGTASSLLQVCRKTFADMIGGK
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCC
EQGVFFTSGGSESNYLAIQSLLNAQHKKHIITTPMEHASIRSYFQSLKSQGYTITEIPVD
CCCEEEECCCCCCCHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHCCCEEEECCCC
KSGLIRLVDLETAITEDTALASIQHGNSEIGTVQNIGEIGALLKKYNVLFHSDCVQTFGK
CCCCEEEEECHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPIHVFEMGIDSLSVSAHKIYGPKGVGACYINPQVRWKQVFPETSHEKGFRPGTVNVPGI
CCEEEEECCCCHHCCCHHEEECCCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCHH
ASFLTAAENILKNQQEESLRFKALRSYFLEQIQTLPLEIEVEGHSTSCLPHIIGVTIKGI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCEEEECC
EGQYTMLECNRRGIAISTGSACQVGKQEPSKTMLAIGKTYEEAKQYVRFSLGQQTTKDQI
CCCEEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCHHHHH
DTTIHALHTIGNQFYRGVKS
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMIYLDYAATTPMSVEALQTYTKAASQYFGNEQSLHDIGGTASSLLQVCRKTFADMIGGK
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCC
EQGVFFTSGGSESNYLAIQSLLNAQHKKHIITTPMEHASIRSYFQSLKSQGYTITEIPVD
CCCEEEECCCCCCCHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHCCCEEEECCCC
KSGLIRLVDLETAITEDTALASIQHGNSEIGTVQNIGEIGALLKKYNVLFHSDCVQTFGK
CCCCEEEEECHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPIHVFEMGIDSLSVSAHKIYGPKGVGACYINPQVRWKQVFPETSHEKGFRPGTVNVPGI
CCEEEEECCCCHHCCCHHEEECCCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCHH
ASFLTAAENILKNQQEESLRFKALRSYFLEQIQTLPLEIEVEGHSTSCLPHIIGVTIKGI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCEEEECC
EGQYTMLECNRRGIAISTGSACQVGKQEPSKTMLAIGKTYEEAKQYVRFSLGQQTTKDQI
CCCEEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCHHHHH
DTTIHALHTIGNQFYRGVKS
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8444804; 9384377 [H]