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The map label for this gene is spoU [H]

Identifier: 52140943

GI number: 52140943

Start: 4418965

End: 4419729

Strand: Reverse

Name: spoU [H]

Synonym: BCZK4305

Alternate gene names: 52140943

Gene position: 4419729-4418965 (Counterclockwise)

Preceding gene: 52140940

Following gene: 52140946

Centisome position: 83.38

GC content: 39.22

Gene sequence:

>765_bases
ATGAAAAACATTGATTCAGTACAAAATCCGCGTGTGAAGCAGTGGAAAAAGCTGCAGACGAAAAAAGAACGCGATAAAAA
AGGTTTATTTTTTGTAGAAGGATTCCATTTAGTAGAGGAAGCCTTAAAGGCAGGAGTTGTAACAGAACTTATCGTTTCAG
ATCAGACAGACCTTCCGAAAGATTGGACAGTTAATGATGTAGAAATGTATATCGTACCGGAAGCAATCGTAAAAGTACTT
CGTGAAACAGAAACGACGCAAGGTGTATTTGCTGTTTGTGAAAAGCATGAGAAGGAAGTAGCTCTTGCTGATGGGAAGTT
TCTTTTATTAGATGGCCTGCAAGACCCAGGTAACTTAGGGACAATTATTCGTACAGCTGATGCAGCTGGTATTCATGCCG
TTGTGCTTGGAGAAGGTTGTGTTGATGCATATAATAGTAAAGTACTCCGCTCTACACAAGGTTCTATTTTCCACTTACCA
ATTGTAAAAGGAAACTTAGAAGAATGGGTAGACAAACTAAAAGAAAATAACGTTCCTGTATATGGAACAGCTCTTGAAAA
TGGAGTTCCTTACGGTGAAGTAACACCAGCAGGAAGTTTTGCATTAATTGTAGGAAATGAAGGAAGTGGCGTACGCCAAG
AAGTTCTTGCAAAAACGGATCAAAACTTGTATATTCCGATTTACGGCGGGGCAGAATCATTAAATGTTGCGGTTGCAGCA
GGGATTTTAACGTATTATTTACAAAGCCCAGTTGCGAACAAATAA

Upstream 100 bases:

>100_bases
TTCAGAATTGAAACTGACGTTAGTTTTTGTTCTTTTTTATGTAAATATGTATGAAAAATAGGCTATAATGATAAGACAAG
AAGAGAGGGAAATACATATT

Downstream 100 bases:

>100_bases
AAAACTTCTTATAATAAGTTGAAGTACATTATATTAAAAGTTAAATAACGAAAAACGTTGATAGAGAAGAGTAGCTTACA
AAATCGCCATATAGGGAGAG

Product: 23S rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKNIDSVQNPRVKQWKKLQTKKERDKKGLFFVEGFHLVEEALKAGVVTELIVSDQTDLPKDWTVNDVEMYIVPEAIVKVL
RETETTQGVFAVCEKHEKEVALADGKFLLLDGLQDPGNLGTIIRTADAAGIHAVVLGEGCVDAYNSKVLRSTQGSIFHLP
IVKGNLEEWVDKLKENNVPVYGTALENGVPYGEVTPAGSFALIVGNEGSGVRQEVLAKTDQNLYIPIYGGAESLNVAVAA
GILTYYLQSPVANK

Sequences:

>Translated_254_residues
MKNIDSVQNPRVKQWKKLQTKKERDKKGLFFVEGFHLVEEALKAGVVTELIVSDQTDLPKDWTVNDVEMYIVPEAIVKVL
RETETTQGVFAVCEKHEKEVALADGKFLLLDGLQDPGNLGTIIRTADAAGIHAVVLGEGCVDAYNSKVLRSTQGSIFHLP
IVKGNLEEWVDKLKENNVPVYGTALENGVPYGEVTPAGSFALIVGNEGSGVRQEVLAKTDQNLYIPIYGGAESLNVAVAA
GILTYYLQSPVANK
>Mature_254_residues
MKNIDSVQNPRVKQWKKLQTKKERDKKGLFFVEGFHLVEEALKAGVVTELIVSDQTDLPKDWTVNDVEMYIVPEAIVKVL
RETETTQGVFAVCEKHEKEVALADGKFLLLDGLQDPGNLGTIIRTADAAGIHAVVLGEGCVDAYNSKVLRSTQGSIFHLP
IVKGNLEEWVDKLKENNVPVYGTALENGVPYGEVTPAGSFALIVGNEGSGVRQEVLAKTDQNLYIPIYGGAESLNVAVAA
GILTYYLQSPVANK

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=292, Percent_Identity=28.7671232876712, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1790623, Length=148, Percent_Identity=36.4864864864865, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790083, Length=149, Percent_Identity=29.5302013422819, Blast_Score=65, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24666840, Length=278, Percent_Identity=28.0575539568345, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27680; Mature: 27680

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIDSVQNPRVKQWKKLQTKKERDKKGLFFVEGFHLVEEALKAGVVTELIVSDQTDLPK
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCHHHHHHHCCCCCCCC
DWTVNDVEMYIVPEAIVKVLRETETTQGVFAVCEKHEKEVALADGKFLLLDGLQDPGNLG
CCCCCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCCC
TIIRTADAAGIHAVVLGEGCVDAYNSKVLRSTQGSIFHLPIVKGNLEEWVDKLKENNVPV
EEEEECCCCCEEEEEECCCHHHHHCCHHHHCCCCCEEEEEEEECCHHHHHHHHHHCCCCE
YGTALENGVPYGEVTPAGSFALIVGNEGSGVRQEVLAKTDQNLYIPIYGGAESLNVAVAA
EEEHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHH
GILTYYLQSPVANK
HHHHHHHHCCCCCC
>Mature Secondary Structure
MKNIDSVQNPRVKQWKKLQTKKERDKKGLFFVEGFHLVEEALKAGVVTELIVSDQTDLPK
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCHHHHHHHCCCCCCCC
DWTVNDVEMYIVPEAIVKVLRETETTQGVFAVCEKHEKEVALADGKFLLLDGLQDPGNLG
CCCCCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCCC
TIIRTADAAGIHAVVLGEGCVDAYNSKVLRSTQGSIFHLPIVKGNLEEWVDKLKENNVPV
EEEEECCCCCEEEEEECCCHHHHHCCHHHHCCCCCEEEEEEEECCHHHHHHHHHHCCCCE
YGTALENGVPYGEVTPAGSFALIVGNEGSGVRQEVLAKTDQNLYIPIYGGAESLNVAVAA
EEEHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHH
GILTYYLQSPVANK
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]