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The map label for this gene is mdh [H]

Identifier: 52140908

GI number: 52140908

Start: 4460171

End: 4461379

Strand: Reverse

Name: mdh [H]

Synonym: BCZK4344

Alternate gene names: 52140908

Gene position: 4461379-4460171 (Counterclockwise)

Preceding gene: 52140905

Following gene: 52140907

Centisome position: 84.16

GC content: 40.61

Gene sequence:

>1209_bases
ATGCATAAAGTGCATCAAGGAAAATTAGAAACTGTATCAAAAGTAAAAGTAGAAAATGCAAAAGATTTAAGTCTTGCATA
TTCTCCAGGGGTTGCAGAACCTTGTAAGGAAATTTATGACGATAAAAGTAAGGTATATGAATATACGATGAAGGGAAATA
TGGTAGCAGTTGTGACAGATGGAACGGCTGTACTTGGTCTTGGTAACATTGGACCTGAAGCATCTCTTCCAGTAATGGAA
GGTAAAGCTGTATTATTCAAGAGCTTTGCTGGTGTAGATGCATTCCCAATTGCCTTAAATACAAACGATGTAGATAAAAT
TGTTGAAACTGTAAAATTAATGGAGCCAACTTTTGGCGGCGTTAACTTAGAAGATATCGCAGCACCAAACTGCTTCATTA
TTGAAGAACGTTTGAAAAAAGAAACAAACATTCCTATCTTCCATGATGATCAACACGGAACAGCTATCGTAACAGTAGCA
GGTCTTGTGAACGCGCTAAAACTAGTTGGAAAGAAAATGTCTGACATTAAAGTTGTCGCAAATGGCGCAGGTGCAGCAGG
TATTGCAATTATTAAACTTTTATATCGCTATGGCGTACGCGACATTATTATGTGTGACCGTAAAGGCGCGATCTATGAAG
GTCGTCCTACAGGTATGAATCCGGTGAAGGATGAAGTTGCAAAATATACAAATAAGAATCGTATCGAAGGTTCTTTAGCT
GATGTTGTACAAGGTGCGGACGTATTCATTGGTGTATCTGCAGAAGGTGCATTAACAGAAGAGATGGTTCGTACAATGAA
TGACGATGCAATTATTTTTGCAATGGCGAATCCAGTTCCAGAAATTATGCCAGAATTGGCAAAAGCAGCGGGCGCAGCTG
TTGTTGGAACAGGTCGTTCTGACTTTGCAAACCAAGTAAATAATGTACTAGCGTTCCCTGGTATTTTCCGCGGTGCACTT
GACGTACATGCGACACAAATTAACGAAGAAATGAAGATGGCAGCTGTACAAGCCATTGCTGAGCTTGTAGCAGAAGATGA
GTTGAATGCAGACTATATCATTCCAGCACCGTTTGATGCGCGTGTAGCGCCTCAAGTAGCAGCTTACGTTGCGAAAGCTG
CAATGGAGACAGGAGTAGCTCGCCGTCAAGTGGATCCAAATGAAGTTGCTGAGAAAACAAAACAATTAGCGCTGATTGGT
AAAGAATAA

Upstream 100 bases:

>100_bases
AGTAGTGTTCGTGGTCAGACCACTTGGAGAAATTGATATCAGCAAGAATAATTTGAGGAGAGTGGATAGTTTGTCAACAC
TTCGTGAAGAAGCACTTCAC

Downstream 100 bases:

>100_bases
GCGAGGAATTTCCATTGACATCATCAAATACAAAAGTATATCTCGAAATTGTAAAAAAAATCCGTTCCATTATGGAAGAG
GATGGATTGGTAGCAGGGGA

Product: malate dehydrogenase (malic enzyme, NAD-malic enzyme)

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MHKVHQGKLETVSKVKVENAKDLSLAYSPGVAEPCKEIYDDKSKVYEYTMKGNMVAVVTDGTAVLGLGNIGPEASLPVME
GKAVLFKSFAGVDAFPIALNTNDVDKIVETVKLMEPTFGGVNLEDIAAPNCFIIEERLKKETNIPIFHDDQHGTAIVTVA
GLVNALKLVGKKMSDIKVVANGAGAAGIAIIKLLYRYGVRDIIMCDRKGAIYEGRPTGMNPVKDEVAKYTNKNRIEGSLA
DVVQGADVFIGVSAEGALTEEMVRTMNDDAIIFAMANPVPEIMPELAKAAGAAVVGTGRSDFANQVNNVLAFPGIFRGAL
DVHATQINEEMKMAAVQAIAELVAEDELNADYIIPAPFDARVAPQVAAYVAKAAMETGVARRQVDPNEVAEKTKQLALIG
KE

Sequences:

>Translated_402_residues
MHKVHQGKLETVSKVKVENAKDLSLAYSPGVAEPCKEIYDDKSKVYEYTMKGNMVAVVTDGTAVLGLGNIGPEASLPVME
GKAVLFKSFAGVDAFPIALNTNDVDKIVETVKLMEPTFGGVNLEDIAAPNCFIIEERLKKETNIPIFHDDQHGTAIVTVA
GLVNALKLVGKKMSDIKVVANGAGAAGIAIIKLLYRYGVRDIIMCDRKGAIYEGRPTGMNPVKDEVAKYTNKNRIEGSLA
DVVQGADVFIGVSAEGALTEEMVRTMNDDAIIFAMANPVPEIMPELAKAAGAAVVGTGRSDFANQVNNVLAFPGIFRGAL
DVHATQINEEMKMAAVQAIAELVAEDELNADYIIPAPFDARVAPQVAAYVAKAAMETGVARRQVDPNEVAEKTKQLALIG
KE
>Mature_402_residues
MHKVHQGKLETVSKVKVENAKDLSLAYSPGVAEPCKEIYDDKSKVYEYTMKGNMVAVVTDGTAVLGLGNIGPEASLPVME
GKAVLFKSFAGVDAFPIALNTNDVDKIVETVKLMEPTFGGVNLEDIAAPNCFIIEERLKKETNIPIFHDDQHGTAIVTVA
GLVNALKLVGKKMSDIKVVANGAGAAGIAIIKLLYRYGVRDIIMCDRKGAIYEGRPTGMNPVKDEVAKYTNKNRIEGSLA
DVVQGADVFIGVSAEGALTEEMVRTMNDDAIIFAMANPVPEIMPELAKAAGAAVVGTGRSDFANQVNNVLAFPGIFRGAL
DVHATQINEEMKMAAVQAIAELVAEDELNADYIIPAPFDARVAPQVAAYVAKAAMETGVARRQVDPNEVAEKTKQLALIG
KE

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=394, Percent_Identity=25.3807106598985, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI62420882, Length=395, Percent_Identity=27.8481012658228, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI62420880, Length=395, Percent_Identity=27.8481012658228, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI239049447, Length=395, Percent_Identity=27.8481012658228, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI4505145, Length=405, Percent_Identity=24.9382716049383, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788806, Length=395, Percent_Identity=52.4050632911392, Blast_Score=401, Evalue=1e-113,
Organism=Escherichia coli, GI87081919, Length=458, Percent_Identity=28.82096069869, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17537199, Length=452, Percent_Identity=27.4336283185841, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322823, Length=311, Percent_Identity=30.5466237942122, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI78707242, Length=407, Percent_Identity=27.5184275184275, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI78707238, Length=407, Percent_Identity=27.5184275184275, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21356279, Length=452, Percent_Identity=27.4336283185841, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI281362672, Length=452, Percent_Identity=27.4336283185841, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI281362674, Length=452, Percent_Identity=27.4336283185841, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24646386, Length=451, Percent_Identity=25.9423503325942, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24646388, Length=400, Percent_Identity=27.25, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI78707240, Length=400, Percent_Identity=27, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 42916; Mature: 42916

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKVHQGKLETVSKVKVENAKDLSLAYSPGVAEPCKEIYDDKSKVYEYTMKGNMVAVVTD
CCCCCCCCHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHCCHHHHEEEEECCCEEEEEEC
GTAVLGLGNIGPEASLPVMEGKAVLFKSFAGVDAFPIALNTNDVDKIVETVKLMEPTFGG
CEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCC
VNLEDIAAPNCFIIEERLKKETNIPIFHDDQHGTAIVTVAGLVNALKLVGKKMSDIKVVA
CCHHHHCCCCEEEEHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHHHCCCCCCEEEEE
NGAGAAGIAIIKLLYRYGVRDIIMCDRKGAIYEGRPTGMNPVKDEVAKYTNKNRIEGSLA
CCCCHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCCCCCHHHHHHHHHCCCCCCCCHHH
DVVQGADVFIGVSAEGALTEEMVRTMNDDAIIFAMANPVPEIMPELAKAAGAAVVGTGRS
HHHCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEECCCH
DFANQVNNVLAFPGIFRGALDVHATQINEEMKMAAVQAIAELVAEDELNADYIIPAPFDA
HHHHHHHHHECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCC
RVAPQVAAYVAKAAMETGVARRQVDPNEVAEKTKQLALIGKE
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCC
>Mature Secondary Structure
MHKVHQGKLETVSKVKVENAKDLSLAYSPGVAEPCKEIYDDKSKVYEYTMKGNMVAVVTD
CCCCCCCCHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHCCHHHHEEEEECCCEEEEEEC
GTAVLGLGNIGPEASLPVMEGKAVLFKSFAGVDAFPIALNTNDVDKIVETVKLMEPTFGG
CEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCC
VNLEDIAAPNCFIIEERLKKETNIPIFHDDQHGTAIVTVAGLVNALKLVGKKMSDIKVVA
CCHHHHCCCCEEEEHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHHHCCCCCCEEEEE
NGAGAAGIAIIKLLYRYGVRDIIMCDRKGAIYEGRPTGMNPVKDEVAKYTNKNRIEGSLA
CCCCHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCCCCCHHHHHHHHHCCCCCCCCHHH
DVVQGADVFIGVSAEGALTEEMVRTMNDDAIIFAMANPVPEIMPELAKAAGAAVVGTGRS
HHHCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEECCCH
DFANQVNNVLAFPGIFRGALDVHATQINEEMKMAAVQAIAELVAEDELNADYIIPAPFDA
HHHHHHHHHECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCC
RVAPQVAAYVAKAAMETGVARRQVDPNEVAEKTKQLALIGKE
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]