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The map label for this gene is bsdA [H]

Identifier: 52140654

GI number: 52140654

Start: 4687408

End: 4688301

Strand: Reverse

Name: bsdA [H]

Synonym: BCZK4600

Alternate gene names: 52140654

Gene position: 4688301-4687408 (Counterclockwise)

Preceding gene: 52140652

Following gene: 52140655

Centisome position: 88.44

GC content: 35.35

Gene sequence:

>894_bases
ATGGATATGAGACAACTACGATATTTCATAGCAATTGTAGAGGAAAGAAAAATTTCAGCTGCTGCGGAAAGACTTCATAT
TTCTCAACCACCGCTAAGTCAGCACCTAAAAGCGATGGAGGATGAATTAGGAACGAAACTAATGGAGAGAAATGGGAAGT
TTTTAGGAGTAACGGAAGCAGGGAAAGCTTTATATAAGTATGCGCTACAAATGAATCATCTAATGGAAGAAGCAAAAATG
GAGGTAAAGGAAGTTGGAAACGGGGTAAGTGGAAAACTGACGATAGGCATCAATACTTTTTCGGTATCCGGATTACCTGA
TTTACTTCACAGGTTTCATAAAGAATATCCTAGAGTTACATATAAAGTTCATCAAAATGAGTCATCTCATCTTTGTCAAT
TAGTGAGAGAAAGGGTTATAGAGATGGCGATTATCCGACTACCGCTTGATTTAAATGATTTTTCAGCGATCCATCTTTAT
ACAGAACCTTTTTACTTTATTACTTCAAATGAAAAGCAACTTTTTAATCATCAGGTTACGCTTGCTGACATTCAAAATCA
TCCTTTGATATTACCAAGTACAGAAGGATTGGGAGTTCATTATATGATTTTGGAAGCATTTTCGAAAGCTCAACTACATC
CGAATATTATTGCGGAGTGCTCTGACATCTCTCTTTTACTAGATTTAGTCGCTTGTAACTTTGGAGCAGCTATTGTTCCA
GAAACTGTAATTAACCAATATCAAAACTACAATATTCAGGCTTTCAAAATTACAGATACAGATTTAATTGCATCTACGAG
CTTAATATGGCTGAAAAATCATTATCTATCAAAAGCCGCTCAAAACTTTATAGAACTGTTAACGGAGGATATCCAGAAAC
AAACAGATTTATAG

Upstream 100 bases:

>100_bases
TTTCAGCTAAAATTATGATATCTTAGAATGTAGTATATGAAAAATATAAAAAAATTTAATTTTACTTAAGTTTTTCATAT
AATGAGGAGGTTTTTTACTG

Downstream 100 bases:

>100_bases
CGTTAAGGATATTCCAACGTTCAAAATCCCAATCACTCATTGCTCTAATGGAGAAATTGGGATTTGTTTTTTTAACTATA
TGGCTCTTTAATGAATCGAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Bsd operon regulatory protein [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MDMRQLRYFIAIVEERKISAAAERLHISQPPLSQHLKAMEDELGTKLMERNGKFLGVTEAGKALYKYALQMNHLMEEAKM
EVKEVGNGVSGKLTIGINTFSVSGLPDLLHRFHKEYPRVTYKVHQNESSHLCQLVRERVIEMAIIRLPLDLNDFSAIHLY
TEPFYFITSNEKQLFNHQVTLADIQNHPLILPSTEGLGVHYMILEAFSKAQLHPNIIAECSDISLLLDLVACNFGAAIVP
ETVINQYQNYNIQAFKITDTDLIASTSLIWLKNHYLSKAAQNFIELLTEDIQKQTDL

Sequences:

>Translated_297_residues
MDMRQLRYFIAIVEERKISAAAERLHISQPPLSQHLKAMEDELGTKLMERNGKFLGVTEAGKALYKYALQMNHLMEEAKM
EVKEVGNGVSGKLTIGINTFSVSGLPDLLHRFHKEYPRVTYKVHQNESSHLCQLVRERVIEMAIIRLPLDLNDFSAIHLY
TEPFYFITSNEKQLFNHQVTLADIQNHPLILPSTEGLGVHYMILEAFSKAQLHPNIIAECSDISLLLDLVACNFGAAIVP
ETVINQYQNYNIQAFKITDTDLIASTSLIWLKNHYLSKAAQNFIELLTEDIQKQTDL
>Mature_297_residues
MDMRQLRYFIAIVEERKISAAAERLHISQPPLSQHLKAMEDELGTKLMERNGKFLGVTEAGKALYKYALQMNHLMEEAKM
EVKEVGNGVSGKLTIGINTFSVSGLPDLLHRFHKEYPRVTYKVHQNESSHLCQLVRERVIEMAIIRLPLDLNDFSAIHLY
TEPFYFITSNEKQLFNHQVTLADIQNHPLILPSTEGLGVHYMILEAFSKAQLHPNIIAECSDISLLLDLVACNFGAAIVP
ETVINQYQNYNIQAFKITDTDLIASTSLIWLKNHYLSKAAQNFIELLTEDIQKQTDL

Specific function: Could be a positive regulator of bsdBCD expression in response to salicylic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=291, Percent_Identity=29.2096219931272, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI157672245, Length=288, Percent_Identity=26.3888888888889, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788297, Length=244, Percent_Identity=30.327868852459, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1788748, Length=289, Percent_Identity=22.1453287197232, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787530, Length=308, Percent_Identity=24.3506493506494, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788887, Length=298, Percent_Identity=23.1543624161074, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI2367136, Length=175, Percent_Identity=28.5714285714286, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1790399, Length=193, Percent_Identity=27.979274611399, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787806, Length=289, Percent_Identity=23.5294117647059, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790208, Length=249, Percent_Identity=26.5060240963855, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789506, Length=295, Percent_Identity=21.3559322033898, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787601, Length=141, Percent_Identity=24.822695035461, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788296, Length=308, Percent_Identity=25.6493506493506, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI145693105, Length=288, Percent_Identity=21.1805555555556, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789639, Length=116, Percent_Identity=31.8965517241379, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788481, Length=291, Percent_Identity=23.3676975945017, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33839; Mature: 33839

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMRQLRYFIAIVEERKISAAAERLHISQPPLSQHLKAMEDELGTKLMERNGKFLGVTEA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
GKALYKYALQMNHLMEEAKMEVKEVGNGVSGKLTIGINTFSVSGLPDLLHRFHKEYPRVT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCEE
YKVHQNESSHLCQLVRERVIEMAIIRLPLDLNDFSAIHLYTEPFYFITSNEKQLFNHQVT
EEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEEECCCHHHHHCCEE
LADIQNHPLILPSTEGLGVHYMILEAFSKAQLHPNIIAECSDISLLLDLVACNFGAAIVP
EEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHCC
ETVINQYQNYNIQAFKITDTDLIASTSLIWLKNHYLSKAAQNFIELLTEDIQKQTDL
HHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDMRQLRYFIAIVEERKISAAAERLHISQPPLSQHLKAMEDELGTKLMERNGKFLGVTEA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
GKALYKYALQMNHLMEEAKMEVKEVGNGVSGKLTIGINTFSVSGLPDLLHRFHKEYPRVT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCEE
YKVHQNESSHLCQLVRERVIEMAIIRLPLDLNDFSAIHLYTEPFYFITSNEKQLFNHQVT
EEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEEECCCHHHHHCCEE
LADIQNHPLILPSTEGLGVHYMILEAFSKAQLHPNIIAECSDISLLLDLVACNFGAAIVP
EEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHCC
ETVINQYQNYNIQAFKITDTDLIASTSLIWLKNHYLSKAAQNFIELLTEDIQKQTDL
HHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]