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The map label for this gene is glnT [H]

Identifier: 52140482

GI number: 52140482

Start: 4868423

End: 4869856

Strand: Direct

Name: glnT [H]

Synonym: BCZK4777

Alternate gene names: 52140482

Gene position: 4868423-4869856 (Clockwise)

Preceding gene: 52140487

Following gene: 52140480

Centisome position: 91.84

GC content: 38.42

Gene sequence:

>1434_bases
ATGGTTGATATCTTTAGCAGATTCCTTGAGGTAACGAATAATATTTTATGGTCATATATTCTTATTGCAATGCTAATCGG
CTTCGGTCTTTATTTCTCTTTTAAATTGAAGTTTGTCCAAATTACTCATTTCGGTGAGATGGTCGGTTTAATTAGTAAAG
GATTTAATCGAAAAGAAAAAAAGAAAGATAGCATCTCCCCGTTCCAAGCATTTTGCTTAAGTGCAGCTGCACGTATCGGT
ATCGGAAACTTAGCTGGTGTAGCTTTAGCTATTTCAATGGGCGGACCCGGCGCAGTATTTTGGATGTGGTTTATCGCTAT
CCTTGGAGCAGCTACTAGTTTCGTAGAATGTACGCTCGCGCAAATTTATAAAGTAAAAGATGGAAGCAGATTCCGTGGTG
GCCCAGCATATTATATGGAAAAAGGATTAAACAAACGCTGGATGGGTATTTGGTTCTCACTTCTTATTACAGTTGCGTAC
GGATTAATTTTCAACTCTGTACAAGCAAACACAGTAACAATAGCATTTGAAAATGCTTTTGGACTAGAGCGAACGATCGT
AGGAGCTCTATTAGCTTTATTAGTTGCAGTTATTATTTTCGGTGGTATCAAGAGCATTTCACGTATTACGGAAATGATTG
TTCCTCCAATGGCAATCATTTATATTGGTGTGGCTATTTTTGTTGTTATTAACAACTTTACTATGTTACCAAGCATTTTT
ATGGAAATATTCAATGGTGCATTCGGTTTAGACCAAGCCATCGCTGGTGGTATTGGAGCAGCAATCAAGTTCGGAATTCA
GCGCGGTTTATTCGCGACAGAAGCAGGTATGGGTAGCTCTCCTAACGCAGCCGCAACATCAGATGTATCTCACCCTGTAA
AACAAGGACTTGTTCAAGCATTAGGTGTTTTCGTAGACACATTCTTAGTATGTACATCAACAGCATTTATCGTATTATGC
TCTGGACTTTACAAAGGAACAAATTTACAAGGTATTGAATTAACACAAAGTGCATTAAGTTCACAAATCGGACCATGGGC
AAGCACTTTCTTAGCGATTATTATTTTCCTATTCGCGTTCAGTTCTTTACTAGGAAACTACTATTATGGTGAAACAAACA
TCGCATTCATTAAAGAAAGCAAAACTTGGTTAATGATTTATCGCGTTGCCGTTGTCGGAATGGTATTCTTCGGATCAATC
GCTGCCCTTCAGACTGTTTGGAGCTTAGCTGATTTATTCATGGGACTAATGGTATTCACAAATTTAATTGCCATCTCATT
CCTAAGCAAGTTCGCCTACGCGGCATTAGTAGACTATATGAAGCAAAAGAAACAAGGAAAAGATCCTGTTTTCGTTGCAA
GCTCTATCCCTGGCTTACAAAATACAGAGTGCTGGGATGGACAGGATGTAGAAGAAAATAAAAAAGCTGTATAA

Upstream 100 bases:

>100_bases
ACATAAAACAGATTTAAGTAACATGATTTTTTAGAAAAATTGATTCTCGTATATTTTTTGCATATAATTTTCTTATTACG
GAAAAAAGGAAGTGTATTAT

Downstream 100 bases:

>100_bases
CAACTAAACGGTGAAGCGGTTCGTTCAGAACAAGAGTTATATATCAAACAAAATCCCTATTACTCTACAGTAATAGGGAT
TTTTATTTACACAGTTCATA

Product: sodium/alanine symporter family protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MVDIFSRFLEVTNNILWSYILIAMLIGFGLYFSFKLKFVQITHFGEMVGLISKGFNRKEKKKDSISPFQAFCLSAAARIG
IGNLAGVALAISMGGPGAVFWMWFIAILGAATSFVECTLAQIYKVKDGSRFRGGPAYYMEKGLNKRWMGIWFSLLITVAY
GLIFNSVQANTVTIAFENAFGLERTIVGALLALLVAVIIFGGIKSISRITEMIVPPMAIIYIGVAIFVVINNFTMLPSIF
MEIFNGAFGLDQAIAGGIGAAIKFGIQRGLFATEAGMGSSPNAAATSDVSHPVKQGLVQALGVFVDTFLVCTSTAFIVLC
SGLYKGTNLQGIELTQSALSSQIGPWASTFLAIIIFLFAFSSLLGNYYYGETNIAFIKESKTWLMIYRVAVVGMVFFGSI
AALQTVWSLADLFMGLMVFTNLIAISFLSKFAYAALVDYMKQKKQGKDPVFVASSIPGLQNTECWDGQDVEENKKAV

Sequences:

>Translated_477_residues
MVDIFSRFLEVTNNILWSYILIAMLIGFGLYFSFKLKFVQITHFGEMVGLISKGFNRKEKKKDSISPFQAFCLSAAARIG
IGNLAGVALAISMGGPGAVFWMWFIAILGAATSFVECTLAQIYKVKDGSRFRGGPAYYMEKGLNKRWMGIWFSLLITVAY
GLIFNSVQANTVTIAFENAFGLERTIVGALLALLVAVIIFGGIKSISRITEMIVPPMAIIYIGVAIFVVINNFTMLPSIF
MEIFNGAFGLDQAIAGGIGAAIKFGIQRGLFATEAGMGSSPNAAATSDVSHPVKQGLVQALGVFVDTFLVCTSTAFIVLC
SGLYKGTNLQGIELTQSALSSQIGPWASTFLAIIIFLFAFSSLLGNYYYGETNIAFIKESKTWLMIYRVAVVGMVFFGSI
AALQTVWSLADLFMGLMVFTNLIAISFLSKFAYAALVDYMKQKKQGKDPVFVASSIPGLQNTECWDGQDVEENKKAV
>Mature_477_residues
MVDIFSRFLEVTNNILWSYILIAMLIGFGLYFSFKLKFVQITHFGEMVGLISKGFNRKEKKKDSISPFQAFCLSAAARIG
IGNLAGVALAISMGGPGAVFWMWFIAILGAATSFVECTLAQIYKVKDGSRFRGGPAYYMEKGLNKRWMGIWFSLLITVAY
GLIFNSVQANTVTIAFENAFGLERTIVGALLALLVAVIIFGGIKSISRITEMIVPPMAIIYIGVAIFVVINNFTMLPSIF
MEIFNGAFGLDQAIAGGIGAAIKFGIQRGLFATEAGMGSSPNAAATSDVSHPVKQGLVQALGVFVDTFLVCTSTAFIVLC
SGLYKGTNLQGIELTQSALSSQIGPWASTFLAIIIFLFAFSSLLGNYYYGETNIAFIKESKTWLMIYRVAVVGMVFFGSI
AALQTVWSLADLFMGLMVFTNLIAISFLSKFAYAALVDYMKQKKQGKDPVFVASSIPGLQNTECWDGQDVEENKKAV

Specific function: Probably functions as a sodium/glutamine symporter for glutamine uptake [H]

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=485, Percent_Identity=40.4123711340206, Blast_Score=338, Evalue=4e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 51846; Mature: 51846

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDIFSRFLEVTNNILWSYILIAMLIGFGLYFSFKLKFVQITHFGEMVGLISKGFNRKEK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHCCCCHHH
KKDSISPFQAFCLSAAARIGIGNLAGVALAISMGGPGAVFWMWFIAILGAATSFVECTLA
HHCCCCHHHHHHHHHHHHHCCCHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
QIYKVKDGSRFRGGPAYYMEKGLNKRWMGIWFSLLITVAYGLIFNSVQANTVTIAFENAF
HHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
GLERTIVGALLALLVAVIIFGGIKSISRITEMIVPPMAIIYIGVAIFVVINNFTMLPSIF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCHHHHHHHH
MEIFNGAFGLDQAIAGGIGAAIKFGIQRGLFATEAGMGSSPNAAATSDVSHPVKQGLVQA
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
LGVFVDTFLVCTSTAFIVLCSGLYKGTNLQGIELTQSALSSQIGPWASTFLAIIIFLFAF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SSLLGNYYYGETNIAFIKESKTWLMIYRVAVVGMVFFGSIAALQTVWSLADLFMGLMVFT
HHHHCCCEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLIAISFLSKFAYAALVDYMKQKKQGKDPVFVASSIPGLQNTECWDGQDVEENKKAV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVDIFSRFLEVTNNILWSYILIAMLIGFGLYFSFKLKFVQITHFGEMVGLISKGFNRKEK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHCCCCHHH
KKDSISPFQAFCLSAAARIGIGNLAGVALAISMGGPGAVFWMWFIAILGAATSFVECTLA
HHCCCCHHHHHHHHHHHHHCCCHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
QIYKVKDGSRFRGGPAYYMEKGLNKRWMGIWFSLLITVAYGLIFNSVQANTVTIAFENAF
HHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
GLERTIVGALLALLVAVIIFGGIKSISRITEMIVPPMAIIYIGVAIFVVINNFTMLPSIF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCHHHHHHHH
MEIFNGAFGLDQAIAGGIGAAIKFGIQRGLFATEAGMGSSPNAAATSDVSHPVKQGLVQA
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
LGVFVDTFLVCTSTAFIVLCSGLYKGTNLQGIELTQSALSSQIGPWASTFLAIIIFLFAF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SSLLGNYYYGETNIAFIKESKTWLMIYRVAVVGMVFFGSIAALQTVWSLADLFMGLMVFT
HHHHCCCEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLIAISFLSKFAYAALVDYMKQKKQGKDPVFVASSIPGLQNTECWDGQDVEENKKAV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]