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The map label for this gene is nupC [H]

Identifier: 52140472

GI number: 52140472

Start: 4873365

End: 4874546

Strand: Reverse

Name: nupC [H]

Synonym: BCZK4785

Alternate gene names: 52140472

Gene position: 4874546-4873365 (Counterclockwise)

Preceding gene: 52140473

Following gene: 52140474

Centisome position: 91.96

GC content: 35.11

Gene sequence:

>1182_bases
ATGAAAATAGTAATGTTTCTAGTCGGTTTACTTGTAGTATTTGTACTAGGGTTCCTTATCAGTTCAGATCGCAAGAAAAT
TAAATATAAACCAATTGCACTTATGCTTGTCATTCAATTGGTACTTGCGTATTTCTTACTAAATACAAAGGTCGGATTTG
TATTAGTAAAAGGGATTGCAGATGGGTTTGGCGCTATTTTAAAATTTGCGGAAGCAGGGGTTAATTTCGTATTTGGTGGT
CTAGCAAATGATGGACAAGCACCATTCTTCTTAACAGTATTATTACCAATTATTTTCTTAGCAGTACTAATTGGGATCTT
ACAACATATTAAAATTTTACCGATTATCATTCGTGCAGTCGGTTTCCTATTAAGTAAAGTTAACGGTTTAGGAAAACTAG
AATCATATAATGCGGTAGCAGCTGCAATTGTTGGTCAAGGGGAAGTATTCATTACAGTAAAAGATCAATTAAGCAAACTA
CCGAAAAATCGTTTATACACACTTTGTGCATCTTCTATGTCAACAGTATCGATGTCAATCGTCGGTTCTTATATGAAAAT
GATTGATCCAAAATATGTAGTAACAGCACTTGTACTAAACTTATTCAGTGGATTCATTATCGTTCATATTATTAATCCAT
ATGATGTAAAAGAAGAAGACGACATTTTAGAATTACAAGAAGATAAAAAACAAACATTCTTTGAAATGTTAGGCGAATAT
ATTATGCTTGGTTTCTCTATCGCTGTAACAGTAGCGGCGATGTTAATCGGTTTCGTAGCATTAATTACAGCAATTAACGG
TGTATTCGATTCAATTTTCGGAATCACATTCCAAAGCATTTTAGGGTACATTTTCTCACCATTAGCATTCGTAATGGGCA
TCCCAACATCAGAGATGCTAACAGCAGGACAAATTATGGCAACGAAATTAGTAACGAACGAATTCGTTGCAATGCTTGAC
CTTGGTAAAGTAGCTGGTGACTTATCAGCTCGTACAGTAGGTATTTTATCTATCTTCCTTGTATCATTTGCGAACTTCTC
ATCTATCGGGATTATCGCAGGTGCAACGAAGAGTATCGATGGCAAACAAGCAAACGTTGTATCATCATTCGGCTTAAAAC
TTGTATACGGTGCAACGTTAGTAAGTATATTATCAGCGGTTATCGTTGGGGTTATGCTTTAA

Upstream 100 bases:

>100_bases
AGTATTTAGGGGTTTTAAATTTTTTTAACATCAATGGTCAGACGTCATTCGTTATACGTTATACGACCAATAACTCGTGG
GAGAAGTGGAGGATTCACAT

Downstream 100 bases:

>100_bases
TAAATAGTAAAGAGGTCGTTTATCTCTCGCCTAGAGGGATGAACGGCTTTTTTATTTTGGTATGATAAATAGTGTGGAGA
GGGGGAATTAGGGTGAAAGA

Product: NupC family nucleoside transporter

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKIVMFLVGLLVVFVLGFLISSDRKKIKYKPIALMLVIQLVLAYFLLNTKVGFVLVKGIADGFGAILKFAEAGVNFVFGG
LANDGQAPFFLTVLLPIIFLAVLIGILQHIKILPIIIRAVGFLLSKVNGLGKLESYNAVAAAIVGQGEVFITVKDQLSKL
PKNRLYTLCASSMSTVSMSIVGSYMKMIDPKYVVTALVLNLFSGFIIVHIINPYDVKEEDDILELQEDKKQTFFEMLGEY
IMLGFSIAVTVAAMLIGFVALITAINGVFDSIFGITFQSILGYIFSPLAFVMGIPTSEMLTAGQIMATKLVTNEFVAMLD
LGKVAGDLSARTVGILSIFLVSFANFSSIGIIAGATKSIDGKQANVVSSFGLKLVYGATLVSILSAVIVGVML

Sequences:

>Translated_393_residues
MKIVMFLVGLLVVFVLGFLISSDRKKIKYKPIALMLVIQLVLAYFLLNTKVGFVLVKGIADGFGAILKFAEAGVNFVFGG
LANDGQAPFFLTVLLPIIFLAVLIGILQHIKILPIIIRAVGFLLSKVNGLGKLESYNAVAAAIVGQGEVFITVKDQLSKL
PKNRLYTLCASSMSTVSMSIVGSYMKMIDPKYVVTALVLNLFSGFIIVHIINPYDVKEEDDILELQEDKKQTFFEMLGEY
IMLGFSIAVTVAAMLIGFVALITAINGVFDSIFGITFQSILGYIFSPLAFVMGIPTSEMLTAGQIMATKLVTNEFVAMLD
LGKVAGDLSARTVGILSIFLVSFANFSSIGIIAGATKSIDGKQANVVSSFGLKLVYGATLVSILSAVIVGVML
>Mature_393_residues
MKIVMFLVGLLVVFVLGFLISSDRKKIKYKPIALMLVIQLVLAYFLLNTKVGFVLVKGIADGFGAILKFAEAGVNFVFGG
LANDGQAPFFLTVLLPIIFLAVLIGILQHIKILPIIIRAVGFLLSKVNGLGKLESYNAVAAAIVGQGEVFITVKDQLSKL
PKNRLYTLCASSMSTVSMSIVGSYMKMIDPKYVVTALVLNLFSGFIIVHIINPYDVKEEDDILELQEDKKQTFFEMLGEY
IMLGFSIAVTVAAMLIGFVALITAINGVFDSIFGITFQSILGYIFSPLAFVMGIPTSEMLTAGQIMATKLVTNEFVAMLD
LGKVAGDLSARTVGILSIFLVSFANFSSIGIIAGATKSIDGKQANVVSSFGLKLVYGATLVSILSAVIVGVML

Specific function: Transports Nucleosides With A High Affinity Except Guanosine And Deoxyguanosine. Driven By A Proton Motive Force. [C]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI42542381, Length=411, Percent_Identity=25.0608272506083, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI11545853, Length=411, Percent_Identity=25.0608272506083, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI227116277, Length=411, Percent_Identity=22.8710462287105, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1788737, Length=398, Percent_Identity=58.7939698492462, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1788485, Length=410, Percent_Identity=31.7073170731707, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1788488, Length=410, Percent_Identity=31.219512195122, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17560276, Length=397, Percent_Identity=25.1889168765743, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71991794, Length=392, Percent_Identity=24.4897959183673, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25146537, Length=189, Percent_Identity=24.3386243386243, Blast_Score=65, Evalue=9e-11,
Organism=Drosophila melanogaster, GI45552519, Length=401, Percent_Identity=24.1895261845387, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI45552517, Length=401, Percent_Identity=24.1895261845387, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19921868, Length=401, Percent_Identity=24.1895261845387, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI281360430, Length=401, Percent_Identity=24.4389027431421, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 42190; Mature: 42190

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVMFLVGLLVVFVLGFLISSDRKKIKYKPIALMLVIQLVLAYFLLNTKVGFVLVKGIA
CHHHHHHHHHHHHHHHHHHHCCCCCHHEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DGFGAILKFAEAGVNFVFGGLANDGQAPFFLTVLLPIIFLAVLIGILQHIKILPIIIRAV
HHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFLLSKVNGLGKLESYNAVAAAIVGQGEVFITVKDQLSKLPKNRLYTLCASSMSTVSMSI
HHHHHHHCCCCCCHHHHHHHHHEECCCEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHH
VGSYMKMIDPKYVVTALVLNLFSGFIIVHIINPYDVKEEDDILELQEDKKQTFFEMLGEY
HHHHHHHHCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IMLGFSIAVTVAAMLIGFVALITAINGVFDSIFGITFQSILGYIFSPLAFVMGIPTSEML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
TAGQIMATKLVTNEFVAMLDLGKVAGDLSARTVGILSIFLVSFANFSSIGIIAGATKSID
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
GKQANVVSSFGLKLVYGATLVSILSAVIVGVML
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIVMFLVGLLVVFVLGFLISSDRKKIKYKPIALMLVIQLVLAYFLLNTKVGFVLVKGIA
CHHHHHHHHHHHHHHHHHHHCCCCCHHEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DGFGAILKFAEAGVNFVFGGLANDGQAPFFLTVLLPIIFLAVLIGILQHIKILPIIIRAV
HHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFLLSKVNGLGKLESYNAVAAAIVGQGEVFITVKDQLSKLPKNRLYTLCASSMSTVSMSI
HHHHHHHCCCCCCHHHHHHHHHEECCCEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHH
VGSYMKMIDPKYVVTALVLNLFSGFIIVHIINPYDVKEEDDILELQEDKKQTFFEMLGEY
HHHHHHHHCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IMLGFSIAVTVAAMLIGFVALITAINGVFDSIFGITFQSILGYIFSPLAFVMGIPTSEML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
TAGQIMATKLVTNEFVAMLDLGKVAGDLSARTVGILSIFLVSFANFSSIGIIAGATKSID
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
GKQANVVSSFGLKLVYGATLVSILSAVIVGVML
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8550462; 8867804; 9384377 [H]