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The map label for this gene is glnT [H]

Identifier: 52140471

GI number: 52140471

Start: 4876377

End: 4877816

Strand: Reverse

Name: glnT [H]

Synonym: BCZK4787

Alternate gene names: 52140471

Gene position: 4877816-4876377 (Counterclockwise)

Preceding gene: 52140470

Following gene: 52140473

Centisome position: 92.02

GC content: 38.89

Gene sequence:

>1440_bases
ATGGATTTTTTAGCTAAGGTAATTGGGGATGTGAATAATGTCCTTTGGTCATATATCATTATTGCGATGTTAATTGGGTT
AGGACTCTACTTTTCATTTCGTGTGAAATTTGTCCAAGTTCGTTACTTCGGTGAAATGATTCGATTGCTTGGGGATGGGG
CAAGCTCGAAAACGAGGAAGGCGCAAAAAGAGAAGAGCGGTGTATCGTCATTCCAAGCATTTTGTATGAGTACAGCTTCT
CGTGTAGGTACTGGTAACTTAGCTGGTGTAGCTATCGCAATCTCAGCAGGTGGACCAGGCGCAGTATTTTGGATGTGGTT
AATCGCGGTAATTGGGGGTGCTTCTGCATTTGTAGAGAGCACATTAGCGCAAATTTATAAAGTAAAAGATGGGAATACGT
TCCGTGGTGGCCCAGCGTATTATATGGAAAAGGGTTTAAATAAACGCTGGCTTGGAGCTATTTTCTCTGTATTAATAACA
GTAAGTTTCGGATTAATTTTTAACGCTGTACAATCTAATACTGTAGCAGCTGCTTTTGATGGGGCATTCAAAACAGATAG
TAGATTAGTAGGTCTTGTAATGGCTGGTCTACTTGCAGTTATTATTTTCGGCGGAGTAAAACGAATTGCTCGCGCGGTTG
AAATGATCGTTCCAGTAATGGCAATTATTTATGTAGCAGTGGCATTATTCGTTGTTGTAACAAACATTACGGCAATTCCA
TATGTGTTTAAAGAAATTTTCTTACATGCTTTTGGAATTAAAGAAGTAGTAGGCGGGGGATTAGGAGCTGCGATTTTACT
AGGGGTAAAACGTGGATTATTCTCAAATGAAGCTGGTATGGGTAGTGCGCCGAACGCGGCTGCGACTGCAAACGTAACGC
ACCCAGTTAAACAAGGTTTCATTCAAACTTTAGGGGTATTTACAGATACATTATTAATTTGTAGTTGTACAGCATTTATC
ATTCTTTTATCAGATGTGCACAATGCAGCTGATTTAAATGGTATTCAATTAACGCAGCAGGCGTTAAGTCAACATATCGG
TCCATGGGCTTCTATATTTGTAGCAGTGGCAATCTTCTTGTTTGCATTCAGTTCATTAGTAGGTAACTACTACTATGGGG
AAACAAATATCGAGTTCTTAAATGGAAGTAGAGTACTATTAAATGCATACCGCATTGCTGTACTTGGTATGGTTATGTTA
GGTTCTGTAGCAACAATTCAAATCGTTTGGGATTTAGCAGATTTATTCATGGGTATTATGGCTGTTATTAACTTAGTGGC
AATCGTATTCCTTTCTAAGTACGCTTTTGCGGCATTATCAGATTATGTAAAGCAAAAGAAACAAGGAAAAGATCCAGTCT
TCTATGCAAATTCTATTCCAGGTCTGAAAAATACAGAGTGTTGGGAAGAGCAGGTAGTAGAGAAAGAAAAAGCGATATAA

Upstream 100 bases:

>100_bases
TTAACTCTCTCATGTTAGGAAACCTAACATAAAGTTCTTGATTACTAAGAGTTTTTAGAATATAATTTTCTTATTGTTAG
GAAGAGGGGGCAATGCAATT

Downstream 100 bases:

>100_bases
TGAGGTGAGCATCAAGTGAAACTTGATGCTCATTTTTTTGTTTTATTTAGCTATTTGTTAGAAGTAAAAGACGAACATTA
ATGGGTGTTTCTTAATAAAA

Product: sodium/alanine symporter family protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MDFLAKVIGDVNNVLWSYIIIAMLIGLGLYFSFRVKFVQVRYFGEMIRLLGDGASSKTRKAQKEKSGVSSFQAFCMSTAS
RVGTGNLAGVAIAISAGGPGAVFWMWLIAVIGGASAFVESTLAQIYKVKDGNTFRGGPAYYMEKGLNKRWLGAIFSVLIT
VSFGLIFNAVQSNTVAAAFDGAFKTDSRLVGLVMAGLLAVIIFGGVKRIARAVEMIVPVMAIIYVAVALFVVVTNITAIP
YVFKEIFLHAFGIKEVVGGGLGAAILLGVKRGLFSNEAGMGSAPNAAATANVTHPVKQGFIQTLGVFTDTLLICSCTAFI
ILLSDVHNAADLNGIQLTQQALSQHIGPWASIFVAVAIFLFAFSSLVGNYYYGETNIEFLNGSRVLLNAYRIAVLGMVML
GSVATIQIVWDLADLFMGIMAVINLVAIVFLSKYAFAALSDYVKQKKQGKDPVFYANSIPGLKNTECWEEQVVEKEKAI

Sequences:

>Translated_479_residues
MDFLAKVIGDVNNVLWSYIIIAMLIGLGLYFSFRVKFVQVRYFGEMIRLLGDGASSKTRKAQKEKSGVSSFQAFCMSTAS
RVGTGNLAGVAIAISAGGPGAVFWMWLIAVIGGASAFVESTLAQIYKVKDGNTFRGGPAYYMEKGLNKRWLGAIFSVLIT
VSFGLIFNAVQSNTVAAAFDGAFKTDSRLVGLVMAGLLAVIIFGGVKRIARAVEMIVPVMAIIYVAVALFVVVTNITAIP
YVFKEIFLHAFGIKEVVGGGLGAAILLGVKRGLFSNEAGMGSAPNAAATANVTHPVKQGFIQTLGVFTDTLLICSCTAFI
ILLSDVHNAADLNGIQLTQQALSQHIGPWASIFVAVAIFLFAFSSLVGNYYYGETNIEFLNGSRVLLNAYRIAVLGMVML
GSVATIQIVWDLADLFMGIMAVINLVAIVFLSKYAFAALSDYVKQKKQGKDPVFYANSIPGLKNTECWEEQVVEKEKAI
>Mature_479_residues
MDFLAKVIGDVNNVLWSYIIIAMLIGLGLYFSFRVKFVQVRYFGEMIRLLGDGASSKTRKAQKEKSGVSSFQAFCMSTAS
RVGTGNLAGVAIAISAGGPGAVFWMWLIAVIGGASAFVESTLAQIYKVKDGNTFRGGPAYYMEKGLNKRWLGAIFSVLIT
VSFGLIFNAVQSNTVAAAFDGAFKTDSRLVGLVMAGLLAVIIFGGVKRIARAVEMIVPVMAIIYVAVALFVVVTNITAIP
YVFKEIFLHAFGIKEVVGGGLGAAILLGVKRGLFSNEAGMGSAPNAAATANVTHPVKQGFIQTLGVFTDTLLICSCTAFI
ILLSDVHNAADLNGIQLTQQALSQHIGPWASIFVAVAIFLFAFSSLVGNYYYGETNIEFLNGSRVLLNAYRIAVLGMVML
GSVATIQIVWDLADLFMGIMAVINLVAIVFLSKYAFAALSDYVKQKKQGKDPVFYANSIPGLKNTECWEEQVVEKEKAI

Specific function: Probably functions as a sodium/glutamine symporter for glutamine uptake [H]

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=473, Percent_Identity=42.7061310782241, Blast_Score=354, Evalue=7e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 51393; Mature: 51393

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFLAKVIGDVNNVLWSYIIIAMLIGLGLYFSFRVKFVQVRYFGEMIRLLGDGASSKTRK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AQKEKSGVSSFQAFCMSTASRVGTGNLAGVAIAISAGGPGAVFWMWLIAVIGGASAFVES
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHH
TLAQIYKVKDGNTFRGGPAYYMEKGLNKRWLGAIFSVLITVSFGLIFNAVQSNTVAAAFD
HHHHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
GAFKTDSRLVGLVMAGLLAVIIFGGVKRIARAVEMIVPVMAIIYVAVALFVVVTNITAIP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVFKEIFLHAFGIKEVVGGGLGAAILLGVKRGLFSNEAGMGSAPNAAATANVTHPVKQGF
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
IQTLGVFTDTLLICSCTAFIILLSDVHNAADLNGIQLTQQALSQHIGPWASIFVAVAIFL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
FAFSSLVGNYYYGETNIEFLNGSRVLLNAYRIAVLGMVMLGSVATIQIVWDLADLFMGIM
HHHHHHHCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AVINLVAIVFLSKYAFAALSDYVKQKKQGKDPVFYANSIPGLKNTECWEEQVVEKEKAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MDFLAKVIGDVNNVLWSYIIIAMLIGLGLYFSFRVKFVQVRYFGEMIRLLGDGASSKTRK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AQKEKSGVSSFQAFCMSTASRVGTGNLAGVAIAISAGGPGAVFWMWLIAVIGGASAFVES
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHH
TLAQIYKVKDGNTFRGGPAYYMEKGLNKRWLGAIFSVLITVSFGLIFNAVQSNTVAAAFD
HHHHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
GAFKTDSRLVGLVMAGLLAVIIFGGVKRIARAVEMIVPVMAIIYVAVALFVVVTNITAIP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVFKEIFLHAFGIKEVVGGGLGAAILLGVKRGLFSNEAGMGSAPNAAATANVTHPVKQGF
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
IQTLGVFTDTLLICSCTAFIILLSDVHNAADLNGIQLTQQALSQHIGPWASIFVAVAIFL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
FAFSSLVGNYYYGETNIEFLNGSRVLLNAYRIAVLGMVMLGSVATIQIVWDLADLFMGIM
HHHHHHHCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AVINLVAIVFLSKYAFAALSDYVKQKKQGKDPVFYANSIPGLKNTECWEEQVVEKEKAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]