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The map label for this gene is vacB [H]

Identifier: 52140440

GI number: 52140440

Start: 4910465

End: 4912897

Strand: Reverse

Name: vacB [H]

Synonym: BCZK4818

Alternate gene names: 52140440

Gene position: 4912897-4910465 (Counterclockwise)

Preceding gene: 52140439

Following gene: 52140442

Centisome position: 92.68

GC content: 41.14

Gene sequence:

>2433_bases
TTGGAAGAAATCATACAAGAACATATTGATAAGTTGTTATTATTTATGAGAGAAGAAGCGTATAAACCGCTAACGATACA
AGAGTTAGAAGAGGCATTTGGGATTGAAGGTTCTGAGGGCTTTAAAGATTTCGTAAAGGCACTTGTAACGATGGAAGAAA
AGGGACTTGTTATTCGTACTCGTAGCAACCGTTACGGTCTTCCTGAAAAGATGAATTTAGTACGTGGTAAGTTAATTGGA
CATGCACGTGGCTTTGCATTCGTTGTACCAGACGAGAAGAAAACAGGAGATGATGATCTTTTCATCCCACCTACAGAATT
AAACGGTGCACTTCATGGCGATACAGTATTAGCACGCCTTAGTTCCCAATCGAGTGGTTCGCGTCAAGAAGGTTCCATTG
TACGCATTTTAGAGCGTGGCACGAAAGAATTAGTTGGTACATATACAGAATCGAAAAACTTTGGATTTGTTATACCTGAC
AATAAACGTTGGACAAGTGACATTTTCGTATTGAAAAGCGCATCAATGGGCGCTGTAGAAGGTCATAAAGTAGTTGTGAA
AATTACGAGCTATCCAGAGAATCGTTTAAGTGCAGAAGGTGAAGTTATTCAAATTCTAGGTCATAAAAATGATCCAGGAG
TAGATATTTTATCTGTCATTCATAAACATCACTTGCCTTTAGCGTTCCCTGAAGAAGTGATGGAGCACGCAAACAGTGTA
CCAGAAACGATTTCAGAAGAAGATTTAAAAGATCGCCGTGACCTACGTGACCAAATGATCGTAACAATTGACGGTGCAGA
CGCGAAAGATTTAGATGATGCTGTTACAGTAACGAAGCTCGAGAACGGTAACTATAAGCTTGGCGTTCACATTGCGGATG
TAAGCCATTACGTTCAAGAAGGTTCTCCAATTGATGTAGAAGCAGCGGAAAGAGCGACGAGTGTATATCTTGTTGACCGT
GTAATTCCAATGATTCCACATCGTCTATCTAACGGTATTTGTTCATTAAATCCGAAAGTAGACCGTCTGACGTTATCTTG
TGAAATGGAAATCAACAACTTAGGTGACGTTGTCAAACACGAGATTTTCCAAAGTGTAATTAAAACGACAGAGCGTATGA
CGTATGCTGACGTAAGAAGCATTTTAGAAGATGAGGACGAAGAATTAATTAAGCGCTATGAACCGTTAGTACCGATGTTT
AAAGAAATGGGCCAATTGGCACAAATTTTACGTGAAAAACGTATGCGCCGCGGTGCAATCGACTTTGACTTTAAAGAAGC
GAAAGTATTAGTAGATGAAGAAGGAAAACCGACAGATGTTGTTATGCGTGATCGTTCTGTATCAGAGAAGTTAATTGAAG
AATTTATGCTTGTTGCGAACGAAACAGTAGCAGAGCACTTCCACTGGATGAACGTACCATTCATGTATCGTGTCCATGAA
GATCCGAAAGAAGATAAGTTAGAGCGTTTCTTCGAGTTTGTAACGAACTTCGGATATGCGGTAAAAGGACGTGCGAATGA
AGTGCATCCTCGTGCGCTGCAACAAATTCTTGAAATGGTTCAAGGACAGCCTGAAGAAGTAGTCATTTCAACAGTTATGC
TTCGCTCAATGAAGCAAGCACGTTACGATGCAGATAGCTTAGGACATTTCGGTTTATCAACGGAGTTCTACACGCATTTC
ACATCACCAATTCGTCGTTATCCAGATACGATTGTTCATAGATTAATTCGTGAATACATCATTAACGGTAAAGTCGACAA
TGAAACGCAAGCAAAATGGCGTGAAAAATTACCTGAGATTGCAGAACACTCTTCTAATATGGAACGTCGTGCTGTTGAAG
CAGAACGTGAAACAGACGAAATGAAAAAAGCAGAGTACATGGTTGATAAGATCGGCGAAGAGTATGACGGTATGATTAGC
TCTGTAACAAACTTCGGTTTATTCGTAGAGCTTCCAAATACAATTGAAGGTCTTGTACATGTTAGCTACTTAACGGATGA
TTACTACCGTTATGATGAGCAGCATTTCGCAATGATTGGAGAACGTACAGGTAACGTATTCCGCATTGGTGACGAAATTA
CAATTCGCGTTATTAACGTAAACAAAGACGAGCGCGCAATTGACTTTGAAATCGTTGGCATGAAAGGTACGCCTCGTCGT
AAGTTCAAAGACCGCCCAGTCGTTATCGAACAGCCAAGAACAGGCAGAAAGAAACGCGGTGGACGTAGCGAGCGTAGTAA
TGAGCGCGGCGGAGAACGTGGCACAGGAAGAAAATTTGACCGTGGTGGCAAAGGAAAAGGAAGAGGATCTGCATCCGCAT
CCACATCTACGTCCGCTAGCCAGCCAGGGAAAAAAGATGGTAACGGCAAGAAGAAAAAAGCATTCTTCGAAAACGTACCA
GGATTCAAGAAGAAAAAGAAAAAGCGTAAGTAA

Upstream 100 bases:

>100_bases
ACTGTAAAAATCATAAGGGAAGTATTGAAGTTTCGCTTTATACATCCCCTATCTTTCGGATACACTAAATAATATAAGGT
TTTAAAGGAGGTATTTTTGC

Downstream 100 bases:

>100_bases
GAAAAGGATGGCTTCGCTTTGATAAAAAGCGAGGCTTTTTCTTTTTTATTGGTGGCTTGAGAGCGAGCTACTTTCGCTTT
TTGTTGGAATCCAGCTACAG

Product: ribonuclease R (RNase R)

Products: NA

Alternate protein names: RNase R; VacB protein homolog [H]

Number of amino acids: Translated: 810; Mature: 810

Protein sequence:

>810_residues
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRTRSNRYGLPEKMNLVRGKLIG
HARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARLSSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPD
NKRWTSDIFVLKSASMGAVEGHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQEGSPIDVEAAERATSVYLVDR
VIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKHEIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMF
KEMGQLAQILREKRMRRGAIDFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQARYDADSLGHFGLSTEFYTHF
TSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEIAEHSSNMERRAVEAERETDEMKKAEYMVDKIGEEYDGMIS
SVTNFGLFVELPNTIEGLVHVSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASTSTSASQPGKKDGNGKKKKAFFENVP
GFKKKKKKRK

Sequences:

>Translated_810_residues
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRTRSNRYGLPEKMNLVRGKLIG
HARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARLSSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPD
NKRWTSDIFVLKSASMGAVEGHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQEGSPIDVEAAERATSVYLVDR
VIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKHEIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMF
KEMGQLAQILREKRMRRGAIDFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQARYDADSLGHFGLSTEFYTHF
TSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEIAEHSSNMERRAVEAERETDEMKKAEYMVDKIGEEYDGMIS
SVTNFGLFVELPNTIEGLVHVSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASTSTSASQPGKKDGNGKKKKAFFENVP
GFKKKKKKRK
>Mature_810_residues
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRTRSNRYGLPEKMNLVRGKLIG
HARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARLSSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPD
NKRWTSDIFVLKSASMGAVEGHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQEGSPIDVEAAERATSVYLVDR
VIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKHEIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMF
KEMGQLAQILREKRMRRGAIDFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQARYDADSLGHFGLSTEFYTHF
TSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEIAEHSSNMERRAVEAERETDEMKKAEYMVDKIGEEYDGMIS
SVTNFGLFVELPNTIEGLVHVSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASTSTSASQPGKKDGNGKKKKAFFENVP
GFKKKKKKRK

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI190014623, Length=462, Percent_Identity=30.952380952381, Blast_Score=227, Evalue=4e-59,
Organism=Homo sapiens, GI190014625, Length=462, Percent_Identity=30.952380952381, Blast_Score=227, Evalue=4e-59,
Organism=Homo sapiens, GI134288890, Length=414, Percent_Identity=34.0579710144928, Blast_Score=218, Evalue=2e-56,
Organism=Homo sapiens, GI19115966, Length=575, Percent_Identity=28, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI219521928, Length=575, Percent_Identity=28, Blast_Score=191, Evalue=3e-48,
Organism=Escherichia coli, GI87082383, Length=725, Percent_Identity=38.7586206896552, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI1787542, Length=639, Percent_Identity=24.8826291079812, Blast_Score=148, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17553506, Length=538, Percent_Identity=30.1115241635688, Blast_Score=205, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI212645896, Length=492, Percent_Identity=28.6585365853659, Blast_Score=189, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6324552, Length=589, Percent_Identity=28.0135823429542, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6320499, Length=422, Percent_Identity=25.5924170616114, Blast_Score=107, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6323943, Length=432, Percent_Identity=22.9166666666667, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24649634, Length=475, Percent_Identity=31.1578947368421, Blast_Score=235, Evalue=8e-62,
Organism=Drosophila melanogaster, GI19922976, Length=363, Percent_Identity=32.5068870523416, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24654597, Length=363, Percent_Identity=32.5068870523416, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24654592, Length=363, Percent_Identity=32.5068870523416, Blast_Score=184, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805 [H]

Pfam domain/function: PF08206 OB_RNB; PF00773 RNB; PF00575 S1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 92251; Mature: 92251

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50126 S1 ; PS01175 RIBONUCLEASE_II ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRT
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEE
RSNRYGLPEKMNLVRGKLIGHARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARL
CCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCHHCCCCCCHHHHHHHH
SSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPDNKRWTSDIFVLKSASMGAVE
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEECCCCCCCC
GHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
CCEEEEEEECCCCHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCC
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQE
CHHCCHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEEEEECCCEEEEEEEHHHHHHHHC
GSPIDVEAAERATSVYLVDRVIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEEEEEHHHHHHHHHH
EIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMFKEMGQLAQILREKRMRRGAI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
DFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
CCCCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQA
CCCHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RYDADSLGHFGLSTEFYTHFTSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEI
HCCHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AEHSSNMERRAVEAERETDEMKKAEYMVDKIGEEYDGMISSVTNFGLFVELPNTIEGLVH
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCEEEECCHHHHHHEE
VSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
EHHHHHHHHHHCCHHHHHHHCCCCCEEEECCEEEEEEEECCCCCCEEEEEEEECCCCCCC
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASTSTSAS
CCCCCCEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QPGKKDGNGKKKKAFFENVPGFKKKKKKRK
CCCCCCCCCCHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure
MEEIIQEHIDKLLLFMREEAYKPLTIQELEEAFGIEGSEGFKDFVKALVTMEEKGLVIRT
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEE
RSNRYGLPEKMNLVRGKLIGHARGFAFVVPDEKKTGDDDLFIPPTELNGALHGDTVLARL
CCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCHHCCCCCCHHHHHHHH
SSQSSGSRQEGSIVRILERGTKELVGTYTESKNFGFVIPDNKRWTSDIFVLKSASMGAVE
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEECCCCCCCC
GHKVVVKITSYPENRLSAEGEVIQILGHKNDPGVDILSVIHKHHLPLAFPEEVMEHANSV
CCEEEEEEECCCCHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCC
PETISEEDLKDRRDLRDQMIVTIDGADAKDLDDAVTVTKLENGNYKLGVHIADVSHYVQE
CHHCCHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEEEEECCCEEEEEEEHHHHHHHHC
GSPIDVEAAERATSVYLVDRVIPMIPHRLSNGICSLNPKVDRLTLSCEMEINNLGDVVKH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEEEEEHHHHHHHHHH
EIFQSVIKTTERMTYADVRSILEDEDEELIKRYEPLVPMFKEMGQLAQILREKRMRRGAI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
DFDFKEAKVLVDEEGKPTDVVMRDRSVSEKLIEEFMLVANETVAEHFHWMNVPFMYRVHE
CCCCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DPKEDKLERFFEFVTNFGYAVKGRANEVHPRALQQILEMVQGQPEEVVISTVMLRSMKQA
CCCHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RYDADSLGHFGLSTEFYTHFTSPIRRYPDTIVHRLIREYIINGKVDNETQAKWREKLPEI
HCCHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AEHSSNMERRAVEAERETDEMKKAEYMVDKIGEEYDGMISSVTNFGLFVELPNTIEGLVH
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCEEEECCHHHHHHEE
VSYLTDDYYRYDEQHFAMIGERTGNVFRIGDEITIRVINVNKDERAIDFEIVGMKGTPRR
EHHHHHHHHHHCCHHHHHHHCCCCCEEEECCEEEEEEEECCCCCCEEEEEEEECCCCCCC
KFKDRPVVIEQPRTGRKKRGGRSERSNERGGERGTGRKFDRGGKGKGRGSASASTSTSAS
CCCCCCEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QPGKKDGNGKKKKAFFENVPGFKKKKKKRK
CCCCCCCCCCHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]