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The map label for this gene is rihA [H]

Identifier: 52140435

GI number: 52140435

Start: 4915751

End: 4916701

Strand: Direct

Name: rihA [H]

Synonym: BCZK4823

Alternate gene names: 52140435

Gene position: 4915751-4916701 (Clockwise)

Preceding gene: 52140449

Following gene: 52140423

Centisome position: 92.73

GC content: 38.07

Gene sequence:

>951_bases
ATGCCCAAAAAAGTTCTTATTTTTTGTGATCCTGGCATTGATGATACGATGGCTCTCCTCTTGGCATTCTTTATCGATGA
AATAGAAATCATCGGCATTGTTGCTGATTACGGCAATGTCCCAAAAGAAATGGCAGTACAAAATGCTCATTTTCTTACGA
ACGAAACAAGGAATAGAAATATTAAGATATTTGGTGGATCAGAGCGTCCGCTTACTGGTGCCCCGCCCGCTTTTTTTACA
GATGTACACGGAAAACAAGGTCTCGGGCCAATCATTCCAAAGGGGAATTTGGCTAACAGCGAAATGGAGAATTTCTTCGA
AGTGATTCCTCTTATTGAACAATATAAAGATGAATTAATTATTGTAAGTTTAGGAAGACTTACCTCTCTAGCAATTTTGT
TTATTATGTGCAAACAGTTAATGAAACAAATTAAATCTTACTACGTAATGGGCGGTGCCTTTTTACATCCTGGTAACGTT
ACCCCTATTTCCGAAGCAAACTTTTATGGCGATCCTACTGCTGCTAACATTGTTCTTCAATCCGCAGCTAACATGTACAT
ATACCCGTTAAACGTTACCCAATACTCCATCATTACACCAGAAATGGCCGAGTACATTGAAGCAAAAGGTAAAGCCCCAC
TCGTCAAACCGTTATTCGATCATTATTACTATGGATATTATAAAAATGCCCTGCCAGATTTAAAAGGTAGCCCCTTCCAT
GACACAATGCCAATACTCGCTTTACTTGATAATTCTATGTTTACGTATCACAAATCACCTATCGTTGTCATGGCAGAATC
TTATGCACAAGGAGCAAGCATTGGAGAATTTCGTTCCTTAGGAAAACCTAAACCATTTATGGATTGGCCGAGTCATCAAA
TCGCAATCGATTTTGATTATAACCGTTTCTTTAAACATTTCATGTCTCTTATGACTGATGAAGAATTTTAG

Upstream 100 bases:

>100_bases
GGGCAGCCCGACCCCACCTAAATTCTTTGCTTTCGCTGAATTTTGAGGTGGGGGTCTTACTGCCCATTAAAGTGGGATAA
ACAAGATAGGTGGTGAACAT

Downstream 100 bases:

>100_bases
CATAAAAAAACAGACCGTGATTTCTCACGGTCTGTCGTTATTTTCTACAATTATCGTTTGATGTTGTAGAAAGATTTGAT
ACCATCGTAAACAGCGATTT

Product: inosine-uridine preferring nucleoside hydrolase

Products: D-ribose; purine

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MPKKVLIFCDPGIDDTMALLLAFFIDEIEIIGIVADYGNVPKEMAVQNAHFLTNETRNRNIKIFGGSERPLTGAPPAFFT
DVHGKQGLGPIIPKGNLANSEMENFFEVIPLIEQYKDELIIVSLGRLTSLAILFIMCKQLMKQIKSYYVMGGAFLHPGNV
TPISEANFYGDPTAANIVLQSAANMYIYPLNVTQYSIITPEMAEYIEAKGKAPLVKPLFDHYYYGYYKNALPDLKGSPFH
DTMPILALLDNSMFTYHKSPIVVMAESYAQGASIGEFRSLGKPKPFMDWPSHQIAIDFDYNRFFKHFMSLMTDEEF

Sequences:

>Translated_316_residues
MPKKVLIFCDPGIDDTMALLLAFFIDEIEIIGIVADYGNVPKEMAVQNAHFLTNETRNRNIKIFGGSERPLTGAPPAFFT
DVHGKQGLGPIIPKGNLANSEMENFFEVIPLIEQYKDELIIVSLGRLTSLAILFIMCKQLMKQIKSYYVMGGAFLHPGNV
TPISEANFYGDPTAANIVLQSAANMYIYPLNVTQYSIITPEMAEYIEAKGKAPLVKPLFDHYYYGYYKNALPDLKGSPFH
DTMPILALLDNSMFTYHKSPIVVMAESYAQGASIGEFRSLGKPKPFMDWPSHQIAIDFDYNRFFKHFMSLMTDEEF
>Mature_315_residues
PKKVLIFCDPGIDDTMALLLAFFIDEIEIIGIVADYGNVPKEMAVQNAHFLTNETRNRNIKIFGGSERPLTGAPPAFFTD
VHGKQGLGPIIPKGNLANSEMENFFEVIPLIEQYKDELIIVSLGRLTSLAILFIMCKQLMKQIKSYYVMGGAFLHPGNVT
PISEANFYGDPTAANIVLQSAANMYIYPLNVTQYSIITPEMAEYIEAKGKAPLVKPLFDHYYYGYYKNALPDLKGSPFHD
TMPILALLDNSMFTYHKSPIVVMAESYAQGASIGEFRSLGKPKPFMDWPSHQIAIDFDYNRFFKHFMSLMTDEEF

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=307, Percent_Identity=29.6416938110749, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788486, Length=307, Percent_Identity=23.7785016286645, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786213, Length=302, Percent_Identity=27.8145695364238, Blast_Score=85, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI32565474, Length=187, Percent_Identity=27.807486631016, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17565698, Length=186, Percent_Identity=27.4193548387097, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320608, Length=258, Percent_Identity=30.2325581395349, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 35575; Mature: 35443

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKKVLIFCDPGIDDTMALLLAFFIDEIEIIGIVADYGNVPKEMAVQNAHFLTNETRNRN
CCCEEEEEECCCCCHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCE
IKIFGGSERPLTGAPPAFFTDVHGKQGLGPIIPKGNLANSEMENFFEVIPLIEQYKDELI
EEEECCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
IVSLGRLTSLAILFIMCKQLMKQIKSYYVMGGAFLHPGNVTPISEANFYGDPTAANIVLQ
EEEHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEECCCCCCCCCCCCCCCCCHHHHHHHC
SAANMYIYPLNVTQYSIITPEMAEYIEAKGKAPLVKPLFDHYYYGYYKNALPDLKGSPFH
CCCCEEEEEECCCEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
DTMPILALLDNSMFTYHKSPIVVMAESYAQGASIGEFRSLGKPKPFMDWPSHQIAIDFDY
HHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEECH
NRFFKHFMSLMTDEEF
HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PKKVLIFCDPGIDDTMALLLAFFIDEIEIIGIVADYGNVPKEMAVQNAHFLTNETRNRN
CCEEEEEECCCCCHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCE
IKIFGGSERPLTGAPPAFFTDVHGKQGLGPIIPKGNLANSEMENFFEVIPLIEQYKDELI
EEEECCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
IVSLGRLTSLAILFIMCKQLMKQIKSYYVMGGAFLHPGNVTPISEANFYGDPTAANIVLQ
EEEHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEECCCCCCCCCCCCCCCCCHHHHHHHC
SAANMYIYPLNVTQYSIITPEMAEYIEAKGKAPLVKPLFDHYYYGYYKNALPDLKGSPFH
CCCCEEEEEECCCEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
DTMPILALLDNSMFTYHKSPIVVMAESYAQGASIGEFRSLGKPKPFMDWPSHQIAIDFDY
HHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEECH
NRFFKHFMSLMTDEEF
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA