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The map label for this gene is arsB [H]

Identifier: 52140341

GI number: 52140341

Start: 5009391

End: 5010749

Strand: Direct

Name: arsB [H]

Synonym: BCZK4919

Alternate gene names: 52140341

Gene position: 5009391-5010749 (Clockwise)

Preceding gene: 52140343

Following gene: 52140338

Centisome position: 94.5

GC content: 36.87

Gene sequence:

>1359_bases
ATGAGCGTTGAAATATGGATTACTATTATTGTATTTTTCTTAACGATGATTGTTATCTTTTGGCGACCTCGTGGTTTAAA
TGAGGCGTGGCCGGCAGCAATTGGTGCTGGCATTATCCTGATTACTGGACTTGTATCAAAACCAGACGTTATGGACATTA
TTAGTAAAATTGGCGGTGCCTCCATAACAATATTAGCGACCATTGTTATGGCGGTTATACTAGAAAGCTTCGGCTTCTTC
CACTGGTCTGCGGCGAAACTTGCAAACTTAGCAAAAGGTTCCGGTCGCCGTTTATACTGGTATATTCAATTATTATGTTT
TCTTATGACTCTTTTATTTAATAACGACGGTAGTATTTTAATTACAACTCCAATTTTAATTCTTCTTCTAAAAAATCTTC
AATTAAAACCACATCAACAAATCCCTTATTTATTAAGCGGTGCTTTAATTGCTACTGCATCTAGTGCGCCAATTGGTGTA
AGTAATATCGTAAACTTAATCGCATTAAATATCGTGAATATGACTCTTTATATGCATACTGCTATGATGTTTGTACCTGC
AACATTAGGGCTGTTGTTTATGTCATGGCTTATGTATACGGTTTTGAAGAAAAAACTTCCGAAAAAATTGCCTGTTTCTT
CATATGATATCGAAGAAATATTCTTCACAAAGAACTTCCATCCGTTAAAAGGAAATAATTCTGTTGATACGAAACAAAAA
CGGACAAGATTTATGCTAAAAGTATTAGGCTTCGTCTTCCTTATGCGCTGTCTTCTATTCGTCGCATCCTTCTTATCTAT
CCCAATTGAAATTGTTGCCGTACTCGGCTCACTCGTTCTTCTCATCTGGAGATGGTACTACTTACGAACAAATCCTGTCG
ACATTTTGAAAAAAACACCGTGGCACATTTTAATTTTTGCTTTCTCTATGTACGTCATTATTTACGGGCTCCACAACGCA
GGATTAACAACAGCACTTGTAAATTGGCTCGAACCAGTTGTAAGTCAGCATCTACTTTATGCTAGCTTTGCAATGGGCGG
ACTCGTTTCCCTCCTCTCCAACATCTTCAATAATCACCCTGCACTTATGATTGGTACTATTACATTAACTGAAATGGGAC
TAGACCCGATTACATTAAAAACGATCTATCTCGCAAACATTATTGGTAGTGACATCGGTTCACTATTATTACCAATCGGT
ACGCTAGCTTCCCTCATTTGGATGTATATCCTGAGACAAAACAAAATTAAAGTAAAATGGAAAGATTATTTAAGTGTATC
CCTTATCGTCATTCCGCTCACAACAGTCGTCACATTATTTCTCTTATTCTATTGGGTACATCTCTTCTTCTCCTTATAA

Upstream 100 bases:

>100_bases
TTATACGTCTAGTTCCTATTACTTTTTGCTGAATATACTACCATTATAAACTTGAGACAAGCTACATCTGTTCAAGGGAA
AATAGAAAGGTAGGCAAAAT

Downstream 100 bases:

>100_bases
AGTGAAACTTTAATCAGTGGGGGTTTTGTTCATCCCCCACTGATTATCAGCCCTAACCAATCGGGCTGCCCACAAATAGC
GGGATAAACGACAAAAGCCC

Product: arsenical pump family protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSVEIWITIIVFFLTMIVIFWRPRGLNEAWPAAIGAGIILITGLVSKPDVMDIISKIGGASITILATIVMAVILESFGFF
HWSAAKLANLAKGSGRRLYWYIQLLCFLMTLLFNNDGSILITTPILILLLKNLQLKPHQQIPYLLSGALIATASSAPIGV
SNIVNLIALNIVNMTLYMHTAMMFVPATLGLLFMSWLMYTVLKKKLPKKLPVSSYDIEEIFFTKNFHPLKGNNSVDTKQK
RTRFMLKVLGFVFLMRCLLFVASFLSIPIEIVAVLGSLVLLIWRWYYLRTNPVDILKKTPWHILIFAFSMYVIIYGLHNA
GLTTALVNWLEPVVSQHLLYASFAMGGLVSLLSNIFNNHPALMIGTITLTEMGLDPITLKTIYLANIIGSDIGSLLLPIG
TLASLIWMYILRQNKIKVKWKDYLSVSLIVIPLTTVVTLFLLFYWVHLFFSL

Sequences:

>Translated_452_residues
MSVEIWITIIVFFLTMIVIFWRPRGLNEAWPAAIGAGIILITGLVSKPDVMDIISKIGGASITILATIVMAVILESFGFF
HWSAAKLANLAKGSGRRLYWYIQLLCFLMTLLFNNDGSILITTPILILLLKNLQLKPHQQIPYLLSGALIATASSAPIGV
SNIVNLIALNIVNMTLYMHTAMMFVPATLGLLFMSWLMYTVLKKKLPKKLPVSSYDIEEIFFTKNFHPLKGNNSVDTKQK
RTRFMLKVLGFVFLMRCLLFVASFLSIPIEIVAVLGSLVLLIWRWYYLRTNPVDILKKTPWHILIFAFSMYVIIYGLHNA
GLTTALVNWLEPVVSQHLLYASFAMGGLVSLLSNIFNNHPALMIGTITLTEMGLDPITLKTIYLANIIGSDIGSLLLPIG
TLASLIWMYILRQNKIKVKWKDYLSVSLIVIPLTTVVTLFLLFYWVHLFFSL
>Mature_451_residues
SVEIWITIIVFFLTMIVIFWRPRGLNEAWPAAIGAGIILITGLVSKPDVMDIISKIGGASITILATIVMAVILESFGFFH
WSAAKLANLAKGSGRRLYWYIQLLCFLMTLLFNNDGSILITTPILILLLKNLQLKPHQQIPYLLSGALIATASSAPIGVS
NIVNLIALNIVNMTLYMHTAMMFVPATLGLLFMSWLMYTVLKKKLPKKLPVSSYDIEEIFFTKNFHPLKGNNSVDTKQKR
TRFMLKVLGFVFLMRCLLFVASFLSIPIEIVAVLGSLVLLIWRWYYLRTNPVDILKKTPWHILIFAFSMYVIIYGLHNAG
LTTALVNWLEPVVSQHLLYASFAMGGLVSLLSNIFNNHPALMIGTITLTEMGLDPITLKTIYLANIIGSDIGSLLLPIGT
LASLIWMYILRQNKIKVKWKDYLSVSLIVIPLTTVVTLFLLFYWVHLFFSL

Specific function: Involved In Arsenical Resistance. Thought To Form The Channel Of An Arsenite Pump (By Similarity). [C]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=420, Percent_Identity=29.2857142857143, Blast_Score=199, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 50849; Mature: 50718

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEIWITIIVFFLTMIVIFWRPRGLNEAWPAAIGAGIILITGLVSKPDVMDIISKIGGA
CCEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCHHHHHHHCCCCCHHHHHHHHHCCC
SITILATIVMAVILESFGFFHWSAAKLANLAKGSGRRLYWYIQLLCFLMTLLFNNDGSIL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
ITTPILILLLKNLQLKPHQQIPYLLSGALIATASSAPIGVSNIVNLIALNIVNMTLYMHT
EHHHHHHHHHHHCCCCCCCCCCHHHHCHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
AMMFVPATLGLLFMSWLMYTVLKKKLPKKLPVSSYDIEEIFFTKNFHPLKGNNSVDTKQK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEECCCCCCCCCCCCCHHHH
RTRFMLKVLGFVFLMRCLLFVASFLSIPIEIVAVLGSLVLLIWRWYYLRTNPVDILKKTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
WHILIFAFSMYVIIYGLHNAGLTTALVNWLEPVVSQHLLYASFAMGGLVSLLSNIFNNHP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ALMIGTITLTEMGLDPITLKTIYLANIIGSDIGSLLLPIGTLASLIWMYILRQNKIKVKW
CEEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
KDYLSVSLIVIPLTTVVTLFLLFYWVHLFFSL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVEIWITIIVFFLTMIVIFWRPRGLNEAWPAAIGAGIILITGLVSKPDVMDIISKIGGA
CEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCHHHHHHHCCCCCHHHHHHHHHCCC
SITILATIVMAVILESFGFFHWSAAKLANLAKGSGRRLYWYIQLLCFLMTLLFNNDGSIL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
ITTPILILLLKNLQLKPHQQIPYLLSGALIATASSAPIGVSNIVNLIALNIVNMTLYMHT
EHHHHHHHHHHHCCCCCCCCCCHHHHCHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
AMMFVPATLGLLFMSWLMYTVLKKKLPKKLPVSSYDIEEIFFTKNFHPLKGNNSVDTKQK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEECCCCCCCCCCCCCHHHH
RTRFMLKVLGFVFLMRCLLFVASFLSIPIEIVAVLGSLVLLIWRWYYLRTNPVDILKKTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
WHILIFAFSMYVIIYGLHNAGLTTALVNWLEPVVSQHLLYASFAMGGLVSLLSNIFNNHP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ALMIGTITLTEMGLDPITLKTIYLANIIGSDIGSLLLPIGTLASLIWMYILRQNKIKVKW
CEEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
KDYLSVSLIVIPLTTVVTLFLLFYWVHLFFSL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]