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The map label for this gene is holB [H]

Identifier: 51894391

GI number: 51894391

Start: 3475975

End: 3476973

Strand: Reverse

Name: holB [H]

Synonym: STH3256

Alternate gene names: 51894391

Gene position: 3476973-3475975 (Counterclockwise)

Preceding gene: 51894392

Following gene: 51894390

Centisome position: 97.5

GC content: 70.97

Gene sequence:

>999_bases
GTGACCTGGTCTGCGATTCGGGGTCAGCGCATGGCCGTGGACCTGCTGCGCCGGGCAGTGGCTGGCGGCAGAGTGGCTCA
CGCCTACCTGTTCGCGGGCCCGGCGGGGGTGGGCAAGCGGCTCGTCGCCCTTGAGCTGGCCCGCAGTCTCAACTGTCTCG
AACCGCGGGCGGGGGAGGCCTGCGGCGCCTGCCGGTCATGTCTGAAGCTGTCGGCGGAGCCACCGGTCCATCCGGACGTG
ACGGTGGTCGAACCCGAGGGCCGGATGATCCGCACCGAGCAGATGCGGCAGGTGCAGAACGAGGTGTACGCCCGCCCGAC
GGAAGGGCGCTACAAAGTCGTCATCATCGACGGGGCCGAGCGCATGAACGCCGAGTCCGGCAACCGGCTCCTGAAGGTCC
TGGAGGAGCCCCCGCCTTACGCCTGTTTCGTCCTGCTGACGACCAACGCGGCCGGCGTACTGCCGACCATCGTGTCCCGG
TGCCAGATCGTCCACTTCTCGCCCCTGCCCGCGGAGGCGATCGCGGCCGTGCTGCAGGAAAAGGCCGGCATCGGCCCCGG
CGAGGCCCGCCTGTTCGCCTCGCTCTCGGGTGGGTCGGTGGGGGCGGCCCTCGCGATGGCCGCCAACCCGGAGGTCGGCC
GGCGTCGCGACGAGGCGCTCAGCCTGCTGTTGCGCCTCGGGGAGATGGACGACGCCGACCTGCTGGAGCAGGCGGCGGCG
TGGGAAAAGGAGAAAGATCACCTGGAGGAGCGGCTGGAGATGGCTGCCTTCTGGCTGCGGGATGCCCTCCTGGTCGCCCG
GGAGGCCCCGGAACGGCTGGTTGTCAACGCAGACCGGCTGCCGGAGGTGCGCACCCTGGCCGGCCGGTACGGTGGCGAGG
GGCTGGTGGCGGCCCTGGAGAGCCTTTCCCGTGCGCGGGACGCCCTTCTCAGAAACGGCAACGCGCGGCTCGTGCTGGAC
GTGCTGATGCTGGATCTGCACGGCGCGGCCCGATTCTGA

Upstream 100 bases:

>100_bases
GGACTCGTACATTCCGTATCCGGTGGAGATCACCGTGGGCGGCGCCATCATCTTCGTGCTCCAGGTGGAGCGCTTCGAGC
AGTTCTAGGAGGACACAGGC

Downstream 100 bases:

>100_bases
GCAGGATTCATTCTGAGCAGGATTCATTCCCAGGAGATCATAACGGAGAGGCCCCACGAGCGCATGATGGACGCTGCCGC
GGGCAGCGAGGAGGTGACGG

Product: DNA polymerase III delta' subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTWSAIRGQRMAVDLLRRAVAGGRVAHAYLFAGPAGVGKRLVALELARSLNCLEPRAGEACGACRSCLKLSAEPPVHPDV
TVVEPEGRMIRTEQMRQVQNEVYARPTEGRYKVVIIDGAERMNAESGNRLLKVLEEPPPYACFVLLTTNAAGVLPTIVSR
CQIVHFSPLPAEAIAAVLQEKAGIGPGEARLFASLSGGSVGAALAMAANPEVGRRRDEALSLLLRLGEMDDADLLEQAAA
WEKEKDHLEERLEMAAFWLRDALLVAREAPERLVVNADRLPEVRTLAGRYGGEGLVAALESLSRARDALLRNGNARLVLD
VLMLDLHGAARF

Sequences:

>Translated_332_residues
MTWSAIRGQRMAVDLLRRAVAGGRVAHAYLFAGPAGVGKRLVALELARSLNCLEPRAGEACGACRSCLKLSAEPPVHPDV
TVVEPEGRMIRTEQMRQVQNEVYARPTEGRYKVVIIDGAERMNAESGNRLLKVLEEPPPYACFVLLTTNAAGVLPTIVSR
CQIVHFSPLPAEAIAAVLQEKAGIGPGEARLFASLSGGSVGAALAMAANPEVGRRRDEALSLLLRLGEMDDADLLEQAAA
WEKEKDHLEERLEMAAFWLRDALLVAREAPERLVVNADRLPEVRTLAGRYGGEGLVAALESLSRARDALLRNGNARLVLD
VLMLDLHGAARF
>Mature_331_residues
TWSAIRGQRMAVDLLRRAVAGGRVAHAYLFAGPAGVGKRLVALELARSLNCLEPRAGEACGACRSCLKLSAEPPVHPDVT
VVEPEGRMIRTEQMRQVQNEVYARPTEGRYKVVIIDGAERMNAESGNRLLKVLEEPPPYACFVLLTTNAAGVLPTIVSRC
QIVHFSPLPAEAIAAVLQEKAGIGPGEARLFASLSGGSVGAALAMAANPEVGRRRDEALSLLLRLGEMDDADLLEQAAAW
EKEKDHLEERLEMAAFWLRDALLVAREAPERLVVNADRLPEVRTLAGRYGGEGLVAALESLSRARDALLRNGNARLVLDV
LMLDLHGAARF

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=238, Percent_Identity=32.7731092436975, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787341, Length=315, Percent_Identity=29.2063492063492, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 35906; Mature: 35775

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWSAIRGQRMAVDLLRRAVAGGRVAHAYLFAGPAGVGKRLVALELARSLNCLEPRAGEA
CCCCHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHH
CGACRSCLKLSAEPPVHPDVTVVEPEGRMIRTEQMRQVQNEVYARPTEGRYKVVIIDGAE
HHHHHHHHHCCCCCCCCCCEEEECCCCCEECHHHHHHHHHHHHCCCCCCCEEEEEEECHH
RMNAESGNRLLKVLEEPPPYACFVLLTTNAAGVLPTIVSRCQIVHFSPLPAEAIAAVLQE
HCCCCHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHCEEEEECCCCHHHHHHHHHH
KAGIGPGEARLFASLSGGSVGAALAMAANPEVGRRRDEALSLLLRLGEMDDADLLEQAAA
HCCCCCCCCEEEEEECCCCHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
WEKEKDHLEERLEMAAFWLRDALLVAREAPERLVVNADRLPEVRTLAGRYGGEGLVAALE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCHHHHHHHHCCCCHHHHHHH
SLSRARDALLRNGNARLVLDVLMLDLHGAARF
HHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCC
>Mature Secondary Structure 
TWSAIRGQRMAVDLLRRAVAGGRVAHAYLFAGPAGVGKRLVALELARSLNCLEPRAGEA
CCCHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHH
CGACRSCLKLSAEPPVHPDVTVVEPEGRMIRTEQMRQVQNEVYARPTEGRYKVVIIDGAE
HHHHHHHHHCCCCCCCCCCEEEECCCCCEECHHHHHHHHHHHHCCCCCCCEEEEEEECHH
RMNAESGNRLLKVLEEPPPYACFVLLTTNAAGVLPTIVSRCQIVHFSPLPAEAIAAVLQE
HCCCCHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHCEEEEECCCCHHHHHHHHHH
KAGIGPGEARLFASLSGGSVGAALAMAANPEVGRRRDEALSLLLRLGEMDDADLLEQAAA
HCCCCCCCCEEEEEECCCCHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
WEKEKDHLEERLEMAAFWLRDALLVAREAPERLVVNADRLPEVRTLAGRYGGEGLVAALE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCHHHHHHHHCCCCHHHHHHH
SLSRARDALLRNGNARLVLDVLMLDLHGAARF
HHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]